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Citations for GE Life Sciences :
2101 - 2150 of 4203 citations for Recombinant PvCSPAII CT Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare). The protein was dialyzed against 1X PBS overnight in the presence of 1:1000 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare) in 1XPBS pH 7.5 and eluted with 300 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was further purified using anion exchange chromatography (Mono Q 5/50 GL, GE healthcare) pre-equilibrated with ion exchange buffer A (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The flow-through fraction containing target proteins were collected and purified with Superdex 75 (GE Healthcare) chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... After electrophoresis the proteins were transferred to Nitrocellulose membranes (Amersham Protran Premium Nitrocellulose, GE Healthcare, #10600048) via wet transfer at a current of 45V for approximately 20 hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Eluted protein was further purified by size exclusion chromatography using a Superdex200 column (GE Life Sciences) in 25 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Diluted protein was subjected to anion exchange chromatography using a HiTrapQ HP column (GE Life Sciences) and eluted in 20 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 μm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine) ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 μm NC) at 90 V for 90 min on ice in transfer buffer (48 mM Tris-HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Biophysics 2020Quote: ... cells were pelleted and the supernatant was loaded onto a HiTrap Protein A column (GE Healthcare) following the manufacturers protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 35S-labelled proteins were monitored using autoradiography and visualised using a Typhoon 9400 scanner (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... The separated proteins were transferred to a 0.2 µm nitrocellulose membrane (Amersham Protran, GE Life Sciences) using wet transfer method for 2 h at 200 mA ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was visualized by Amersham enhanced chemiluminescence (ECL) Western Blotting Detection Reagent (GE Healthcare Life Sciences) and X-ray film (Fujifilm SuperRX) ...
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bioRxiv - Cancer Biology 2020Quote: ... Membrane-immobilized protein detection used ECL Western Blotting Detection Reagents (GE Healthcare RPN2106; Millipore Sigma GERPN2106).
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were separated by SDS-PAGE and were transferred onto PVDF membranes (GE Healthcare Life sciences) for immunoblot analysis ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the mixtures were purified with Dextrin SepharoseTM High Performance MBP-tagged protein purification resin (GE Healthcare). Ten micrograms of proteins from elutes was resolved on 12% SDS-PAGE gels and visualized with Coomassie blue staining ...
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bioRxiv - Biochemistry 2021Quote: ... and protein fractions were separated using a Superdex 200 10/300 column (GE Healthcare Life Sciences) with 50mM Tris (pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1mg of purified antibody was injected into the AKTA protein purification system (GE Healthcare Life Sciences) and protein fractions were separated using a Superdex 200 10/300 column (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μg of each protein extract were concentrated using the 2D Clean-up kit (GE Healthcare) and then resuspended in 30 mM Tris pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins (20-50 μM) were injected onto Superdex-200 Increase 10/300 column (GE Healthcare) for analysis at a flow rate of 0.5 mL min−1 ...
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bioRxiv - Cell Biology 2021Quote: ... His⨯6-tagged proteins in the supernatant were purified by Ni-NTA agarose beads (GE Healthcare). After extensive wash by a buffer containing 20 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted proteins were then separated by Superdex 200 Increase 10/300 GL column (GE Healthcare) that was equilibrated in size exclusive buffer (SEC ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were run on a 10 % SDS-PAGE and immobilized on a nitrocellulose membrane (GE Healthcare) using the Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... The rS1-Fc was protein A purified using MabSelect SuReLX resin (# 1754702) obtained from GE Healthcare.
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified from supernatants on HisTrap HP columns using an ÄKTApure (GE Healthcare) and resolved by gel filtration on a Superdex 200 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... The protein was purified by IEX on a HiPrep Q FF anion exchange column (GE Healthcare) and then further purified by SEC on a HiLoad 16/60 Superdex 75 prep grade column (GE Healthcare) ...
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bioRxiv - Systems Biology 2020Quote: ... Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare) to remove free His-tagged GST and TEV as well as remaining impurities ...
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bioRxiv - Molecular Biology 2020Quote: ... and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare). Finally ...
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bioRxiv - Neuroscience 2020Quote: ... The solubilized protein was then incubated with Sepharose-4B resin (GE Healthcare Life Sciences, Pittsburgh, PA) conjugated with monoclonal α-1D4 antibody (provided generously by Prof ...
