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Citations for GE Life Sciences :
2451 - 2500 of 4264 citations for Recombinant Human CTRL Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The protein sample (50 μL) was loaded into a 10/300 Superdex® 200 GL (GE Healthcare) column connected to the MALS detection unit for SEC ...
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bioRxiv - Biochemistry 2020Quote: ... Concentrated protein was then applied to a size exclusion column (HiLoad 16/60 Superdex 200, GE Healthcare) pre-equilibrated with Buffer GF (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The protein complex eluted as a monodisperse peak from a Superdex 200 gel filtration column (GE Healthcare) in 20 mM HEPES/NaOH pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were further purified using Superdex 75 16/60 or Superdex 200 16/60 (GE Healthcare) gel filtration chromatography and stored in their respective running buffers ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated and run over a HiLoad 16/600 superdex 75 gel filtration column (GE Healthcare) using an AKTA pure (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The cells were collected and lysed and protein was purified with His-Trap chelating column (GE Healthcare). The purified recombinant proteins were analysed by Coomassie-stained SDS–polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... 0.3 M NaCl using a Fast Protein Liquid Chromatography (FPLC) system (ÄKTA Purifier, GE Healthcare Life Sciences). Prior to loading ...
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bioRxiv - Neuroscience 2019Quote: ... Eluted protein was concentrated and further purified on a Superose 6 10/300 Increase column (GE Healthcare) with 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... The proteins were further purified using 16/600 Superdex 75 pg size-exclusion chromatography columns (GE Healthcare) equilibrated in protein buffer (20mM Tris pH 7.8 ...
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bioRxiv - Neuroscience 2020Quote: ... Immune complexes were isolated by the addition of 20 μL of protein G– Sepharose beads (GE Healthcare) followed by incubation for 2 hr at 4°C ...
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bioRxiv - Biophysics 2021Quote: Purified protein or cell lysates were injected onto a Superose 6 increase 5/150 column (GE Healthcare), running in a buffer containing 20 mM Tris (pH 7.5) ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted protein was further purified by size exclusion chromatography using a Superdex 200 prepgrade column (GE Healthcare) in 50 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... The reconstituted protein was further purified on the Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated in a buffer comprising 20 mM Bis-Tris pH 6.8 ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing protein of interest were collected and desalted using PD-10 midi desalting columns (GE Healthcare). Protein of interest was eluted with the storage buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibodies and dilutions used were ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibody and dilution used was rat anti-HA-HRP 3F10 (Roche Applied Science) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated using SDS-PAGE and blotted onto Amersham Protran 0.45 µm nitrocellulose membrane (GE Healthcare). The primary antibodies and dilutions were ...
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bioRxiv - Cell Biology 2021Quote: ... Labeled proteins were purified by final buffer exchange using size exclusion chromatography (Superdex 200 column, GE Healthcare) in the appropriate buffer (i.e ...
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bioRxiv - Microbiology 2020Quote: ... The concentrated DnaA protein was subjected to size exclusion chromatography on an Äkta pure instrument (GE Healthcare). 200 μL of concentrated DnaA-6xHis was applied to a Superdex 200 Increase 10/30 column (GE Healthcare ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were detected by Enhanced Chemiluminescence (ECL) using ECL Western Blotting reagents (Amersham, GE Healthcare, UK) or SuperSignal West Dura Extended Duration Substrate (Pierce ...
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... denatured proteins were then separated via SDS-PAGE and transferred to a PVDF membrane (GE Healthcare, Amersham). Blots were preincubated in a blocking solution of 5% BSA in 0.2% TBST (0.1 M Tris base ...
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bioRxiv - Plant Biology 2020Quote: ... the purified RCA protein was immobilized on a carboxy-methylated dextran sensor chip (CM5) chip (GE Healthcare) using an amine coupling method kit (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin samples derived from 1.0×106 cells were precleared with protein G sepharose (GE Healthcare, Chicago, IL) and then 1-5 μg of each antibody was added to form immune complex ...
