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Citations for GE Life Sciences :
201 - 250 of 4975 citations for 4 4 Fluoro 2 hydroxyphenyl methyl 2 6 bis 1 methylethyl 5 propyl 3 pyridinemethanol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... purified PIN1 and sybody-21 were mixed together with a molar ratio of ∼1:3 and then applied to size-exclusion chromatography using a Superose 6 Increase column (GE Healthcare) in buffer containing 25 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... The blots were visualised with the BioRad ChemiDoc XRS+ (Cohort 2) or the Amersham 6000 Gel Imager (GE Healthcare, Cohort 3), and quantified with Image Lab Software (BioRad) ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified over 1-2 ml His-Trap FF columns (GE Healthcare) with elution buffer containing 250 mM Imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin was precleared for 1 hour at 4 °C with blocked protein A Sepharose beads (GE Healthcare, Eindhoven ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the supernatant was applied to a 1 ml Ni-NTA HisTrap column (GE Healthcare). The protein was eluted with an imidazole gradient using an analogous gradient of HisTrap buffer B (HisTrap buffer A with 500 mM imidazole ...
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bioRxiv - Immunology 2021Quote: Total IgG was initially purified from 4 mL of the multigravidae plasma pool using Protein G Sepharose 4 Fast Flow resin (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The pellet was re-suspended in 4 ml PBS prior to layering over 4 ml of Ficoll solution (GE Healthcare life sciences, UK) in a 15 ml centrifuge tube and centrifuged at 1400 x g for 45 minutes ...
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bioRxiv - Plant Biology 2022Quote: Leaf samples from ino-4 and ino-4 i20 plants were separately pooled and DNA extracted with Nucleon Phytopure DNA extraction kit (GE Healthcare, Pittsburgh, PA), according to the provider’s instructions ...
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bioRxiv - Pathology 2024Quote: Cardiac MRI was performed prior to treatment at baseline (4 weeks post-MI) and at 4 weeks post-treatment on a 3T Premier scanner (GE Healthcare, Waukesha, WI). Specifically ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 mM 2-mercaptoethanol for 12 h and purified by anion exchange chromatography (HiTrap Q, GE Healthcare) with a linear gradient of 0 to 500 mM NaCl over 40 min and size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 5 mL Ni2+ Sepharose 6 Fast Flow (GE Healthcare) was transferred to a 20 mL gravity flow column ...
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bioRxiv - Plant Biology 2021Quote: ... GFP or mCherry fluorescence was measured 4-5 days post-infiltration (see Table S3 for raw data) using the ImageQuant LAS 4000 luminescent imager (GE Healthcare Life Sciences) with the appropriate excitation and emission settings (EX ...
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bioRxiv - Biophysics 2020Quote: ... DCS experiments with working buffer solutions containing 4 μM of an SBD-mutant and 5 mM substrate were conducted on a VP-DSC Calorimeter (Micro Cal™, GE Healthcare, Buckinghamshire). The melting temperature Tm was determined by ORIGIN 8 (Origin Lab Corp ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting supernatant was passed through either a 0.45 or 0.8 μm pore size filter and loaded onto a 5 mL Ni+2-NTA HisTrap HP column (GE Healthcare) pre-equilibrated with binding buffer (20 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Neuroscience 2021Quote: ... using the 14N(p,α)11C route and converted to either [11C]MeI (methyl iodide) or [11C]MeOTf (methyl triflate) using a Tracerlab FX MeI module (GE Healthcare). A Tracerlab FX M module (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Biophysics 2021Quote: ... followed by concentrating the sample (VivaSpin 6 MWCO 3 kDa cut-off, GE Healthcare) to 8 μM ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution in 3% milk overnight at 4°C before incubation with secondary HRP-linked sheep anti mouse (NA931-1ML, GE Healthcare Life Sciences, Buckinghamshire, UK) at a 1:3000 dilution in 5% milk for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... chromosomal DNA was purified and amplified to 5.4 - 7.9 μg by multiple displacement amplification (Supplementary Table 3) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by Šimková et al ...
