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Citations for GE Life Sciences :
2151 - 2196 of 2196 citations for Mouse Anti Hepatitis C Virus NS3 Antibody 1878 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Membranes were washed and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature and detected by chemiluminescence system (GE Healthcare Europe GmbH, Freiburg, Germany).
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bioRxiv - Immunology 2019Quote: ... IgG and IgM antibodies were purified from plasma samples by affinity chromatography using prepacked HiTrap™ Protein G HP columns (GE Healthcare, Wauwatosa, WI, USA) and HiTrap™ IgM Purification HP columns (GE Healthcare ...
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bioRxiv - Physiology 2020Quote: ... Immunoblotting was conducted with specific antibodies and protein bands were visualized using the enhanced chemiluminescence (ECL) Western Blotting Detection System (GE Healthcare, Chalfont St Giles, UK) or the Odyssey infrared detection system (LICOR).
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bioRxiv - Microbiology 2020Quote: ... IgG and IgA antibodies were depleted from plasma obtained from 25 recovered COVID-19 patient using Protein G HP Spintrap (GE Healthcare Life Sciences, Buckinghamshire, UK) and Peptide M / Agarose (InvivoGen ...
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bioRxiv - Immunology 2020Quote: ... and class II molecules were isolated by standard immunoaffinity purification using purified monoclonal antibody 2G11 [65] produced in-house and covalently linked to CNBr-activated Sepharose (GE Healthcare, Chalfont St Giles, UK). MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA ...
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bioRxiv - Cancer Biology 2022Quote: 1 mg lysate protein aliquots were used for immunoprecipitation with optimized amounts of the indicated antibodies and 20 μL of Protein A Sepharose beads (GE Healthcare, Chalfont St. Giles, UK) followed by SDS-Polyacrylamide (Biorad ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked and incubated with the antibody to detect neural Wiskott-Aldrich Syndrome protein (N-WASp; MilliporeSigma, Burlington, MA, USA) using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology) ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity was detected using a horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (IgG, 1:10,000, NA934 GE Healthcare). Tau13 and Tau5 immunoreactivity were detected using an anti-mouse IgG (1:10,000 ...
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bioRxiv - Microbiology 2019Quote: ... and RbmAb-phospho-histone H3 (Ser10/Thr11) were detected with donkey anti-rabbit IgG conjugated to HRP (GE Healthcare).
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bioRxiv - Immunology 2021Quote: Kinetics binding measurement for anti-spike mAbs in this study was performed using a Biacore T200 instrument (GE Healthcare). The system was flushed with filtered 1xHBS-P+ running buffer (0.01M HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times for 10 min in TBS-T and incubated with the secondary horse radish peroxidase (HRP) coupled antibody (GE Healthcare, NXA931V; in a 1:3000 dilution) for two hours under agitation at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The following antibodies have been conjugated to fluorochromes using commercially available kits according to the manufacturer’s instructions: anti-S100B has been conjugated to FITC (FLUKA) using Illustra NAP-5 columns (GE Healthcare), anti-GD2 (ch14:18 ...
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bioRxiv - Genomics 2020Quote: ... The membrane was incubated with 1:10,000 dilution of anti-rabbit IgG conjugated to horse radish peroxidase (HRP; GE Healthcare) diluted in 2.5% skimmed milk in TBS-T or anti-mouse IgG conjugated to HRP (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... the membrane was incubated with peroxidase-labeled donkey anti-rabbit IgG (#NA9340-1ML, Ge Healthcare Life Sciences, Chicago, IL, USA) with 5% nonfat dry milk for one hr ...
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bioRxiv - Immunology 2019Quote: ... were captured via their histidine tags onto CM5 chips pre-coupled with ~9000 reflective units anti-His Ab (GE Healthcare) using standard amine chemistry ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was washed three times with TBS containing 0.1% Tween 20 and incubated with horseradish peroxidase-linked anti-rabbit IgG (NA934, GE Healthcare) at a dilution of 1:10,000 for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by horseradish peroxidase-conjugated goat anti-rabbit (1:10,000) and detection on Super RX X-ray film (Fujifilm) using ECL Plus reagent (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... Western blot of the immunoprecipitated recombinant proteins was performed as described in the above section using anti-GST HRP (horseradish peroxidase) conjugate (1:10,000 Amersham GE Healthcare) for 60 minutes at room temperature under agitation ...
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bioRxiv - Physiology 2021Quote: ... The immunostaining was detected using horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) (GE Healthcare, Buckinghamshire, UK; dilution: 1:5000) or anti-mouse IgG (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... the membrane was washed with PBS-T and then incubated with HRP-conjugated anti-Rat IgG (1:1000; GE healthcare) for 1 h at RT ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was mixed with pre-equilibrated anti-GFP nanobody (GFPnb)-coupled cyanogen bromide-activated Sepharose beads (GE Healthcare) at 4 °C for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... and washed 10 minutes 3 times in PBST before being incubated with horseradish peroxidase-conjugated anti-rabbit IgG (GE Healthcare) diluted 1:2000 in the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Immune complexes were visualized using horseradish peroxidase-linked goat anti-rabbit immunoglobulin and enhanced chemiluminescence reagents (GE Healthcare, Tokyo, Japan).
