Labshake search
Citations for GE Life Sciences :
2351 - 2400 of 4700 citations for Rat Protein SET SET ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... The concentrated DnaA protein was subjected to size exclusion chromatography on an Äkta pure instrument (GE Healthcare). 200 μL of concentrated DnaA-6xHis was applied to a Superdex 200 Increase 10/30 column (GE Healthcare ...
-
bioRxiv - Systems Biology 2021Quote: ... The proteins were detected by Enhanced Chemiluminescence (ECL) using ECL Western Blotting reagents (Amersham, GE Healthcare, UK) or SuperSignal West Dura Extended Duration Substrate (Pierce ...
-
bioRxiv - Microbiology 2020Quote: ... The eluted protein was desalted against 1 x PBS buffer and digested with thrombin protease (GE Healthcare) in a ratio of 1 unit per μg at room temperature ...
-
bioRxiv - Systems Biology 2020Quote: ... denatured proteins were then separated via SDS-PAGE and transferred to a PVDF membrane (GE Healthcare, Amersham). Blots were preincubated in a blocking solution of 5% BSA in 0.2% TBST (0.1 M Tris base ...
-
bioRxiv - Plant Biology 2020Quote: ... the purified RCA protein was immobilized on a carboxy-methylated dextran sensor chip (CM5) chip (GE Healthcare) using an amine coupling method kit (GE Healthcare) ...
-
bioRxiv - Microbiology 2020Quote: ... Chromatin samples derived from 1.0×106 cells were precleared with protein G sepharose (GE Healthcare, Chicago, IL) and then 1-5 μg of each antibody was added to form immune complex ...
-
bioRxiv - Microbiology 2020Quote: ... supernatant containing VSG protein was loaded onto an anion-exchange column (Q-Sepharose Fast-Flow, GE Healthcare), which had been equilibrated with 10 mM phosphate buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant protein was purified from the filtered culture supernatant using HisTrap Excel columns (GE Healthcare Life Sciences). Each column was stored in 20% ethanol and washed with 5 column volumes (CV ...
-
bioRxiv - Microbiology 2020Quote: ... FLAG epitope-tagged protein levels were visualized using the Amersham ECL western blotting detection reagent (GE Healthcare). Thereafter ...
-
bioRxiv - Microbiology 2019Quote: ... Typically 100 µg of protein samples were injected into Superdex 200 10/300 increase column (GE Healthcare) by a Shimadzu autosampler coupled HPLC machine ...
-
bioRxiv - Cell Biology 2021Quote: ... between 600-900 μg of each CLASP1 protein was injected into a Superose 6 column (GE Healthcare) in a buffer containing 50 mM phosphate pH 7.6 ...
-
bioRxiv - Cell Biology 2021Quote: ... The next morning the cleaved protein was loaded on a 5 ml HiTrap HeparinHP column (GE Healthcare). The protein was eluted over a gradient from 100 mM to 2M KCl ...
-
bioRxiv - Biophysics 2020Quote: ... the protein solution was fractionated using a DEAE Sepharose Fast Flow anion exchange chromatography resin (GE healthcare) eluting with a gradient from 0 to 1 M NaCl and further fractionated by size-exclusion chromatography using a HiPrep™ 26/60 Sephacryl® S-500 HR column ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were then electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% (v/v ...
-
bioRxiv - Cell Biology 2021Quote: ... The immuno-complexes were collected with 50 μl of protein A sepharose (17-0780-01, GE Healthcare) at 4 °C for two hours with rotation ...
-
bioRxiv - Cancer Biology 2022Quote: ... Immunoreactive protein bands were visualized via enhanced chemiluminescence and scanned with a LAS-4000 system (GE Healthcare).
-
bioRxiv - Evolutionary Biology 2022Quote: ... The protein sample was further purified using a Superdex200 26/600 size-exclusion chromatography column (GE Healthcare), and fractions were collected and concentrated to 1.8 mg/ml in 10 mM Tris pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated and further purified on a Superdex 75 16/600 GL column (GE Healthcare), in a size exclusion buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Cell Biology 2022Quote: Equal amounts of protein were resolved on 10% SDS-PAGE and blotted onto nitrocellulose membranes (GE Healthcare). Immunoblots were blocked with 5% fat-free milk in PBS containing 0.1% Tween-20 for 1h at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... ActRIIB-Fc was isolated using affinity chromatography with Mab Select Sure Protein A (GE healthcare - Waukesha, WI), followed by dialysis into 10mM Tris ...
-
bioRxiv - Biochemistry 2022Quote: ... The tagless protein was further purified by anion exchange chromatography (Mono Q 10/100 GL, GE Healthcare) in 25 mM potassium phosphate (pH 7.5) ...
-
bioRxiv - Biochemistry 2022Quote: Samples were precleared by adding 60 μl of 20% Protein A Sepharose™ CL-4B (GE Healthcare) in IP Buffer (15 mM Tris-HCl ...
