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Citations for GE Life Sciences :
2401 - 2450 of 4116 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Protein bands were visualized with ECL™ Prime Western Blotting detection reagents (GE Healthcare Life Sciences, RPN2236) and the pictures were taken with Bio-Rad ChemiDoc™ MP Imaging System.
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bioRxiv - Cell Biology 2022Quote: ... and then combined with 60 μl of washed Protein A–Sepharose beads (17-0780-01, GE Healthcare) for 1 hour on a rotator at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing GST-protein were pooled and loaded to a 6 mL Resource S column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Genetics 2022Quote: ... and proteins were transferred onto a membrane (Nitrocellulose Blotting Membrane 10600002; GE Healthcare Life Sciences; PA; USA). Primary antibodies were incubated overnight at 4°C and included mouse anti-3OH-K (1:1000 ...
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bioRxiv - Genetics 2022Quote: ... Separated proteins were then wet transferred to a 0.45 μm nitrocellulose membrane (GE Healthcare Life Science, 10600003) in Towbin buffer (25mM Trizma base ...
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bioRxiv - Immunology 2022Quote: ... MBs were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare, Chicago, IL) and eluted with 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Imidazole was removed by passing the eluted protein fraction over a desalting NAPTM-25 column (GE Healthcare) equilibrated with buffer A and eluted with 2.5 ml of the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... supernatant containing VSG protein was loaded onto an anion-exchange column (Q-Sepharose Fast-Flow, GE Healthcare), which had been equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated and loaded onto a Superdex 200 16/60 size exclusion column (GE Healthcare) pre-equilibrated at 4 °C in SEC buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant protein GST-LSH10 was purified using Glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... The proteins were visualized by a chemiluminescence method (Amersham Imager 600, GE healthcare Life Sciences, Buckinghamshire, UK). Protein signals were quantified using ImageJ software (National Institute of Health ...
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bioRxiv - Plant Biology 2023Quote: ... Two mg of proteins in buffer were mixed with 50ul 7-Methyl-GTP-Sepharose 4B (GE Healthcare) previously equilibrated with extraction buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The cleaved proteins were separated by a 16/600 Superdex75 gel filtration column (GE Healthcare, Chicago, lL), followed by depleting the peak of lgMPZ peak or residual MBP by by applying to amylose resin ...
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bioRxiv - Biophysics 2022Quote: ... The dialyzed protein solution (∼50 μM) was then loaded into the sample cell in iTC200 (GE Healthcare) after the removal of insoluble materials by centrifugation (10,000×g ...
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bioRxiv - Cell Biology 2022Quote: ... cleared protein was subjected to size-exclusion chromatography using a HiLoad 16/600 Superdex column (GE Healthcare) in lysis buffer ...
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bioRxiv - Microbiology 2022Quote: Total IgG was purified from the final bleed rabbit sera using Protein G Gravitrap (GE Healthcare, Cytiva). The sera were diluted as 1:5 in binding buffer (20 mM Sod ...
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bioRxiv - Biochemistry 2024Quote: ... 200 µL of protein was injected into the Superdex 75 or Superdex200 size exclusion column (GE Healthcare) (volume less than 900 µL for preparative runs) ...
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bioRxiv - Immunology 2023Quote: ... The biotinylated proteins were further purified by anion-exchange chromatography using a Mono Q column (GE Healthcare) and stored in 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins of interest were detected using Chemiluminescence by adding Amersham Enhanced chemiluminescence (ECL) detection reagent (GE healthcare). For quantification of Yops-secretion ...
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bioRxiv - Microbiology 2024Quote: Recombinant WT and mutant IN proteins were analyzed using Superdex 200 10/300 GL column (GE Healthcare) with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein was subsequently purified using a Superdex75 gel-filtration column (GE Healthcare, Chicago, IL, USA). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... and guinea pig anti-Rad21 and guinea pig anti-Pds5b coupled to protein A sepharose (GE Healthcare). For non-covalent coupling of antibodies to beads ...
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bioRxiv - Plant Biology 2023Quote: ... The protein was produced in Escherichia coli strain Rosetta and purified with nickel Sepharose media (GE Healthcare). EMSA was performed according to the reference guide of the LightShift Chemiluminescent EMSA kit (Thermo Scientific 20148) ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.1LmM PMSF) and included 80 pmol of GST-fused protein immobilized on GSH beads (GE Healthcare), 1–8 µg of heparin sulfate ...
