Labshake search
Citations for GE Life Sciences :
201 - 250 of 4228 citations for Recombinant Human MAG Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
-
bioRxiv - Genetics 2021Quote: ... and RNA was extracted from cells using a magnetic bead-based protocol using Sera-Mag SpeedBeadsTM (GE Healthcare). Two libraries were prepared per clone using SMARTseqv4 kit (Clonetech) ...
-
bioRxiv - Molecular Biology 2020Quote: ... RAS:Affimer immunoprecipitation was as for RAS:RAF immunoprecipitation except His Mag Sepharose™ Ni2+-NTA beads (GE healthcare®) were used and incubated with 20 µg of Affimer K3 for 1 h with agitation ...
-
bioRxiv - Genomics 2020Quote: ... RNA was extracted from cells using a magnetic bead-based protocol using Sera-Mag SpeedBeads™ (GE Healthcare). Isolated RNA was DNase-treated with RQ1 DNase (Promega) ...
-
bioRxiv - Microbiology 2020Quote: Solid-phase reversible immobilisation (SPRI) on carboxylated paramagnetic beads (Sera-Mag™ Magnetic SpeedBeads™, from GE Healthcare) were prepared for RNA binding as described (https://openwetware.org/wiki/SPRI_bead_mix) ...
-
bioRxiv - Microbiology 2020Quote: ... Supernatant was incubated with His Mag Sepharose beads (equilibrated with lysis buffer according to manufacturer’s instructions; GE Healthcare) for 30 min at 4 °C rotating end-over-end ...
-
bioRxiv - Biochemistry 2020Quote: ... Sera-Mag Oligo(dT)-coated magnetic particles and Hybond-N+ membranes were obtained from GE Healthcare (Chicago, IL). GENEzol™ reagent was purchased from Geneaid Biotech (New Taipei ...
-
bioRxiv - Microbiology 2021Quote: ... The supernatant containing cDNA-linker-VHH complex was purified using His Mag Sepharose Ni beads (GE Healthcare, USA). The supernatant was added to 20 μL of the beads and incubated at 25°C for 30 min ...
-
bioRxiv - Systems Biology 2023Quote: To prepare activated magnetic beads 6.25mg of Sera-Mag SpeedBead hydrophilic carboxylate-modified paramagnetic particles (GE Life Sciences) were washed twice in MES buffer (500 mM 2-(N-morpholino)ethanesulfonic acid ...
-
bioRxiv - Immunology 2021Quote: ... sterilized by filtration with a 0.4 μm filter and recombinant proteins were purified with HisTrap TALON FF crude or HisTrap Excel columns (GE Healthcare Life Sciences).
-
bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrapTM (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
-
bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
-
bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
-
bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
-
bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
-
bioRxiv - Genomics 2021Quote: ... and DNA was bead purified with 1.6x homebrew solid phase reversible immobilization (SPRI) beads (1mL Sera-Mag SpeedBeads (GE Healthcare), 9g PEG 8000 (Sigma) ...
-
bioRxiv - Biochemistry 2019Quote: ... and hydrophobic Sera-Mag SpeedBeads carboxylate-modified magnetic beads (conc. 10µg/µL; GE Life Sciences, cat. no. 6515-2105-050250) were combined in a 1.5ml flex tube (Eppendorf ...
-
Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... Sample clean-up and library preparation was carried out using Sera-Mag Speedbeads hydrophobic carboxyl magnetic beads (GE Healthcare, 65152105050250). Following bead cleanup ...
-
bioRxiv - Microbiology 2020Quote: ... reverse-crosslinked samples were combined and the DNA was purified using solid phase reverse immobilization (SPRI) Sera-Mag SpeedBeads (1.6:1 ratio, GE Healthcare). The final library was eluted with 22 μL ddH2O.
-
bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
-
bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
-
bioRxiv - Biochemistry 2020Quote: ... and Single-Pot Solid-Phase enhanced Sample Preparation (SP3) using paramagnetic beads (GEHE45152105050250 Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophilic, GE Healthcare) and GEHE65152105050250 Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophobic ...
-
bioRxiv - Biochemistry 2021Quote: ... A 1:1 combination mix of two of magnetic carboxylate beads was used (Sera-Mag Speed beads, #45152105050250, #65152105050250, GE Healthcare). Beads were prepared fresh by rinsing with water three times prior to use at a stock concentration of 20 μg/μL ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was cleaned up using solid phase reversible immobilization (SPRI) beads (Sera Mag™, GE Healthcare Life Sciences, Chicago, IL, USA) in a homemade bead binding buffer containing 22% polyethylene glycol (PEG) ...
-
bioRxiv - Cell Biology 2022Quote: ... isoform 1 with N-terminal MRGS(H)8 and C-terminal Avi tag (Hismyosin VI (aa 992-1031)Avi) was immobilized on either MyOne T1 streptavidin-coated beads (Pierce) or Sera-Mag neutravidin-coated beads (GE Healthcare). The use of the type of beads was alternated during selection rounds ...
