Labshake search
Citations for GE Life Sciences :
201 - 250 of 905 citations for 7 bromo 3 hydroxy naphthalene 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
-
bioRxiv - Biochemistry 2024Quote: ... Removal of nucleic acid contaminants was performed using 1 ml or 5 ml HiTrap Heparin column (GE Healthcare) and eluted using a NaCl gradient from 50 mM to 1 M ...
-
bioRxiv - Physiology 2020Quote: ... Examinations were carried out with a linear array i13L probe (10-14 MHz) using a Vivid 7 Dimension ultrasound system (GE Healthcare Ultrasound, Horten, Norway). The operator was blinded to the genotype of the mice ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.3 mM TCEP and subsequently concentrated (Vivaspin 20 3 kDa MWCO, GE Healthcare) to ~10 mg/mL ...
-
bioRxiv - Cancer Biology 2022Quote: ... or pH 3–10 for CDK2 (Amersham Biosciences, GE Healthcare Europe, Diegem, Belgium) before separation by SDS-PAGE (42 ...
-
bioRxiv - Cell Biology 2022Quote: ... HUVEC were coated onto cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) and embedded in a fibrin matrix by combining 7µl of 50U/ml thrombin (T7201-500UN ...
-
bioRxiv - Cancer Biology 2020Quote: ... 3 μm pore-size polycarbonate filters (Whatman, GE Healthcare Europe GmbH, Milan, Italy) previously pre-coated with 2 μg/mL of fibronectin ...
-
bioRxiv - Molecular Biology 2020Quote: ... Supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) equilibrated with lysis buffer with imidazole concertation brought up to 50 mM and suspension was applied on Flex-Column (420400-2510 ...
-
bioRxiv - Immunology 2022Quote: ... and 3 mL of 66% percoll was underlaid (GE healthcare 17–0891–01). Samples were spun for 20 min at 2000 rpm at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... in the 24-fraction setup using pI 3-10 IPG strips (GE Healthcare) for 50 kVh ...
-
bioRxiv - Biochemistry 2023Quote: ... blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare), and plunge frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... acetonitrile (ACN) and 0.1% (v/v) trifluoro acetic acid (TFA) using a Superdex Peptide PC 3.2/30 column (GE Healthcare). After evaporating to dryness and dissolving in 4% (v/v ...
-
bioRxiv - Immunology 2020Quote: ... acid-eluted immunoprecipitates were separated by SDS-PAGE under reducing conditions and transferred to HybondTM ECLTM membranes (GE Healthcare). Membranes were incubated sequentially with the different primary antibodies and developed with the appropriate HRP-conjugated secondary antibody using enhanced chemiluminescence (ECL) ...
-
bioRxiv - Biophysics 2019Quote: ... its only cysteine at amino acid #265 near the flexible C terminus was labeled with Cy5 maleimide (GE healthcare) or AlexaFluor488 C5 maleimide (Thermo Fisher ...
-
bioRxiv - Microbiology 2022Quote: Southern blots were accomplished following the specifications of ECL Direct Nucleic Acid Labeling and Detection System (GE Healthcare, UK). Briefly ...
-
bioRxiv - Microbiology 2020Quote: ... DksA was exchanged into a buffer containing 15 mM sodium phosphate and 100 mM NaCl (pH 7) over a PD-10 Sephadex column (GE Healthcare Bio-Sciences, Pittsburg, PA). DksA protein solutions (12.5 μM ...
-
bioRxiv - Pathology 2021Quote: ... 250 μg of total protein was loaded on a 13 cm Immobiline dry-strip (pH 4-7, M/s. GE Healthcare Life Sciences, Amersham, UK), on a rehydration tray ...
-
bioRxiv - Biochemistry 2023Quote: ... 55 µg of protein from pulse-labeled samples for analytical gels and 300 µg of protein from unlabeled samples for preparative gels were rehydrated into IPG strips (24 cm, pH 4-7; GE Healthcare, Chalfont St Giles, UK). A Multiphor II (GE Healthcare ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 mM L-Glutamine (GE Life Sciences #SH30852.01), 100 units/mL Penicillin and 100 μg/mL Streptomycin (Corning #30-001-CI ...
-
bioRxiv - Cell Biology 2021Quote: ... GST-hEBP50 in pGEX-6P-2 (GE Healthcare): GST-hEBP50/WT (AA95-168) ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 mM βME using Glutathione Sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 mM βME using glutathione sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
-
bioRxiv - Cancer Biology 2022Quote: ... siRNA non-targeting pool #2 (GE Healthcare Dharmacon) was used as a control for all the knockdown experiments ...
-
bioRxiv - Neuroscience 2020Quote: ... the 2-D Clean-Up Kit (GE Healthcare) was used following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... (2) Ni Sepharose fast flow resin (GE Healthcare) chromatography using a step elution from 50 mM to 200 mM Imidazol in Tris buffer B (20 mM Tris/HCl ...
