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Citations for GE Life Sciences :
201 - 250 of 3622 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Peak fractions were diluted with 2 volumes of buffer H and loaded onto a 1-mL SP HP column (GE Healthcare) and washed with buffer C (50 mM HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of freshly purified protein (concentration 1-2 mg/ml) was injected on a Superdex 75 300/10 GL column (GE Healthcare) at a flow rate of 0.5 ml/min ...
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bioRxiv - Immunology 2022Quote: ... mononuclear cells were isolated by density gradient centrifugation of PBS-diluted buffy coat/blood (1:2) over Ficoll-Paque Plus (GE Healthcare). The PBMC layer was carefully removed and washed 3 times with PBS.
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bioRxiv - Cancer Biology 2022Quote: ... Blood was diluted 1:2 with 1× phosphate-buffered saline (PBS) and transferred to a 50 ml falcon tube with Ficoll-Paque PLUS (GE Healthcare) at a 1:2 ratio ...
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bioRxiv - Biochemistry 2021Quote: ... purified OxlT was mixed with purified 20D033-Fv at a 1:2 molar ratio at 4 °C overnight and purified using Superdex200 Increase 10/300 GL (GE healthcare) in 20 mM MES-KOH ...
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Biochemistry 2022Quote: ... and passed through two subsequent gel filtration steps using a: 1) Hiload 16/60 Superdex 75 column (Cytiva #28989333) and 2) and Sephacryl S-300 column (GE Healthcare). Buffers used for this sizing step and subsequent labeling reactions were followed as for full-length proteins ...
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bioRxiv - Cell Biology 2023Quote: ... were used at a dilution of 1:10,000 and the HRP-linked sheep F(ab’)2 fragment to mouse IgG (GE Healthcare, NA9310) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a dilution of 1:10,000 and the HRP-linked donkey F(ab’)2 fragment to rabbit IgG (GE Healthcare, NA9340) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... both extracts (Triton and SDS) were diluted 2:1 in 50 nM Tris-HCl and incubated with 50 µl Streptavidin Sepharose (GE Healthcare) overnight at 4°C while rotating ...
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bioRxiv - Molecular Biology 2024Quote: An appropriate amount (0.5 – 2 µg) of total RNA was separated in 1% MOPS-formaldehyde agarose gels and transferred to Hybond-N + membranes (GE Healthcare). Membranes were exposed to either randomly [32P]-labelled dsDNA probes ...
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bioRxiv - Microbiology 2022Quote: ... antibodies diluted 1:500 in 1 U/mL DNAse I in Tris Buffered Saline containing 1% BSA (GE Healthcare). Three 5-minute washes with block (1% BSA with 0.1% Tween-20 ...
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bioRxiv - Microbiology 2023Quote: ... 1 strip of 1 x 10 cm Whatman Grade 1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA) was added to a test tube containing 5mL of M63 media ...
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bioRxiv - Genomics 2021Quote: ... 2 mM MgCl2) and then loaded onto two tandem 5 mL heparin HP pre-packed columns (GE Healthcare). Sen1 was eluted using a linear gradient from 20% to 100% of buffer B2 containing 50 mM HEPES-NaOH ...
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bioRxiv - Biophysics 2024Quote: ... a 2-elution step Ni2+-affinity chromatography was carried out using a Histrap HP 5 ml (GE Healthcare) on an FPLC apparatus (ÄKTA Pure ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mm x 1 mm x 1 mm) and FDG-PET data acquisitions were performed simultaneously on a hybrid 3 T SIGNA™ PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the Service of Nuclear Medicine (CUB Hôpital Erasme ...
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bioRxiv - Neuroscience 2021Quote: ... Then 50 µl of pre-washed (3×5 minutes in homogenising buffer) and equilibrated beads (Protein G Sepharose 4 Fast Flow, GE Healthcare, Sweden) were added and the sample mix was incubated overnight with gentle agitation ...
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bioRxiv - Neuroscience 2020Quote: The blots were subsequently washed 3 x 5 min in TBST 1X and developed with the Prime Western Blotting detection Reagent (GE Healthcare Amersham) using the LAS3000 Bioimager (Fujifilm ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated with GST or GST-tagged angulin-1 cytoplasmic region (409-575 aa or 409-570 aa) for 2-3 h at 4°C and further incubated with Glutathione Sepharose 4B beads (GE Healthcare) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Dynamic PET/CT imaging (2-3h) with arterial blood sampling was performed on Monkey 3 and Monkey 4 using a Discovery MI (GE Healthcare). Each animal had two baseline scans which were separated by one month for Monkey 3 and by one year for Monkey 4 ...
