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Citations for GE Life Sciences :
2551 - 2600 of 5289 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... The protein-containing supernatant was then applied to PD-10 Sephadex desalting column (GE Healthcare) to remove access of peptide/dye ...
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bioRxiv - Biophysics 2022Quote: ... The complex was loaded on a Superose 6 10/300 column (GE healthcare, currently Cytiva) pre-equilibrated with 20 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2022Quote: Supernatants were collected and dialyzed to remove free biotin with PD-10 columns (GE Healthcare) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Nanodisc-embedded complexes were further purified using a Superose6 increase 10/300GL column (GE Healthcare) in a buffer containing 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... and a Superdex 200 Increase 10/300 column (28-9909-44, GE Healthcare, Uppsala, Sweden) equilibrated with 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP via gel filtration (Superdex 75 Increase 10/300; GE Healthcare Life Sciences). Protein peak fractions were combined and concentrated to 1 mM ...
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bioRxiv - Biophysics 2023Quote: ... The sample was applied to a Superdex 200 10/300 GL column (GE Healthcare, USA) pre-equilibrated with the reconstitution buffer ...
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bioRxiv - Biophysics 2022Quote: ... before purification on a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare) pre-equilibrated in buffer E ...
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bioRxiv - Biochemistry 2023Quote: ... samples were loaded onto a Superdex 200 (10/300) size-exclusion chromatography column (GE Healthcare) equilibrated with a buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... and loaded onto a Superdex S200 increase 10/300 GL size-exclusion column (GE Healthcare) equilibrated in wash buffer (with a reduced glycerol concentration of 2% when for cryo-EM studies) ...
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bioRxiv - Biophysics 2023Quote: ... Purified protein samples were resolved on a Superdex200 Increase 10/300 GL column (GE Healthcare) connected to a high-performance liquid chromatography (HPLC ...
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bioRxiv - Cell Biology 2024Quote: SEC-MALS was performed with a Superdex Increase S200 10/300 GL column (GE Healthcare) connected in-line with a sixteen-angle Dawn Heleos II light-scattering instrument and Optilab TrEX differential refractometer (Wyatt Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Samples were purified on a Superose 6 Increase 10/300 column (GE Healthcare Life Sciences) operating in TBS and leading SEC fractions were further concentrated to ∼1.1 mg/mL (JRCSF / Ab1456 sample ...
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bioRxiv - Biophysics 2024Quote: ... We used size exclusion chromatography (SEC) on a Superdex 200 10/300 column (GE Healthcare) to evaluate oligomerization state or collect fractions in either KBT or High-KBT (500 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... the protein elution was further purified by Superdex 200 Increase 10/300 GL (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... Final separation was achieved on a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES-KOH ...
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bioRxiv - Microbiology 2024Quote: ... 20-40 µg was added to a Superose 6 increase 10/300 column (GE Healthcare) and MALS spectra was recoded using inline DAWN-HELEOS light scattering and optilab T-rEX refractive index detectors (Wyatt) ...
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bioRxiv - Biophysics 2024Quote: ... further contaminants were separated by size-exclusion chromatography (Superdex 200 10/300 GL, GE Healthcare). Protein-containing fractions were dialyzed against Storage Buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... followed by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare) in 1X Tris- Buffered Saline (TBS ...
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bioRxiv - Immunology 2024Quote: ... followed by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare) in 1X Tris-Buffered Saline (TBS ...
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bioRxiv - Biochemistry 2024Quote: ... Biotinylated hURAT1EM was further purified by Superose 6 increase 10/300 GL column (GE Healthcare) running in a buffer containing 20 mM HEPES (pH=7.4) ...
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bioRxiv - Cancer Biology 2024Quote: ... the sample was separated using a Superdex 200 Increase 10/300 GL column (GE Healthcare). The peaks were analyzed by SDS-PAGE ...
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bioRxiv - Biophysics 2024Quote: ... The eluted samples were loaded onto a Superdex S200 10/300 GL column (GE Healthcare) pre-equilibrated with detergent buffer ...
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bioRxiv - Cell Biology 2024Quote: Size exclusion chromatography was performed on a Superose 12 10/300 GL column (GE Healthcare). The column was equilibrated with a buffer consisting of 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Further purification was done by SEC using a Superose 75 10/300 GL (GE Healthcare) column to remove excess nucleotides.
