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Citations for GE Life Sciences :
201 - 250 of 647 citations for Human Maspin Highly Pure Recombinant since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The recombinant expressed protein was purified using HisTrap column (GE Healthcare). The His-Sumo tag was digested by ulp1 protease and removed by a second HisTrap column ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins in 10 mM acetate buffer (pH 4.5, GE Healthcare) was then injected over the chip surface at a flow rate of 30 μl/min to couple the amino groups of the recombinant proteins to the carboxymethyl dextran matrix ...
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bioRxiv - Molecular Biology 2021Quote: All recombinant proteins were purified firstly by Ni-NTA (GE healthcare). TOFU-6/PICS-1 was further purified by HiLoad 16/600 Superdex 75 column (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a GSTrap column and elution with 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a HiTrap TALON column and eluted with 50 mM sodium phosphate ...
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bioRxiv - Synthetic Biology 2023Quote: ... recombinant proteins were purified using Ni-Sepharose affinity chromatography (GE Healthcare). Final elution was carried out at 250 mM imidazole ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Recombinant protein was first purified on HisTrap HP column (GE Healthcare) using Buffer A (50mM Tris Base ...
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bioRxiv - Cell Biology 2022Quote: Recombinant GST-NF2 was bound to a GST resin (GE Healthcare), and MBP-NF2 was bound to an MBP resin (New England Biolabs ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples were further purified by gel filtration chromatography on an ÄKTA Pure 25 FPLC (GE Healthcare) using a Superdex75 or Superdex75 increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Bioengineering 2020Quote: ... Complexes were purified on a Superose 6 Increase 10/300 column using an Äkta Pure system (GE Healthcare) in TBS buffer ...
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bioRxiv - Biochemistry 2021Quote: ... samples were loaded onto a S200 10/300 column via direct injection using an Äkta Pure (GE Healthcare) and run at 0.8 mL/min at RT ...
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bioRxiv - Biophysics 2022Quote: ... Then the proteins were purified from the lysate with the Ni2+-NTA column of ÄKTA Pure (GE healthcare). Then the proteins were biotinylated using Avidity biotinylation kit and recommended protocol ...
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bioRxiv - Microbiology 2020Quote: ... His6-SUMO-SepH or His6-SUMO-FtsZMs were purified using an HisTrap column in ÄKTA pure (GE Healthcare) and eluted using an increasing concentration of imidazole ...
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bioRxiv - Bioengineering 2020Quote: ... complexes were purified on a Superose 6 Increase 10/300 column using an Äkta Pure system (GE Healthcare) in PBS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... NM- His was purified from the supernatant via IMAC using the ÄKTA pure protein purification system (GE Healthcare) together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... The cleared lysate was then injected into the equilibrated column connected to ÄKTA pure purification system (GE Healthcare). We did not observed the clogging of the column and the system ...
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bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were injected and run through a ÄKTA Pure chromatography gel filtration system (GE Healthcare Life Sciences) equipped with a Superdex 75 Increase 10/300 GL column (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... The Vag8 was eluted with a linear gradient of imidazole on an FPLC system (ÄKTA pure, GE Healthcare) using buffer B and buffer C (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Cell-free supernatant was applied to the column at 2 mL/min using an AKTA Pure (GE Healthcare), washed ...
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bioRxiv - Biophysics 2024Quote: ... Fractions containing >95% pure RyR1 were pooled and further purified with HiTrap Heparin HP Agarose column (GE Healthcare), after a five-fold dilution step ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions corresponding to pure RIP2K were then pooled and applied onto a PD-10 desalting column (GE Healthcare) equilibrated with buffer C (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing pure KIT or KIT:SCF dimers were gently concentrated using a 100 kDa MWCO concentrator (GE Healthcare) at 100×g to a concentration of 6.5 mg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... and buffer exchanged in PBS before a final FPLC purification step (ÄKTA pure instrument; GE Healthcare Life Sciences) via a small-scale size exclusion chromatography column (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Cell Biology 2020Quote: ... Size exclusion chromatography was performed in storage buffer at room temperature on an ÄKTA Pure 25L1 system (GE Healthcare). Elution of proteins was monitored at 280 nm ...
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bioRxiv - Cell Biology 2020Quote: ... and size exlusion chromatography (SEC) was performed at 0.9 ml/min in 0.22 μm-filtered 1x PBS with an Äkta Pure (GE healthcare) FPLC system ...
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bioRxiv - Microbiology 2021Quote: ... and further purified using size exclusion chromatography (SEC) on an AKTA pure 25L protein purification system (GE Healthcare, USA) in buffer C (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: Multi-angle light scattering coupled size-exclusion chromatography (SEC-MALS) was performed using an Äkta PURE system (GE Healthcare) with a Superdex 200 Increase 10/300 column attached to a MALS detector 3609 (Postnova Analytics ...
