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Citations for GE Life Sciences :
2551 - 2600 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... The proteins were visualized by a chemiluminescence method (Amersham Imager 600, GE healthcare Life Sciences, Buckinghamshire, UK). Protein signals were quantified using ImageJ software (National Institute of Health ...
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bioRxiv - Plant Biology 2023Quote: ... Two mg of proteins in buffer were mixed with 50ul 7-Methyl-GTP-Sepharose 4B (GE Healthcare) previously equilibrated with extraction buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The cleaved proteins were separated by a 16/600 Superdex75 gel filtration column (GE Healthcare, Chicago, lL), followed by depleting the peak of lgMPZ peak or residual MBP by by applying to amylose resin ...
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bioRxiv - Biophysics 2022Quote: ... The dialyzed protein solution (∼50 μM) was then loaded into the sample cell in iTC200 (GE Healthcare) after the removal of insoluble materials by centrifugation (10,000×g ...
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bioRxiv - Cell Biology 2022Quote: ... cleared protein was subjected to size-exclusion chromatography using a HiLoad 16/600 Superdex column (GE Healthcare) in lysis buffer ...
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bioRxiv - Microbiology 2022Quote: Total IgG was purified from the final bleed rabbit sera using Protein G Gravitrap (GE Healthcare, Cytiva). The sera were diluted as 1:5 in binding buffer (20 mM Sod ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated on a 12% SDS-PAGE gel and transferred to a nitrocellulose membrane (GE Healthcare), which was then inucbated over-night with primary antibodies followed by a 1-hour incubation with fluorescent secondary antibodies (Table 1) ...
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bioRxiv - Biophysics 2024Quote: ... the protein sample was passed through a Hi-Trap Q-Sepharose FF crude (GE Healthcare, Piscataway, NJ) column ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins of interest were detected using Chemiluminescence by adding Amersham Enhanced chemiluminescence (ECL) detection reagent (GE healthcare). For quantification of Yops-secretion ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified from soluble fractions of the bacterial cell lysates using Glutathione Sepharose beads (GE Healthcare). Proteins were dialyzed in 1x TBS overnight at 4°C overnight and protein quantified ...
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bioRxiv - Cell Biology 2024Quote: ... and guinea pig anti-Rad21 and guinea pig anti-Pds5b coupled to protein A sepharose (GE Healthcare). For non-covalent coupling of antibodies to beads ...
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bioRxiv - Molecular Biology 2024Quote: ... The recovered proteins after cleavage underwent size exclusion chromatography using a Superdex S75/300 column (GE Healthcare) in phosphate-based buffer [200 mM sodium phosphate pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.1LmM PMSF) and included 80 pmol of GST-fused protein immobilized on GSH beads (GE Healthcare), 1–8 µg of heparin sulfate ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation was performed using the described antibodies and protein G- or A- coupled Sepharose beads (GE Healthcare). Beads were washed with high salt buffer (50 mM Tris/Cl ...
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bioRxiv - Microbiology 2023Quote: Protein samples were separated on 10–15% polyacrylamide gels and transferred to Amersham nitrocellulose membranes (GE healthcare) by Trans-blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by SEC using a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated with Buffer B ...
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bioRxiv - Biochemistry 2022Quote: ... Protein purity was assessed by SDS-polyacrylamide gel electrophoresis (PhastGel Homogenous 20 pre-cast gels, GE Healthcare) and the enzyme concentration ([E] ...
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bioRxiv - Biochemistry 2022Quote: ... For crystallography proteins were purified further via size-exclusion chromatography (HiLoad 16/600 Superdex 200, GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were pre-incubated with 60 uL of protein A-Agarose beads (GE Healthcare, Chicago, USA) + Salmon Sperm DNA (Invitrogen #15632-011 ...
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bioRxiv - Microbiology 2022Quote: Proteins were purified by immobilized metal affinity chromatography (IMAC) using Ni-Sepharose excel resin (GE Healthcare, USA). The column was first washed with 5 column volumes of loading buffer (50 mM tris(hydroxymethyl)aminomethane (TRIS) ...
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bioRxiv - Microbiology 2022Quote: ... cell culture media were clarified by centrifugation and the IgG captured using Protein A resin (GE Healthcare). Proteins were eluted from the resin using citrate buffer pH 3 ...
