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Citations for GE Life Sciences :
2651 - 2700 of 3218 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Alba proteins were run on an ion-exchange column (HiTrap SP column, 1 ml, GE Healthcare UK Ltd. Buckinghamshire, England) following the manufacturer’s protocol and as described previously (Maruyama et al. ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... The eluate was then diluted 3x with 20 mM Tris-HCl pH7.5 and passed over a 5 mL Q-sepharose column (GE Healthcare). The Flow through containing the MIF protein was then again loaded on a 5 mL CM sepharose column ...
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bioRxiv - Biochemistry 2020Quote: ... Sample was filtered through a 0.45 μm nitrocellulose filter and loaded onto a 5 ml HisWash1-equilibrated HisTrap HP column (GE Healthcare). 2 ml fractions were collected through a linear gradient over 100ml up to 100% HisElution [50 mM Tris ...
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bioRxiv - Biophysics 2020Quote: The protein extract was loaded onto a 1 mL HiTRAP Heparin column HP (GE Healthcare Life Sciences, 17-0406-01) equilibrated with Buffer A (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RBD was diluted to a concentration of 15 μg/mL with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units (RU) ...
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bioRxiv - Biophysics 2019Quote: ... The cleaved protein was then purified by a 5 ml HisTrap HP column on an AKTA Avant system (GE Healthcare). The flow through was combined and diluted with buffer C ...
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bioRxiv - Biochemistry 2020Quote: ... 250 μM TCEP and 20 mM sodium phosphate at pH 6.5 (ITC buffer) using a 5 mL HiTrap Desalting column (GE Healthcare). Likewise ...
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bioRxiv - Biophysics 2019Quote: ... The supernatant was filtered through a 0.22 μm or 0.45 µm filter depending on its viscosity and was loaded into a 10 ml TALON Sepharose resin (GE Healthcare) column ...
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bioRxiv - Biochemistry 2019Quote: ... The total eluate was diluted 1:2 with 25 mM Tris-HCl pH 8.0 and further purified by anion exchange chromatography using a 6 ml RESOURCE Q column (GE Healthcare) equilibrated in 95% AEX-A (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The HisTrap eluate was subsequently loaded on a Superdex 200 10/300 GL 24 ml gel filtration column (GE Healthcare), and eluted with buffer A plus 250 mM imidazole at a flow rate of 0.75 ml min−1 ...
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bioRxiv - Bioengineering 2020Quote: ... and supernatant was filtered with a 0.22 μm filter before loading onto a 1 ml HisTrap HP column (GE Healthcare). Proteins bound to the column were washed with 10 column volumes of washing buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: Purified His-tagged proteins anchor domain (2.1 mg/ml) was injected onto a Superdex 75 Increase 10/300 GL size exclusion column (GE Healthcare), equilibrated with 20 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... The VHH72-Fc constructs were purified from the clarified culture medium using a 5 ml MabSelect SuRe column (GE Healthcare). After a wash step with McIlvaine buffer pH 7.2 ...
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bioRxiv - Biochemistry 2021Quote: ... Precipitated DNA was pelleted by centrifugation and soluble lysate was loaded onto a 5 ml HisTrap HP column (GE Healthcare). The column was washed with 7 column volumes of loading buffer before being eluted with a gradient of imidazole (20–500 mM ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was filtered through a 0.22-μm filter and loaded onto a 5 mL His Trap HP column (GE Healthcare) pre-equilibrated with buffer A (10 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... The solution was filtered and the model degrons were loaded on a HiTrap SP HP 5 ml column (GE Healthcare) in 50 mM ammonium acetate pH 4.5 ...
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bioRxiv - Biochemistry 2021Quote: ... then the soluble fraction was filtered through 0.22 μm PVDF membrane before loading onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 20 column volumes of 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein-containing fractions were pooled and 1.6 ml protein was subjected to size exclusion chromatography using a Superdex 200 XK16/60 column (GE Healthcare) and equilibrated into 50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The medium from 1 L of culture was filtered using a 0.22-μm membrane and the filtrate was adjusted to contain 30 mM imidazole before incubating with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) beads for 2 h at 4 °C with mild agitation ...
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bioRxiv - Cell Biology 2020Quote: ... Sec13/31 and/or NHis-Sec13/31 aliquots were thawed and gel-filtrated on a 2.4 mL Superdex200 column (GE Healthcare) mounted on a ÄktaMicro (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... The eluate was subjected to size-exclusion chromatography on 125 ml Superdex 200 PG column (GE Healthcare Life Sciences, USA) in a buffer containing 50 mM NaH2PO4/Na2HPO4 pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were concentrated by ultrafiltration and buffer-exchanged into PBS on a 5 ml HiTrap Desalting column (GE Healthcare). The concentration was determined from UV absorbance at 280 nm ...
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bioRxiv - Microbiology 2020Quote: ... the harvested protein was concentrated to approximately 2.0 ml and filtered using a Superdex 200 gel filtration column (GE Healthcare) equilibrated with buffer (20 mM Tris-HCl and 200 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Clarified cell lysates (following 40 min centrifugation at 11,000 x g and 0.2 μm filtration) were loaded onto prepacked columns (1 ml) of Ni-Sepharose media (His trap, GE Healthcare) using a benchtop peristaltic pump ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified and the supernatant filtered through 0.22 μm filters and subjected to nickel affinity chromatography using 1 ml HisTrap HP columns (GE Healthcare). Elution was carried out via a 25-500 mM imidazole gradient in 50 mM Tris [pH 8] ...
