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Citations for GE Life Sciences :
2401 - 2450 of 2686 citations for 8 4 Chlorophenylthio guanosine 3' 5' cyclic Monophosphate triethylammonium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The cell debris was pelleted by centrifugation and the supernatant was loaded onto a 5 ml HisTrap IMAC HP Nickel Sepharose column (GE Healthcare), using an ÄKTA Pure chromatography system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were precipitated the supernatant by addition of ammonium sulfate to 67% saturation and dissolved in buffer D and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer D ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 13,000 g for 60 minutes and the filtered supernatant was loaded onto a 5 mL Ni-NTA column (GE Healthcare, USA) equilibrated with binding buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG-eluted motor domains (wild-type + jordan) were bound to a strong anion exchanger (5/50 MonoQ GL; Cytiva) using a Purifier 10 chromatography system (GE Healthcare). The column was washed with MOPS (10) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleaved MBP tag and TEV protease were removed by anion exchange chromatography using a Mono Q 5/50GL (GE Healthcare) column pre-equilibrated with buffer-B ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% glycerol) to the 50 mM NaCl final concentration and loaded on equilibrated 5 mL HiTrap Heparin HP sepharose column (GE Healthcare). The protein was eluted with a linear 0-1 M NaCl gradient in buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... and fl-SNED1 (4.5 μM) were injected on Superdex 75 Increase 10-300 GL and Superdex 200 Increase 10/300 GL columns (GE Healthcare) respectively at a flow rate of 0.5 ml/min at 20°C ...
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bioRxiv - Biophysics 2021Quote: ... The pooled fractions were dialyzed against buffer A + 100 mM NaCl before loading onto a 5 mL Hitrap Heparin column (GE Healthcare) equilibrated with the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... it was passed through a 0.22 µm filter to remove potential precipitates and applied onto a 5 ml HisTrap HP column (GE Healthcare, USA) equilibrated with buffer BZ3 as described above ...
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bioRxiv - Molecular Biology 2022Quote: ... Undigested protein and TEV protease were removed by a second Ni-NTA step and flowthrough containing OCS was concentrated to 5 ml for subsequent gel filtration (Hiload 16/60 Superdex 200 pg, GE healthcare). Relevant fractions were pooled together ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein purification was performed on HisTrap FF crude 5 ml Ni-based column using ÄKTA Xpress System (Cytiva, former GE Healthcare). For that a wash buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was first purified by Immobilized Metal Affinity Chromatography (IMAC) by loading the clarified lysate onto a 5 mL Nickel column (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates from 8 L of yeast treated cells were then subjected to a 5 ml Nickel affinity purification using an AKTA purifier system (GE Healthcare) equilibrated in the urea buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The mTORC1 complex and free RAPTOR were separated by anion exchange chromatography on a 5 mL HiTrap Q column (GE Healthcare). The fractions containing mTORC1 complex and free Raptor were concentrated to 1.3 mg/mL and 0.5 mg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed and cleared lysate prepared as described and loaded onto a 5 mL Strep-Trap HP column (GE Healthcare), equilibrated with buffer D ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 or 10 μg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Biochemistry 2022Quote: ... soluble His-GST-APLFAD (or His-SUMO-APLFAD) was loaded on a 5 mL HisTrap FF column (GE Healthcare Life Sciences), pre-equilibrated in lysis buffer (50 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Neuroscience 2022Quote: ... DARPins after IMAC purification were analyzed at a concentration of 10 µM on a Superdex 75 5/150 GL column (GE Healthcare) using an Aekta Micro system (GE Healthcare ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... and equal amounts of protein were separated on 5–20% SDS‒PAGE gels (Wako) and transferred to Hybond-P membranes (GE Healthcare). The following primary antibodies and dilutions were utilized ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The urea concentration was set to 6 M and the sample was applied to a 5 mL HiTrap QFF anion exchange column (GE healthcare) and was eluted using a 30 CV long gradient from low salt buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to ∼2 µM for cryo-EM sample preparation.
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Neuroscience 2024Quote: Molecular masses of purified gephyrin and variants were detected by analytical size exclusion chromatography (SEC) using a Superose 6 increase 5/10 (GE Healthcare). Typically ...
