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Citations for GE Life Sciences :
2501 - 2550 of 4564 citations for Recombinant Human EGFR protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Protein expression was visualized with an Amersham ECL Western Blot System (GE Healthcare Life Sciences). β-Actin served as a loading control ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were subjected to anion exchange chromatography using the HiTrap™ Q column (GE Healthcare) using the ÄKTA™ start system (GE Healthcare) ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Protein bands were visualized using ECL Prime Western Blotting Detection Regent (GE Healthcare; Amersham, UK) and a light-capture cooled CCD camera system (ATTO Corp. ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins separated by 10 % Tris-glycine SDS–PAGE were transferred to nitrocellulose membranes (GE Healthcare) and processed for immunoblotting using the ECL Prime kit (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads followed by a HiLoad 16/60 Superdex 75 size exclusion chromatography.
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bioRxiv - Biochemistry 2024Quote: ... the proteins were purified with a Hiload 16/600 Superdex 200 pg column (GE Healthcare) preequilibrated with storage buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein expression levels were visualized with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Biochemistry 2024Quote: ... the protein elution was further purified by Superdex 200 Increase 10/300 GL (GE Healthcare).
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bioRxiv - Genetics 2024Quote: ... Proteins were detected by chemiluminescence using ECL SelectTM Western blotting Detection reagent (GE Healthcare, AmershamTM) and a Fujifilm LAS-3000 camera.
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 mm NC) at 90V for 90 min on ice in transfer buffer (48mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein bands were visualized and imaged by an ImageQuant LAS 500 scanner (GE Healthcare, USA). After transfer ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were purified from buffy coats by two gradient centrifugations: PBMCs were initially isolated with Ficoll-Paque (GE Healthcare, Pittsburgh, PA) and subsequently peripheral monocytes were isolated with Percoll (GE Healthcare ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were isolated from heparinised blood and tissue derived mononuclear cells were isolated from mechanically dissociated and filtered human tissue using Ficoll density gradient centrifugation (Ficoll PaquePlus; GE Healthcare, Amersham) or 33% Percoll (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: FH was isolated from human plasma using an affinity column made up of a monoclonal antibody against human FH (MRCOX23; MRC Immunochemistry Unit, University of Oxford) coupled to CNBr-activated Sepharose (GE Healthcare, UK) as described previously (21) ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2023Quote: Binding of mAbs to human FcRn was performed by measuring surface plasmon resonance (SPR) using a Biacore T200 instrument (GE Healthcare, UK), as described previously (42).
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bioRxiv - Microbiology 2019Quote: Human Custom ON-TARGET plus siKSRP or mimic miRs were resuspended in siRNA dilution buffer (Dharmacon, GE Healthcare, Chalfont-St-Giles, United Kingdom) to a 20 µM stock solution ...
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bioRxiv - Immunology 2022Quote: Cells were isolated from human blood using density gradient centrifugation agent Ficoll®-Paque Premium (17-5442-02, GE Healthcare, SIGMA, Darmstadt, Germany). Monocytes were plated at 3×105 cells per well in plastic 24 well plates ...
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bioRxiv - Immunology 2023Quote: ... freshly isolated mononuclear cells were separated from human blood by density centrifugation over Ficoll-Paque Plus (GE Healthcare, Chalfont St. Giles, United Kingdom). CD4+ T cells were subsequently purified by negative selection in an Automacs (Miltenyi Biotec ...
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bioRxiv - Biophysics 2021Quote: ... lysed protein was purified by cation exchange using a 1 mL HiTrap SP HP (GE Healthcare) in protein buffer (50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... The protein was purified from the lysate using Ni2+-NTA column of [KTA Pure (GE healthcare). For in vitro biotinylation of the polyprotein ...
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bioRxiv - Biophysics 2020Quote: ... the protein was loaded onto a HiLoadTM 26/10 Q Sepharose high performance column (GE Healthcare), and eluted at ∼350 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were purified by chromatography on a HiLoad 16/60 Superdex 200 column (GE Healthcare), in buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were blotted onto a nitrocellulose membrane (Amersham Protran 0.2 NC, GE Healthcare life sciences) for 1-2h at 300 mA ...
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bioRxiv - Cell Biology 2020Quote: ... The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences). The beads were washed with binding buffer at least four times ...
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bioRxiv - Cell Biology 2020Quote: ... Further purification of S-nitrosylated proteins were done using Thiopropyl Sepharose 6B bead (GE Healthcare# 170420001) in the presence of 50mM Na-ascorbate as a mild reducing agent ...
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bioRxiv - Immunology 2021Quote: ... Protein bands were visualised using Amersham™ Imager 600 (GE Healthcare Life Sciences, Malborough, MA, USA).
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bioRxiv - Microbiology 2021Quote: ... Protein-containing fractions were pooled for desalting through a 5 mL HiTrap Desalting column (GE Healthcare). Desalted protein was dialyzed into a storage buffer (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Developmental Biology 2020Quote: ... Immuno-detected proteins were visualized by chemiluminescent photographic film exposure (ECL kit; GE Life Sciences, RPN2232) and digitally scanned using a GS-800 calibrated densitometer (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was then purified by ion exchange chromatography (mono Q 10/100 GL, GE Healthcare), followed by SEC using a Superdex 200 increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was subjected to size-exclusion chromatography on a HighLoad 16/60 Superdex75 (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... protein-RNA complexes were immobilized on Protran nitrocellulose membrane (0.45 μm pore size, Whatman, GE Healthcare) and unbound RNA immobilized on Hybond Nylon membrane (Amersham ...
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bioRxiv - Molecular Biology 2020Quote: ... 1500 µg protein extract containing GST-Gal1p was loaded on a GST SpinTrap column (GE Healthcare) and incubated for 1.5 hour at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The protein was further purified by anion-exchange chromatography on a Mono-Q column (GE healthcare). Expression and purification of RT600 was carried out in a similar way as with RT139A except that the 6x-His tag on p51 N-terminus was not cleaved ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... A suspension of Optison microbubbles (Perflutren Protein-Type A Microspheres Injectable Suspension, USP, GE Healthcare, USA) was infused intravenously (15 μl/minute ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: ... Eluted protein was concentrated and subjected to a HiLoad 16/600 Superdex 75 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... protein supernatant was bound to a phenyl sepharose column (High Load FF 16/10, GE Healthcare). The column was washed with buffer C and protein eluted with 30% ethanol in water ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was fractionated on a HiLoad 16/600 Superdex 200 gel filtration column (GE Healthcare), eluting with SEC buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein A Sepharose 4 Fast Flow and Con A Sepharose 4B were purchased from GE Healthcare.
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bioRxiv - Molecular Biology 2019Quote: ... the reaction was added to 25 µL (50 µL 50% slurry) Protein G beads (GE Healthcare) pre-coupled with antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... the protein was further purified through HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare) and eluted in buffer containing 20 mM Tris-Cl (pH 7.5) ...
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bioRxiv - Immunology 2019Quote: ... The expected protein bands were detected using Image Quant LAS 4000 (GE Healthcare Life Sciences, USA). The relative abundance of the target protein (normalized to β-actin ...