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Citations for GE Life Sciences :
201 - 250 of 1288 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... gels were firmly attached to one of the two glass gel plates via binding silane (GE Healthcare, Uppsala, Sweden) whereas the opposite plate was coated with a releasing agent (Blueslick ...
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bioRxiv - Biochemistry 2020Quote: The binding affinity of GDAP1Δ319-358 and GDAP1Δ295-358 towards HA was measured using a MicroCal iTC200 calorimeter (GE Healthcare) in SEC buffer with 2% (v/v ...
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bioRxiv - Biophysics 2021Quote: SecA:lipid binding experiments were performed using L1 sensor chip on two-channel Biacore X100 instrument (GE Healthcare Life Sciences). DOPC-only liposomes lacking SecA interaction were employed in the reference channel ...
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bioRxiv - Cell Biology 2021Quote: Sensorgrams of lncEry-P5 binding to Wdr82 were measured by SPR technology using a Biacore 3000 instrument (GE Healthcare). The flowing-phase RNA lncEry-P5 was synthesized by in vitro transcription using a T7 RNA production system (Promega ...
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bioRxiv - Immunology 2021Quote: Kinetics binding measurement for anti-spike mAbs in this study was performed using a Biacore T200 instrument (GE Healthcare). The system was flushed with filtered 1xHBS-P+ running buffer (0.01M HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The first step was affinity purification on an MBP-binding dextrin Sepharose column (MPBTrap, GE Healthcare: 28-9187-78). The lysates were loaded twice on the column at a flow rate of 0.5 ml/min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gravity IMAC was performed by batch binding the clarified supernatants with 10mL of Ni Sepharose Excel resin (GE Healthcare). After 20-30 minutes of incubation ...
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bioRxiv - Immunology 2023Quote: ... Binding responses were measured following immobilization by amine coupling of antigens (37) on CM5 sensor chips (BIAcore/GE Healthcare). Purified plasma IgG samples at 200 µg/ml were flowed (2.5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA binding was measured by capturing protein-RNA complexes on Protran nitrocellulose membrane (Whatman, GE Healthcare Bioscience, Pittsburgh, PA) and unbound RNA on a Hybond-XL (Cytiva ...
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bioRxiv - Biophysics 2021Quote: ... and the excess small molecular was removed by Superdex peptide size-exclusion column (GE Healthcare) equilibrated in refolding buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Cross-linked peptides were enriched in a Superdex 30 Increase 3.2/300 column (GE Healthcare) equilibrated with 30% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Crosslinked peptides were enriched by size exclusion chromatography using an ÄKTAmicro chromatography system (GE Healthcare) equipped with a Superdex™ Peptide 3.2/30 column (column volume = 2.4 ml) ...
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bioRxiv - Microbiology 2021Quote: ... the peptide was purified with size-exclusion chromatography using a Superdex 75 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Na HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
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bioRxiv - Immunology 2020Quote: ... The equilibrium Kd was determined by fitting the data to a steady-state 1:1 binding model using Biacore X100 Evaluation software version 2.0 (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Flow cell 1 was left empty to use this flow cell as a reference flow-cell for on-line subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface using Biacore T100 Control Software (version 3.2) (GE Healthcare). FPLC-purified non-biotinylated protein was used as analyte ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was then purified from the supernatant using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) in the presence of 0.1 mM DTT ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: NAB2 binding to GST-Nedd4 and Nedd4(HECT) was characterized via SPR using the Biacore T200 instrument (GE Life Sciences). Recombinant protein was purified as described with an additional purification step using size exclusion chromatography to ensure high purity prior to use in SPR ...
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bioRxiv - Immunology 2020Quote: ... Background binding to blank immobilized flow cells was subtracted and affinity constants were calculated using BIA Evaluation software (GE Healthcare) using the Steady State Affinity model ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Microbiology 2020Quote: ... and the kinetic parameters were obtained by globally fitting the double-reference subtracted data to a 1:1 binding model with mass transport limitation using Biacore T200 Evaluation software v 3.1 (GE Healthcare). The dissociation rate constant (kd ...
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bioRxiv - Cell Biology 2020Quote: ... This was followed by performing SEC using GST binding buffer supplemented with 0.5 mM TCEP on a Superdex 200 10/300 column using an ÄKTA purifier (GE Healthcare). The fractions corresponding to the peak were pooled ...
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bioRxiv - Biochemistry 2022Quote: ... fits of the sensorgrams were determined using a 1:1 binding model (Langmuir) using Biacore T200 Evaluation Software 3.1 (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... Flow cell 1 was left empty to use this flow cell as a reference flow-cell for on-line subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface using Biacore T100 Control Software (version 3.2) (GE Healthcare). FPLC-purified non-biotinylated protein was used as analyte ...