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bioRxiv - Molecular Biology 2020Quote: ... Anti-Strep blots were imaged using an Amersham Imager 600 and Amersham ECL Prime Western Blotting Detection Reagent (GE Life Sciences).
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bioRxiv - Cell Biology 2021Quote: Membranes were then probed with goat anti-rabbit HRP (1:10,000, Licor) before being developed with SuperSignal West Femto (GE Life Sciences) and imaged on an iBright1500 (ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... Bands were detected using HRP-labelled anti-rabbit IgG and developed using an enhanced luminescence kit (GE Healthcare #GENA934, Watford, UK). Beta actin (Sigma #A5316 ...
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bioRxiv - Molecular Biology 2019Quote: ... The remainder of the lysates were incubated with polyclonal rabbit anti-GFP (EU2; Eusera) for 1 hour followed by Protein A-Sepharose beads (GE Healthcare) for another hour at 4° C ...
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bioRxiv - Immunology 2019Quote: ... washed with 1% BSA/PBS and incubated with Cy3-conjugated or FITC-conjugated goat anti-rabbit IgG (10 mg/ml; GE Healthcare) for 45 min respectively ...
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bioRxiv - Immunology 2021Quote: ... to prevent activation of the alternative complement pathway and complement consumption) and anti-FH mAb (35H9) on Hi-Trap HP-NHS columns (GE Healthcare). FH protein was purified from EDTA-plasma using an anti-FH mAb (35H9 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed and incubated with DAPI and anti-bleach reagents prior to imaging on a DeltaVision Elite Microscope (GE Healthcare). For all experiments ...
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bioRxiv - Immunology 2023Quote: ... the blot was washed three times every 5 min using 1X TBS-T before incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare) or goat-anti-mouse (GE health care ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purification was carried out using a high affinity anti-GFP nanobody covalently coupled to agarose beads (NHS-activated beads, GE Healthcare) in a 3:1 ratio (mg:ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidase was blocked (peroxide hydrogen at 3%) and slides were then incubated with anti-BrdU (BU-1; 1:100; GE Healthcare, RPN202) and phospho-histone H2AX (Ser139 ...
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bioRxiv - Immunology 2022Quote: ... anti-PD-L1 and LH02 were all purified by affinity chromatography using a protein A affinity column (GE Healthcare, Piscataway, NJ, USA) and analyzed in reducing condition on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Cell Biology 2022Quote: ... We performed immunoprecipitations with precoated anti-SRSF9 or rabbit normal IgG-protein A Sepharose beads (GE Healthcare, catalog number: 17-1279-02) for 2 □ hours at 4□°C ...
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bioRxiv - Immunology 2021Quote: ... 10μg/mL of C-terminal 6X-His tag containing recombinant SARS-CoV-2 Spike protein was captured at a flow rate of 10μL/minute for 180 seconds onto carboxymethylated (CM5) dextran sensor chips immobilised with anti-His mAb using EDC/NHS amine coupling kit (GE Healthcare, Australia). The sensor chips were equilibrated with running buffer [HEPES buffer (HBS-EP+ ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed in PBT and incubated with HRP conjugated-anti-rabbit IgG (GE Healthcare # NA9340, 1:5000 in blocking solution) at room temperature for 1 hour and washed twice in PBT ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibody solution was dumped and the membrane washed three times for 10 minutes each with 1xTBST before adding the polyclonal Anti-Rabbit IgG Peroxidase (GE Healthcare; NA9340V; 16908235) at a 1:5000 dilution in milk ...
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bioRxiv - Cell Biology 2019Quote: Binding affinities were measured using purified and immobilized GST-SH3Boi1 or GST-SH3Boi2 as ligands on an anti-GST chip on a Biacore X100 device (GE Healthcare, Freiburg, Germany). HBSEP buffer was used as running buffer in all experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... Bound Pnr 1-291 was identified by Western-blot (1:5000 rabbit anti-HA polyclonal) using an ECL kit (Amersham GE Healthcare Life Sciences) based on the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed and incubated for 30 min with horseradish peroxidase-conjugated goat anti-rabbit IgG (GE Healthcare Bio-Sciences, NJ, USA). Labeling was performed using an enhanced chemiluminescence (ECL ...
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bioRxiv - Developmental Biology 2020Quote: ... The membranes were washed with Tris buffered saline containing Tween 20 (AppliChem; TBST) and incubated with secondary anti-rabbit (GE Healthcare; Chicago, IL, US) or anti-mouse (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... IRDye® 680LT goat anti-rabbit IgG (H + L) (LiCor, cat. #926-6802, 1:10,000) and horseradish peroxidase (HRP)-conjugated goat anti-chicken IgG (GE Healthcare, cat. #AS09603, 1:10,000).
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bioRxiv - Biophysics 2022Quote: ... and anti–α-catenin shRNA (α-cat-KD) (both courtesy of G.D. Longmore, Washington University) were cultured in DMEM/F12 (GE Healthcare Life Sciences), supplemented with 5 % horse serum (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBST for 5 min and incubated for 1 h at room temperature with HRP-conjugated secondary anti-rabbit IgG (GE Healthcare, NA934, 1/2000) or anti-mouse IgG (Amersham ...