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Citations for GE Life Sciences :
201 - 250 of 1389 citations for IL 9 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The supernatant fraction containing proteins were loaded on 5ml His-Trap column (GE Healthcare) and eluted with a linear gradient of elution buffer containing 40mM to 500mM Imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The crude extract was applied onto a nickel chelation column (His GraviTrap, GE Healthcare), and TasA28-261-His was eluted according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Subsequently hCD14-his was purified on a 5 mL HisTrap Excel column (GE Healthcare) by employing ÄKTA Pure system (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare). The size and purity of the protein preparations were verified by SDS-PAGE and Coomassie Blue staining ...
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bioRxiv - Biochemistry 2022Quote: ... and His(6)GFP –AnkBD proteins were purified using Glutathione Sepharose 4B (GE Healthcare) or Talon® (Clontech ...
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bioRxiv - Biochemistry 2022Quote: ... the His-tagged DnaK protein was purified using a HisTrap HP column (GE Healthcare) with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was desalted using a His Trap Desalting columns from GE Healthcare according to the prescribed protocol and was stored at −80°C with 40 % glycerol.
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified over 1-2 ml His-Trap FF columns (GE Healthcare) with elution buffer containing 250 mM Imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... coli as His-tag proteins and were purified using the IMAC principle (GE healthcare). Following purification ...
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bioRxiv - Biochemistry 2024Quote: ... ZapD and His-Smt3 were separated by chromatography in 5-ml HisTrap (GE Healthcare), then the protein was eluted in a 5-ml HiTrap Desalting column (Healtcare ...
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bioRxiv - Plant Biology 2022Quote: ... The His-MDIS1CD protein is loaded onto a Ni-NTA agarose column (GE Healthcare) and eluted using elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9-Cys dialysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... and applied to a Hi Trap SP HP column (GE Healthcare #17-1151-01) equilibrated with dCas9 dialysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were applied to Nickel-Nitrilotriacetic (Ni-NTA) His-Trap FF column (GE Healthcare) and 6xHis-tagged-mCherry-p62 was eluted via a stepwise imidazole gradient (50 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... GgUox was purified using a 5 mL His-trap Ni-NTA column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was passed through a His Prep FF 16/10 column (GE Healthcare) and washed with 5 column volumes of buffer ...
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bioRxiv - Biophysics 2023Quote: ... Subsequent removal of DTT was achieved with a Hi-Trap desalting column (GE Healthcare) equilibrated in buffer A without β-mercaptoethanol ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a Hi-Trap heparin affinity column (2×5 ml, GE Life Sciences), and eluted with a salt gradient (200–800 mM NaCl) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were purified from the supernatant using a His-trap affinity column (GE Healthcare) with a stepwise elution method ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2% BSA in TBST) incubation followed by chemiluminescence-based detection (Pierce, Rockford, IL) with an Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL) (24).
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bioRxiv - Synthetic Biology 2021Quote: ... then imaged using a DeltaVision microscope (GE Healthcare, Chicago, IL) equipped with a 100x objective (Olympus ...
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bioRxiv - Evolutionary Biology 2021Quote: ... quantified with a NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA), and stored at −80°C ...
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bioRxiv - Systems Biology 2020Quote: ... blots were flooded with ECL (Amersham; GE Healthcare, Chicago, IL) and bands detected using chemiluminescent film (Hyperfilm ECL ...
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bioRxiv - Microbiology 2019Quote: ... Superose 6 10/300 GL (GE Healthcare, Chicago, IL, USA) equilibrated with 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... and a Superdex 200 size exclusion chromatography (GE Healthcare, IL). The peak fractions were concentrated to 0.1 mg/mL for DXT (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... and Superdex 75 gel filtration chromatography (GE Healthcare, Chicago, IL) into 20 mM HEPES buffer pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... M13KO7 helper phage were obtained from GE Healthcare (Chicago, IL). Ampicillin ...
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bioRxiv - Biochemistry 2021Quote: ... gels were soaked in Amplify solution (GE Healthcare, Chicago, IL) for 30 min before drying on a vacuum air dryer ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 ml Sephadex G-50 (GE Healthcare Life Sciences, IL) in order to remove excess NHS-fluorescein.
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bioRxiv - Neuroscience 2019Quote: ... 5% horse serum (HS; GE Healthcare Life Sciences, Chicago, IL), 1% Pen-Strep (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants were incubated with Glutathione Sepharose (GE Healthcare, Chicago, Il) for 90 minutes after which beads were washed with IPB ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1% Penicillin/Streptomycin (#sv30010, HyClone, GE Healthcare, Chicago, IL). The mantle cell lymphoma cell line Maver-1 (#ACC 717 ...
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bioRxiv - Neuroscience 2022Quote: A 3.0T MRI scanner (GE 750; GE Healthcare, Chicago, IL) with equal imaging protocol settings at all time points was applied to acquire axial head 3-dimensional T1-weighted ...
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bioRxiv - Molecular Biology 2019Quote: ... and imager ImageQuant LAS 4000 (GE Healthcare; Chicago, IL, USA). Primary and secondary antibodies used in this study are summarized in Table 3.
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bioRxiv - Plant Biology 2020Quote: ... Horseradish peroxidase –conjugated secondary antibody (GE Healthcare, Chicago, IL, USA) and Amersham ECL (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were visualized using ECL reagent (GE Healthcare, Chicago, IL) and immunoblot intensities were quantified with ImageJ.[32].
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bioRxiv - Plant Biology 2019Quote: ... filled with Sephadex G-50 superfine (GE Healthcare, Chicago, IL) was run on a Bio-Rad BioLogic Duo Flow FPLC system equipped with a QuadTec detector ...
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bioRxiv - Cancer Biology 2020Quote: ... and immobilized onto nitrocellulose membranes (GE Healthcare, Chicago, IL, USA) by electroblot (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... visualized using ImageQuant LAS 500 (GE Healthcare, Chicago, IL, US) then quantified relatively to loading controls by optical density using ImageJ (V1.46d ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were soaked in Amplify (GE Healthcare, Chicago, IL, USA), dried and exposed to films (Carestream Health Medical X-ray Blue ...
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bioRxiv - Physiology 2020Quote: ... or ECL™ Prime reagent kits (GE Healthcare, Chicago, IL). Western blots were first probed with the antibody of interest ...
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bioRxiv - Microbiology 2019Quote: ... and exposed to a phosphor screen (GE Healthcare, Chicago, IL) overnight ...
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bioRxiv - Plant Biology 2020Quote: ... and purified using Glutathione Sepharose 4B resin (GE healthcare, IL). Both MBP-tagged and GST-tagged recombinant proteins were further purified with a Nanosep® 30-kDa size-exclusion column (PALL ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) using pGEX4T-3 vector (GE healthcare, IL).
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bioRxiv - Microbiology 2020Quote: ... or anti-rabbit Ig-HRP (GE Healthcare, Chicago, IL, USA) both diluted to 1:5,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated in streptavidin-Cy5 (GE Healthcare, Chicago, IL, USA) [16] ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were isolated with Ficoll-Hypaque (GE Healthcare, Chicago, IL) density gradient centrifugation ...
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bioRxiv - Plant Biology 2024Quote: ... Horseradish peroxidase-conjugated secondary antibody (GE Healthcare, Chicago, IL, USA) and Amersham ECL (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were obtained from GE Healthcare (Chicago, IL, USA). Bound antibodies were detected by ECL (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... 10% fetal bovine serum (FBS) (GE Healthcare, Chicago, IL, USA), and a mixture of 100 units/ml penicillin and 100 μg/ml of streptomycin (P/S ...