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bioRxiv - Immunology 2022Quote: ... and the raw data curves were fitted to a 1:1 binding model using Biacore 8K Evaluation Software (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: The binding affinity of b96.11 to apo- and holo- GAD65 was measured using surface plasmon resonance (BIAcore T-100, GE Healthcare). Purified monoclonal b96.11 IgG antibody was captured on a CM5 sensor chip with immobilised Protein G ...
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bioRxiv - Biophysics 2023Quote: ... and the binding affinities were obtained using a 1:1 (Langmuir) binding fit model with Biacore T200 Evaluation software version 3.2 (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... Batch binding was performed overnight with Glutathione resin (Glutathione SepharoseTM 4 Fast Flow, GE Healthcare Cat. no. 17-5132-02) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... All kinetic profiles were fitted using a global 1:1 binding algorithm with a drifting baseline using BIAevaluation v.3.1 (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The cargo tail peptides were isolated on gravity-flow column containing Ni-NTA agarose (GE Healthcare) followed by gel-filtration chromatography on Superdex Peptide 10/300 (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The eluent was then separated from the Cy3-peptide using a second heparin column (GE Healthcare) and polished using a MonoQ 5/50 GL (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... Peptide fraction was performed on AKTA pure 25 with Superdex 30 Increase 3.2/300 (GE Healthcare) at a flow rate of 30 μl min-1 of the elution buffer with a 100 μl fraction volume ...
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bioRxiv - Genomics 2024Quote: ... The mixture of labeled peptides was fractionated using strong cation exchange beads (Source 15S, GE Healthcare). After the sample was applied ...
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bioRxiv - Cell Biology 2020Quote: ... GFP fusions were immunoprecipitated using lab-purified GFP binding protein (GBP) (Rothbauer et al., 2008) coupled to NHS-activated Sepharose FastFlow beads (GE Healthcare) using a protocol adapted from (Babiano et al. ...
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bioRxiv - Microbiology 2021Quote: ... Kinetic analysis was performed on each injection series using a 1:1 (Langmuir) binding model was applied using Biacore T200 Evaluation Software (GE Healthcare). Equilibrium dissociation constants (KD ...
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bioRxiv - Biochemistry 2022Quote: ... including a reference channel for online background subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface (Biacore T100 Control Software; GE Healthcare). All measurements were performed with 2-fold serial dilutions using 60 s association followed by a dissociation time of more than 240 s at 25 °C with a flow rate of at 30 μl min−1 ...
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bioRxiv - Immunology 2021Quote: ... PPACK-thrombin or fibrinogen in acetate buffer pH4.5 was immobilized to a chip surface (1000 or 10000 RU) using NHS/EDC-amine coupling and residual binding sites blocked by 1M ethanolamine (GE Healthcare). FH (0.5-62nM ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the protein from the soluble fraction was done in Binding buffer on a 1 mL His Trap Column (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Kinetic measurements of the DH1047 Fab binding to SARS-CoV and RsSHC014 spike proteins were obtained using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Microbiology 2021Quote: ... assay was performed to determine the binding affinities of aCPSF1 to poly-U-tract containing RNA on a BIAcore 8K instrument (GE Healthcare) as described previously (Zhang et al. ...
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bioRxiv - Biophysics 2022Quote: Binding kinetics of ACE2 and SARS-CoV-2 RBDs were determined by surface plasmon resonance using a Biacore S200 (GE Healthcare). All experiments were performed in a running buffer composed of 10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Immunology 2020Quote: ... Ab binding (protein expression) was visualized using Amersham ECL Prime Western blotting detection reagent (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). The following Abs were used in this study ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then diluted 10 times with mono Q binding buffer (50 mM HEPES, pH 8.0) and applied to a MonoQ 5/50 GL column (GE Life Sciences) connected to an AKTA Pure system (GE Life Sciences). ...
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bioRxiv - Biochemistry 2020Quote: ... Fluorescence was detected where binding occurred between SKI and PSMAD3–SMAD4 complex and measured with a Typhoon FLA 9500 biomolecular imager (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... assay was performed to quantify the binding avidity of the recombinant mos-tri-RBD to the receptor hACE2 using BIAcore 8K (GE Healthcare) with NTA chips ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA-protein binding complex was resolved on SDS-PAGE prior to analysis by Coomassie staining and PhosphoImaging (Typhoon FLA 7000, GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... The protein binding complex was separated on SDS-PAGE prior to analysis by Coomassie staining and Phosphoimaging (Typhoon FLA 7000, GE Healthcare). For pull down with purified PGC-1α protein domains ...
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bioRxiv - Microbiology 2021Quote: ... Both kinetic and equilibrium binding data were analyzed in the manufacturer’s Biacore 8K evaluation software version 1.1 (GE Healthcare, Chicago, IL). All experiments were performed at 37 °C in HBS-EP.