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Citations for GE Life Sciences :
2351 - 2400 of 5453 citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10–50 μg) from the total extract were separated on 8–10% SDS–polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Chicago, IL) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... The elimination of imidazole was achieved by gel filtration on columns filled with 10 ml of Sephadex G-25 (GE Healthcare Life Sciences) previously equilibrated with ice-cold PBS ...
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bioRxiv - Genetics 2019Quote: ... The reaction was incubated at 15 °C for 40 min and then purified using an illustra Sephadex G-50 column (GE Healthcare, Buckinghamshire, UK).
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bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant after centrifugation at 125,000 g for 45 min was applied to a Ni-NTA column (GE Healthcare, Chalfont St Giles, UK) equilibrated in buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were centrifuged for 20 min at 23000 × g at 4°C and filtered using Whatmann Mini-Uniprep filtering vials (GE Healthcare, Buckinghamshire, UK) with a regenerated cellulose membrane (0.2-μm pore size).
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bioRxiv - Genomics 2021Quote: ... were 5’ end-labelled with [y-P32]-ATP by T4 polynucleotide kinase (New England Biosciences) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2022Quote: ... bacterial extracts were centrifugated for 20 min at 18,000 x g at 4°C and the supernatant was incubated with 1.5 ml of a GSH-sepharose 4B resin (GE Healthcare 17-0756-01) for 1.5 h at 4°C on a rotating wheel ...
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bioRxiv - Neuroscience 2022Quote: ... The probe was end labeled with 35S-dATPα using terminal deoxytransferase and extracted using Illustra Microspin G-25 columns (GE Healthcare, North America). Each slide was then hybridized with 600,000 cpms of labeled probe in 125 μl of hybridization buffer and slides incubated overnight in a humid chamber warmed to 37° C ...
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bioRxiv - Microbiology 2023Quote: ... Unencapsulated ANTS and DPX were removed by gel filtration through a 5 mL HiTrap Desalting Sephadex G-25 resin column (GE Healthcare Life Sciences). To ensure the correct size and obtain LUVs ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μg DNA loaded per lane was separated by electrophoresis on 1% agarose gels and transferred to Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Neuroscience 2023Quote: ... followed by filtration (through 100 μm mesh) and enrichment for leukocytes by gradient centrifugation (40% Percoll GE healthcare, 600 x g, 15 min). To prepare single cell suspensions from spleen tissues ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then incubated for 30 minutes at room temperature and excess copper was removed by gel filtration using a PD MidiTrap G-25 desalting column (GE Healthcare; Uppsala, Sweden) equilibrated with acetate buffer (25 mM sodium-acetate ...
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bioRxiv - Cell Biology 2023Quote: ... The mixture was separated by ultracentrifugation at 100 000 x g for 30 min at 4 °C and supernatant was loaded onto a HisTrap HP column (GE Healthcare, Freiburg, Germany) preequilibrated with 50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... Cell suspensions were filtered through a 70 µm cell strainer, centrifuged (5 min, 500 g) and pellets were resuspended in DMEM containing 30% stock isotonic Percoll (SIP, GE Healthcare, Diegem, Belgium). Hereafter ...
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bioRxiv - Molecular Biology 2024Quote: ... Digested samples were passed through pre-washed (4x with 700 μl polysome buffer for 1 min at 450 x g) Sephacryl S-400 HR spin columns (GE healthcare, currently Cytiva), and centrifuged for 2 min at 450 x g at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... Secreted proteins were purified from the supernatant by sequential Ni2+ (HisTrap excel; GE Healthcare; equilibrated in 25 mM KPi pH 7.8 ...
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bioRxiv - Biophysics 2020Quote: ... The filtrate was analysed by fast protein liquid chromatography (AKTApurifier, GE Healthcare Life Sciences) using a MonoQ column (5/50 GL ...
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bioRxiv - Biophysics 2020Quote: ... the protein was separated on a RESOURCE™ reversed phase chromatography column (GE Healthcare) using a 0-70 % acetonitrile gradient ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were loaded onto an Äkta Pure chromatography setup (GE Healthcare, Uppsala, Sweden) equipped with a Superdex 200 26/60 column or Superdex 200 16/60 column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified using ion-exchange Hi-Trap Q HP-(GE Healthcare). The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... was bound to 20 μL (bed volume) FastFlow Protein A Sepharose beads (GE Healthcare) by incubating for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and the GST-fusion protein was purified using Glutathione-Sepharose affinity columns (GE Healthcare). The proteins were finally eluted in PBS containing 20 mM glutathione (pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was concentrated by centrifugal ultrafiltration and applied to a Superdex-200 (GE Healthcare) size exclusion chromatography column ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected by enhanced chemiluminescence (ECL Prime Western blotting detection reagent; GE Healthcare).