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bioRxiv - Microbiology 2021Quote: ... and 10 µL of packed protein A sepharose beads (GE Healthcare cat. no. 17-0618-01) were then added ...
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bioRxiv - Microbiology 2021Quote: ... The target protein was further purified by size exclusion chromatography (Superdex 200 [16/60], GE Healthcare) in the buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, United Kingdom) in a wet tank unit (Bio-Rad ...
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Cytokinin-promoted secondary growth and nutrient storage in the perennial stem zone of Arabis alpinabioRxiv - Plant Biology 2020Quote: ... Total protein contents were assayed using the 2-D Quant Kit (GE Healthcare, Little Chalfont, UK), measuring OD480 in a microplate reader (Tecan Group ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were detected by Enhanced Chemiluminescence (ECL) using ECL Western Blotting Reagents (Amersham, GE Healthcare) or SuperSignal West Dura Extended Duration Substrate (Pierce).
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bioRxiv - Biochemistry 2021Quote: ... All eluted proteins were further purified on a SuperdexTM 75 10/300 GL column (GE Healthcare). The purified proteins were analyzed by SDS-PAGE and MALDI-TOF and concentrated using ultra-filtration through a 3 kDa cut-off Amicon (Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... The remaining portion was precleared using protein A Sepharose GL-4B beads (GE Healthcare, Piscataway, NJ) and incubated overnight with an antibody directed toward a specific histone modification ...
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bioRxiv - Biophysics 2020Quote: ... the reducing agents in the protein solution were removed with a PD10 desalting column (GE Healthcare), using a buffer based on 50 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... the protein was further purified using a size-exclusion column (Superdex, GE Healthcare, Chicago, Illinois, USA).
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bioRxiv - Biophysics 2020Quote: ... The proteins were purified by successive passage through HiTrap heparin and Superdex 75 columns (GE Healthcare). The nsp16/nsp10 complex was eluted as a single homogenous species in a final buffer containing 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... The protein was isolated by affinity chromatography (5 ml HisTrap, GE Healthcare Life Science, Chicago, USA) and desalted (5 ml HiTrap ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was loaded into a 5 mL HisTrap FF column charged with Ni2+ (GE Healthcare). The protein was eluted using a buffer containing 10 mM tris (pH 8.0) ...
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bioRxiv - Immunology 2022Quote: ... mAbs were harvested and purified 7 days after transfection using Protein G Sepharose columns (GE Healthcare). All purified recombinant mAbs were analyzed by SDS-PAGE under reducing conditions using NuPAGE Novex 4-12% Bis-Tris polyacrylamide gels and NuPAGE reducing agent (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... the protein samples were concentrated and injected into a Superdex 75 Prep Grade column (GE Healthcare) in a buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Genomics 2022Quote: ... All BPTF proteins were then further purified using anion exchange (Source 15Q, GE Healthcare Life Sciences) and size exchange chromatography (Superdex 75 ...
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bioRxiv - Physiology 2022Quote: ... The interactions between RS and proteins were measured using the BIAcore 3000 SPR system (GE Healthcare). The protein samples were diluted in HBS-EP buffer (0.01 M HEPES ...
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bioRxiv - Microbiology 2022Quote: ... The protein was affinity purified on a Ni2+-column (HiTrap IMAC HP, GE Healthcare Life Sciences). The eluted protein was cleaved with Rhinovirus 3C protease overnight and the cleaved complex was loaded onto a second Ni2+-column and the flow-through collected and further purified by size exclusion chromatography (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were mixed 2 h at 4°C with Protein-A-conjugated sepharose beads (GE Healthcare) and anti-Myc antibody (9B11 ...
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bioRxiv - Microbiology 2022Quote: ... the protein was loaded into a HiPrep 16/60 Sephacryl S-300 HR column (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... We further purified the proteins through a 25 mL Superdex 200 preparatory-grade column (GE Healthcare) with 20 mM Na2HPO4 and 100 mM NaCl (pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for separation of proteins which were transferred to nitrocellulose blotting membrane (GE Healthcare, #10600002). Following washing with 0.05% Tris buffered saline-Tween (TBST) ...