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Citations for GE Life Sciences :
201 - 250 of 1208 citations for Recombinant Human SGK3 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: GST-MLLE protein was applied to Series S sensor chip CM5 bound with anti-GST antibodies (GE Healthcare BR100223), in Biacore T100 ...
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bioRxiv - Neuroscience 2020Quote: ... GST-hMeCP2 or GST-OGT fusion proteins were immobilized on glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) and washed three times with GST binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... a CM5 sensor chip was covalently modified by an anti-GST polyclonal goat antibody (GST capture Kit, GE Healthcare). The surface of flow cell 1 and 2 was activated for 60 s with 50 mM NaOH (10 μL/min) ...
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bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
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bioRxiv - Cancer Biology 2023Quote: ... Each GST-fusion protein was released from GST adsorbed to glutathione-sepharose by PreScission protease (GE Healthcare, 27-0843-01). The concentrations of purified proteins were determined by Bradford analysis (Bio-Rad 5000006) ...
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bioRxiv - Cell Biology 2020Quote: XMAP215-GFP-7xHis was purified using His-affinity (His-Trap, GE Healthcare) by binding in buffer (50mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... and the His-tag of Rab8a (anti-His-Antibody, GE Healthcare, mouse). Both were diluted (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... GST-hEBP50 in pGEX-6P-2 (GE Healthcare): GST-hEBP50/WT (AA95-168) ...
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bioRxiv - Neuroscience 2021Quote: ... GST (goat, GE Healthcare, 27457701V, WB 1:5000), MYC (mouse ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were bound to GST resin (GE Healthcare) and eluted in buffer A supplemented with 10 mM glutathione (pH-calibrated) ...
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bioRxiv - Plant Biology 2022Quote: ... and GST-fusions by glutathione-resin (GE Healthcare), each according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... 2 µl of either anti-GST (GE Healthcare), anti-HA (Sigma H6908) ...
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bioRxiv - Biochemistry 2020Quote: ... MLL1-PHD3 and 6x-His-FL-Cyp33) or Superdex G200 column (His-MBP-FL-Cyp33 and His-SUMO-FL-Cyp33) (both columns from GE Healthcare). After elution ...
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bioRxiv - Cell Biology 2021Quote: ... The GST and GST fusion protein extracts were mixed with glutathione Sepharose 4B beads (71024800-GE; GE Healthcare, Chicago, IL, USA) overnight at 4 °C and the mixtures were then washed three times with ice-cold PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was incubated with GST-CDK2 and then purified by affinity chromatography using Sepharose 4B GST resin beads (GE Healthcare) and subsequently eluted in 20 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: 10 μM purified WIPI2d was mixed with 20 μM of GST or GST-ATG16L1(207-230) and 10 μL Glutathione Sepharose 4B (GE Healthcare). The final buffer was 25mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... 80 picomoles of GST or GST-Tvactin immobilized on 20 μl of glutathione conjugated-sepharose beads 4B (GE healthcare, Illinois, USA) was incubated with 80 picomoles of His-TvFACPα or derived mutants in 1 ml GST binding buffer (PBS contains 0.2% Triton X-100 and 1 mM EDTA ...
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bioRxiv - Cell Biology 2019Quote: ... Extracts of GST or GST-fusion proteins were incubated for 0.5-1h with glutathione-coated sepharose beads (GE Healthcare, Freiburg, Germany) equilibrated in PBS (Boi1-Bni1 ...
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bioRxiv - Microbiology 2021Quote: Binding of C1 and its sub-components to GST-ErpB and GST-ErpQ was performed at 25°C using a Biacore T200 (GE Healthcare) as previously described (Garcia 2016) ...
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bioRxiv - Biochemistry 2020Quote: ... purified fluorescent ULK1 complex (FIP200-MBP/EGFP-ATG13/EGFP-ATG101/ULK1) was mixed with purified GST-NDP52 or GST-4xUb and 10 μL Glutathione Sepharose 4B (GE Healthcare). The final buffer was 20 mM HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... CpxA31-163-GST was purified at 4°C using a protocol modified from the GST Gene Fusion System Handbook (GE Healthcare). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... 500μl of dialyzed GST-Bsd or GST was bound to 50μl of PBS washed glutathione sepharose beads (GE Healthcare, 17-0756-01) and incubated at 4°C for 30min ...
