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Citations for GE Life Sciences :
2201 - 2250 of 4960 citations for 7 Methyl 1 5 dioxo 1 2 3 5 tetrahydro indolizine 6 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was finally concentrated to 1–10 mg mL−1 using centrifugal concentrators with a cut-off at 100 kDa (Vivaspin 100, GE Healthcare), frozen in liquid nitrogen and stored at −80 °C in storage buffer containing 50 mM Mes-KOH (pH 7) ...
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bioRxiv - Biophysics 2024Quote: ... Pooled elution fractions were subjected to a 2nd FPLC stage using a 1 ml HiTrap Heparin-HP 1 ml column (GE Healthcare). The bound MBP-XerC was washed using heparin loading buffer [50 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were incubated with primary antibodies diluted 1/500 to 1/1000 followed by TBS-T washes and incubation with HRP-conjugated secondary antibodies (GE healthcare). The signal was visualized by enhanced chemiluminescence with Luminata Forte Western HRP Substrate (Millipore ...
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bioRxiv - Systems Biology 2024Quote: ... The Bone marrow donors was diluted to a ratio 1:1 with PBS and layered on Ficol density gradient (GE Healthcare), followed by a centrifugation step for 20 minutes at 850 g at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... heparin-treated blood was diluted 1:1 with sterile PBS and carefully layered onto Ficoll-Paque (GE Healthcare Bio-Sciences, Sweden). The tubes were centrifuged at 400 x g for 30 min at 20°C ...
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bioRxiv - Physiology 2019Quote: Cardiac dimensions and contractility were assessed by right para-sternal transthoracic echocardiography (Vivid 7; GE Healthcare, UK) in non-sedated conscious sheep gently restrained in a supine position ...
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bioRxiv - Microbiology 2023Quote: ... of which 450 µl were subjected to isoelectric focusing on pH 4-7 IPG strips (GE Healthcare), followed by SDS-PAGE with 12 % acrylamide gels ...
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bioRxiv - Biophysics 2020Quote: ... In the first step the ENTH-domain was isolated from the soluble cell lysate as GST fusion protein by using GSTrapFF™ 5 ml columns together with the ÄKTAPrime Plus system (GE Healthcare Life Science, Chalfont St Giles, UK). The cell lysate was injected onto the column (0.4 ml/min ...
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bioRxiv - Microbiology 2024Quote: ... 2 µMnt ssDNA (Ovio 45) and 2 mM ATP (GE Healthcare) in a 40 µL reaction solution containing 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2019Quote: ... The bioreactors were inoculated at a target cell density of 0.4 × 106 cells/mL in ActiPro batch media and fed daily beginning on Day 3 (3% (v/v) Boost 7a and 0.3% (v/v) Boost 7b (GE Healthcare)) ...
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies coupled to horseradish peroxidase (HRP) were applied for 1 h: sheep anti-mouse (#NA9310V, GE Healthcare, 1:4000), donkey anti-rabbit (#NA9340V ...
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bioRxiv - Systems Biology 2022Quote: ... was combined with 250 μg carboxylated paramagnetic beads (1:1 mix of hydrophiclic and hydrophobic Sera-Mag SpeedBead Carboxylate-Modified, GE Life Sciences) and 750 μl ethanol ...
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bioRxiv - Neuroscience 2022Quote: ... distilled water was replaced with a 1:1 dilute of non-ionic iodine Omnipaque 350 contrast agent (Iohexol, 350 mg iodine/ml; GE Healthcare AS) in normal saline via 3 ml syringe ...
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bioRxiv - Microbiology 2024Quote: ... the following HRP conjugated secondary antibodies were used: for Cas9 - anti-mouse (1:10,000 in 1% milk PBS-T, GE Healthcare, code NA931V); for γ-H2A - anti-rabbit (1:2000 in 1% milk/PBS-T ...
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bioRxiv - Physiology 2022Quote: ... The specific proteins were detected with horseradish peroxidase (HRP) conjugated secondary antibodies (1:5000 dilution; CtNo_NA934, GE Healthcare, 1:5000 dilution; CtNo_NA931, GE Healthcare, Tokyo, Japan), developed with Immobilon Western Chemiluminescent HRP Substrate (CtNo_WBKLS0100 ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies were horseradish peroxidase (HPR)-conjugated (Sigma-Aldrich A0545, anti-rabbit 1:10000; GE Healthcare NA931V, anti-mouse 1:5000). Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
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bioRxiv - Biochemistry 2023Quote: The DNA sequences encoding the DNA binding domains of Pdr1p (residues 1-207) and Pdr3p (residues 1-184) were amplified by PCR from the Yeast Genomic Tiling Collection (GE Healthcare YSC4613). The PCR products were inserted into the pMCSG7 vector by ligation independent cloning ...
