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Citations for GE Life Sciences :
201 - 250 of 1371 citations for Rat Myristoylated Alanine Rich C Kinase Substrate MARCKS ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Unbiotinylated ectodomain transfections were collected and quantified by ELISA using nickel-coated microplates and pooled for purification using nickel-sepharose columns (HisTrap HP, GE Healthcare, Chicago, IL, USA) and an AKTAxpress chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were transferred to Hybond C-Extra nitrocellulose filters (GE Healthcare). Primary antibodies used for immunoblot analysis included ...
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bioRxiv - Bioengineering 2024Quote: ... at 4 °C using an ÄKTA Pure 25 M (GE Healthcare). The column was washed with 75 mM imidazole [in 20 mM NaH2PO4 and 0.5 M NaCl (pH 7.4)] ...
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bioRxiv - Cell Biology 2020Quote: ... the rat cardiomyocytes were purified using a Percoll gradient (0.458 g/ml to 0.720 g/ml; GE Healthcare, Chicago, USA). Cocultures with cdMiPs were performed in a 10:1 ratio (cdMiPs:cardiomyocytes ...
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bioRxiv - Molecular Biology 2020Quote: ... They were then incubated for an hour at RT with 1:5,000 anti-rat horseradish peroxidase conjugated antibody (GE Healthcare). Protein bands were detected on X-ray film using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were then incubated with SuperSignal West Dura or ProSignal Pico Spray chemiluminescence substrate for 1-4 minutes at room temperature and imaged using an Amersham Imager 600 (GE Healthcare) or ChemiDoc (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... The membrane was removed from the substrates and then exposed to the Amersham imager 600 (GE Healthcare Life Sciences, Marlborough, MA).
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bioRxiv - Microbiology 2020Quote: ... diluted 1:10,000 in TBS-T with 3% BSA and finally washed 3 x in TBS-T for 10 min before adding ECL substrate for detection of HRP activity using a CCD camera (ImageQuant, GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: The kinetic studies of the interaction between the Rubisco activase and the substrate Ac-CoA were performed by the surface plasmon resonance (SPR) method using a Biacore T100 instrument (GE Healthcare) at 25 °C as described (Graciet et al. ...
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bioRxiv - Microbiology 2020Quote: ... as primary antibody and goat anti-mouse-HRP conjugated as secondary antibody and ECL prime Western Blotting Detection chemiluminescent substrate (GE Healthcare). The signal was visualized in a Chemidoc MP imaging system (BIO-RAD).
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bioRxiv - Microbiology 2022Quote: ... or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo-Fisher-Scientific)) and chemiluminescent signals were recorded with an Image Quant LAS4000 imaging system (GE Healthcare) or an Amersham Imager 680RGB (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibody was horseradish peroxidase (HRP)-conjugated anti-mouse IgG or IgA and a chemiluminescence HRP was used as substrate (GE Healthcare). Apparent molecular weight of bands was determined using a standard curve generated from molecular weight markers (r2=0.9916 ...
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bioRxiv - Biochemistry 2020Quote: ... The intensity of the gel bands corresponding to the substrate and the product were quantitated with Typhoon TRIO Phosphorimager (GE Healthcare) using ImageQuant TL software (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed and incubated with horseradish peroxidase (HRP)-conjugated goat-anti-rabbit or donkey-anti mouse secondary antibodies for 1h at room temperature followed by chemiluminescence detection using ECL substrate (Pierce) and imaged using Amersham Imager 600 (GE Healthcare). Densitometric band intensity was determined using Amersham Imager 600 analysis software.
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bioRxiv - Neuroscience 2019Quote: ... The blots were exposed to a peroxidase-conjugated anti-mouse secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare, Piscataway, NJ). The resulting chemilluminescent signal was acquired with a Molecular Imager Chemidoc EQ system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were detected with HRP-conjugated goat anti-mouse or anti-rabbit IgG antibody and visualized with the ECL Western blotting substrate (GE Healthcare), according to the provided protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... or ImmunoStar LD (FUJIFILM Wako Pure Chemical) was used as substrate HRP and luminescent signals were detected using an ImageQuant LAS 4000mini (GE Healthcare). To perform re-probing ...