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bioRxiv - Biochemistry 2023Quote: ... The dialyzed proteins were concentrated and purified by SEC (HiLoad Superdex-200 16/600 column, GE Healthcare) into tau buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The eluted proteins were concentrated and loaded onto a Superdex 200 column (10/300 GL; GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... wet electroblotting (100V for 1h) was used to transfer proteins onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). After blocking (blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were immobilized by blotting them onto an Amersham Protran Premium nitrocellulose membrane (GE Healthcare Life Sciences) in cold 1 x Tris-Glycine ...
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bioRxiv - Plant Biology 2023Quote: ... and the eluted proteins were further purified via gel filtration chromatography with a superdex 200 (GE Healthcare) column ...
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bioRxiv - Microbiology 2023Quote: ... The pull-down of biotinylated proteins was performed using 100 µl StrAv Mag Sepharose™ (GE Healthcare) for each condition and incubated in a rotation wheel for 3h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by nickel affinity chromatography using a 5 ml HisTrap HP column (GE Healthcare). The eluted proteins were dialyzed into 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was transferred to an Amersham™ Hybond™ P0.45 polyvinylidene difluoride (PVDF) membrane (GE Healthcare) in 1x Tris/Glycine transfer buffer (National diagnostics ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified using an anion exchange chromatography column (HiTrap Q HP, 5 mL; GE Life Sciences) followed by ammonium sulfate precipitation (20 to 30% ammonium sulfate saturation ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were pooled and concentrated before separation on a Superdex 200 16/60 column (GE Healthcare) equilibrated in GF2 Buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then purified using a ResourceTM Q 6ml column (17-1179-01, GE Healthcare, Uppsala, Sweden) and a Superdex 200 Increase 10/300 column (28-9909-44 ...
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bioRxiv - Genetics 2023Quote: ... Protein containing fractions were further purified by size exclusion chromatography using Superdex 200 10/300 (GE Healthcare) gel-filtration column in SEC buffer (20 mM HEPEs pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were concentrated and gel-filtrated on a SuperdexTM 75 size exclusion chromatography column (GE Healthcare) equilibrated with stock buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Bioengineering 2023Quote: ... The target protein was purified by loading the supernatant onto a prepacked His SpinTrap column (GE Healthcare) and eluting with a buffer containing 20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples with indicated concentrations were loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... The cleaved protein was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare, USA) pre-equilibrated with gel filtration buffer (150 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... The filtered supernatant included NMnPP protein was applied to a nickel-nitrilotriacetic acid (Ni-NTA, GE Healthcare) affinity column pre-equilibrated with buffer A ...
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bioRxiv - Immunology 2023Quote: ... The secreted antibodies in the supernatant were purified by protein G Sepharose 4 Fast Flow (GE Healthcare). Purified antibodies were dialyzed in PBS and sterile filtered (0.22 μm) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS/PAGE gel and transferred to Protran nitrocellulose membrane (0.2 μm, GE Healthcare). Immunoblotting was performed by incubating with primary antibodies diluted in Tris-buffered saline (20mM Tris pH 7.6 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare) in NETN buffer (20mM Tris PH8 ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the protein was cleaved with Tev protease and purified on a HiTrap Q HP column (GE Healthcare) using a salt gradient ...
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bioRxiv - Biochemistry 2024Quote: ... 200 µL of protein was injected into the Superdex 75 or Superdex200 size exclusion column (GE Healthcare) (volume less than 900 µL for preparative runs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Acetylated LCas12a proteins were purified following the steps of Ni-NTA column (HisTap™ HP, GE Healthcare), ion exchange chromatography (HiTrap™ SP HP ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified using nickel affinity chromatography with a 5 ml HisTrap HP column (GE Healthcare) followed by size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein was passed through a HiLoad® 26/600 Superdex® 75 pg (GE Healthcare) size exclusion chromatography column and the resulting pure protein was stored in gel filtration buffer ...
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bioRxiv - Biochemistry 2023Quote: Proteins were analyzed on a Superdex 200 10/300 GL column from GE healthcare (Chicago, IL, USA) equilibrated using a mobile phase containing 150 mM KCl and 50 mM Tris-HCl pH 7.6 run at 0.4 ml/min ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...