-
bioRxiv - Microbiology 2022Quote: ... the buffer exchanged protein was concentrated and injected on a SD200 10/300 increase column (GE Healthcare) equilibrated with 25 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2022Quote: ... Anti-TNFα IgG1 was purified on Protein A HiTrapHP columns (GE Healthcare Life Sciences, Little Chalfront, UK) using Akta-prime plus (GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified using size exclusion chromatography (Superdex 200 Increase 10/300 GL; GE Healthcare) to remove nonspecific proteins ...
-
bioRxiv - Biochemistry 2022Quote: ... Collected fractions containing membrane proteins were loaded onto a Superose 6 increase 10/300 column (GE Healthcare) and eluted with 30 mL of NCMN Buffer E ...
-
bioRxiv - Biophysics 2022Quote: ... the modified proteins were purified and separated from unbound dye through a gel filtration column (GE Healthcare illustra NAP Columns from Fisher ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were further purified by size exclusion chromatography using Superdex 200 16/60 column (GE Healthcare) equilibrated in buffer containing 25mM Tris-HCl pH8.0 ...
-
bioRxiv - Cell Biology 2022Quote: Samples were separated on SDS-polyacrylamide gel and proteins were transferred onto a nitrocellulose membrane (GE healthcare) using a wet transfer system (BioRad) ...
-
bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
-
bioRxiv - Immunology 2020Quote: ... the supernatants with the secretion of antibodies were collected and captured by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and dialyzed into phosphate-buffered saline (PBS) ...
-
bioRxiv - Biochemistry 2021Quote: ... and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare), followed by a TEV protease cleavage at 16°C overnight ...
-
bioRxiv - Molecular Biology 2019Quote: ... The proteins were further purified by size exclusion chromatography on a Superdex200 16/60 column (GE Healthcare).
-
bioRxiv - Neuroscience 2020Quote: ... The protein was purified using gel filtration (Superdex75 or 200 Hi-load column, GE Healthcare, Chicago, IL). The peak fractions were pooled and concentrated using filters of appropriate cut-offs (EMD Millipore ...
-
bioRxiv - Immunology 2021Quote: ... Rhesus sera or plasma antibodies were first purified by affinity chromatography using Protein A Sepharose (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The GST part was cleaved off from the recombinant protein using PreScission Protease (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... the protein was concentrated and further purified by gel filtration using a superpose 6 increase (GE healthcare) in TBS containing 0.01% LMNG ...
-
bioRxiv - Bioengineering 2021Quote: ... The protein was further purified by size-exclusion chromatography using a HiLoad Superdex 200PG column (GE Healthcare) with PBS as an eluent ...
-
bioRxiv - Neuroscience 2020Quote: ... Proteins were purified by immobilized metal-affinity chromatography (IMAC) using pre-packed nickel Sepharose columns (GE Healthcare), or in batch mode on TALON® beads (Takara Bio ...
-
bioRxiv - Immunology 2020Quote: ... and the protein was separated on a HiPrep 16/60 Sephacryl S-200 HR column (GE Healthcare) equilibrated with TBS ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved by 10% SDS PAGE and transferred on a nitrocellulose membrane (Protran BA83, GE Healthcare) using a semi-dry system (Bio-Rad Laboratories S.r.l.) ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were either stained with Coomassie brilliant blue G-250 or transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in 0.05% Tween TBS at room temperature for 1 h and then incubated at 4 °C overnight with a specific polyclonal serum produced by immunization of mice with RBD produced in HEK-293T cells ...
-
bioRxiv - Biochemistry 2020Quote: ... the proteins were buffer exchanged into the appropriate buffer using an Illustra NAP-5 column (GE Healthcare).
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were then loaded on a Superdex 200 10/300 GL Tricorn gel filtration column (GE Healthcare), and eluted with 1.5 column volumes of gel filtration buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Biophysics 2020Quote: ... the eluted protein was loaded onto a size exclusion column (Superdex 200 16/600 column, GE Healthcare) and eluted with 20 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 250 µl of antiserum were coupled to 60 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed three times in CPB and supplemented with 6 µl fresh rPAS ...
-
bioRxiv - Molecular Biology 2022Quote: ... Concentrated protein was loaded onto a 16/600 Superdex 200 gel filtration column (GE Healthcare Piscataway, NJ). The buffer conditions were the same as in Ni2+-NTA affinity chromatography but with 20 mM NaCl and no imidazole ...
-
bioRxiv - Neuroscience 2022Quote: ... The Twin-Strep-tagged protein was purified via affinity chromatography using a StrepTrap HP column (GE Healthcare). Next ...
-
bioRxiv - Microbiology 2022Quote: ... Periplasmic proteins were extracted and CusF was purified on a 5 ml StrepTrap HP column (GE healthcare) equilibrated with buffer A (10 mM NaPi ...