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bioRxiv - Microbiology 2021Quote: ... blots were incubated with WesternPico Super signal substrate (Pierce/Thermo) for detection and chemoluminescence was visualized with X-Ray films (Hyperfilm, GE Healthcare). The following antibodies were used in this work ...
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bioRxiv - Biochemistry 2021Quote: SPR experiments were carried out to characterize the interaction between PKAc and substrate peptides using a Biacore T200 instrument (GE Healthcare). PKAc was immobilized via standard N-hydroxysuccinimide (NHS ...
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bioRxiv - Biochemistry 2021Quote: ... ubiquitylation of fluorescein-labelled substrates was visualized with a fluorescent scan of an SDS-PAGE gel with a Typhoon imager (GE Healthcare) and quantified with ImageQuant (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... HRP-conjugated secondary antibodies were used to incubate the membrane for an hour followed by protein detection with enhanced chemiluminescence (ECL) western blotting substrate and viewed on Amersham Imager 600 (GE Healthcare). Uncropped immunoblot images are shown in Figure S8.
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bioRxiv - Molecular Biology 2022Quote: ... diluted 1:10,000 in TBS-T with 5% powdered milk and finally washed 3x in TBS-T for 10 min before adding ECL substrate (Amersham) for detection of HRP activity using a CCD camera (ImageQuant, GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... the membrane is developed with a chemiluminescent substrate (Super SignalTM West Pico PLUS) and visualized in ImageQuant LAS 4000 mini Biomolecular Imager (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... Finally membranes were again washed three times with TBST and submerged in SuperSignal West Pico chemiluminescent substrate for 2 min before visualizing through ImageQuant LAS 4000 chemiluminescent Image analyzer (GE Healthcare). We used a variety of inhibitors including Torin 1 (inh-tor1 ...
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bioRxiv - Neuroscience 2023Quote: ... Chemiluminescent signals were revealed with SuperSignal West Pico substrate (Pierce) and digitally acquired on a LAS 4000 Mini imaging system (GE Healthcare). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... It was then followed by three times membranes washing in PBST and exposure to either the SuperSignal West Pico PLUS chemiluminescent substrate or the SuperSignal West Femto maximum sensitivity substrate (ThermoScientific) and Images were captured using an ImageQuant LAS4000 mini-Imager (GE Healthcare).
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bioRxiv - Developmental Biology 2022Quote: ... was used as the secondary antibody. Protein was detected using the chemiluminescent substrate ECL (Cat. # 32209, Pierce) in an Amersham Imager 680 (GE Healthcare) detector.
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bioRxiv - Cancer Biology 2023Quote: ... served as chemiluminescent substrates and signals were developed using X-Ray films or imaged on a ProXima 2850 (Isogen) or Amersham 680 (GE healthcare) imaging system ...
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bioRxiv - Developmental Biology 2024Quote: ... Signal detection was obtained with SupersignalTM West Pico Chemiluminescent Substrate HRP and images were acquired using the Amersham Imager 680 RGB (GE Healthcare). Quantifications were performed in Fiji software.
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bioRxiv - Cell Biology 2023Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Crescendo and Forte Reagents and Amersham Imager 600 (GE Healthcare). Quantitative densitometric analyses were performed using ImageJ software.
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bioRxiv - Molecular Biology 2024Quote: ... The protein bands were visualized using an Immobilon Western HRP substrate Crescendo and Forte Reagents and Amersham Imager 600 (GE Healthcare). Quantitative densitometric analyses were performed using ImageJ software.
