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Citations for GE Life Sciences :
2551 - 2600 of 2936 citations for 17 Hydroxyprogesterone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The sample (100 µL of 1 mg/mL UL21-H6) was injected onto a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated in 20 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Binding was detected by incubating the plates sequentially with either mouse anti-Histidine Tag antibody (MCA1396, Abd Serotec) followed horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2000, NXA931, GE Healthcare) or Streptavidin-HRP (554066 ...
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bioRxiv - Plant Biology 2021Quote: ... Purification of the unprocessed form of cardosin B was performed by IMAC using a 1 mL HisTrap column (GE Healthcare, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare). After washing with 20 ml lysis buffer containing 8M urea ...
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bioRxiv - Microbiology 2020Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... and fractionated by linear gradient with end buffer (10 mM Tris-HCl, 1 M NaCl) using an AKTAprime plus (GE Healthcare) at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Fifty microliters of the purified RXFP1(1-72) and RXFP2(1-65) at 5 mg/ml was injected over a Superose 6 5/150 increase column (GE Healthcare) equilibrated and eluted with TBS (pH 7.4 ...
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bioRxiv - Cell Biology 2020Quote: Yeast Cka1 and Tda1 genes were amplified by PCR from yeast genomic DNA and then cloned into pGEX4T-1 (GE healthcare) vector ...
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bioRxiv - Cell Biology 2021Quote: ... eluted in buffer F containing 200 mM imidazole and further purified over a 1 ml ResourceS cation exchange chromatography column (GE Healthcare) using a 20 ml gradient from 100 mM to 500 mM NaCl in 50 mM 2-(N-morpholino)ethanesulfonic acid (MES ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with HRP-coupled secondary antibodies (santa cruz, cell signaling) for 1 h at RT and chemiluminescence detected with ECL Prime (GE Healthcare) at the LAS-1000 (Fujifilm) ...
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bioRxiv - Cell Biology 2022Quote: ... incubated in HRP conjugated secondary antibodies detailed in Table 4 for 1 h at room temperature and developed using Pierce SuperSignal Pico Plus (Pierce) or ECL (GE Healthcare) reagent and imaged on ImageQuant.
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bioRxiv - Biophysics 2022Quote: ... Peak fractions were diluted with 2 volumes of buffer H and loaded onto a 1-mL SP HP column (GE Healthcare) and washed with buffer C (50 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The coding sequences for the N-terminal (63-139 aa) and C-terminal (221-307 aa) domain of OR were cloned to the pGEX-4T-1 vector (GE healthcare) for prokaryotic expression ...
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bioRxiv - Molecular Biology 2022Quote: ... 80 µg of a 5' amino-modified DNA oligonucleotide (Table S1) complementary to target tRNA (IDT) was covalently coupled to a HiTrapTM NHS-activated HP column (1 ml, GE Healthcare). Binding to the NHS column was performed by recirculation of the small RNA fraction over the complementary NHS columun for 3 hours at 65°C ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Microbiology 2022Quote: ... Aldolase was detected using the above protocol with 1:10,000 mouse anti-aldolase antiserum (a kind gift from David Sullivan) and 1:2,500 sheep anti-mouse HRP-linked secondary antibodies (GE healthcare, NA931).
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bioRxiv - Immunology 2022Quote: ... The Fab fragments are then separated from non-digested IgG and Fc fragments by a 1 hr incubation with rProtein A Sepharose Fast Flow (GE Healthcare). Post incubation ...
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bioRxiv - Cell Biology 2022Quote: Figure 8 Supplement 1 was imaged using a DeltaVision Elite high-resolution imaging system equipped with a sCMOS 2048 × 2048 pixel camera (GE Healthcare). Z-stacks (0.2 μm step ...
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bioRxiv - Cell Biology 2022Quote: ... the codon-optimized sequence was synthesized (BioCat) and inserted into the BamHI and SalI sites of pGEX-6P-1 (GE Healthcare). For expression of ForB-N (aa 1-406 ...
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bioRxiv - Microbiology 2020Quote: ... put immediately on ice and 1 μL spotted on the positively charged nylon membrane (Amersham Hybon™-XL, GE Healthcare, France), which was exposed to UV at 254 nm for 10 min to fix DNA ...
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bioRxiv - Biochemistry 2021Quote: ... Immobilized metal ion affinity chromatography of the His-tagged Pdr5 was performed with a Zn2+ loaded 1 ml HiTrap Chelating column (GE Healthcare) and elution was carried out with a step gradient using low and high histidine buffers (50 mM Tris-HCl pH 7.8 ...
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bioRxiv - Cancer Biology 2020Quote: ... were cultured according to the ATCC guidelines in 4:1 mixture of Dulbecco’s medium and Medium 1999 supplemented with 10% Hyclone™ FBS (GE Healthcare) and 50μg/ml Gentimicin (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... 10 mM MgCl2 was added and periplasmic extracts were harvested by centrifugation and the supernatant was loaded onto a 1 ml HisTrap FF column (GE Healthcare). The nanobody was eluted via a linear gradient into 400 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellet was dissolved in 10 mL of buffer B and loaded onto a 1 mL HiTrap Heparin HP sepharose column (GE Healthcare) equilibrated with buffer B supplemented with 0.1 M NaCl ...