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bioRxiv - Molecular Biology 2024Quote: ... The recovered proteins after cleavage underwent size exclusion chromatography using a Superdex S75/300 column (GE Healthcare) in phosphate-based buffer [200 mM sodium phosphate pH 7.4 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified from soluble fractions of the bacterial cell lysates using Glutathione Sepharose beads (GE Healthcare). Proteins were dialyzed in 1x TBS overnight at 4°C overnight and protein quantified ...
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bioRxiv - Biochemistry 2023Quote: Proteins were analyzed on a Superdex 200 10/300 GL column from GE healthcare (Chicago, IL, USA) equilibrated using a mobile phase containing 150 mM KCl and 50 mM Tris-HCl pH 7.6 run at 0.4 ml/min ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were separated on a 12% SDS-PAGE gel and transferred to a nitrocellulose membrane (GE Healthcare), which was then inucbated over-night with primary antibodies followed by a 1-hour incubation with fluorescent secondary antibodies (Table 1) ...
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bioRxiv - Biophysics 2024Quote: ... the protein sample was passed through a Hi-Trap Q-Sepharose FF crude (GE Healthcare, Piscataway, NJ) column ...
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bioRxiv - Microbiology 2023Quote: Protein samples were separated on 10–15% polyacrylamide gels and transferred to Amersham nitrocellulose membranes (GE healthcare) by Trans-blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by SEC using a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated with Buffer B ...
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bioRxiv - Biochemistry 2022Quote: ... Protein purity was assessed by SDS-polyacrylamide gel electrophoresis (PhastGel Homogenous 20 pre-cast gels, GE Healthcare) and the enzyme concentration ([E] ...
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bioRxiv - Biochemistry 2022Quote: ... For crystallography proteins were purified further via size-exclusion chromatography (HiLoad 16/600 Superdex 200, GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were pre-incubated with 60 uL of protein A-Agarose beads (GE Healthcare, Chicago, USA) + Salmon Sperm DNA (Invitrogen #15632-011 ...
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bioRxiv - Microbiology 2022Quote: Proteins were purified by immobilized metal affinity chromatography (IMAC) using Ni-Sepharose excel resin (GE Healthcare, USA). The column was first washed with 5 column volumes of loading buffer (50 mM tris(hydroxymethyl)aminomethane (TRIS) ...
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bioRxiv - Microbiology 2022Quote: ... cell culture media were clarified by centrifugation and the IgG captured using Protein A resin (GE Healthcare). Proteins were eluted from the resin using citrate buffer pH 3 ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by nickel affinity chromatography using a 5 ml HisTrap HP column (GE Healthcare). The eluted proteins were dialyzed into 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... The pull-down of biotinylated proteins was performed using 100 µl StrAv Mag Sepharose™ (GE Healthcare) for each condition and incubated in a rotation wheel for 3h at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... and the eluted proteins were further purified via gel filtration chromatography with a superdex 200 (GE Healthcare) column ...
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bioRxiv - Cell Biology 2023Quote: ... wet electroblotting (100V for 1h) was used to transfer proteins onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). After blocking (blocking solution ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were immobilized by blotting them onto an Amersham Protran Premium nitrocellulose membrane (GE Healthcare Life Sciences) in cold 1 x Tris-Glycine ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted proteins were pooled and concentrated before separation on a Superdex 200 16/60 column (GE Healthcare) equilibrated in GF2 Buffer (50 mM Tris-HCl pH 7.5 ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then purified using a ResourceTM Q 6ml column (17-1179-01, GE Healthcare, Uppsala, Sweden) and a Superdex 200 Increase 10/300 column (28-9909-44 ...
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bioRxiv - Genetics 2023Quote: ... Protein containing fractions were further purified by size exclusion chromatography using Superdex 200 10/300 (GE Healthcare) gel-filtration column in SEC buffer (20 mM HEPEs pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The proteins were concentrated and gel-filtrated on a SuperdexTM 75 size exclusion chromatography column (GE Healthcare) equilibrated with stock buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Bioengineering 2023Quote: ... The target protein was purified by loading the supernatant onto a prepacked His SpinTrap column (GE Healthcare) and eluting with a buffer containing 20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples with indicated concentrations were loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... The cleaved protein was applied to a Superdex 200 Increase 10/300 GL column (GE Healthcare, USA) pre-equilibrated with gel filtration buffer (150 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... The filtered supernatant included NMnPP protein was applied to a nickel-nitrilotriacetic acid (Ni-NTA, GE Healthcare) affinity column pre-equilibrated with buffer A ...