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Citations for GE Life Sciences :
2151 - 2200 of 4142 citations for Recombinant Human UBE2L6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... the reducing agents in the protein solution were removed with a PD10 desalting column (GE Healthcare), using a buffer based on 50 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... the protein was further purified using a size-exclusion column (Superdex, GE Healthcare, Chicago, Illinois, USA).
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bioRxiv - Biophysics 2020Quote: ... The proteins were purified by successive passage through HiTrap heparin and Superdex 75 columns (GE Healthcare). The nsp16/nsp10 complex was eluted as a single homogenous species in a final buffer containing 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... The protein was isolated by affinity chromatography (5 ml HisTrap, GE Healthcare Life Science, Chicago, USA) and desalted (5 ml HiTrap ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was loaded into a 5 mL HisTrap FF column charged with Ni2+ (GE Healthcare). The protein was eluted using a buffer containing 10 mM tris (pH 8.0) ...
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bioRxiv - Immunology 2022Quote: ... mAbs were harvested and purified 7 days after transfection using Protein G Sepharose columns (GE Healthcare). All purified recombinant mAbs were analyzed by SDS-PAGE under reducing conditions using NuPAGE Novex 4-12% Bis-Tris polyacrylamide gels and NuPAGE reducing agent (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... the protein samples were concentrated and injected into a Superdex 75 Prep Grade column (GE Healthcare) in a buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Genomics 2022Quote: ... All BPTF proteins were then further purified using anion exchange (Source 15Q, GE Healthcare Life Sciences) and size exchange chromatography (Superdex 75 ...
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bioRxiv - Physiology 2022Quote: ... The interactions between RS and proteins were measured using the BIAcore 3000 SPR system (GE Healthcare). The protein samples were diluted in HBS-EP buffer (0.01 M HEPES ...
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bioRxiv - Microbiology 2022Quote: ... The protein was affinity purified on a Ni2+-column (HiTrap IMAC HP, GE Healthcare Life Sciences). The eluted protein was cleaved with Rhinovirus 3C protease overnight and the cleaved complex was loaded onto a second Ni2+-column and the flow-through collected and further purified by size exclusion chromatography (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...
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bioRxiv - Microbiology 2022Quote: ... the protein was loaded into a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... We further purified the proteins through a 25 mL Superdex 200 preparatory-grade column (GE Healthcare) with 20 mM Na2HPO4 and 100 mM NaCl (pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for separation of proteins which were transferred to nitrocellulose blotting membrane (GE Healthcare, #10600002). Following washing with 0.05% Tris buffered saline-Tween (TBST) ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged fusion proteins were purified by Ni2+-Sepharose 6 fast flow (GE Healthcare, USA) affinity chromatography followed by size-exclusion chromatography.
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified via loading onto a 5 mL HisTrap HP column (GE Healthcare) and pooling the untagged elution ...
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bioRxiv - Biochemistry 2022Quote: ... all MIT proteins were purified by Superdex 75 gel filtration chromatography (GE Healthcare Life Sciences, USA) in 25 mM Tris (pH 7.2 at 25 °C) ...
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bioRxiv - Plant Biology 2021Quote: ... Then the protein complexes were loaded onto a Superdex 200 PC 3.2/10 column (GE Healthcare) at a flow rate of 0.05 ml/min ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred onto Amersham Hybond 0.2 PVDF blotting membranes (Cat# 10600021, GE Healthcare Life Sciences) in Tris glycine buffer (25 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... Total protein was quantified using the PlusOne 2D Quant Kit (GE Healthcare Europe GmbH, Freiburg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag fused proteins were purified by affinity chromatography column with GSTrap HP (GE Healthcare) and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated on a 10% SDS-PAGE and immobilized on a nitrocellulose membrane (GE Healthcare) using the Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... and POLGARF bound proteins were eluted by incubation with 1 μl of Prescission Protease (GE Healthcare) in PBS at 4°C overnight ...
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bioRxiv - Biophysics 2020Quote: ... the proteins were further purified by size exclusion chromatography (Superdex 200 16/60 HL, GE Healthcare) and eluted in 20 mM HEPES pH 7.6 at 8 °C ...