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA constructs targeting mouse ezrin (5’-ATTTCCTTGTTATAATCTCCG-3’) in a pLKO-puro.1 vector from GE Healthcare and described in27 ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins from input and eluates were separated on 4-20% or 4-18% home-made SDS-PAGE and transferred onto PVDF membranes (GE Healthcare, Chicago, Illinois, USA) followed by detection using HRP conjugated primary anti-HA (clone 3F10 ...
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bioRxiv - Molecular Biology 2020Quote: ... on Protein G Sepharose 4 Fast Flow beads (GE healthcare). The samples were incubated at 4°C overnight and then washed with progressively more stringent conditions ...
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bioRxiv - Biophysics 2021Quote: ... at 4°C using AKTA Pure HPLC system (GE Healthcare). The column was washed with 20 mM NaPi (pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... and Glutathione Sepharose 4 Fast Flow (GE Healthcare Life Sciences), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... conjugated to Protein G SepharoseTM 4 Fast flow (GE Healthcare) and Protein A SepharoseTM 4 Fast flow (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... and treated for 4 h with PreScission protease (GE Healthcare). The TAP-ESP beads were used directly ...
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bioRxiv - Cell Biology 2024Quote: ... Glutathione(GSH)-Seρharose 4 Fast Flow was from GE Healthcare. FLAG peptide (DYKDDDDK ...
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bioRxiv - Biochemistry 2023Quote: ... the binding affinity of fragments 1 – 4 and SlpB were evaluated with Biacore X100 (GE healthcare, Uppsala, Sweeden) with sensor chip protein G (Cytiva ...
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bioRxiv - Cancer Biology 2021Quote: ... with 2% cosmic calf serum (GE Healthcare), 25 ng/mL EGF (R&D ...
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bioRxiv - Microbiology 2020Quote: ... containing 1 mM 2-ME) and Superdex 200 Increase 10/300 GL column (GE Healthcare; 10 mM KPB [pH 7.0] containing 50 mM NaCl and 1 mM 2-ME) ...
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bioRxiv - Plant Biology 2022Quote: ... The primary antibody was diluted 1:10,000 in TBST-Buffer with 2% Blocking (GE Healthcare) and incubated on the membrane for 2 h ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Plant Biology 2024Quote: ... at a 1 to 20 ratio in Buffer A overnight at 4°C followed by a subtractive His trap purification using a 5 mL HisTrap HP column (GE Healthcare, Little Chalfont, UK) (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... 5 ml of Ni2+-beads slurry (Ni-sepharose 6 Fast Flow, GE Healthcare) were washed in washing buffer (20 mM TrisHCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: Analytical SEC was performed using Superose 6 5/150 GL column (GE Healthcare) in a buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... A Superose 6 Increase 5/150 size exclusion chromatography column (GE Healthcare Lifescience) was used to remove thrombin and exchange the buffer to αCSF90 buffer (70 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) containing 5% skim milk (Meiji) and subjected to primary antibody for 2 h and HRP-conjugated secondary antibody (GE Healthcare) for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... Peak fractions were pooled, diluted ~5 fold with buffer 0 (20 mM HEPES, 2 mM DTT) and loaded onto a Q column (GE Healthcare). Rad33 was then eluted from the Q column using a linear gradient of Q buffer A (20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots were taken every 30 minutes for a period of 2 hours and quenched in 3ml of 5% trichloroacetic acid (TCA) on Whatman-glass fiber filters (GE healthcare) and washed 3 times with 1ml ethanol to dry the filter ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 mM DTT and loaded on a 5 ml HiTrap™ Phenyl Sepharose High-Performance column (GE Healthcare, Munich, Germany). The sample was eluted with a 1.0 to 0.4 M linear gradient of (NH4)2SO4 for 8 CV at a flow rate of 1 ml.min-1 ...
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bioRxiv - Biochemistry 2023Quote: ... plate 2 - 1.6 μg/μL carboxylate beads (1:1 mixture of hydrophilic and hydrophobic beads [mass/mass], GE Healthcare Cat ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Immunology 2020Quote: ... Dilution series of TBK1 or IRF-3 (1.25, 2.5, 5, 10, 20 μM) in 1× HBS-EP+ buffer (GE Healthcare) were injected over the sensor chip at a flow rate of 30 μL/min ...