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Citations for GE Life Sciences :
2401 - 2450 of 4276 citations for Recombinant Human Pro NGF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Separation of the labeled protein from residual dye was carried out using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected with enhanced chemiluminescence (ECL) detection reagents (RPN2236 or RPN2235, Amersham Biosciences, GE Healthcare,) and ChemiDoc™ XRS+ System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were separated by SDS-PAGE and transferred onto 0.45μm pore Protran nitrocellulose membrane (GE Healthcare) using semi-dry transfer apparatus (Hoeffer) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein for crystallization was further purified by size-exclusion chromatography on a Superdex 75 (GE Healthcare) with gel filtration buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... Then tag-cleaved proteins was purified with ion exchange chromatography (HiTrap SP FF column (GE Healthcare). Target proteins in the HiTrap SP FF column were eluted by a linear gradient of NaCl and further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare) ...
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Molecular basis for the interactions of eIF2β with eIF5, eIF2B, and 5MP1 and their regulation by CK2bioRxiv - Biochemistry 2024Quote: ... Fractions containing the target protein were pooled and loaded onto a Superdex S75 column (GE Healthcare) equilibrated in buffer containing 150 mM KCl ...
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bioRxiv - Microbiology 2024Quote: ... The protein extract was precleared by incubation with nProtein A-Sepharose 4 Fast Flow (GE Healthcare) for 30 min and then incubated with 20 uL of rabbit anti-Myc antibody (ab9106 ...
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bioRxiv - Microbiology 2024Quote: ... The proteins separated on the gels were transferred to nitrocellulose membranes (Amershan Protan membranes, GE Healthcare) using transfer buffer (0.192 M Glycine ...
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bioRxiv - Biochemistry 2019Quote: The interaction of the recombinant heterodimeric (‘monomer’) and homodimeric (‘dimer’) human G6b-B extracellular domain with different ligands was quantified using a BIAcoreTM 8K instrument (GE Healthcare, Little Chalfont, UK). Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... or monocytes were isolated from buffy coat of healthy human donors (Biological Specialty Corp) by density centrifugation in Ficoll®-Paque PLUS (GE Healthcare Life Sciences). Monocytes were enriched either through positive selection using CD14 MicroBeads (Miltenyi Biotech ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgM were captured by affinity chromatography using HiTrap Protein G High Performance column (GE Healthcare) and CaptureSelect IgM Affinity Matrix (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-ROC WT and GST-ROC K1347A mutant protein bound to Glutathione Sepharose 4B beads (GE Healthcare) were washed twice with Buffer A (20 mm Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein signal was detected using chemiluminescence with the ECL Prime Western Blotting Detection Reagent™ (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... 12 % and 15 % gels followed by protein transfer onto nitrocellulose membranes (GE Healthcare Life Sciences, 0.45 µm). For better visibility of endogenous HA-hATG8s membranes were boiled for 5 min in PBS after protein transfer ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were pre-cleared with a 20 μl bed volume of protein-A sepharose beads (GE Healthcare) by end-over-end rotation for 1 hr at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Labeled protein was further purified on a Superdex 200 increase 10/300 gel filtration column (GE Healthcare) equilibrated in buffer B + 100 mM potassium acetate (KOAc) ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein bands were visualized using an Amersham ECL Advance Western Blotting Detection Kit (GE Healthcare, Germany).
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bioRxiv - Microbiology 2020Quote: ... ECL (Amersham) chemiluminescence substrates were used for protein detection using an ImageQuant LAS 4000 system (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The translated proteins were resolved using SDS-PAGE and analyzed by phosphorimager analysis (Typhoon, GE life sciences).
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bioRxiv - Molecular Biology 2021Quote: ... The fusion proteins (GST-OsbZIP86 and MBP-OsSAPK10) were purified using Glutathione Sepharose 4B (GE Healthcare, USA) and Amylose Magnetic Beads (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Excess reducing agents were removed by running the protein solution through a PD-10 column (GE Healthcare) equilibrated with 20 mM HEPES ...
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bioRxiv - Developmental Biology 2021Quote: ... Separated proteins were then wet transferred to a 0.45 μm nitrocellulose membrane (GE Healthcare Life Science, 10600003) in Towbin buffer (25mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... Bands corresponding to various proteins were detected by corresponding antibodies using ImageQuant LAS 4000 (GE Healthcare, Tokyo) and analyzed using ImageQuant software ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing 2F2 or 2E10.E9 IgG was purified via Protein G affinity chromatography (GE Healthcare) and size-exclusion chromatography (Superose 6 Increase 10/300 GL ...