-
bioRxiv - Systems Biology 2023Quote: ... 50 µL of a 20 µg/µL SP3 bead stock (Sera-Mag SpeedBead carboxylate-modified magnetic particles; GE Healthcare Life Sciences) and 500 µL acetonitrile (ACN ...
-
bioRxiv - Immunology 2023Quote: ... Total RNA was extracted and from cell monolayers using a magnetic bead-based protocol using Sera-Mag SpeedBeads™ (GE Healthcare). Isolated RNA was DNase-treated with RQ1 DNase (Promega) ...
-
bioRxiv - Genomics 2023Quote: ... The phage DNA is then purified using 1.6x volume of homebrewed solid phase reversible immobilization (SPRI beads) (1mL Sera-Mag SpeedBeads (GE Healthcare, GE1715210401150), 9g PEG 8000 (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... The magnetic bead mixes were prepared by combining equal parts of Sera-Mag Carboxyl hydrophilic and hydrophobic particles (09-981-121 and 09-981-123, GE Healthcare). The bead mix was washed three times with MS-grade water and resuspended in a working concentration of 10 µg/µl ...
-
bioRxiv - Systems Biology 2024Quote: ... SP3 magnetic beads were prepared as follows: per 10 samples 20 µL of each Sera-Mag Speed Beads A and B (GE Healthcare) were mixed 1:1 ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 µL of a 1:1 mix of two types of commercially available carboxylate beads (Sera-Mag Speed beads, 45152105050250, 65152105050250, GE Healthcare), and 60 µL ethanol ...
-
bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
-
bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
-
bioRxiv - Microbiology 2022Quote: ... Triplicate PCR reactions were then pooled and purified with magnetic beads (Sera-Mag™ Select, GE Healthcare, Chicago, United States of America), and 2 μl of the purified products were used at a template for a second stage PCR ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... After the restriction enzyme digestion step and the adapter ligation, large cutoff bead-cleanups (0.6:1, bead:library ratio) with homemade magnetic beads (Sera-Mag SpeedBeads™, #65152105050450, GE Healthcare Life Sciences) in PEG/NaCL buffer89 were used to clean the samples from the buffers and remove large fragments above ∼600 bp length ...
-
bioRxiv - Genomics 2020Quote: ... mRNA was extracted from approximately 5 μg of the total RNA samples using Sera-Mag oligo-dT beads (GE Healthcare Life Sciences). The mRNA was fragmented and used to prepare sequencing libraries according to the Illumina TruSeq Stranded mRNA sample preparation kit ...
-
bioRxiv - Systems Biology 2022Quote: ... was combined with 250 μg carboxylated paramagnetic beads (1:1 mix of hydrophiclic and hydrophobic Sera-Mag SpeedBead Carboxylate-Modified, GE Life Sciences) and 750 μl ethanol ...
-
bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
-
bioRxiv - Microbiology 2019Quote: ... Samples were incubated at room temperature for 1 h and DNA was extracted with magnetic Sera-Mag Speedbeads (GE Healthcare Life Sciences, UK) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were mixed with 10μl of 10μg/μl solution of Sera-Mag SpeedBeadsTM hydrophilic and hydrophobic magnetics beads (GE healthcare, ref 45152105050250 and 65152105050250) with a beads to sample ratio of 10:1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and mixed with 20 μl of home-made SPRI beads (equivalent of 1 ml of Sera-Mag Magnetic SpeedBeads, carboxylated, 1 μm, 3 EDAC/PA5 (GE Healthcare Life Sciences) in 50 ml of binding buffer containing 10 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Cell Biology 2020Quote: ... Tryptic peptides were desalted using 500 µg of a 1:1 mixture of hydrophobic and hydrophilic Sera-Mag Carboxylate-modified Speed Beads (GE Healthcare Life Sciences).
-
bioRxiv - Bioengineering 2019Quote: ... i) Aptamer selection using biotinylated HGM-box that were immobilized on streptavidin-coated magnetic beads (Streptavidin Mag Sepharose™ from GE Healthcare, USA). The bead binding capacity for either HMG-box Pf or HMG-box Hs was estimated to19 pmol/μL beads slurry ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: we incubated samples for 10 min with Buffer A (1 M GA, 80% ACN, 5% TFA) followed by incubation with the TiO2 Mag Sepharose (GE Healthcare Life Sciences) for 30 min in an Eppendorf Thermomixer at 800 rpm ...
-
bioRxiv - Genomics 2021Quote: ... as follows: The proteinase K-treated reaction was combined with 1 μL of Sera-Mag carboxylate-modified magnetic particles (GE Healthcare, Chicago, IL, USA.) and 72 μL of binding buffer (300 mM NaCl ...
-
bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant was transferred to a new tube with 100 μL of 20% Sera-Mag SpeedBead Carboxylate modified magnetic beads (GE Healthcare Life Sciences, Pittsburgh, PA) in hybridization buffer ...
-
bioRxiv - Microbiology 2021Quote: ... The recombinant RBD was captured by Ni-NTA resin (GE Healthcare) and eluted with 500 mM imidazole in HBS buffer ...