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bioRxiv - Cancer Biology 2023Quote: WDR5 (residues 22-334 or 1-334) and PTENα (residues 1-576) proteins were further purified using a Superdex200 gel-filtration column (GE Healthcare, 28989335) with a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... to a ratio of 1 to 1 before being layered onto Ficoll Paque (1.077 g/ml; GE Healthcare Europe GmbH, Freiburg, Germany). After centrifugation (800 x g for 25 min) ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then washed in TBST and incubated for 1 hour at room temperature with HRP-conjugated secondary antibody (1:10000 in PBS; GE Healthcare/Biocat) Membranes were washed three times in TBST before bands were visualised using ECL (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... followed by size exclusion chromatography using a Superose 6 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... and size-exclusion chromatography (Superose 6 Increase 10/300 GL, GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... and 150 mM NaCl (Superose 6 Increase 10/300 GL, GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... and purified on the Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer GF (10 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2021Quote: ... Spikes were further purified by Superose 6 increase 10/300 (GE healthcare) size-exclusion column in a buffer containing 2mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... for loading onto a Superose 6 Increase 10/300 GL (GE Healthcare) column equilibrated in storage buffer (50 mM HEPES pH 7.3 ...
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bioRxiv - Biochemistry 2021Quote: ... concentrated and loaded onto a Superose 6 Increase 10/300 (GE Healthcare) size-exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2020Quote: ... and performed size exclusion chromatography (SEC, Superose 6, 10/300, GE Healthcare) in Wash buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Purified protein was then loaded onto a Superose-6 column (GE Healthcare) in 20 mM HEPES pH = 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... and loaded onto a Superose 6 increase column (GE Healthcare, Chicago, IL) on an NGC system (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... and subjected to size exclusion on a Superose 6 column (GE Healthcare) in 10 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... connected to SEC Superose 6 Increase column (10/300 GL, GE Healthcare) at room temperature with a flow rate of 0.5 ml/min in sodium phosphate buffer A (20 mM Na2HPO4 * 2 H2O ...
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bioRxiv - Biochemistry 2021Quote: ... followed by size exclusion chromatography using Superose 6 Increase column (GE Healthcare) equilibrated in 200 mM NaCl ...
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bioRxiv - Microbiology 2022Quote: ... reloaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare) and analyzed by SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... and subsequently run on a Superose 6 size exclusion column (GE Healthcare). Samples were pre-screened for sample quality by negative stain transmission electron microscopy and then immediately prepared on cryoEM grids.
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bioRxiv - Microbiology 2020Quote: ... and further enriched using a Superose 6 gel-filtration column (GE healthcare), in a buffer containing 25 mM NaHEPES (pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... before being run over a Superose 6 size-exclusion column (GE Healthcare) in 2 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... by loading onto a Superose 6 Increase 10/300 GL (GE Healthcare) previously equilibrated in gel filtration buffer (30 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... and further purification by RESOURCE Q 6 mL column chromatography (GE Healthcare). Finally ...
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bioRxiv - Biophysics 2020Quote: ... Deglycosylated protein was then purified using a Superose 6 column (GE healthcare) equilibrated with buffer A ...
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bioRxiv - Biophysics 2019Quote: ... were applied to a Superose 6 10/300 GL column (GE Healthcare) previously equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2019Quote: ... containing a bed of 550 mL sepharose 6 Fast Flow (GE Healthcare). Eluted plasmid DNA from was precipitated with isopropanol ...
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bioRxiv - Biophysics 2020Quote: ... and 150 mM NaCl (Superose 6 Increase 10/300 GL, GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... and resuspended in 6 ml of 38% isotonic Percoll® (GE Healthcare) before a 25 min centrifugation at 800 G without neither acceleration nor brake ...
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bioRxiv - Molecular Biology 2020Quote: ... filter and run on a Superose™ 6 Increase column (GE Healthcare).
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bioRxiv - Molecular Biology 2022Quote: ... and purified on a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer GF (10 mM HEPES–NaOH pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... or a Superose 6 prep grade XK 16/600 column (GE Healthcare). Columns were equilibrated in Ndc80 buffer (50 mM Hepes ...