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bioRxiv - Biophysics 2020Quote: ... Cells were pelleted at 3500 g and the media containing secreted zRETECD was pooled and 1 ml of IgG sepharose resin (GE Healthcare) was added to 1 l of media and incubated at 4 °C for 18 hrs ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were then incubated for 1 h with a secondary antibody (goat anti-rabbit horseradish peroxidase-linked IgG [NA934V GE Healthcare] or sheep anti-mouse horseradish peroxidase-linked IgG [NA931V GE Healthcare] ...
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bioRxiv - Immunology 2021Quote: The scCl5 was purified using a two-steps protocol using first immobilized metal affinity chromatography (IMAC) to enrich for His tagged protein (1 ml HisTrap, GE Healthcare) followed by anion exchange (1 ml HiTrapQ ...
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bioRxiv - Biophysics 2021Quote: ... The mix was allowed to incubate for 1 hour over ice and loaded to a Superdex 200 Increase 10/300 GL size exclusion column (GE healthcare) pre-equilibrated in buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: Fragment screening of MsbA embedded in nanodiscs was performed with the Discovery-ZEN-LIBRARY 1 (Zenobia) in a Biacore 8K instrument (GE Healthcare) using an NTA Series S sensor chip (Cytiva ...
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bioRxiv - Biophysics 2020Quote: ... including an N term extension containing His9-HRV 3C protease site was cloned into the BamHI EcoRI sites in the MCS of pGEX-6P-1 vector (GE Healthcare). Site-directed mutagenesis was performed on the His9-SARS-CoV-2 Nucleocapsid pGEX vector to create the N protein constructs (SI Table XX and sequences were verified using Sanger sequencing ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Physiology 2022Quote: ... membranes were washed three times with 1X TBST and incubated with secondary antibodies in 5% milk in TBST for one hour at room temperature with the following concentration of secondary antibodies: donkey anti-rabbit IgG (1:5000, NA934, GE Healthcare) and goat anti-mouse IgG (1:5000 ...
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bioRxiv - Immunology 2022Quote: ... mononuclear cells were isolated by density gradient centrifugation of PBS-diluted buffy coat/blood (1:2) over Ficoll-Paque Plus (GE Healthcare). The PBMC layer was carefully removed and washed 3 times with PBS.
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bioRxiv - Cancer Biology 2022Quote: ... Blood was diluted 1:2 with 1× phosphate-buffered saline (PBS) and transferred to a 50 ml falcon tube with Ficoll-Paque PLUS (GE Healthcare) at a 1:2 ratio ...
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bioRxiv - Cell Biology 2022Quote: GST or GST-tagged β’-COP (residues 1-604) or Glo3 constructs (GAP domain, BoCCS region, GRM region) were immobilized on glutathione sepharose resin (GE Healthcare) for one hour on ice ...
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bioRxiv - Microbiology 2022Quote: ... cleavage was achieved after elution of GbpA from the column by adding 1 unit of PreScission protease enzyme and incubation overnight at 4°C per the instructions of the manufacturer (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: IVTT reaction mixtures (100 µl) were incubated at 4 °C for 1 h with a 50% slurry of PBS/Glutathione-Sepharose 4B (GE Healthcare) with gentle mixing ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to less than 1 mL and was injected onto a Superdex 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting supernatant (∼1 ml) was incubated with 20 µl of dimethyl pimelimidate (DMP) cross - linked IgG Sepharose 6 Fast Flow (GE healthcare) resin for 2 h with gentle rotation at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant protein in the soluble cell fraction was purified by immobilized metal affinity chromatography (IMAC) using a HiTrap Chelating HP 1-ml column (GE Healthcare) with an ÄKTA purifier FPLC system (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... Human CFP1 and truncation constructs were subcloned into the pGEX-6P-1 vector encoding an N-terminal glutathione S-transferase (GST) tag (GE Healthcare) prior to expression in Origami cells ...
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bioRxiv - Biophysics 2020Quote: ... Fixed cells were rinsed three times in warm PBS and incubated 30 min with a blocking solution containing 1% BSA (Bovine Serum Albumine, GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... Afterwards the immobilized papain resin was removed and the digested fraction was mixed with Protein A Sepharose (0.2 ml resin for 1 mg digested IgG, GE Healthcare, #17078001) for 1 hour at RT ...
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bioRxiv - Plant Biology 2019Quote: ... Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA) of biotinylated/streptavidin/horseradish peroxidase complex (GE Healthcare) before a final PBS/BSA rinse ...
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bioRxiv - Molecular Biology 2019Quote: ... was added to the protein solution to a final concentration of 1.3 μM and incubated for 15 minutes at room temperature in order to cleave off His10-SUMO tag and the protein solution was applied on HisTrap 1 HP (GE Healthcare) loaded with Zn2+ and pre-equilibrated with storage buffer ...
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bioRxiv - Biophysics 2020Quote: ... Bound proteins were eluted using a linear gradient (10 column volumes) from 0 to 1 M NaCl in buffer A using an Äkta Purifier (GE Healthcare). Elution fractions (1.6 ml ...