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Citations for GE Life Sciences :
2401 - 2450 of 2611 citations for Pituitary Tumor Transforming 1 PTTG1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... were used at a dilution of 1:10,000 and the HRP-linked sheep F(ab’)2 fragment to mouse IgG (GE Healthcare, NA9310) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... 70 μL of each sample at 1 mg/mL was fractionated in GF150 buffer using a Superdex 200 5/150 analytical gel filtration column (GE Healthcare) run at 0.1 mL/min on an Agilent 1200 series LC system collecting UV at 280 nm before then passing through the light scattering and refractive index detectors in a standard SEC MALS format ...
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bioRxiv - Biochemistry 2023Quote: ... of GcapOCP2 or CrinOCP2 holoform samples (1 mg/ml in 100 μl) was carried out on a Superdex 200 Increase 10/300 column (GE Healthcare) connected to a tandem of consecutive detectors Prostar 335 (Varian ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Biochemistry 2023Quote: SUV420H1-H2A.Z nucleosome complex was assembled in a ratio of 3:1 and was purified on HiLoad Superdex 200 10/300 (GE Healthcare) size-exclusion liquid chromatography column (Buffer ...
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bioRxiv - Bioengineering 2023Quote: ... A Biosep 10L acoustic filter (Applikon Inc., Foster City, CA) related to the bioreactor thanks to two peristaltic pumps (P-1, GE Healthcare), one for medium recirculation and one for sample withdrawal (Henry et al. ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a dilution of 1:10,000 and the HRP-linked donkey F(ab’)2 fragment to rabbit IgG (GE Healthcare, NA9340) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... by capillarity and membranes were hybridized with a 32P-labelled oligonucleotide probe (23S.1, 5’- GATTGCTCGAATGCCCAAAG-3’) using Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to Phosphorimaging screens and analysed using a Typhoon Tryo (GE Healthcare).
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bioRxiv - Biophysics 2023Quote: ... The fixed cells were rinsed three times in warm PBS and incubated for 30 min with a blocking solution containing 1% BSA (GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was stained with DAPI (1 µg/mL) for 5 minutes at room temperature and cells imaged with a DeltaVision Core system (Applied Precision).
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Biochemistry 2023Quote: ... Insoluble fractions were removed by centrifugation at 20000 g during 30 min at 4°C and the supernatant was loaded on a 1 mL HisTrapTM HP column (GE Healthcare) connected to a NGC chromatography system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μl of each sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), and the dry membrane was incubated in NRP solution (0.01 g/ml KMnO4 in ddH2O ...
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bioRxiv - Biophysics 2023Quote: ... The solution was concentrated to ∼1 ml and went through gel filtration on Superdex 75 10/300 increase column (GE Healthcare) equilibrated with a buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Final purification and concentration of the untagged DAP5 was performed using a 1 ml HiTrap heparin HP column from GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... Time-lapse imaging of one-cell embryos expressing CYB-1::mNG or mNG::CYB-3 was performed on a widefield deconvolution microscope (DeltaVision Elite; Applied Precision) equipped with a charge-coupled device camera (pco.edge 5.5 sCMOS ...
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bioRxiv - Molecular Biology 2023Quote: ... As done in our previous experiments (Cattaneo et al., 2022) stimuli aliquots were spread on grade 1 – 20 mm circles filter paper (GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2023Quote: ... Vectors suitable for expression and purification of His6-and/or glutathione S-transferase (GST)-tagged recombinant proteins in Escherichia coli were either cloned by Twist Biosciences into the pET28a+ backbone (EMD Biosciences) or cloned in-house into the pGex6p.1 backbone (GE Healthcare). When needed ...
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bioRxiv - Molecular Biology 2023Quote: ... the TEV-cleaved proteins were purified in two steps: 1) IEX on 5 mL HiTrap Q HP (GE Healthcare Life Sciences) using as buffer A ...
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bioRxiv - Microbiology 2023Quote: ... with a 10 kDa cut-off and loaded onto a Superdex 200 pg HiLoad 16/600 (GE Healthcare, 1 mL/min). Sample purity was assessed by 12% SDS-PAGE analysis and sample concentration was determined by spectrophotometry using a theoretical molar extinction coefficient at 280 nm of 20 ...
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bioRxiv - Cell Biology 2023Quote: ... or phosphate buffered saline containing 1% human serum albumin (Grifols Biologicals, Los Angeles, CA, USA) using either a PD10 desalting column (GE Healthcare) or a Vivaflow 50 cassette (Sartorius ...
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bioRxiv - Systems Biology 2023Quote: ... Isolated cells were washed once in PBS (2mM EDTA, 1% FBS) and lymphocytes were enriched on a 40-67% Percoll gradient (GE Healthcare).
