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Citations for GE Life Sciences :
2651 - 2700 of 5577 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the holo-proteins were then loaded onto a Superdex 75 Increase 10/300 GL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Cancer Biology 2019Quote: ... The proteins were then transferred to a 0.45-μm PVDF membrane (Amersham Hybond, GE Healthcare, München, Germany) which was blocked in 2% bovine serum albumin (Amresco ...
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bioRxiv - Molecular Biology 2019Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... a frozen protein aliquot was thawed and loaded onto a Superdex HR 75 10/300 (GE Healthcare) size exclusion column and eluted with 150 mM NaCl ...
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Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infectionbioRxiv - Cell Biology 2020Quote: ... proteins were further concentrated with filters and then purified by anion exchange chromatography on HitrapQ (GE Healthcare) and collected fractions were further loaded onto size exclusion column (Superdex 75 16/60 ...
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bioRxiv - Plant Biology 2019Quote: ... The expressed protein domains were purified via Ni2+ affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The eluted proteins were subjected to buffer change by PD10 desalting column into ITC-buffer (25 mM BisTris-HCl pH6.0 ...
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bioRxiv - Microbiology 2019Quote: ... Dual-labeled capsid proteins were separated using a PD Mini-Trap G-25 desalting column (GE healthcare) with imaging buffer (50 mM Tris ...
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bioRxiv - Pathology 2020Quote: ... Recombinant proteins were purified with a Ni-NTA resin and Q-Sepharose™ (GE Healthcare, Mississauga, ON). Endotoxin was removed using the Pierce™ High-Capacity Endotoxin Removal Resin (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... Size-exclusion chromatography was performed by loading the protein onto Superose 6 Increase 10/300GL (GE Healthcare) column with running buffer containing 20 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant containing soluble proteins was collected and allowed to flow through Ni-NTA resin (GE Healthcare). After washing with three column volumes of sonification buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The cleaved protein was purified further using a Superdex Increase 75 10/300 (GE Healthcare Life Sciences), pre-equilibrated with 20 mM Hepes pH 7.5 and 150 mM NaCl.
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bioRxiv - Biophysics 2020Quote: ... The plexin protein in the supernatant was captured using a 1 mL HisTrap FF column (GE Healthcare) and eluted by Buffer B containing 10 mM Tris (pH 8.0) ...
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bioRxiv - Plant Biology 2020Quote: The GST- and MBP-fusion proteins were separately purified using Glutathione sepharose (GE Healthcare, 17-5132-01) and Amylose resin (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged proteins were immobilized on 100 μl Glutathione–Sepharose beads in 1xPBS (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was buffer exchanged to remove imidazole using a 16/260 HiLoad desalting column (GE healthcare). BpsDsbA was then incubated with TEV protease in a 1:50 (TEV:BpsDsbA ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant protein MdSUMO2A-HIS and MdDREB2A were purified by HIS Sepharose beads (GE Healthcare, Fairfield, CT, USA), GST-MdCE was purfied by Pierce™ Glutathione Spin Columns (16105 ...
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bioRxiv - Molecular Biology 2021Quote: ... SEC was carried out by loading the protein sample to Superdex 200 Increase 10/300GL (GE Healthcare) to obtain the monomer complex ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was loaded onto a 1 ml HiTrap Heparin HP column (GE Healthcare, 17-0406-01) and eluted with a linear gradient from buffer A (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were purified using Affinity chromatography purification on Glutathione Sepharose 4B beads affinity column (GE Healthcare). After that ...
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bioRxiv - Microbiology 2021Quote: ... the proteins were subjected to a size exclusion chromatography using a Superdex 200 Increase column (GE Healthcare) with a flow rate of 0.5 ml/min using buffer B ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were further purified by size exclusion chromatography on a HiLoad 16/60 Superdex 200 (GE Healthcare) column using buffer III and a flowrate of 1 mL/min ...
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bioRxiv - Biochemistry 2021Quote: ... All columns were calibrated using protein standards from both high and low molecular weight kits (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... Eluted protein was further purified using an anion exchange column (HiTrap Q HP 5 ml; GE Healthcare) before buffer exchange into PBS and storage at –20°C.
