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Citations for GE Life Sciences :
2551 - 2600 of 4264 citations for Recombinant Human S100A12 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The concentrated protein was injected into the AKTA purifier Fast Performance Liquid Chromatography (FPLC) (GE Healthcare, USA) and further purified using Superdex 75 column in 50 mM sodium phosphate buffer (pH 7.0) ...
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bioRxiv - Immunology 2020Quote: ... the supernatants with the secretion of antibodies were collected and captured by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and dialyzed into phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Immunology 2019Quote: ... Proteins were separated by 8% SDS-PAGE and transferred to Protran nitrocellulose membranes (GE Healthcare, Bucks, UK) using the Trans-Blot SD Semi-Dry Transfer system (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were purified by SEC (size-exclusion chromatography) on HiLoad 26/600 Superdex 75 column (GE Healthcare) in 25 mM sodium phosphate pH 6.4 with 100 mM NaCl for hPD-1 or in PBS pH 7.4 for hPD-L1 ...
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bioRxiv - Cell Biology 2019Quote: ... The CiEB1-mCherry proteins expressed with histidine tag were purified using a Ni-NTA column (GE Healthcare) and then the solvent was exchanged with distilled water by using NAP-5 column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... The concentrated protein was injected into prepacked HiLoad 16/600 Superdex 200 Prep Grade column (GE Healthcare), equilibrated with 2 Column Volume (CV ...
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bioRxiv - Cell Biology 2019Quote: ... Protein solution was then subjected to size-exclusion chromatography using a HiLoad Superdex 200 column (GE Healthcare) in 50 mM HEPES NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... and the remaining BMAL1 PAS-AB protein was further purified by Superdex75 gel filtration chromatography (GE Healthcare) into 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Affinity purified protein was further purified by size-exclusion chromatography (16/60 Superdex 75 PG, GE Healthcare) in the gel filtration buffer (20 mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were washed (6×5 min) with TBS-T and proteins were visualized by quimioluminiscence (GE Healthcare Amersham™ ECL™ Western Blotting Detection Reagents ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples (400 μL injection volume) were separated using a Superdex 200 10/300 column (GE Healthcare) equilibrated with buffer (50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were separated using a Superdex 75 Increase 10/300 GL gel filtration column (GE Healthcare) connected to an LC-10 Prominence HPLC system (Shimadzu) ...
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bioRxiv - Cell Biology 2019Quote: ... and eluted proteins were further purified by size exclusion chromatography using a Superdex 75 column (GE Healthcare) into a buffer containing 25 mM HEPES ...
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bioRxiv - Immunology 2020Quote: ... Sera were then pooled for each group and IgGs were purified using protein G beads (GE Healthcare). The IgG concentration for each sample was obtained measuring the absorbance at 280 nm using a nanodrop (Thermo Scientific).
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bioRxiv - Biochemistry 2021Quote: ... Protein fractions were then loaded on a HiLoad 16/60 Superdex 200 gel filtration column (GE Healthcare), and eluted with 10 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... and electrophoresed proteins were transferred to an Amersham Protran Premium 0.45 µm nitrocellulose membrane (GE Healthcare 10600096). The membrane was then blocked (1% skim milk in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was injected onto Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare, USA) equilibrated in Assay buffer (20 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was injected onto Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare, USA) equilibrated in Assay Buffer (20 mM HEPES pH 7.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Then Cas13 proteins were subjected to a final polishing step by gel filtration (Sephacryl 200, GE Healthcare). The purity of >95% was assessed by SDS-PAGE ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the Sirh3-Venus fusion protein was detected with an ECL Prime Western Blotting Detection kit (GE Healthcare) using an anti-GFP antibody (MBL ...
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bioRxiv - Biophysics 2021Quote: ... protein samples were loaded onto a size exclusion chromatographic column (Superdex200 Increase 10/300 GL, GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... followed by fast protein liquid chromatography on the ÄKTA pure system (GE Healthcare Life Sciences, Chicago, IL) using protein A as the first chromatography step and ceramic hydroxyapatite (CHT ...
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bioRxiv - Biophysics 2020Quote: ... proteins were further purified with size exclusion chromatography (SEC; Superdex 200 Increase 10/300 GL, GE Healthcare) in running buffer containing 25 mM glycine ...
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bioRxiv - Microbiology 2021Quote: ... The protein solution was then loaded onto a Superdex 10/60 Hiload size exclusion column (GE Healthcare) equilibrated with buffer C ...
