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Citations for GE Life Sciences :
2651 - 2700 of 3304 citations for 8 1 Methoxyethoxy 2 6 dimethyloct 2 ene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM Tris pH 7.2 ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... An enhanced chemiluminescence (ECL)-horse radish peroxidase (HRP) conjugated secondary antibody (NA934V, 1:1000 dilution, GE Healthcare, Uppsala, Sweden) and SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... cells were harvested and rAAVs were purified using 1 mL HiTrap heparin columns (GE Healthcare Bio-Sciences, Uppsala, SwedenSigma) and concentrated using Amicon Ultra centrifugal filter devices (Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL, GE Healthcare, Chicago, IL). Polyclonal affinity purified NS3 and NS5 proteins were produced as previously described (20) ...
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bioRxiv - Immunology 2021Quote: ... IgGs were purified from ~1 ml of plasma with equal volume of settled Protein A Sepharose resin (GE Healthcare). Samples were eluted from the resin with 0.1 M glycine at pH 2.5 and immediately neutralized with 1 M Tris-HCl pH 8 ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant proteins were then purified using a 1 ml HisTrap HP column and ÄKTA™ system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting soluble fraction was applied on a Ni-NTA column (GE Healthcare, His Trap FF 1 ml) and purified according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... MERS-CoV NTD and SARS-CoV-1 NTD were then further purified using a Superdex 75 column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Chromosomal DNA fragments were separated on a 1% agarose gel and blotted onto Hybond N+ nylon membranes (GE Healthcare), following standard techniques (Sambrook and Russell ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
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bioRxiv - Biophysics 2021Quote: ... and MtCNNMCorC (residues 333-417) were subcloned into BamHI and XhoI sites of pGEX-6P-1 vector (GE Healthcare) with an N-terminal GST-tag.
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bioRxiv - Biophysics 2020Quote: ... Protein fractions (eluted at ~ 1 M NaCl) were then purified using a HiLoad 16/60 Superdex 75 (GE Healthcare) column (SEC ...
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bioRxiv - Biochemistry 2022Quote: ... 100 µL TIR-SAVED (10.9 mg.mL-1) were injected into the Superose 12 column 10/300 GL (GE Healthcare) in 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 mM DTT] and filtered on a Superdex 200 10/300 GL column (GE Healthcare, Little Chalfont, UK). Aliquots of the protein were flash-frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity was detected using a horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (IgG, 1:10,000, NA934 GE Healthcare). Tau13 and Tau5 immunoreactivity were detected using an anti-mouse IgG (1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... Ten μl of each culture were spotted onto 15 mm grade 1 Whatman filter discs (GE Healthcare Life Sciences). After drying for 1 minute at room temperature ...
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bioRxiv - Biochemistry 2021Quote: A sample of nanodiscs reconstituted LicB:sybody-C complex was concentrated between 1 to 1.5 mg/ml using a Vivaspin concentrator with a 30,000 Da cutoff (GE Healthcare). Cryogenic samples were prepared using a Mark IV Vitrobot (Thermo Fisher ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1 mM DTT) and purified by size-exclusion chromatography in a HiLoad 26/100 Superdex 200 column (GE Healthcare) pre-equilibrated with gel filtration buffer ...
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bioRxiv - Cell Biology 2019Quote: ... primary antibody and horseradish peroxidase linked anti-rabbit IgG (from donkey) secondary antibody (1:5000, GE Healthcare Life Sciences). Signal was detected by chemiluminescence (Luminata Forte Western HRP substrate ...
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bioRxiv - Systems Biology 2019Quote: ... 0.7% (v/v) acetonitrile (ACN) and 1% (v/v) carrier ampholytes mixture (IPG buffer pH 3.0-10.0, GE Healthcare). Fractionation was performed on a 3100 OFFGEL (OGE ...
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bioRxiv - Biophysics 2020Quote: ... The untagged proteins were further purified and concentrated using 1 mL HiTrapTM Heparin HP columns (GE Healthcare Life Sciences). The eluted proteins were analyzed on 10% SDS-PAGE gels and pure fractions (>95% purity ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated at 37 °C for 1 h and then passed through G-25 spin columns (GE Healthcare) according to the manufacturer’s instructions to remove unincorporated radioactivity ...