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bioRxiv - Neuroscience 2024Quote: ... RNA (5 μg) was used to synthesize the first-strand complementary DNA (cDNA) using the First-Strand cDNA synthesis kit (GE Healthcare) with a pd(N)6 primer following the manufacturer’s indications ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell extracts were centrifuged to remove cell debris at 20,000 x g for 60 min prior to loading of a 5 mL HiTrap SP-FF column on an ÄKTA purifier FPLC (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Microbiology 2024Quote: ... fully complementary to the fluorescent strand to avoid it re-hybridizing to the quencher strand) and 5 mM ATP (GE Healthcare) in a 100 µL reaction solution containing 10 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... participants were asked to lay in the supine position for a minimum 5 minutes prior to full-body dual-energy x-ray absorptiometry (DXA) scan (Lunar Prodigy, GE Healthcare). Lean/soft tissue mass (also commonly termed as lean body mass ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V; HRP antirabbit NA934V, both GE Healthcare) and detected using chemiluminescence Clarity or Clarity Max ECL western blotting substrate (Biorad 1705061 or 1705062).
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bioRxiv - Neuroscience 2024Quote: ... the membranes are subjected to five more washes in TBS-T before being incubated in chemifluorescence substrate for a maximum of 5 minutes and scanned on either a Storm 860 Scanner (Amersham Biosciences) or Typhoon FLA 9000 Scanner (GE Healthcare) or being visualized in a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2024Quote: ... 60 μl of this mixture was added in the HUVEC side channel of the chip and max intensity projections were taken every 5 minutes for 30 minutes using the IN Cell Analyzer 6000 (GE Healthcare) microscope.
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) preequilibrated with the HiTrap binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The desalted solution containing protein of interest was applied to a HiTrap Q HP 5 mL column (GE Healthcare UK Ltd.) pre-equilibrated with the HiTrap binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was loaded onto a pre-washed/pre-equilibrated CM Sepharose HiTrap column (5 ml, GE Healthcare, Chicago, IL) on an Äkta explorer FPLC (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were fixed with 5% cold trichloroacetic acid and then dried onto chromatography paper (Whatman) and autoradiographed with Amerhsam Hyperfilm-MP (GE Healthcare). Gel images were scanned using a Typhoon phosphorimager (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was then filtered through 0.2 µm PES filter membrane and loaded onto a HisTrap HP column (5 ml, 1.6 x 2.5 cm, Ni Sepharose High-performance column, GE Healthcare, now Cytiva) pre-equilibrated with buffer A at 1 mL/min ...
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 2µM for cryo-EM sample preparation.
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bioRxiv - Cell Biology 2023Quote: ... was applied to a previously equilibrated HiTrap Chelating HP 5 ml column charged with 50 mM CoCl2 and coupled to a GSTrap HP 1 ml column (both GE Healthcare). Buffer A was used for equilibration ...
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bioRxiv - Biochemistry 2023Quote: ... The clear top phase obtained above a white band was collected and separated over a 5 ml HiTrap Q FF column (GE Healthcare) equilibrated with buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... A 2-fold molar excess of LF was mixed with 925 pmoles of hexon and incubated on ice for 5 min followed by SEC on Superose 6 increase 10/300 (GE Healthcare) equilibrated with buffer D ...
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bioRxiv - Cell Biology 2024Quote: ... The eluate was concentrated to a volume of 5 mL and loaded onto a size-exclusion-chromatography column (Superdex 16/60, GE Healthcare); HR3 LBD was eluted in a buffer of 150 mM NaCl ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was clarified using 0.45 µm filters before loading onto a nickel-Sepharose Fast Flow column (5 mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The column was washed with 40 mL of extraction buffer ...
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bioRxiv - Biochemistry 2022Quote: ... were solubilised in TK buffer supplemented with 6 M urea before loading onto a 5 ml HisTrap HP column (GE Healthcare). The column was first washed with TK + 6 M urea followed by TK + 6 M Urea + 50 mM imidazole ...
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bioRxiv - Molecular Biology 2022Quote: ... to a final concentration of 50 mM NaCl and loaded on an equilibrated 5 ml HiTrap Heparin HP Sepharose column (GE Healthcare). The protein was eluted using a linear 0–1 M NaCl gradient in buffer B ...
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bioRxiv - Biochemistry 2022Quote: ... injected (5 mL total) into HiLoad 16/600 Superdex 75pg (Cytiva) connected to an AKTA FPLC system (GE Healthcare Life Science). Gel filtration was performed using the same buffer to purify Nurr1-RXRα LBD heterodimer for analysis ...
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bioRxiv - Immunology 2022Quote: ... His-tagged Fabs were prepared by transient transfection of truncated heavy chain genes encoding a C-terminal 6x-His tag with a light chain expression vector and purified from supernatants using a 5 mL HisTrap colum (GE Healthcare) followed by SEC as described above.
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bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on two 5 mL-HiTrap heparin columns (GE Healthcare) at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were disrupted by sonication and the soluble part of the lysate was purified on a 5 mL-HiTrap heparin column (GE Healthcare) at 4 °C ...