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bioRxiv - Molecular Biology 2021Quote: ... MukBEF was largely found in the flowthrough and was applied to a 5 mL HiTrap Q HP column (GE Healthcare). The column was washed with 2 column volumes (CV ...
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bioRxiv - Immunology 2020Quote: ... Eluted protein was concentrated down to 2 mL for size exclusion chromatography with a Superose6 16/600 column (GE Healthcare) in 10 mM Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were incubated with 5 ml bed volume Glutathione Sepharose 4B resin (GE Healthcare, cat. No. 17-0756-05) for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... the clarified material was filtered with a 0.22 µm membrane and then applied a HisTrap HP 5 ml (GE Healthcare) and eluted with buffer B (30 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mM b-mercaptoethanol (BME) and then loaded onto a HiTrap SP HP 5 ml column (GE healthcare, Piscataway, NJ). Elution from the SP column was performed with a 70 ml linear gradient from 0–500 mM NaCl in SP buffer A ...
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bioRxiv - Cancer Biology 2022Quote: ... Human MSLN (Advance BioMatrix 5123-0.1mg) was (10 mg/mL) was immobilized onto a CM5 sensor chip (GE Healthcare, BR100012) by amine coupling ...
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bioRxiv - Cell Biology 2022Quote: ... was then loaded to a 5 mL HisTrap Ni-NTA column coupled to AKTA Pure protein purification system (GE Healthcare). The His-SUMO-tagged CAP1 protein was eluted from the nickel column with an imidazole gradient using buffer A and buffer B (buffer A + 250 mM imidazole) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a 0.45 μm syringe filter and loaded onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated in binding buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then concentrated down to 0.5 mL and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare) with MQA-5% glycerol without ATP ...
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bioRxiv - Biophysics 2022Quote: ... pH 7.5) for subsequent anion exchange chromatography on a 5 ml Mono-Q column (GE Healthcare Europe GmbH, Freiburg, Germany). Following an elution gradient with high-salt buffer (20mM TRIS ...
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bioRxiv - Biophysics 2022Quote: ... The eluent was concentrated to ~0.5 ml and then applied to the size-exclusion chromatography (SEC) column (Superose 6, 10/300, GE Healthcare) in the SEC buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... beads were washed once in lysis buffer and rotated at 4ºC overnight in wash buffer containing 8 U/ml thrombin (GE healthcare) to remove the GST tag ...
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bioRxiv - Molecular Biology 2022Quote: ... After passing through a 0.4 μm filter the supernatant was loaded onto a HisTrap HP 1 mL column (GE Healthcare). The protein was eluted with a lysis buffer supplemented with 300 mM Imidazole ...
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bioRxiv - Immunology 2022Quote: ... Nb-Fc was diluted to a concentration of 5 μg/ml with sodium acetate (pH 4.5) and immobilized on a CM5 chip (GE Healthcare) at a level of ∼150 response units ...
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bioRxiv - Biochemistry 2022Quote: ... Histones were then further purified by cation exchange on a 5 ml HiTrap SP HP column (GE Healthcare Life Sciences), pre-equilibrated with HGFB150 (full-length histones and tailless H2B) ...
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bioRxiv - Microbiology 2022Quote: ... coli supernatant was directly applied onto a 1-ml Ni2+ ion chelating poly-histidine affinity column (HisTrap HP, GE Healthcare) equilibrated with buffer A (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was transferred to ice cold 15 ml tubes containing 1ml of IgG Sepharose 6 Fast Flow (GE Healthcare) and incubated on a rotating platform for 3 hours at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant containing the 6xHis-tagged DMTases was stirred with 6 mL of NiCl2-charged chelating Sepharose beads (GE Healthcare) for 30 minutes at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 0.2 μm) and loaded with a flow rate of 1 ml/min onto a Source 15S 4.6/100PE column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Plant Biology 2022Quote: ... Additional purification was carried out by anion-exchange chromatography on a 1 mL HiTrap Q Sepharose column (GE Healthcare, USA). Yields of the recombinant-produced proenzymes ranged from 0.1 to 2 mg per litre culture ...
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bioRxiv - Cell Biology 2022Quote: ... The resultant supernatant was filtered using a 0.45 µm filter and the protein was subsequently purified by IMAC on a 5 ml HP HisTrap column (GE Healthcare) according to manufacturer’s information ...
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bioRxiv - Biochemistry 2023Quote: ... gel filtration with a flow rate of 0.8 mL/min using FPLC systems (AKTA pure 25, GE Healthcare Life Science). The 5 mL sample concentrated from the fractions involving the target proteins with Amicon Ultra-15 10 kDa was loaded on Resource Q (GE Healthcare Life Science ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ml antibody-containing ascites was incubated with 1.2 ml settled bead volume of pre-equilibrated Protein G Sepharose Fast Flow beads (GE Healthcare) for 2 hr at 4°C with gentle agitation ...