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Citations for GE Life Sciences :
2451 - 2500 of 2764 citations for Toll Like Receptor 5 TLR5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Anti-rabbit IgG HRP-linked secondary antibody and 0.45-μm polyvinylidene difluoride (PVDF) membrane were obtained from GE healthcare. Specific-Pathogen-Free (SPF ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with horseradish peroxidase-conjugated sheep anti-mouse IgG polyclonal antibody (GE Healthcare, Chicago, IL, USA). Antigen-antibody interactions were detected using o-phenylenediamine solution as the substrate (Nacalai Tesque) ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated with a rabbit polyclonal anti-HDA15 antibody and protein G Mag Sepharose beads (GE Healthcare) overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... HRP-conjugated secondary antibodies were detected with WesternBright ECL chemiluminescent HRP substrate using a ImageQuant imaging unit (GE Healthcare). Band intensities for reference protein standards and neuropil extracts were calculated in ImageJ ...
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bioRxiv - Cell Biology 2020Quote: ... Signals from HRP-conjugated secondary antibodies were visualized by chemiluminescence (Amersham ECL start Western Blotting Detection Reagent, GE Healthcare) and recorded using the ImageQuant LAS 4000 Imaging System (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... PVDF membranes were washed and revealed using horseradish peroxidase (HRP)-conjugated secondary antibodies and ECL detection reagent (GE Healthcare). The blots were imaged on a Geliance gel imaging systems (PerkinElmer ...
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bioRxiv - Neuroscience 2021Quote: ... Protein-antibody complexes were detected on X-ray films following addition of Enhanced Chemiluminescence (ECL) substrate (GE Healthcare, UK). The relative density of the pCREB and CREB bands was quantitated using ImageJ software (NIH ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were detected with horseradish peroxidase–conjugated anti-mouse or anti-rabbit antibodies (GE Healthcare Life Sciences, Marlborough, MA).
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bioRxiv - Microbiology 2022Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
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bioRxiv - Microbiology 2022Quote: ... IgG1 and IgG2 antibodies were isolated from cell supernatants using a HiTrap Protein A High Performance column (GE Healthcare), whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibody staining using either sheep α-mouse or donkey α-rabbit (NA931V, NA934V, GE HealthCare, Chicago, IL, USA) was performed for 1 hour on a tube roller at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... For all primary mouse antibodies an anti-mouse-HRP in a dilution of 1:2000 was used (GE Healthcare). All blots were blocked with TBS-T 5% Milk and washed 3x for 15 minutes with TBS-T after each antibody incubation ...
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bioRxiv - Microbiology 2022Quote: ... The antibodies were purified on an AKTA FPLC using GE Superdex 200 increase 10/300 GL column (GE HealthCare) in 1× PBS.
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bioRxiv - Microbiology 2022Quote: ... antibodies diluted 1:500 in 1 U/mL DNAse I in Tris Buffered Saline containing 1% BSA (GE Healthcare). Three 5-minute washes with block (1% BSA with 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2023Quote: ... Appropriate antibodies were incubated with clarified lysate for overnight at 4°C and protein G sepharose beads (GE Healthcare) were incubated for 2h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... HRP-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were purchased from GE Healthcare (Chicago, IL, USA). Doxycycline ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was incubated with secondary antibodies and visualized with the ECL Detection System (GE Healthcare, Little Chalfont, UK).
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... a CM5 sensor chip was covalently modified by an anti-GST polyclonal goat antibody (GST capture Kit, GE Healthcare). The surface of flow cell 1 and 2 was activated for 60 s with 50 mM NaOH (10 μL/min) ...
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bioRxiv - Cell Biology 2024Quote: ... Visualization of the antibody binding sites was achieved using Amersham ECL Prime Western Blotting (GE Healthcare UK Ltd, RPN2232) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2024Quote: ... at a 1:10,000 dilution or a donkey α-rabbit antibody conjugated to HRP (GE Life sciences, Marlborough MA) at a 1:10,000 dilution ...
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bioRxiv - Immunology 2022Quote: ... Immunoreactive bands were detected using HRP-conjugated secondary antibodies and revealed with ECL substrate (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biochemistry 2022Quote: ... Bound secondary antibodies detected by chemiluminescence using the ECL kit according to the manufacturer’s instructions (GE Healthcare, Uppsala, Sweden). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies and associated DNA were then isolated using Gammabind G Sepharose beads (GE Healthcare Bio, catalog # 17-0885-01). Crosslinks were reversed by incubating samples and input at 65°C for at least 6 hours ...