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bioRxiv - Cell Biology 2024Quote: ... Bands were visualised bu SuperSignal West Fempto ECL substrate (Thermo) and captured using an Amersham ImageQuant 800 imaging system (GE Healthcare). Images were processed using ImageJ(v.1.53 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAi efficiency testing for ELAV was done from dissected central brains by Western blotting according to standard protocols as described 73 using a polyclonal rat anti-ELAV antibody generated against Drosophila ELAV (1:800) 45 and secondary HRP-coupled goat anti-rat antibody (1:5000, GE Healthcare) by chemoluminescence detection (Pierce ...
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bioRxiv - Biochemistry 2021Quote: ... a PCR fragment containing ubiquitin gene and a PCR fragment containing rat parkin residues 77-465 were introduced into the BamHI/XhoI sites of pGEX-6P1 (GE Healthcare). The serine (S77 ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary HRP-conjugated antibodies were purchased from Promega or GE Healthcare (anti-mouse Cat. No. W4021, Promega; anti-rabbit Cat. No. W4011, Promega; anti-rat Cat. No. NA935V, GE Healthcare). All secondary antibodies were diluted in 5% milk-PBS with 0.3% Tween 20 ...
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bioRxiv - Molecular Biology 2020Quote: ... visualized with Supersignal West Pico Chemiluminscent Substrate (Pierce/ThermoScientific; IL, USA) by using the enhanced chemiluminescence imager: LAS400 (GE Healthcare, IL, USA). Membranes were then stripped and reprobed for quantitation of the corresponding total protein or GAPDH to allow for normalization of values based on total protein loading ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein bands were developed using Luminata Classico or Luminata Forte HRP substrate (MilliporeSigma, USA) and detected using Amersham Imager 600 (GE Healthcare, USA).
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bioRxiv - Molecular Biology 2021Quote: ... diluted 1:2000 in 5% BSA using ServaLight Eos CL or EosUltra CL HRP Substrate (Serva Electrophoresis GmbH, Germany) and a LAS ImageQuant 4000 (GE Healthcare, USA). Membranes were then stripped for 10 min in 200 mmol L−1 glycine ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated secondary antibodies were incubated for 1 h at room temperature and membrane development was performed using Enhanced Chemiluminescence (ECL) Western Blotting substrate (GE healthcare, RPN2106) or ECL Prime Western Blotting substrate (GE healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... Signal developing was performed with the Pierce™ ECL Western Blotting Substrate in either a LAS-300 Imager (Fujifilm) or an Amersham Imager 600 (GE Healthcare). The following primary antibodies were used ...
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bioRxiv - Molecular Biology 2019Quote: Purification was performed at 4 °C using His GraviTrap columns (GE Healthcare). Equilibration was done with 10 ml of B-PER II before extracted soluble fractions were applied to the column ...
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bioRxiv - Immunology 2020Quote: ... at 25 °C using a Series S Sensor Chip NTA (GE Healthcare). CSP27 and CSP9 were immobilized on separate channels on the sensor chip surface as per the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2019Quote: ... using a DeltaVision climate control unit (37°C, 5% CO2) (Applied Precision). The kinetics of pre-stress followed by treatment was analysed from images captured at 2 min intervals for 100 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were applied to CM SephadexTM C-25 column (GE Healthcare) equilibrated with distilled water ...
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bioRxiv - Plant Biology 2020Quote: ... Hybridizations were performed at 65°C in Rapid-Hyb buffer (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: SPR experiments were performed at 25°C using Biacore 3000 (GE healthcare) or Biacore T200 (Cytiva) ...
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bioRxiv - Biophysics 2022Quote: ... cells at 32°C with 0.4 mM Isopropyl-D-thiogalactoside (GE Healthcare) for 8 h ...
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bioRxiv - Cell Biology 2023Quote: Images were acquired at 30°C using a Deltavision Elite (Applied Precision) microscope equipped with an Olympus UPlanSApo 60x or 100x (NA1.4 ...