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bioRxiv - Biochemistry 2019Quote: ... The water phase was loaded to a MonoQ 5/50 GL (1 ml) column (GE17-5166-01, GE Healthcare). ppGpp was eluted with a linear gradient from 0.5 mM to 600 mM LiCl in buffer C (25 mM Tris-HCl pH 8.3 ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM EGTA) supplemented with 50 mM KCl and was further purified using an AKTA FPLC system (GE Healthcare) with a Superdex 200 10/300 GL (Amersham Biosciences ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% CO2 and maintained in a 4:1 ratio of DMEM (Dulbecco’s modified Eagle’s medium, GE Healthcare, Logan, UT) to Medium 199 containing 10% calf serum (HyClone ...
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bioRxiv - Biochemistry 2021Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Biochemistry 2021Quote: ... to <1 mL and injected onto a Superdex™ 75 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20mM Tris pH 8.5 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The filtered solution was then purified by Ni2+ affinity chromatography using a 1 mL HisTrap FF column (GE Healthcare). The column was equilibrated with 25 mL Buffer A (20 mM sodium phosphate (pH 7.4) ...
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bioRxiv - Microbiology 2021Quote: All imaging was done on 1% CDM agarose pads with a Deltavision Elite Deconvolution Microscope system (GE Healthcare Biosciences) with an environmental chamber maintained at 37 °C with a beaker of water to maintain humidity ...
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bioRxiv - Molecular Biology 2021Quote: ... 80-500 mM imidazole fractions were combined and applied directly to a HiTrap Heparin HP (1 mL) (GE Healthcare) column ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... 100 μL of protein sample at 1 mg/mL was injected onto an S200 10/300 column (GE Healthcare) equilibrated in PBS ...
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bioRxiv - Immunology 2020Quote: ... 3-fold serial dilution (spanning a range of 1 μM to 1.4 nM) in HBS-EP buffer (GE healthcare). A high flow rate of 40 μL/min was used ...
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bioRxiv - Cell Biology 2020Quote: ... An 11 kb Spe1-Sal1 fragment (mid exon 1–7) was labelled with Cy3-dUTP (GE Healthcare UK Ltd) using Bioprime labelling kit (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... for between 1 - 4 days and the protein signal visualized using a Typhoon Trio Variable Mode Imager (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
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bioRxiv - Biochemistry 2022Quote: ... to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare) equilibrated in gel filtration buffer ...
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bioRxiv - Microbiology 2022Quote: ... For all primary mouse antibodies an anti-mouse-HRP in a dilution of 1:2000 was used (GE Healthcare). All blots were blocked with TBS-T 5% Milk and washed 3x for 15 minutes with TBS-T after each antibody incubation ...
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... and then purified through affinity chromatography using a 1 mL HiTrap Protein G HP column (GE Healthcare Life Sciences). Antibodies were eluted from the column using 0.1 M glycine-HCl ...
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bioRxiv - Synthetic Biology 2022Quote: ... The filtered solution was then purified by Ni2+ affinity chromatography using a 1 mL HisTrap FF column (GE Healthcare). The column was equilibrated with 25 mL Buffer A at a constant flow rate of 1 mL/min ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (1-5 mg/ml) were loaded individually on a Superdex 200 Increase 10/300 column (GE Healthcare), and the elution profiles were obtained using a tandem of sequentially connected UV-Vis Prostar 335 (Varian ...
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bioRxiv - Microbiology 2024Quote: ... MV samples were administered at six concentrations (1:3 dilutions in Neurobasal A (Hyclone, GE Healthcare, Chicago, IL, USA) containing Glutamax (Hyclone ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble extract was loaded onto the Hi-Trap Chelating HP 1 ml pre-packed column (GE Healthcare/Cytiva) pre-equilibrated with buffer A containing 500 mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... was used at 1:10,000 and proteins were visualised by enhanced chemiluminescence using the ECL-Plus system (GE Healthcare).
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bioRxiv - Immunology 2024Quote: NP constructs (each at 100 μg) were loaded separately on 1 ml heparin column (GE17-0407-01, GE Healthcare) pre-equilibrated with buffer A (20mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences, Marlborough MA) at a 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... TbSmee1(1-400) concentrates were applied to a previously equilibrated HiLoad 16/600 Superdex 200 pg column (GE Healthcare) pre-equilibrated in dialysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated anti-rat or anti-mouse (1:2000; GE healthcare) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and the supernatant was filtered through 0.45 µm filters before loading onto a 1 mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A (100 mM Tris-HCl [pH8] ...