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bioRxiv - Biophysics 2021Quote: ... The cell lysates were centrifuged at 34,000 × g for 20 min at 4°C and the supernatant containing the His-tagged fusion protein was loaded onto a HisTrap column (AKTA-prime, GE Healthcare). The protein was eluted with a buffer containing 20–500 mM linear imidazole gradient ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was clarified by centrifugation (15000 rpm for 1 h at 4 °C) and incubated with glutathione Sepharose 4B (GE Healthcare) for 4 h at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The clarified cell lysates were incubated for 2 h on a spinning wheel at 4°C with 100µl of StrepTactin Sepharose High Performance beads (#28935599, GE Healthcare, USA). Beads were collected by centrifugation (1600 g for 5 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody-antigen complexes were recovered by incubating for 1 h at 4°C with Protein A-Sepharose CL-4B (GE Healthcare) and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysate made by sonication was centrifuged at 11,000 rpm for 30 min at 4◦C and the clear supernatant was purified through Ni-NTA column chromatography (GE Healthcare) following kit protocols ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated with GST or GST-tagged angulin-1 cytoplasmic region (409-575 aa or 409-570 aa) for 2-3 h at 4°C and further incubated with Glutathione Sepharose 4B beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Genetics 2021Quote: ... 2D gels were exposed for 4 hours in a phosphor screen cassette and the signal was detected with a Typhoon Trio phosphoimager (GE healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... and chromatin was released from the resin with 20 µl of ChIP buffer 1 supplemented with 100 µg/ml BSA and 4 µg HRV3C incubated (GE Healthcare) on ice for 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl) and GST-fused proteins were immobilized on Glutathione Sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-01) by incubating in a rotator at 4°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was cleared by centrifugation at 40,000 rpm in a Type 45 Ti rotor for 60 minutes at 4 °C and applied onto a pre-equilibrated HisTrap HP column (GE Healthcare) in CENP-E lysis buffer at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were then incubated with SuperSignal West Dura or ProSignal Pico Spray chemiluminescence substrate for 1-4 minutes at room temperature and imaged using an Amersham Imager 600 (GE Healthcare) or ChemiDoc (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and addition of 40 µl Protein A Sepharose resin (nProtein A Sepharose 4 Fast Flow, 17-5280, GE Healthcare) that had been blocked for 1 h in ChIP Blocking buffer (ChIP Low salt buffer containing 2 mg/ml BSA and 2% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10-30 μg of protein was electrophoresed on 4-12% (or 12% for OGG1ko blot) Bis-Tris gels (Thermo) before transferring to PDVF membranes (GE Healthcare). Membranes were blocked in 5% milk and blotted with primary and secondary HRP antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 12000 g at 4 °C for 1 h and loaded onto a HisTrap HP column (GE Healthcare) equilibrated with 98% buffer A/2% buffer B (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were purified at 4°C on the anti-FLAG resin at a flow rate of 20 ml/h with a P1 pump (GE Healthcare), and eluted by competition with a FLAG peptide solution at 200 μg/ml in 10 mM Hepes ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysates were quantified and 50 μg were resolved in 4-20% SDS-PAGE and transferred onto nitrocellulose membranes (GE healthcare). The membranes were then incubated with the primary antibodies ...
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bioRxiv - Biophysics 2021Quote: ... Total protein was collected and incubated with anti-p75NTR (Alomone, ANT-007, 1ug) overnight at 4 °C and then incubated with Sepharose protein-G beads (GE Healthcare). Samples were then prepared for immunoblotting as described below.
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bioRxiv - Biochemistry 2021Quote: ... MBP-PIPI was subjected to HRV-3C cleavage at 4°C overnight and was further purified in HiLoad 16/60 Superdex 200 preparatory grade size exclusion column (GE Healthcare) to separate fractions of polymerase proteins from cleaved MBP and protease ...
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bioRxiv - Neuroscience 2021Quote: ... cortices lysates were immunoprecipitated with anti-Bin1 or mouse IgG 16 h at 4 °C and then with 30 μl of protein G–Sepharose beads (GE Healthcare) for 3 h at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation at 18000*g for 45 min at 4 °C and the supernatant was applied to a Ni2+–Sepharose 6 Fast Flow (GE Healthcare) loaded gravity column ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 4 ml PBS and subject to density gradient centrifugation with Ficoll-Paque PLUS (17144002, GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Purification tags were then removed by incubation with 3C protease (10 μg/mg fusion protein) overnight at 4°C followed by passing through tandem Ni2+-NTA and MBP Trap HP columns (GE Healthcare). The flow-through was concentrated as appropriate and loaded on a Superdex 75 26/600 gel filtration column for final purification and buffer exchange into 20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked for 1 h at room temperature in TBS containing 4% Amersham ECL Prime Blocking Reagent (GE Healthcare) and 0.1% Tween 20 ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were then centrifuged at 20,000 g for 20 min at 4°C and the supernatant loaded to a GSTrap FF 1 mL column (GE Healthcare). Following a washing step with 10 mL GST-Buffer ...
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bioRxiv - Immunology 2021Quote: ... The affinity-purified protein was TEV-cleaved o/n at 4°C and subsequently subjected to gel filtration on an equilibrated Superdex 75 gel filtration column 16/600 (GE Healthcare). Monomer fractions were pooled and concentrated to 1 mg/ml ...