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bioRxiv - Immunology 2024Quote: Tested antibody (5 μg/mL for NTD and 1 μg/mL for spike) was captured on protein A sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified SARS-CoV-2 mutant spike or NTDs ...
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bioRxiv - Physiology 2024Quote: ... Membranes were washed twice with TBS containing 0.1% Tween-20 and once with PBS containing 0.1% Tween-20 and then incubated with goat anti-mouse or goat anti-rabbit horseradish peroxidase (HRP)-conjugated IgG (GE Healthcare) diluted 1:10000 or 1:5000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The Triton X-100 was then removed by either (i) binding to a HisTrap 1 mL column (GE Healthcare, Chicago, USA) or HIS-Select Nickel Affinity Gel in a gravity-flow column (Merck ...
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bioRxiv - Bioengineering 2024Quote: The RSVF RSVF-multi-1 immunogen and motavizumab Fab were purified by size exclusion chromatography using a Superdex200 26 600 (GE Healthcare) equilibrated in 10 mM HEPES pH 7.5 and 150 mM NaCl and concentrated to ∼20 mg/ml (Amicon Ultra-15 ...
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bioRxiv - Biophysics 2024Quote: ... TEV-cleaved protein was then diluted 1:500 in KBT and then loaded onto a HiTrap Q HP column (GE Healthcare) for ion exchange chromatography (IEC ...
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bioRxiv - Biochemistry 2024Quote: ... 2 mM TCEP) and incubated for 1 hour at 4°C before loading on Superose 6 5/150 column (GE Healthcare) equilibrated with the same buffer on an AKTA-micro system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... This lysate was applied onto a 1 mL HisCap Smart 6FF column (Smart-Life Sciences) using an Akta Purifier 10 chromatograph (GE Healthcare). Subsequent washing steps were carried out with mix of buffer A and buffer B (buffer A + 500 mM imidazole) ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was discarded and the pellet was resuspended in 1 mL of Percoll® (GE Healthcare Bio-Science AB, Sweden). After strong agitation followed by centrifugation ...
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bioRxiv - Immunology 2024Quote: ... Complexes were generated by incubating Fab and antigen overnight at a 50:1 ratio and purified through size exclusion chromatography with a Superdex 200 increase 10/300 column (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... both extracts (Triton and SDS) were diluted 2:1 in 50 nM Tris-HCl and incubated with 50 µl Streptavidin Sepharose (GE Healthcare) overnight at 4°C while rotating ...
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bioRxiv - Plant Biology 2020Quote: ... A peroxidase-conjugated secondary antibody was used at a dilution of 1:5000 and immunoreactive bands were visualized with ECL Western Blotting Detection Reagents (GE Healthcare, UK).
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences). Method for EMSA was modified from a previously published protocol (30) ...
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bioRxiv - Physiology 2022Quote: ... at a dilution of 1:2000 was done for 1 hour at RT in blocking buffer and detection was done using ECL detection kit (RPN2235, GE Healthcare, USA). Images were taken by the ImageQuant LAS500 (GE healthcare ...
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bioRxiv - Biophysics 2022Quote: ... and analysed by electrophoresis in a blue native gel containing 4 - 16% gradient of acrylamide (29:1 Acrylamide:Bis) using the SE 600 Vertical electrophoresis system (GE Healthcare Life Sciences). The gels were run at 4 °C at constant 200 V for 2 h followed by 6 h at 600 V with max ...
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bioRxiv - Plant Biology 2019Quote: ... containing 0.3 mmol L-1 NH4HCOO (adjusted to pH 3.5 with formic acid) delivered by a Pharmacia 2248 HPLC pump (GE Healthcare, Munich, Germany) were added just after the column via a mixing tee valve ...
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bioRxiv - Biochemistry 2019Quote: The RRM domain of RBFOX2 (amino acids 100-194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28-9546-48) downstream of a GST-SBP tandem affinity tag ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... to a polyvinylidene difluoride membrane and blocked in TBST buffer (TBS with 0.1% (v/v) Tween-20) supplemented with 1% ECL Advance Blocking Agent (GE Healthcare, Waukesha, WI) for 40 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... in the presence of 50 µCi of 32P-α-UTP and 1 mM cold UTP and purified on a Sephadex G25 column (GE Healthcare). Hybridization was performed in the modified Church Buffer at 65°C and the membrane was washed two times 5 min in 2X SSC ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Biochemistry 2019Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... To prevent non-specific binding the surfaces were passivated by injections of 0.2 mg/ml BSA with 1 mg/ml NSB (GE Healthcare Life Sciences) for 120 s prior to a 120 s injection at 30 µl/min of either TtBac-WT ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM EDTA [pH 7.5]) for 2hrs at room temperature and gels were imaged on ImageQuant LAS4010 imager (GE Healthcare Life Sciences).