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bioRxiv - Cell Biology 2021Quote: ... bacterial-expressed GST and GST-α-Taxilin-M (expessed in Escherichia coli strain BL21) were bound to glutathione-Sepharose 4B beads (GE Healthcare) and incubated with bacterial-expressed MBP-γ-Taxilin-M or 293T cell expressed and purified 3×FLAG-CEP170 at 4 °C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... and GST were purified on Glutathione Sepharose (GE Healthcare) by batch purification ...
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bioRxiv - Biochemistry 2020Quote: ... and treated overnight with GST-PreScission Protease (GE Healthcare). Eluted proteins were further purified over a Superdex 75 16/600 pd column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... goat anti-GST HRP-conjugated antibody (RRID:AB_771429; GE Healthcare). Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was added GST agarose beads (GE Healthcare) and rotated at 8 °C for 1.5 hr ...
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bioRxiv - Microbiology 2021Quote: ... plates were incubated with goat anti-GST (GE Healthcare) for an hour and GST binding was detected kinetically using an alkaline-phosphatase-linked rabbit anti-goat IgG secondary antibody (diluted 1:2000 in 0.1% BSA/PBS) ...
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bioRxiv - Biochemistry 2021Quote: ... cleared lysate was applied on GST-Trap (GE Healthcare) before extensive washing with ly-sis buffer ...
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bioRxiv - Genetics 2023Quote: ... Glutathione SepharoseTM 4B (for GST fusions; GE Healthcare, 17075601) or amylose resin (for MBP fusions ...
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bioRxiv - Plant Biology 2023Quote: ... pGEX-2T (GE Healthcare, N-terminal fusion with GST) was used for CPK protein production in bacteria for previously reported constructs72 (CPK2/3/5/11) ...
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bioRxiv - Biochemistry 2024Quote: ... loaded onto 5 ml GST-trap column (GE Healthcare), equilibrated in buffer A ...
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bioRxiv - Molecular Biology 2022Quote: ... The CobB protein was separated from His tag on His-Trap column (GE Healthcare), concentrated at 4 °C to 6 mg ml-1 ...
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bioRxiv - Molecular Biology 2020Quote: ... Hi-Trap Q (GE Healthcare), and Superdex 200 columns (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare, Chicago, IL) at 4 °C for 2 h ...
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bioRxiv - Plant Biology 2020Quote: ... and Anti-GST-HRP conjugate (RPN1236; GE Healthcare Bio-Sciences) were used to detect MBP-SPA1 and GST or GST-PIF4 ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-KCTD19 was purified using Glutathione Sepharose 4B (GE Healthcare). The purified KCTD19 protein with a complete adjuvant was injected into female rats ...
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bioRxiv - Molecular Biology 2019Quote: ... the GST sequence from the pGEX-6P plasmid (GE Healthcare) was amplified using primers 5’-GGCAGCAGCCATCATCATCATCATC ACAGCAGCGGCCTGATGTCCCCTATACTAGGTTATTGG-3’ and 5’-TCTTCTTTTGGAACCCA TCATATGGCTGCCGCGCGGCACGGGCCCCTGGAACAGA-3’ ...
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bioRxiv - Cell Biology 2022Quote: Goat GST antibody (27457701V, GE Healthcare, used at 1:1,000), NeutrAvidin-DyLight 800 (22853 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were pooled onto GST affinity resin (GE Healthcare), incubated for 2 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The GST tag was cleaved with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Biophysics 2022Quote: ... the Glutathione Sepharose 4B affinity beads (GST beads) (GE Healthcare) were washed three times with buffer 25 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... which was applied on a GST-sepharose column (GE Healthcare). After washing with sonication buffer ...
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bioRxiv - Developmental Biology 2020Quote: GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ...
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bioRxiv - Biochemistry 2021Quote: ... the GST eluent was incubated with Benzamidine-Sepharose (GE Healthcare) for 30 min at 4 °C.
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bioRxiv - Biochemistry 2021Quote: ... GST-Sds3 CTD was purified with glutathione sepharose (GE Healthcare) and eluted using 25 mM glutathione ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-TRIM41 was purified using Glutathione Sepharose 4B (GE Healthcare). The GST tag was removal by PreScission protease (27084301 ...
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bioRxiv - Molecular Biology 2023Quote: ... The GST tag was cleaved by PreScission Protease (GE Healthcare) treatment over 12 hrs at 4°C with the progress of reaction monitored by SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... GST-Rheb was cloned into pGEX (GE Healthcare Life Sciences). Silencer Select siRNAs of Rheb (s12019 ...