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Citations for GE Life Sciences :
2351 - 2400 of 4331 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Protein samples were resolved by SDS-PAGE and transferred to Amersham Protran membranes (GE Healthcare) in a tankblot device (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 1 h of incubation with protein A–Sepharose CL-4B beads (GE Healthcare). Immunoprecipitates were washed three times in lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins separated by 10 % Tris-glycine SDS–PAGE were transferred to nitrocellulose membranes (GE Healthcare) and processed for immunoblotting using the ECL Prime kit (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... The protein solution was applied twice through a Concanavalin A Sepharose 4B column (GE Healthcare), equilibrated in 20 mM Tris ...
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bioRxiv - Plant Biology 2022Quote: ... The protein was then enriched to high purity using a Ni-Sepharose column (GE Healthcare) and stored at 4°C before use.
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bioRxiv - Molecular Biology 2022Quote: ... the untagged protein was further purified through 15Q anion exchange column (GE Healthcare Life Sciences) and Uniondex 200 pg 16/60 size-exclusion column (Union Biotech ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were separated on a Superdex 75 10/300 increase SEC column (GE Healthcare) with a flow rate of 0.5 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: ... the Red1 proteins were polished using gel filtration (Superdex 75 16/60) column (GE Healthcare) equilibrated with buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were transferred using the semi-dry method onto a nitrocellulose membrane (Whatman, GE Healthcare). The membrane was blocked in the blocking buffer (5% skimmed milk ...
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bioRxiv - Immunology 2022Quote: ... The protein was fractionated by size-exclusion chromatography (Superose 6 increase 5/150, GE Healthcare) with buffer F (20 mM HEPES at pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were detected with a chemiluminescent substrate according to the manufacturer’s instructions (GE Healthcare). The membranes were processed for chemiluminescence detection of actin (43 kDa ...
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bioRxiv - Cell Biology 2023Quote: ... Membrane total protein was visualized using an Amersham Imager 600 (AI600) (GE Healthcare Life Sciences) with UV transillumination to induce and capture a fluorescence signal ...
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bioRxiv - Biophysics 2023Quote: Proteins were resolved on 12% SDS-polyacrylamide gels and transferred to nitrocellulose membranes (GE Healthcare). The primary antibodies used were mouse anti-EHD2 (L-05 ...
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bioRxiv - Biophysics 2024Quote: ... Protein was further purified by size-exclusion chromatography using a Superdex 200 column (GE healthcare) in SEC buffer (25 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... Protein expression levels were visualized with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... Protein was further purified on a HiLoad 16/600 Superdex 75 pg column (GE Healthcare) in 10 mM Tris (pH 7.6 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated on 7.5-12% SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare). Blots were blocked 1 h with 10% dry milk ...
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bioRxiv - Microbiology 2024Quote: ... proteins were transferred to a 0.45-μm-pore-size nitrocellulose membrane (Hybond-ECL; GE Healthcare) and incubated with a α-HIS antibody (Genscript A00186) ...
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bioRxiv - Immunology 2023Quote: ... The protein solution was loaded on to a 1 ml Mono S column (GE Healthcare), eluted with a 20-bed volume linear gradient from 0 to 500 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein signals were detected by ECL Western Blotting Detection Reagent (GE Healthcare Life Sciences, RPN2109) with FUJI LAS-3000 system (Fujifilm ...
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bioRxiv - Biochemistry 2023Quote: ... pre-cleared protein was loaded via injection valve onto a Sephacryl S100HR column (GE Healthcare) (26/60 ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were run on SDS-PAGE gels and transferred onto nitrocellulose membrane (GE healthcare) via wet transfer for 1.5 hr at 100 V ...
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bioRxiv - Physiology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 mm NC) at 100V for 90 min on ice in transfer buffer (48mM Tris–HCl and 39mM glycine supplemented with 20% methanol) ...