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bioRxiv - Immunology 2022Quote: The IgG antibody or Fab binding properties were analyzed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... Detection of HRP-linked antibodies was performed using chemiluminescence detection kits (ECL™ or ECL Plus™, GE Healthcare) according to the manufacturer’s instructions and visualized using ChemiDoc™ Touch Imaging System (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies (anti-rabbit IgG, anti-mouse IgG or protein-A) coupled with horseradish peroxidase were from GE Healthcare, an anti-goat IgG secondary antibody was from Santa Cruz.
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bioRxiv - Cell Biology 2023Quote: ... IgG and target specific antibodies as well as protein G sepharose 4 Fast Flow beads (GE Healthcare Life Sciences) were used ...
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bioRxiv - Cancer Biology 2022Quote: ... using HRP-conjugated secondary anti-mouse antibody (2.5:10 000) and Amersham ECL prime detection reagent (GE healthcare lifescience).
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bioRxiv - Immunology 2022Quote: Antibody Fab fragments were purified further via size-exclusion chromatography using an S200 increase 10/30 column (GE Healthcare) equilibrated with 25 mM Tris (pH 8.0) ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were rinsed in PBST and bound antibodies detected with ECL (GE Healthcare Life Sciences, Chicago, IL, USA).
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bioRxiv - Cell Biology 2023Quote: ... The antibody was affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
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bioRxiv - Cancer Biology 2023Quote: ... The unconjugated DFO was removed from the antibody-DFO conjugates using a size exclusion column (PD-10; GE Healthcare) and the antibody solutions were concentrated using amicon filters with a 50 kDa cutoff filter ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed with 3X with TBST buffer and incubated with appropriate HRP-conjugated secondary antibodies (GE Healthcare) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 1 h at room temperature with HRP-conjugated rabbit or mouse secondary antibodies (1:50,000, GE Healthcare). Enhanced chemiluminescence (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... The antibodies were affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
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bioRxiv - Cancer Biology 2023Quote: ... A875 cell extracts were incubated for 16 hr at 4°C on a rotating wheel with 0.8μg of anti-p75NTR antibody (ANT-007, Alomone) and then incubated with Sepharose protein-G beads (GE Healthcare). The beads were collected by brief centrifugation (2 min ...
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bioRxiv - Cell Biology 2023Quote: ... Detection was carried out using peroxidase-conjugated secondary antibodies and the ECL Plus Reagent (GE Healthcare, Chicago, IL, USA) or SuperSignal West Femto (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
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bioRxiv - Microbiology 2024Quote: ... was used in combination with a secondary anti-mouse antibody conjugated to horseradish peroxidase (GE Healthcare NXA931, 1:5000). Primary rabbit anti-mCherry antibody (BioVision 5993 1:2000 ...
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bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...
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bioRxiv - Cell Biology 2020Quote: ... Cell Signalling) and horseradish peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (respectively NA931V and NA934V. 1:10.000. GE Healthcare). Mineral dissolution activity of Oc was measured as described (Maurin et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection of HRP-conjugated secondary antibody was carried out using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare). Where representative blots are shown ...
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody was anti-mouse HRP (horseradish peroxidase)-linked (NA931, Amersham ECL Mouse IgG, HRP, GE Healthcare Life Science) diluted 1:10000 in 5% dry milk in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... and HRP-labelled anti-mouse secondary antibody before bands being detected using an enhanced chemiluminescence kit (GE Healthcare, Buckingham, UK). β-actin (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were washed three times in TBST before incubation with HRP-conjugated secondary antibodies for 1hr at room temperature in 2% milk TBST (1/10,000, GE Healthcare).
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bioRxiv - Developmental Biology 2019Quote: ... the membrane was incubated with an appropriate secondary antibody (anti-rabbit or anti-mouse IgG HRP-conjugated Ab (1:10,000 in 3% skim milk in TBS, GE Healthcare), mouse TrueBlot ULTRA ...
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bioRxiv - Genetics 2019Quote: ... we used the anti-rabbit HRP-conjugated antibody (1/5000 dilutions) with the ECL Western Blotting Detection System (GE Healthcare) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... the membranes were incubated with 10,000-fold diluted peroxidase-linked secondary antibody (NA931V for FLAG-BRCA2 and NA934V for beta-Actin; GE Healthcare) at room temperature for 4 hours ...