-
bioRxiv - Cell Biology 2019Quote: ... 2 mM βME using glutathione sepharose (GE Healthcare). Protein was cleaved overnight using thrombin (Recothrom ...
-
bioRxiv - Microbiology 2023Quote: ... 2 µl of either anti-GST (GE Healthcare), anti-HA (Sigma H6908) ...
-
bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare, Vélizy-Villacoublay, France), 0.5% penicillin-streptomycin (Invitrogen)) ...
-
bioRxiv - Neuroscience 2021Quote: ... Solubilized supernatant was isolated by ultracentrifugation and the supernatant was incubated for 2 h with 2 mL CNBR-activated Sepharose beads (GE healthcare) coupled with 4 mg high-affinity GFP nanobodies (2) ...
-
bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated for 2 h in Can Get Signal Solution 2 (Toyobo) with anti-rabbit or mouse IgG conjugated to horseradish peroxidase (GE Healthcare). After washing twice with TBST ...
-
bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S-2P and SARS-CoV-2 HexaPro S were purified over a Superose6 Increase column (GE Life Sciences).
-
bioRxiv - Cell Biology 2020Quote: ... infected cells were coated onto cytodex 3 microcarrier beads (17048501, GE Healthcare Life Sciences) and embedded in a fibrin matrix as described ...
-
Increasing phagocytosis of microglia through targeting CD33 with liposomes displaying glycan ligandsbioRxiv - Immunology 2021Quote: ... the pellet was dissolved in 3 ml of 30% Percoll (Percoll PLUS, GE Healthcare) and carefully layered on top of 70% Percoll and immediately centrifuged (650 rcf for 20 min) ...
-
bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
-
bioRxiv - Biophysics 2021Quote: ... followed by concentrating the sample (VivaSpin 6 MWCO 3 kDa cut-off, GE Healthcare) to 8 μM ...
-
bioRxiv - Biochemistry 2020Quote: ... A volume of 3 mL of Glutathione Sepharose 4B resin (17075605, GE Life Sciences) was added to the soluble fraction and incubated for a minimum of 6 hours at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... chromatin lysates were incubated for 3 hours with protein-G sepharose beads (GE Healthcare). Beads were washed 3× with low-salt buffer (150 mM NaCl ...
-
bioRxiv - Cell Biology 2022Quote: ... were pooled and coupled with either Cy®3 Mono NHS Ester (GE healthcare) or Alexa FluorTM (AF ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare) cDNA of the reference pool were then hybridized together on customized microarray chip using commercially available hybridization platform (Agilent ...
-
bioRxiv - Biophysics 2023Quote: ... The supernatant was mixed with 3 ml of Ni2+ Sepharose (GE Healthcare Life Sciences) which had been equilibrated with lysis buffer supplemented to 50 mM imidazole pH 8.0 ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
-
bioRxiv - Biochemistry 2024Quote: ... GST-tagged constructs were cloned into a modified form of pGEX4T-3 (GE Healthcare) with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV ...
-
bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
-
bioRxiv - Immunology 2021Quote: ... The supernatant was then loaded onto the protein A column and the target protein was eluted with 0.1 M acetic acid on ÄKTA pure (GE Healthcare). The collected protein was added to 1 mM Edetate disodium (EDTA) ...
-
bioRxiv - Cell Biology 2022Quote: ... RNA was then purified from the supernatant using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) in the presence of 0.1 mM DTT ...
-
bioRxiv - Microbiology 2021Quote: ... The cleared extract was filtered (0.45 μm) before loading onto a nickel-nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated with lysis buffer ...
-
bioRxiv - Immunology 2020Quote: ... running on a mobile phase buffer of buffer A (0.1% trifluoroacetic acid (TFA)) and buffer B (80% acetonitrile/0.1% TFA) on an AKTAmicro™ HPLC system (GE Healthcare). The separation was performed with a rapid gradient of buffer A to B (2 to 40% B for 4 mins ...
-
bioRxiv - Bioengineering 2019Quote: ... The nucleic acids were visualized using a G:BOX Chemi imager (Syngene, USA) and quantitative analyzed by ImageQuant (GE Healthcare, USA).
-
bioRxiv - Molecular Biology 2021Quote: ... in water /0.1% (v/v) trifluoroacetic acid (TFA) and crosslinked peptides were enriched by peptide size exclusion chromatography (SuperdexPeptide 3.2/300 column, GE Healthcare) with a flow rate of 50 μl/ min in 30 % (v/v ...
-
bioRxiv - Cell Biology 2020Quote: The amino acids 77-136 encoding the cytoplasmic region of the Hoka protein were cloned into pGEX-6P (GE Healthcare) to produce a GST (Glutathione S-transferase ...