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bioRxiv - Microbiology 2023Quote: ... The soluble protein was loaded onto a 1.7 mL cation-exchanger column (MonoS; GE Healthcare) pre-equilibrated with buffer A50 (50 mM Tris pH 7.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples (20µl) were injected onto a Superdex 200 10/300 Increase column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein sample was passed over a HiLoad 26/600 Superdex 200 column (GE Healthcare) pre-equilibrated with the equilibration buffer (100 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The running buffer for the immobilization of the proteins was PBSP (GE Healthcare Life Sciences). The binding assay was carried out using the same buffer and a flow rate of 30 μL/min at 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were purified by nickel affinity chromatography using Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2023Quote: ... The protein mixture was then loaded to Superdex 200 HiLoad 16/600 column (GE Healthcare) equilibrated with SEC buffer (50 mM Hepes pH 7.6 ...
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bioRxiv - Biophysics 2024Quote: ... The protein was further purified using a 5 mL HiTrap Heparin HP column (GE Healthcare), and then eluted with a linear gradient of 0.1-0.5 M NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein expression was visualized with an Amersham ECL Western Blot System (GE Healthcare Life Sciences). β-Actin served as a loading control ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were subjected to anion exchange chromatography using the HiTrap™ Q column (GE Healthcare) using the ÄKTA™ start system (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to a 0.45 µm Amersham Protran® nitrocellulose membrane (GE Healthcare, Amersham) before being incubated in primary antibody resuspended in 5 % milk-powder at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins separated by 12% Tris-glycine SDS–PAGE were transferred to nitrocellulose membranes (GE Healthcare) and processed for immunoblotting using an ECL Prime kit (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... whereas IgG3 antibodies were isolated with a HiTap Protein G High Performance column (GE Healthcare). Antibodies were dialyzed in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... coli M55244 cells (ATCC) and purified using Protein L resin (GE Healthcare Cat no. 17547802) with gravity flow affinity chromatography ...
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bioRxiv - Biophysics 2022Quote: ... All proteins were further purified on a 5 ml HiTrap Heparin HP column (GE Healthcare), equilibrated in their respective dialysis buffer and eluted by an NaCl gradient containing 1 – 1.5 M NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The target proteins were purified with HisTrap HP columns (GE Life Sciences, Velizy-Villacoublay, France). Further purification was achieved by size-exclusion chromatography in buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing desired protein was applied to either Glutathione-Sepharose resin (GE Healthcare Life Science) for GST purification or Ni-NTA Agarose resin (QIAGEN ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved protein was further purified using a HiTrap HP Heparin column (GE Healthcare, Illinois, USA), eluting with a linear gradient to 1.0 M KCl ...
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bioRxiv - Biophysics 2022Quote: ... The protein-containing supernatant was then applied to PD-10 Sephadex desalting column (GE Healthcare) to remove access of peptide/dye ...
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bioRxiv - Plant Biology 2022Quote: ... protein bands and radioactive bands were detected with an ImageQuant LA4000 imaging system (GE Healthcare) and a Typhoon Trio phosphorimager (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... The intensity of the resultant protein bands was quantified using ImageQuant TL software (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Protein was then purified from the supernatant using a StrepTrap HP column (GE Healthcare #28907547), followed by SEC purification over a S6 increase column (GE Healthcare #29091596 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were purified on a 5 ml HiTrap SP HP cation exchange column (GE Healthcare) and eluted with 1 M KCl ...
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bioRxiv - Neuroscience 2023Quote: ... Flag immunoprecipitates of IGF-1R-Flag were incubated with Protein G Sepharose beads (GE Healthcare). Beads were pelleted ...
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bioRxiv - Biochemistry 2023Quote: ... The antigen–antibody complexes were pulled down by 30 μl Protein A-Sepharose (GE Healthcare) and specific proteins were detected by the Western blotting analysis ...
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bioRxiv - Biochemistry 2023Quote: ... Binding of the proteins was evaluated using an anti-GST-HRP conjugate (GE Healthcare, RPN1236). For comparative evaluation of affinities ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were pre-cleared by incubating with protein A or G sepharose beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The spike proteins were further purified on a Superose 6 gel filtration column (GE Healthcare) in 25 mM Tris pH 7.5 + 150 mM NaCl using an AKTA Pure FPLC instrument (GE Healthcare ...