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bioRxiv - Physiology 2021Quote: ... Fractions containing >95% pure RyR1 were pooled and further purified with a HiTrap Heparin HP Agarose column (GE Healthcare), after a five-fold dilution and filtration step ...
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bioRxiv - Plant Biology 2021Quote: ... soluble proteins were purified by affinity chromatography using a HisTrap Ni-NTA column (ÄKTA pure FPLC system, GE Healthcare) equilibrated in 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... using an inline size-exclusion chromatography set-up (AKTA pure with Superdex 200 10/300 GL column; GE Healthcare). Two-dimensional scattering data were radially averaged ...
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bioRxiv - Biochemistry 2022Quote: ... fractions containing pure protein sub-complexes were subjected to size-exclusion chromatography (Superose 6 increase 10/300, GE Healthcare) in 25 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2020Quote: ... All purifications were carried out on an Äkta™ start or Äkta™ pure chromatography system (GE Healthcare, USA). For the purification of free mSOG the unbound fraction obtained during the IMAC purification of Enc-mSOG was used ...
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bioRxiv - Immunology 2021Quote: ... The beads were then packed in a column coupled to an AKTA™ Pure system (GE Healthcare, Life Sciences). HypR was eluted using a linear gradient with elution buffer ...
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bioRxiv - Immunology 2020Quote: ... Approximately 10mg of DH717.1 Fab was loaded onto a Superdex 200 increase 10/300 column using a 500μL loop and run at 0.5 mL/min using an Äkta Pure system (GE Healthcare). DH717.1 Fab peaks were collected via fractionation using a 96-well plate and were analyzed using the Unicorn 7.0.2 software (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... size-exclusion (SEC) column connected to an ÄTKA™ pure fast protein liquid chromatography (FPLC) system (GE Healthcare Inc.). The column was equilibrated with SEC buffer (50 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... The trimer fraction was purified by size exclusion chromatography on a Superdex 200 10/300 GL column in TBS + 0.1% DDM + 0.003% sodium deoxycholate using an ÄKTA Pure 25L HPLC instrument (GE Healthcare) at a flow rate of 0.75 ml/min.
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Design and Evaluation of Nanoscale Materials with Programmed Responsivity towards Epigenetic EnzymesbioRxiv - Bioengineering 2024Quote: ... and pure HDAC8 enzyme was obtained using the HisTrap column on AKTA purifier UPC 10 (GE Healthcare Life Sciences). SDS-PAGE agarose gel and catalytic activity analysis confirmed the presence of the HDAC8 enzyme ...
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bioRxiv - Microbiology 2024Quote: ... 30 min) and loaded onto a 5 ml HighTrap MBP column (Cytiva) using an Äkta-Pure system (GE Healthcare). The column was washed with 10 column volumes of lysis buffer and protein eluted with MalE-elution buffer (100 mM Tris-HCl pH7.2 ...
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bioRxiv - Physiology 2023Quote: ... 2500µg of cleared mitochondrial lysate were fractionated by fast protein size-exclusion liquid chromatography (AKTA Pure FPLC; GE Healthcare), using a Superose 6 Increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: All chromatography steps were performed at 4 °C using the ÄKTAxpress and ÄKTA pure protein purification systems (GE Healthcare). For affinity purification of the ADGRG4 construct ...
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bioRxiv - Synthetic Biology 2022Quote: ... The soluble fraction was loaded onto a 5 mL Ni-NTA column connected to an ÄKTA pure (GE Healthcare) chromatography system ...
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bioRxiv - Biophysics 2023Quote: ... Buffer conductivity was measured on the built-in conductivity monitor of the chromatographic system AKTA Pure 150M (GE Healthcare). The sample was kept at 4 °C no more than 7 d ...
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bioRxiv - Evolutionary Biology 2024Quote: We performed size-exclusion chromatography of His-tag purified coGFP variants on an Äkta Pure chromatography system (GE Healthcare), with a Superdex 75 10/300 Increase column (GE Healthcare ...
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bioRxiv - Bioengineering 2024Quote: ... and fractionated by size-exclusion through three tandem superdex 200 increase columns on an Akta Pure FPLC (GE Healthcare). The siRNA content of each fraction was measured with a plate reader (Biotek Synergy H1 ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant expressed protein was purified using a HisTrap column (GE Healthcare). The hexahistidine plus MBP tag was removed by TEV protease digestion followed by a second step HisTrap column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant expressed protein was purified using a HisTrap column (GE Healthcare). The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified on a HisTrap HP column (GE Healthcare), followed by buffer exchange on a HiPrepTM 26/10 desalting column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant protein was then isolated using Ni-NTA resin (GE healthcare) and eluted with a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing recombinant was purified on TALON Superflow resin (GE healthcare) per manufacturer’s instructions ...