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Citations for GE Life Sciences :
201 - 250 of 4107 citations for 2R 5S 5 4 amino 5 fluoro 2 oxopyrimidin 1 2H yl 1 3 oxathiolan 2 yl methyl butyrate WXC04778 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted and further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... the H3 and H4 monomers were purified using a mono-Q HR 5/5 column (GE healthcare) and SP ion exchange resin (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... All the nucleosomal DNA fragments were purified using ion-exchange chromatography (Source Q 5/5, GE Healthcare) and isopropanol precipitation ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluate was further purified by chromatography on a Mono Q HR 5/5 column (GE Healthcare) with a linear gradient of 0.1-1.0 M sodium chloride in Nap1 chromatography buffer (20 mM Tris-Cl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... see SI Table 5): Clarified lysate was applied to a 5 mL HisTrap FF column (GE Healthcare) equilibrated with His-binding buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... The reduced 5′ thiol-modified ssDNA was purified into water using NAP-5 columns (GE Life Sciences). The reduced 5’ thiol-modified ssDNA was spin vacuum dried ...
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bioRxiv - Neuroscience 2022Quote: ... and an aliquot of synaptosomal suspension (2 ml) was placed onto a 3-ml Percoll discontinuous gradient (GE Healthcare) containing (in mM ...
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bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR at 10 mg mL-1 (340 μM) was injected (70 μL) onto an inline Superdex S200 5/150 Increase (GE Healthcare), equilibrated with buffer C ...
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bioRxiv - Molecular Biology 2020Quote: Wild type or ΔN20 hMcm10-ID (200 µL at 5 mg ml-1) was injected into Superdex 75 Increase 10/300 GL column (GE Healthcare) operating with the elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved KIX was purified using a linear gradient of 50 mM to 1 M NaCl over ten column volumes by cation exchange chromatography using MonoS 5/50 GL column at a flow rate of 1 mL per minute (GE Healthcare). Fractions containing purified KIX were confirmed by SDS-PAGE and dialyzed against 1 L of 50 mM potassium phosphate pH 5.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was next loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... The eluate was then loaded onto a Mono Q 5/50 GL 1 mL column on an AKTA FPLC (GE Healthcare). The column was washed with 5 mL of Buffer A (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: The solution containing the proteins was loaded on a prepacked 1 ml or 5 ml His Trap HP Nickel column (GE Healthcare), which had been washed with 5 column volumes of water and equilibrated with 5 column volumes of buffer A (20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation for 1 hour at 60000 x g and then loaded on a 5 mL Histrap FF column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Cell Biology 2023Quote: ... was applied to a previously equilibrated HiTrap Chelating HP 5 ml column charged with 50 mM CoCl2 and coupled to a GSTrap HP 1 ml column (both GE Healthcare). Buffer A was used for equilibration ...
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bioRxiv - Molecular Biology 2023Quote: ... 70 μL of each sample at 1 mg/mL was fractionated in GF150 buffer using a Superdex 200 5/150 analytical gel filtration column (GE Healthcare) run at 0.1 mL/min on an Agilent 1200 series LC system collecting UV at 280 nm before then passing through the light scattering and refractive index detectors in a standard SEC MALS format ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Neuroscience 2020Quote: ... pGEX-4T-2 (GE Healthcare), pGIPZ control shRNA ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
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bioRxiv - Plant Biology 2020Quote: ... pGEX6p-2 (GE healthcare, IL) and pBE2113-GFP (Yoshida et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare), 0.5% penicillin-streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... images were obtained every 5 min for 4 h using a 20X objective (0.75 NA, PlanApo) on a DeltaVision microscope (GE Healthcare) equipped with a CoolSNAP HQCCD camera (Photometrics ...
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bioRxiv - Cell Biology 2020Quote: ... 5% glycerol (w/v, 0.1% Triton X-100) and mixed with 50 µl Glutathione Sepharose 4 Fast Flow (GE Healthcare) slurry ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Microbiology 2021Quote: ... The solution was centrifuged for 30 min at 38,000 × g and 4°C to remove precipitates and applied onto a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB3 ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared by two rounds of 20 min centrifugation at 45,000 x g at 4°C and loaded onto a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cooled down on ice and ultracentrifugated at 160,000xg for 1hr at 4°C and loaded onto a 5 mL Protein A (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Biophysics 2020Quote: ... the soluble fraction was isolated by centrifugation at 16,000 g for 1h at 4 °C and then loaded onto a 5 mL HisTrap HP column (GE Healthcare) previously equilibrated in the lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins were collected after a 5 min centrifuge at 17,000 g at 4 ℃ and immobilised on Glutathione-coated sepharose beads (GE Healthcare). For pull down with 35S-radiolabelled preoteins ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation (15,000 g for 40 min at 4 °C) and incubated with 5 mL glutathione Sepharose 4B (GE Healthcare) for 1.5 hr at 4 °C with gentle shaking ...
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bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified (16,000 x g for 45 min at 4 °C) before being loaded onto a 5 mL HisTrap column (GE Healthcare). This was followed by one column volume (c.v. ...
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bioRxiv - Molecular Biology 2023Quote: ... centrifuged at 16,000 g for 20 min at 4°C and then injected into Superose 6 5/150 increase column (GE Healthcare) pre-equilibrated with analytical size-exclusion chromatography buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5-ml column at 4° C (GE Healthcare). The column was washed with 10 vol ...
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bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2022Quote: ... Images were taken at 2 second intervals for 1 minute and deconvoluted with Applied Precision softWorx imaging software (GE Healthcare). The microtubule comet tracking and quantification was performed with the MTrackJ plugin in ImageJ/Fiji (Meijering et al. ...
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bioRxiv - Biochemistry 2022Quote: The interaction of N proteins and 14-3-3γ was analyzed using a Superdex 200 Increase 5/150 column (GE Healthcare) operated at a 0.45 ml/min flow rate on a Prostar 335 chromatographic system (Varian ...
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bioRxiv - Cancer Biology 2021Quote: ... Stained plates were washed 3 times in dH2O for 5 minutes at room temperature and air dried before imaging using the Imagescanner III (GE Healthcare). Growth was determined by percentage area of the plate covered by cells using ImageJ v1.52a ...
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bioRxiv - Plant Biology 2020Quote: ... Each Z-section image is based on 15 images generated from 3 different angles and 5 different SIM patterns and reconstructed using SOFTWORX software (Applied Precision).
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bioRxiv - Cell Biology 2020Quote: ... membranes were exposed for 10-30 s (in the case of GFP-Plk4) and for 3–5 min (in the case of actin) on a Amersham Imager 680 (GE Healthcare) and images were acquired with its CCD camera.
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bioRxiv - Genetics 2021Quote: ... The membrane was washed 3 × 5 min in PBST and then visualized by Amersham ECL Prime Western Blot Detection Reagent (GE Healthcare). Images were taken by Fluorescent Image Analyzer LAS-3000 (FujiFilm ...
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bioRxiv - Molecular Biology 2020Quote: ... and RNA primer 5’-rCrArGrUrGrCrUrArUrGrUr GrArGrArUrUrArArGrUrUrArU-3’ were prepared by solid phase synthesis on an ÄKTA oligopilot plus 10 (GE Healthcare). RNAs were cleaved from the solid support by treating with 4 mL of a 1:1 mixture of 28% wt NH3/H2O solution and 33% wt CH3NH2/EtOH solution at 55°C for 30 minutes ...