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Citations for GE Life Sciences :
2201 - 2250 of 3218 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... or using an ÄKTA Prime purification system (Amersham Biosciences) equipped with 5 mL HisTrap column (GE Healthcare), with an imidazole gradient of buffer B (20 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then loaded onto a 5 ml HiTrap SP FF column (GE Healthcare Life Sciences) followed by gradient elution with elution buffer SB (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysates were incubated with glutathione sepharose (1 ml bed volume; GE Healthcare #17-0756-01), washed and eluted by the addition of 2 x 1.5 ml of 10 mM reduced L-glutathione (Sigma #T6066 ...
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bioRxiv - Immunology 2022Quote: ... the sample was again loaded onto a HisTrap™ HP 5 ml column (GE Healthcare, Diegem, Belgium). The flow-through was collected and concentrated before injection onto a HiLoad® 16/600 Superdex® 75 pg column (GE Healthcare Diegem ...
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bioRxiv - Physiology 2022Quote: ... Leukocytes were separated from debris by resuspending the pellet in 5 ml 33% Percoll solution (GE Healthcare). Erythrocytes were lyzed using an ACK lysis buffer (155 mM NH4Cl ...
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bioRxiv - Microbiology 2022Quote: ... The dialysed sample was loaded onto Ni Sepharose® (24 ml) (GE Healthcare Europe GmbH, Freiburg, DE) previously equilibrated with buffer E ...
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bioRxiv - Cell Biology 2022Quote: ... then loaded onto a 5 mL Histrap column (Cytiva) connected to an FPLC AKTAxpress system (GE Healthcare). After affinity binding ...
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bioRxiv - Immunology 2022Quote: ... GPC-I53-50A was concentrated to at least 2 µg/ml using Vivaspin centrifugal filters (GE Healthcare). To generate complexes ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with 1 mL IgG beads (IgG Sepharose 6 Fast Flow, GE Healthcare, 17096902) for POT1 or 1 mL amylose beads (New England BioLabs ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.5 mM EDTA) and loaded onto a 5 mL RESOURCE Q anion exchange chromatography (GE Healthcare). Hsp104 was eluted via linear gradient of buffer Q+ (20 mM Tris pH = 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mL of supernatant was collected and loaded to a Sephacryl S-300 HR column (GE healthcare) pre-balanced with G-buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The eluent was bound to glutathione sepharose beads (10 mL, GE Healthcare Life Sciences, USA, 6 h), washed with 1 L wash buffer ...
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bioRxiv - Immunology 2022Quote: ... Filtrated culture supernatants were purified with a 5 ml HisTrap FF Crude column (GE Healthcare Life Sciences) previously equilibrated in Buffer A (20 mM NaH2PO4 ...
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bioRxiv - Neuroscience 2021Quote: ... Sample was concentrated to ∼5 mg/mL and injected onto a Superose 6 Increase column (GE Healthcare) equilibrated in SEC buffer ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Microbiology 2022Quote: ... Periplasmic proteins were extracted and CusF was purified on a 5 ml StrepTrap HP column (GE healthcare) equilibrated with buffer A (10 mM NaPi ...
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bioRxiv - Molecular Biology 2022Quote: ... Survivin was purified by immobilized metal ion chromatography (IMAC) with a 5 ml HisTrap FF (GE Healthcare) column followed by overnight dialysis in 50 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The cleaved receptor solution was then loaded onto a 5 mL HiTrap SP HP column (GE Healthcare) using an Akta Start system (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... Siz1 (167-508) or Siz2 (130-490) was purified from MBPTrap HP 1 mL column (GE Healthcare) with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... the dialysed WT RAD51 containing sample was loaded onto a 5 ml HiTrap Heparin column (GE Healthcare) and eluted via a linear gradient of 200 mM – 600 mM KCl ...
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bioRxiv - Biochemistry 2019Quote: ... Peak fractions were applied on a 24 ml Superdex200 10/300 GL size-exclusion column (GE Healthcare), and fractions containing monometic F86E RAD51 were pooled and dialysed in TEG100 ...
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bioRxiv - Biochemistry 2019Quote: ... Lystate was clarified by centrifugation and loaded onto HisTrap™ High-Performance 5 mL columns (GE Healthcare) using a peristaltic pump ...
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bioRxiv - Plant Biology 2019Quote: ... one of the 1 mL supernatant was desalted by PD-10 desalting columns (GE Healthcare, Pittsburgh, PA) according to manufacturer’s instructions and eluted with 3 mL IP buffer ...