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bioRxiv - Microbiology 2020Quote: ... supernatant containing VSG protein was loaded onto an anion-exchange column (Q-Sepharose Fast-Flow, GE Healthcare), which had been equilibrated with 10 mM phosphate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified by immobilized metal affinity chromatography using the His Trap Kit from GE Healthcare Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Microbiology 2020Quote: ... FLAG epitope-tagged protein levels were visualized using the Amersham ECL western blotting detection reagent (GE Healthcare). Thereafter ...
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bioRxiv - Microbiology 2019Quote: ... Typically 100 µg of protein samples were injected into Superdex 200 10/300 increase column (GE Healthcare) by a Shimadzu autosampler coupled HPLC machine ...
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bioRxiv - Cell Biology 2021Quote: ... between 600-900 μg of each CLASP1 protein was injected into a Superose 6 column (GE Healthcare) in a buffer containing 50 mM phosphate pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... The next morning the cleaved protein was loaded on a 5 ml HiTrap HeparinHP column (GE Healthcare). The protein was eluted over a gradient from 100 mM to 2M KCl ...
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bioRxiv - Biophysics 2020Quote: ... the protein solution was fractionated using a DEAE Sepharose Fast Flow anion exchange chromatography resin (GE healthcare) eluting with a gradient from 0 to 1 M NaCl and further fractionated by size-exclusion chromatography using a HiPrep™ 26/60 Sephacryl® S-500 HR column ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were then electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... The immuno-complexes were collected with 50 μl of protein A sepharose (17-0780-01, GE Healthcare) at 4 °C for two hours with rotation ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoreactive protein bands were visualized via enhanced chemiluminescence and scanned with a LAS-4000 system (GE Healthcare).
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bioRxiv - Evolutionary Biology 2022Quote: ... The protein sample was further purified using a Superdex200 26/600 size-exclusion chromatography column (GE Healthcare), and fractions were collected and concentrated to 1.8 mg/ml in 10 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated and further purified on a Superdex 75 16/600 GL column (GE Healthcare), in a size exclusion buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: Equal amounts of protein were resolved on 10% SDS-PAGE and blotted onto nitrocellulose membranes (GE Healthcare). Immunoblots were blocked with 5% fat-free milk in PBS containing 0.1% Tween-20 for 1h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... ActRIIB-Fc was isolated using affinity chromatography with Mab Select Sure Protein A (GE healthcare - Waukesha, WI), followed by dialysis into 10mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The tagless protein was further purified by anion exchange chromatography (Mono Q 10/100 GL, GE Healthcare) in 25 mM potassium phosphate (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: Samples were precleared by adding 60 μl of 20% Protein A Sepharose™ CL-4B (GE Healthcare) in IP Buffer (15 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... the buffer exchanged protein was concentrated and injected on a SD200 10/300 increase column (GE Healthcare) equilibrated with 25 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Anti-TNFα IgG1 was purified on Protein A HiTrapHP columns (GE Healthcare Life Sciences, Little Chalfront, UK) using Akta-prime plus (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... partition coefficients of standard proteins from the high-molecular-weight gel filtration calibration kit (GE Healthcare #28403842) were plotted against Log(MW ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified using size exclusion chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) to remove nonspecific proteins ...
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bioRxiv - Biochemistry 2022Quote: ... Collected fractions containing membrane proteins were loaded onto a Superose 6 increase 10/300 column (GE Healthcare) and eluted with 30 mL of NCMN Buffer E ...
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bioRxiv - Biophysics 2022Quote: ... the modified proteins were purified and separated from unbound dye through a gel filtration column (GE Healthcare illustra NAP Columns from Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified by size exclusion chromatography using Superdex 200 16/60 column (GE Healthcare) equilibrated in buffer containing 25mM Tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2022Quote: Samples were separated on SDS-polyacrylamide gel and proteins were transferred onto a nitrocellulose membrane (GE healthcare) using a wet transfer system (BioRad) ...
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bioRxiv - Immunology 2020Quote: ... the supernatants with the secretion of antibodies were collected and captured by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and dialyzed into phosphate-buffered saline (PBS) ...