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bioRxiv - Immunology 2020Quote: ... The supernatant from pDNA transfected cells was purified using Protein G GraviTrap (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the antibodies secreted into the supernatant were collected and captured by protein A Sepharose (GE Healthcare). The bound antibodies were eluted and further purified by gel-filtration chromatography using a Superdex 200 High Performance column (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The target proteins were further purified through a Superdex200 10/300 gel filtration column (GE Healthcare) in a buffer containing 20 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... and the protein concentrations were determined using a 2-D Quant Kit (GE Healthcare; Piscataway, USA).
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bioRxiv - Immunology 2020Quote: ... Radiolabeled protein was purified by size exclusion chromatography (PD-10 column, GE Healthcare, 17-0851-01) and injected in the lateral tail vein of tumor-bearing mice ...
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bioRxiv - Bioengineering 2021Quote: ... mAbs were captured from the clarified green juice using MabSelect SuRe Protein A columns (GE Healthcare). Protein A Columns were equilibrated and washed with buffer containing 50 mm Tris ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further purified using an AKTA FPLC system with HiTrap-Phenyl-HP column (GE Healthcare) and Superdex 200 size exclusion chromatography ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted protein was dialysed by anion-exchange chromatography using a 1ml ResourceQ column (GE Healthcare) equilibrated with 20 mM potassium HEPES pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... The collected supernatant was purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... Ribosomes bound to an anti-HA antibody was purified using protein G magnetic beads (GE Healthcare). The RNA bound to ribosomes was purified with RNeasy Protect Mini Kit (QIAGEN ...
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bioRxiv - Cell Biology 2022Quote: ... The homogenates were precleared by incubation with protein G Sepharose (GE Healthcare, cat#17-0618-02) for 1 hour at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were further purified by size exclusion chromatography using a Superdex 200 HR column (GE Healthcare) and eluted in 20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Immunology 2022Quote: ... TAU-2212 in the supernatant was captured and purified by using protein A beads (GE healthcare), and was eluted by 0.1 M glycine at pH 3.2 ...
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bioRxiv - Immunology 2022Quote: ... the supernatants consisting of secreted antibodies were collected and trapped by protein G Sepharose (GE Healthcare). The bound antibodies on the Sepharose were eluted and concentrated using Centricon Plus-70 (051555 ...
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bioRxiv - Microbiology 2022Quote: ... The protein solution was then loaded onto an equilibrated 5 mL HiTrap-Q columns (GE Healthcare) using an ÄKTA pure 25 system (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... The eluted protein solution was then buffer exchanged using a PD-10 (GE Healthcare Life Sciences) desalting column.
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bioRxiv - Microbiology 2022Quote: ... The supernatants were filtered and purified by HiTrap Protein G HP antibody purification columns (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by Superdex75 or Superdex200 gel-filtration column (GE Healthcare, Piscataway, NJ). For crystallization experiments ...
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bioRxiv - Biophysics 2019Quote: ... The protein was purified by IEX on a HiPrep Q FF anion exchange column (GE Healthcare) and then further purified by SEC on a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... pH 7.0) and loaded to a 1-ml HiTrap Protein G Sepharose FF column (GE Healthcare). The column was washed with binding buffer until the UV280 absorbance dropped to a stable baseline ...
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bioRxiv - Biochemistry 2019Quote: ... Plasma proteins were separated by anion exchange chromatography using Resource Q column (GE Healthcare, 6 ml) connected to an ÄKTA Avant 25 system (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... Proteins were transferred to a nitrocellulose membrane (Amersham Protran 0.45 µm NC, GE Healthcare, Buckinghamshire, UK) using a semi-dry Western Blot system ...
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bioRxiv - Synthetic Biology 2019Quote: ... This protein was further purified using a size exclusion column (HiLoad16/600 Superdex 200pg, GE Healthcare) equilibrated with TED containing 100 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... His-tag removed protein was further purified by size-exclusion chromatography (S200 16/60; GE Healthcare) in buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... IP is carried out with clarified cell lysate pre-cleaned by protein G sepharose (GE Healthcare), then the cell lysate was incubated with anti-LRRC33 or control mouse IgG (2μg/ml ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were further purified using mono Q anion exchange chromatography and Superdex75 16/60 (GE Healthcare) size-exclusion chromatography ...