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bioRxiv - Biophysics 2020Quote: ... The plexin protein in the supernatant was captured using a 1 mL HisTrap FF column (GE Healthcare) and eluted by Buffer B containing 10 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysates were cleared by centrifugation and loaded onto a 5 ml HisTrap HP column (GE Healthcare). After washing with buffer A (50 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was loaded onto a 1 ml HiTrap Heparin HP column (GE Healthcare, 17-0406-01) and eluted with a linear gradient from buffer A (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... and the supernatant loaded onto a freshly charged 5 mL HisTrap FF Ni-affinity column (GE Healthcare) at 1 mL/min at 4 °C (50 mM Tris buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Eluted protein was further purified using an anion exchange column (HiTrap Q HP 5 ml; GE Healthcare) before buffer exchange into PBS and storage at –20°C.
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bioRxiv - Biochemistry 2020Quote: ... and the cleaved sample was loaded onto a HisWash2 equilibrated 5 ml HisTrap HP column (GE Healthcare). The flow-through was collected as 2 ml fractions ...
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bioRxiv - Immunology 2020Quote: ... Microglia were separated from debris by resuspending the pellet in 5 ml 33% percoll solution (GE Healthcare). Mast cells and macrophages were obtained from the peritoneal cavity by lavage with 5 ml cold PBS + EDTA (2 mM ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to a 10 ml HisTrap FF (GE Healthcare) equilibrated in washing buffer (lysis buffer without protease inhibitors) ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to a 5 ml GSH column (GE Healthcare) equilibrated in washing buffer (lysis buffer without DNAse and PMSF) ...
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bioRxiv - Biochemistry 2019Quote: ... passed through a 0.2 µm filter and applied to a 5 ml HisTrap column (GE healthcare, USA). The bound protein was eluted with a gradient of buffer B (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Ion exchange chromatography was performed using either 6 ml RESOURCE S or RESOURCE Q column (GE Healthcare) pre-equilibrated with 5% of buffer B (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... and loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare, Cat# 17-5174-01) size exclusion column equilibrated in 20 mM Tris [pH 8.0] ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was applied to a 5 mL HisTrap HP Ni2+-NTA IMAC column (GE Healthcare) pre-equilibrated with lysis buffer and washed with 50 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... to < 1 mL and injected onto a SuperdexTM 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in Gel Filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biophysics 2020Quote: ... and the clarified cell extract was loaded onto a 5-mL HisTrap HP column (GE Life Sciences) equilibrated in HisTrap Buffer A ...
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bioRxiv - Genetics 2019Quote: ... This diluted sample was then loaded onto a 5 mL Hi-Trap Q HP column (GE Healthcare) and eluted over a linear gradient of 150 mM to 1000 mM NaCl with the protein eluting at 225 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... 100μg/ml of streptomycin and 5% fetal calf serum (FCS; HyClone, GE Healthcare, South Logan, UT, U.S.A.) at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Microbiology 2020Quote: The heterologous proteins containing His-tags were purified via 1 ml Ni-NTA HisTrap columns (GE Healthcare). The cells were lysed in 50 ml lysis buffer containing 50 mM Na-phosphate buffer pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... and size-exclusion chromatography was performed using a 24-ml Superdex 200 10/300 column (GE Healthcare) with 20 mM Tris (pH 7.5 ...
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bioRxiv - Immunology 2020Quote: IgGs were purified from plasma samples using 5-mL HiTrap MabSelect SuRe columns (GE Healthcare Life Sciences). Heat-inactivated plasma was diluted 10-fold with cold PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... the HisTrap FF column was additionally coupled to a HiTrap Heparin HP 5-ml column (GE Healthcare). Both proteins were subjected to TEV protease cleavage at 4 °C overnight and another HisTrap FF 5-ml column purification step ...
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bioRxiv - Molecular Biology 2021Quote: ... Extract was prepared as above and was passed through a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 10 CV of IMAC buffer ...
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bioRxiv - Immunology 2021Quote: ... affinity chromatography with in-line dialysis in PBS via HiTrap 5 ml desalting columns (GE Healthcare – 29048684) using an Akta™ Pure system (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... 2.5 mL was loaded into a HiPrep Sephacryl S-400 HR 16-600 SEC column (GE Healthcare) equilibrated with TBS using an ÄKTA Pure 25 system (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... A final anion exchange purification step was performed using a Resource Q 6 ml column (GE Healthcare), using 50 mM Tris HCl pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were clarified by centrifugation and purified through a 5 mL HisTrap FF column (GE Healthcare), using theÄKTA Pure chromatography system (Cytiva) ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to affinity purification with a His-Trap HP 5 mL (GE Healthcare) column ...