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were diluted to 75 mM NaCl and loaded onto a MonoQ 5/50 GL (GE Healthcare) equilibrated in QO buffer (20 mM Tris pH 8 ...
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Adaptation of the Romanomermis culicivorax CCA-adding enzyme to miniaturized armless tRNA substratesbioRxiv - Molecular Biology 2020Quote: ... Protein-containing fractions were combined and concentrated on Vivaspin 6 columns (15-30 kDa MWCO, GE Healthcare). Proteins were stored in 40 % glycerol (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Binding of GAPDH proteins to EV surface was monitored by UV-280 chromatogram (Akta pure, GE Healthcare), western blotting ...
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bioRxiv - Neuroscience 2020Quote: ... The concentrated protein was injected into the AKTA purifier Fast Performance Liquid Chromatography (FPLC) (GE Healthcare, USA) and further purified using Superdex 75 column in 50 mM sodium phosphate buffer (pH 7.0) ...
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bioRxiv - Immunology 2020Quote: ... the supernatants with the secretion of antibodies were collected and captured by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and dialyzed into phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins were purified by nickel affinity chromatography using HisTrap Column (GE Healthcare Life Sciences Pittsburgh, PA). Rat polyclonal antisera against L ...
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bioRxiv - Immunology 2019Quote: ... Proteins were separated by 8% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare, Bucks, UK) using the Trans-Blot SD Semi-Dry Transfer system (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were purified by SEC (size-exclusion chromatography) on HiLoad 26/600 Superdex 75 column (GE Healthcare) in 25 mM sodium phosphate pH 6.4 with 100 mM NaCl for hPD-1 or in PBS pH 7.4 for hPD-L1 ...
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bioRxiv - Cell Biology 2019Quote: ... The CiEB1-mCherry proteins expressed with histidine tag were purified using a Ni-NTA column (GE Healthcare) and then the solvent was exchanged with distilled water by using NAP-5 column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... The concentrated protein was injected into prepacked HiLoad 16/600 Superdex 200 Prep Grade column (GE Healthcare), equilibrated with 2 Column Volume (CV ...
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bioRxiv - Cell Biology 2019Quote: ... Protein solution was then subjected to size-exclusion chromatography using a HiLoad Superdex 200 column (GE Healthcare) in 50 mM HEPES NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... and the remaining BMAL1 PAS-AB protein was further purified by Superdex75 gel filtration chromatography (GE Healthcare) into 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Affinity purified protein was further purified by size-exclusion chromatography (16/60 Superdex 75 PG, GE Healthcare) in the gel filtration buffer (20 mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed (6×5 min) with TBS-T and proteins were visualized by quimioluminiscence (GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples (400 μL injection volume) were separated using a Superdex 200 10/300 column (GE Healthcare) equilibrated with buffer (50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were separated using a Superdex 75 Increase 10/300 GL gel filtration column (GE Healthcare) connected to an LC-10 Prominence HPLC system (Shimadzu) ...
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bioRxiv - Cell Biology 2019Quote: ... and eluted proteins were further purified by size exclusion chromatography using a Superdex 75 column (GE Healthcare) into a buffer containing 25 mM HEPES ...
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bioRxiv - Immunology 2020Quote: ... Sera were then pooled for each group and IgGs were purified using protein G beads (GE Healthcare). The IgG concentration for each sample was obtained measuring the absorbance at 280 nm using a nanodrop (Thermo Scientific).
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bioRxiv - Biochemistry 2021Quote: ... Protein fractions were then loaded on a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare), and eluted with 10 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... and electrophoresed proteins were transferred to an Amersham Protran Premium 0.45 µm nitrocellulose membrane (GE Healthcare 10600096). The membrane was then blocked (1% skim milk in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was injected onto Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare, USA) equilibrated in Assay buffer (20 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was injected onto Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare, USA) equilibrated in Assay Buffer (20 mM HEPES pH 7.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Then Cas13 proteins were subjected to a final polishing step by gel filtration (Sephacryl 200, GE Healthcare). The purity of >95% was assessed by SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the Sirh3-Venus fusion protein was detected with an ECL Prime Western Blotting Detection kit (GE Healthcare) using an anti-GFP antibody (MBL ...