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bioRxiv - Genetics 2021Quote: ... Protein bands were visualised using Enhanced Chemiluminescence (ECL) Western blotting detection reagents (GE Healthcare) and exposure of the membrane to Kodak BioMax XAR film (Sigma) ...
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bioRxiv - Genetics 2021Quote: ... and proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 NC, GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected by enhanced chemiluminescence (ECL Prime Western blotting detection reagent, GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated on a HiLoad Superdex 200 Increase 10/300 column (GE Healthcare) and plotted against BioRad Standards to determine size ...
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bioRxiv - Plant Biology 2021Quote: The purified proteins were loaded onto a Superdex 200 GL 10/300 (GE Healthcare) column and eluted with a buffer containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Developmental Biology 2020Quote: ... and proteins were detected using Amersham Western Blotting Detection Reagent (GE Healthcare, Chicago, IL). Western blot membranes were probed with mouse anti-RFP (RF5R Invitrogen ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... Protein was detected using an Amersham ECL Western Blotting Analysis System (GE Healthcare RPN2109), and developed onto film or imaged using a Biorad Chemidoc gel imager ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein purification was performed using Nickel NTA columns (17-5248-01, GE Healthcare, USA) or beads (7066-4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fc and non-digested IgG were removed via Protein A affinity chromatography (GE Healthcare). 2E10.E9 Fab was subsequently purified via size-exclusion chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Molecular Biology 2020Quote: ... Then the target proteins were loaded onto a HiTrap Q column (GE Healthcare, USA). An increasing linear gradient of NaCl concentration was applied onto the HiTrap Q column for further purification ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein levels were quantified with the ImageQuant TL software (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2020Quote: ... protein was immobilized to the CM5 sensor chip (GE Healthcare, Cat#. BR-1005-30) at 6.0 μg/mL concentration using 10mM sodium acetate (pH 5.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The dialyzed protein was then passed through a Hi-Trap Chelating column (GE Healthcare) and the unbound material was collected ...
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bioRxiv - Molecular Biology 2021Quote: ... cleaved protein was loaded onto a Superdex 75 10/300 GL column (GE Healthcare) in Ni-buffer A ...
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bioRxiv - Bioengineering 2022Quote: ... and IgG was purified with Protein A sepharose beads (GE Healthcare 17-5280-04).
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bioRxiv - Cell Biology 2022Quote: ... 3 mg total protein was loaded on a Superose 6 HR10/30 (GE Healthcare) column equilibrated with PBS ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was bound to an anion exchange column (MonoQ 16/10, GE Healthcare) and eluted with a linear salt gradient up to 1 M NaCl ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged mammalian ATG8 fusion proteins were cloned into pGEX-4T-1 (GE Healthcare) and expressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2019Quote: Western blotting was carried out following protein transfer onto a nitrocellulose membrane (GE Healthcare) on a TE 77 PWR Semi-Dry Transfer Unit (45 mA/gel and 30 min with a NOVA Blot kit ...
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bioRxiv - Biochemistry 2019Quote: ... the protein was loaded onto a pre-equilibrated QHP ion exchange column (GE Healthcare) and eluted with buffer B containing increasing concentrations of NaCl (0.1-1 M) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were purified by immobilised metal affinity chromatography using nickel-Sepharose resin (GE Healthcare) equilibrated in buffer A ...
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bioRxiv - Plant Biology 2019Quote: ... purified proteins were run down a Superdex™ 75 10/300 column (GE Healthcare) at 0.5 ml/min either alone or mixed to assess complex formation (mixtures were incubated on ice for 2 hours prior to experiment) ...
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bioRxiv - Microbiology 2019Quote: ... MBP-fusion proteins were purified with amylose resin following the manufacturer’s instructions (GE Healthcare, Pierce ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was passed over a size exclusion column (Superdex 200, GE Life Sciences) in buffer GF (buffer A plus 300 mM NaCl and 1 mM dithiothreitol (DTT)) ...
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bioRxiv - Biochemistry 2020Quote: ... The target protein was further purified using MonoQ and Superdex G75 columns (GE Healthcare).