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bioRxiv - Microbiology 2021Quote: ... The protein was further purified by size exclusion chromatography (Superdex 200 Increase, GE Healthcare Biosciences, Piscataway, NJ) in TBS (137 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The dialysed protein was further purified using a MonoQ 10/30GL anion exchange chromatography column (GE Healthcare) by a linear gradient of NaCl with buffer D and buffer E ...
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bioRxiv - Microbiology 2020Quote: The heterologous proteins containing His-tags were purified via 1 ml Ni-NTA HisTrap columns (GE Healthcare). The cells were lysed in 50 ml lysis buffer containing 50 mM Na-phosphate buffer pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... 0.03 % Triton-X 100) and proteins were eluted directly into a 1 mL HisTrap column (GE Healthcare) using purification buffer supplemented with Desthiobiotin 2.5 mM (IBA Solutions For Life Science™) ...
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bioRxiv - Molecular Biology 2021Quote: ... To isolate activated protein C (aPC) ion exchange chromatography with FPLC (ÄKTAFPLC®, GE Healthcare Life Sciences) was used ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were purified by Ni-NTA IMAC using Ni-Sepharose 6 Fast Flow resin (GE Healthcare). The wash buffer consisted of 50 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Anti-DEC205-OVA was purified from the media using a HiTrap Protein G HP column (GE Healthcare) according to the manufacturer’s recommendation ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... protein extract was fractionated by anion exchange chromatography using a MonoQ 5/50 GL column (GE Healthcare), washed with 10 column volumes (CV ...
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bioRxiv - Immunology 2021Quote: ... the supernatants with the secretion of antibodies were collected and captured by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and dialyzed into phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2020Quote: ... Post-nuclear supernatants were incubated with 3 μg Protein A Sepharose CL-4B (PAS; Ge HealthCare, #GEH17078001) for 1 hour and then with 3 μg PAS plus primary antibody (0.4 ug anti-GFP mAb ...
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bioRxiv - Immunology 2020Quote: ... Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... The purified protein was run over a 10/300 Superose 6 size-exclusion column (GE Life Sciences). Fractions containing the trimeric HPIV3 F proteins were pooled and concentrated by centrifugation in an Amicon ultrafiltration unit (Millipore ...
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bioRxiv - Immunology 2021Quote: ... The proteins were purified further by gel filtrations through a Superdex 200 26/60 column (GE Healthcare) equilibrated with gel filtration buffer ...
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bioRxiv - Immunology 2021Quote: ... IFN-α2 protein was column-purified using Nap-5 columns (GE Healthcare, Chicago, IL; #17-0853-01), incubated with 2.5ul serum ...
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bioRxiv - Immunology 2020Quote: ... 5-10 μg denaturated proteins were resolved by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Antibodies against HOIL1 (H-1 ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Cy5-labelled proteins were purified by size-exclusion chromatography (SEC) on a Superdex 200 Increase (GE Healthcare) column with PBS and concentrated on a 30 kDa Amicon Tube ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... Labelling of the proteins was verified by measuring OD633 during SEC on the Akta Explorer (GE Healthcare). The final concentration was determined by OD280 measurement on the Nanodrop and verified by SDS-PAGE.
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bioRxiv - Immunology 2021Quote: ... The eluted protein was further purified using a Superdex 200 Increase 10/300 GL column (GE Healthcare). The purity and size of the protein were analyzed by sulphate-polyacryl-amide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Cell Biology 2021Quote: ... The protein was purified from cleared cell lysate using Ni-sepharose 6 Fast flow resin (GE Healthcare) in a poly-prep column (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated in centrifugal concentrators and purified by size-exclusion chromatography (Superdex 200; GE Life Sciences) in gel filtration buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... and protein bands were visualised via chemiluminescence using an ECL Plus Western blotting detection kit (GE Healthcare) and an LAS-4000 imager (Fujifilm) ...
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bioRxiv - Biophysics 2020Quote: ... The concentrated protein sample was loaded on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with GF buffer (50 mM TRIS pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... The protein conjugate was purified on a Superdex 200 increase 10/30 gL column (GE Healthcare, US) with a flow rate of 0.5 mL/min with PBS as elution buffer ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... Proteins were further purified by gel filtration chromatography (Superdex-75) using AKTA pure FPLC system (GE Healthcare) with a buffer containing 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was then concentrated and loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES pH 7.4 ...