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bioRxiv - Molecular Biology 2021Quote: ... The immobilized proteins were eluted and further purified by a mono Q 5/5 column (GE Healthcare) and a Superdex75 10/300 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The deglycosylated proteins were then purified by batch affinity chromatography on Glutathione Sepharose 4B resin (GE Healthcare) followed by SEC ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 μL lysate (2 mg protein) was injected through a 0.75mm loop (GE Healthcare 18-1112-53). Run parameters were 0.5 mL/min and 1.5MPa for 1 column volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μL of protein was injected onto a Superdex 75 increase 10/300 GL column (GE Healthcare) pre-equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were purified by Ni-NTA IMAC using Ni-Sepharose 6 Fast Flow resin (GE Healthcare). The wash buffer consisted of 50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin was precleared for 1 hour at 4 °C with blocked protein A Sepharose beads (GE Healthcare, Eindhoven ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with 0.5 ml protein-A-sepharose (nProtein A Sepharose 4 Fast flow, GE healthcare #1705280) at 4 °C for 24 hr ...
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bioRxiv - Biophysics 2022Quote: ... ion exchange chromatography for initial protein purification was performed on a 1ml HiTrap SP FF (GE healthcare) and a 24ml Superdex 75 10 300 GL (GE healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... was used to visualize protein bands using an ImageQuant LAS4000 imager (GE Healthcare #28955810, Chicago, Illinois, USA).
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected from selected hybridoma clones and purified through a protein G-sepharose column (GE Healthcare). mAbs were isotyped with Pierce rapid isotyping kit (Cat#26178) ...
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bioRxiv - Biochemistry 2020Quote: ... Purified protein was transferred to appropriate buffers by size exclusion chromatography (Superdex 75 10/300, GE Healthcare) and stored at 4°C.
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bioRxiv - Microbiology 2020Quote: ... The buffer was filtered (0.2 µm filters) prior to protein separation on an Äkta Purifier (GE Healthcare). The fractions containing RNA polymerase were pooled and dialyzed against RNA polymerase storage buffer (50 mM Tris–HCl ...
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bioRxiv - Microbiology 2020Quote: ... HR1-L6-HR2 protein was concentrated and gel-filtered on a 10/300 Superdex 75 (GE Healthcare) column ...
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bioRxiv - Molecular Biology 2019Quote: ... the reaction was added to 15 µL (30 µL 50% slurry) Protein A:Protein G beads (GE Healthcare) pre-coupled with antibodies ...
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bioRxiv - Microbiology 2019Quote: Fifty micrograms of protein extract from each sample were labeled with Cy3- or Cy5 Dye (GE Healthcare). Labeling was performed reciprocally so that each sample was separately labeled with Cy3 and Cy5 to account for any preferential protein labeling by the CyDyes ...
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bioRxiv - Microbiology 2019Quote: Isolated proteins for each fraction and genotype were then precipitated with 2D Clean-Up kit (GE Healthcare), and dissolved in TS buffer (7M urea ...
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bioRxiv - Biochemistry 2019Quote: ... and the cleaved protein was further purified using FPLC first with cation exchange (Source-S, GE Healthcare), followed by gel filtration (Superdex 75 ...
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bioRxiv - Zoology 2019Quote: ... Wet-transfer of the proteins from the gel to a polyvinylidene difluoride transfer membrane (GE Healthcare, UK) was according to [40] with minor modifications ...
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bioRxiv - Biochemistry 2019Quote: Protein samples were injected onto a Superdex S200 or S75 16/60 size exclusion column (GE Healthcare), which was run at 1 ml.min-1 in 25 mM HEPES pH 7.0 ...
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bioRxiv - Genomics 2020Quote: ... Eluted protein samples were further purified by gel filtration using Superose 6 10/300 column (GE Healthcare). These proteins were buffer-exchanged to storage buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by gel filtration chromatography on Superdex 75 16/600 column (GE Healthcare) in 50 mM Sodium Phosphate ...
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bioRxiv - Microbiology 2019Quote: ... The protein was purified from the soluble fraction using HisTrap HP and Superdex 75 columns (GE Healthcare) in 50 mM HEPES (pH 7.4) ...
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bioRxiv - Immunology 2019Quote: ... followed by the addition of 30 μl of a 50% slurry of protein G beads (GE Healthcare), and incubation overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted proteins were pooled and concentrated for size exclusion chromatography (16/600 Superdex 75pg GE healthcare). The Tau concentration was measured using BCA method.