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bioRxiv - Neuroscience 2022Quote: ... The blots were visualised with the BioRad ChemiDoc XRS+ (Cohort 2) or the Amersham 6000 Gel Imager (GE Healthcare, Cohort 3), and quantified with Image Lab Software (BioRad) ...
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bioRxiv - Biophysics 2022Quote: ... 250 μL protein at a concentration of 2-3 mg/mL was injected on a Superdex200 10/300 increase column (GE Healthcare) equilibrated with 20 mM Tris pH 7 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were affinity purified against DLI-1(1-368) coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... The supernatant was then vacuum filtered and loaded at 1 mL/min onto a HiTrap Q HP column (5 mL, GE Healthcare Life Sciences) pre-equilibrated with buffer D [10 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... 45 μl of the protein at 4.43 mg ml−1 (70.4 µM) was injected in SEC column S200i 5/150 (GE Life Sciences, Velizy-Villacoublay, France) with a buffer containing 20mM Hepes pH 7,5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: we incubated samples for 10 min with Buffer A (1 M GA, 80% ACN, 5% TFA) followed by incubation with the TiO2 Mag Sepharose (GE Healthcare Life Sciences) for 30 min in an Eppendorf Thermomixer at 800 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted soluble fraction was then loaded at 1 mL/min in Buffer A onto a 5 mL HiTrap SPFF column (GE Healthcare Life Sciences) and eluted at 2 mL/min with a 70 mL gradient to 100% Buffer B ...
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bioRxiv - Plant Biology 2020Quote: ... MKK7 ORF was PCR amplified from Arabidopsis genomic DNA using primers MKK7-F and MKK7-R1 (Table 2) and cloned into pGEX-5X-1 (GE Healthcare, USA) into BamHI-EcoRI restriction sites ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions of 2 ml were collected at a flow rate of 1 ml/min on an ÄKTA prime Plus system (GE Healthcare Biosciences) equilibrated with buffer F ...
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bioRxiv - Microbiology 2020Quote: ... An additional affinity chromatography step was therefore included directly before gel filtration using 2 × 5 mL HisTraps (GE Healthcare). As both the MBP and TEV proteins incorporate a hexahistidine tag ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were developed using 1:1 Amersham ECL western blotting reagent (GE Healthcare) to SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... a 1:1 mixture of 50 mg/mL Sera-Mag SpeedBeads (GE Healthcare) and 50 mg/mL Sera-Mag SpeedBeads (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-FBH1[1-250] in the pGEX6P-1 vector (Amersham/GE Healthcare/Cytiva) was introduced into BL21:DE3 Rosetta cells (Novagen) ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... installed prone on the MRI bed (Fig 1) and anaesthesia was immediately switched to 1.5-2% isoflurane (28) in medical air through a respirator (Aestiva, GE Healthcare, Datex-Ohmeda, USA). The respirator allowed continuous control of respiration rates ...
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bioRxiv - Biochemistry 2022Quote: ... pre-equilibrated with buffer B (buffer A supplemented with 1 mM DTT and 2 mM EDTA) connected to an AKTA FPLC system (GE Healthcare Life Sciences). Fractions corresponding to the elution peaks were analyzed by electrophoresis to assess the presence of MreB ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT for further purification by anion exchange on a Mono Q 5/50 GL column (GE Healthcare) with a gradient from 80 mM to 1 M NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the resulting supernatant was passed through either a 0.45 or 0.8 μm pore size filter and loaded onto a 5 mL Ni+2-NTA HisTrap HP column (GE Healthcare) pre-equilibrated with binding buffer (20 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Cell Biology 2022Quote: ... Time-lapse images (5-10 z-sections, 0.5–2 μm separation) were deconvolved using SoftWoRx software (Applied Precision, GE Healthcare) and processed with FIJI/ImageJ ...
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bioRxiv - Molecular Biology 2023Quote: ... Analysis was done by western blotting using the primary antibodies directed against H3K36me3 (ab9050, 1:2000) or H3K36me2 (ab9049, 1:2500) and as secondary antibody the anti-rabbit HRP (Na934v, GE Healthcare, 1:5000). The signal was detected by chemiluminescence after the addition of Pierce™ ECL Western Blotting substrate.
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and incubated overnight with primary antibodies (aFLAG 1:2,500, aHSP90 1:50,000, GE Healthcare) containing 0.01% Sodium azide (Sigma Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 then incubated with 1:50 (w/w) PreScission Protease (GE Healthcare) for 18 hrs at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...