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Citations for GE Life Sciences :
2301 - 2350 of 3120 citations for 7 bromo 1 2 3 4 tetrahydroisoquinoline 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... ATP and MgCl2 were then added to a final concentration of 1 and 20 mM and then applied to a HiTrap Q column (GE Life Sciences). Separation occurred over an 18 CV gradient from 100% IEX1 to 100% IEX2 buffers [20mM Tris pH 8.0 (RT ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by addition of 6 mM β-mercaptoethanol and mixtures were applied to a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by buffer exchange into G buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA) using desalting column (GE Healthcare; cat#17085101). To remove the GST tag ...
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bioRxiv - Biochemistry 2022Quote: ... The complex formed in the 1:2.5 mixture was purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in assay buffer and run at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the patients underwent a T1 weighted 1 mm isometric anatomical MRI scan using a 3T Signa HDx scanner (GE Healthcare, Chicago, Illinois). Following the implant ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl of each mucus sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), left to dry out ...
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bioRxiv - Neuroscience 2022Quote: ... Images were captured one frame every 1 min 30 sec over 5 h using a DeltaVision Imaging System (Applied Precision, Bratislava, Slovakia).
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bioRxiv - Molecular Biology 2022Quote: ... 10 or 15 % 29:1 acryl:bis-acrylamide) and transferred on Amersham™ Protran™ 0.45 μm nitrocellulose membranes (GE Healthcare, Cat# 10600033) at 4°C for 1 hr at 110V ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed in PBT and incubated with HRP conjugated-anti-rabbit IgG (GE Healthcare # NA9340, 1:5000 in blocking solution) at room temperature for 1 hour and washed twice in PBT ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then diluted to a final salt concentration of low salt buffer conditions (100 mM KCl, 50 mM HEPES, 1 mM MgCl2) and bound to HiTrap SP FF (GE Healthcare, USA) anion exchange column ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM imidazole at pH 8) and loaded on a nickel affinity chromatography column (1 mL HisTrap, GE Healthcare, 17-5247-01). Bound proteins were eluted in elution buffer (20 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2024Quote: ... The tagged proteins were detected using HRP conjugated sheep anti-mouse or anti-rabbit IgG antibody (1:10000, GE Healthcare, Uppsala, Sweden) and visualized using the Amersham ECL Western blot detection reagent (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... plasma or CSF probes were incubated in 25 μl for 1 hour at room temperature (diluted with PBS containing 5% Amersham ECL Blocking Agent, GE Healthcare RNP2125), followed by additional washing steps and incubation with an anti-mouse secondary antibody conjugated with the MSD Sulfo-tag for detection ...
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bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... CaM was eluted in buffer containing 1 mM EDTA then further purified using anion exchange with a Resource Q column (GE Life Sciences) before dialysis into water and lyophilization in a vacuum concentrator.
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed 3×10mins with TBS-T and 1×10mins with TBS before developing the signal by incubating the membrane in Amersham Hyperfilm ECL for 5 minutes (GE Healthcare, UK).
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was washed 5 times in PBS-T before it was incubated with the secondary antibody goat α-mouse IgG (H&L)-HRP (1:10’000, GE healthcare #NXA931). After 1 h incubation ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were injected two times/embryo with mRNAs at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNAs or plasmid DNA at two-cell or four-cell stage using a PicoSpritzer setup in 1/3x Modified Frog Ringer’s solution (MR) with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing Gentamycin ...
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bioRxiv - Immunology 2022Quote: Serum samples from HIV-1-infected individuals were heat-inactivated at 56 °C for 40 min and incubated with Protein G Sepharose (GE Life Sciences) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-3xFLAG-V1H(1-351) was further purified by gel filtration on a Superdex 200 10/300 GL column (GE Life Sciences) into the appropriate buffer for the subsequent application ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of fosmid or BAC DNA was labelled by nick-translation with fluorescent dUTPs (Abott Molecular or GE HealthCare Life Science) for 3 h at 15°C as described previously41 ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was diluted 1:10 v/v with 20 mM Tris 8.0 and further purified with anion exchange chromatography (HiTrapQ column, GE Healthcare Life Sciences) using a NaCl gradient of 50 mM to 500 mM in 20 mM Tris 8.0 ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was washed with 0.1% TBST and incubated with corresponding secondary antibody conjugated to Horse Radish Peroxidase (HRP) at 1:10000 (GE life sciences; NA934) dilutions for one hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and detected with a 1:10000 goat anti rabbit secondary antibody conjugated with HRP and imaged with ECL (GE healthcare NA931, NA934).
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bioRxiv - Genetics 2024Quote: ... Cell debris was removed by centrifugation and the supernatant was incubated with 1 mL of equilibrated Ni-Sepharose 6 Fast Flow resin (GE Life Sciences) and gently mixed in batch for 1 hr at 4° C ...
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bioRxiv - Biophysics 2021Quote: ... 4°C for 30 minutes and then loaded onto a 5mL NiNTA affinity column (HisTrap HP, GE Healthcare) pre-equilibrated with the lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µl of a Protein A sepharose (nProtein A Sepharose 4 fast flow, GE Healthcare, 17-5280-01) suspension was added and incubated for 1 hour at 4°C on a rotator ...
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bioRxiv - Cell Biology 2020Quote: Sheared chromatin was pre-cleared with 50 µl protein A Sepharose 4 Fast Flow beads (GE Healthcare, Germany) per 500 µg chromatin for 2h ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: Recombinant ATG8 proteins were then coupled to cyanogen bromide-activated sepharose 4 Fast Flow (CNBr-sepharose, GE Healthcare) as described (Kavran & Leahy ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C) the sample was applied onto a HiLoad Superdex 200 (16/60) column (GE Healthcare, now Cytiva). Separation was performed at a flow rate of 0.8 mL/min in SEC buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
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bioRxiv - Cancer Biology 2020Quote: ... The triarylmethyl EPR oxygen tracer OX063 (methyl-tris[8-carboxy-2,2,6,6-tetrakis[2-hydroxyethyl]benzo[1,2-d:4,5-d0]-bis[1,3]dithiol-4-yl]-trisodium salt) was obtained from GE Healthcare.
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bioRxiv - Cell Biology 2021Quote: ... the lysates were incubated 16 hours at 4°C with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01). Next ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and mononuclear cells isolated by density gradient centrifugation on a discontinuous 35%/70% Percoll (GE Healthcare) gradient (2000 rpm [931 g] ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Images (4 fields/well at 20x magnification) were captured through an INCell Analyzer 6000 HCA system (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
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bioRxiv - Biochemistry 2019Quote: Removal of the His6-tag (Rab8a) was performed by overnight incubation at 4 °C with thrombin (GE Healthcare), followed by a second Ni2+-agarose column ...
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bioRxiv - Immunology 2020Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied and circulated on a Glutathione Sepharose 4 Fast Flow 16/10 column (GE Healthcare) for 16 h ...
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bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... followed by size-exclusion chromatography at 4 °C on a HiLoad Superdex 200 pg (GE Healthcare Life Sciences) column pre-equilibrated in the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Plant Biology 2022Quote: ... Immunoprecipitation was performed by incubating sheared chromatin with Protein G Sepharose™ 4 Fast Flow beads (GE Healthcare) and antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... incubated overnight at 4°C and subjected to size exclusion chromatography using Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...