Labshake search
Citations for GE Life Sciences :
2101 - 2150 of 4264 citations for Recombinant Human IL1F10 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
-
bioRxiv - Immunology 2022Quote: ... Plates were subsequently washed 4 times with washing buffer and incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (GE Healthcare, NA933) at a 1:5000 dilution in PBS + 0.05% Tween-20 ...
-
bioRxiv - Microbiology 2019Quote: Human Custom ON-TARGET plus siKSRP or mimic miRs were resuspended in siRNA dilution buffer (Dharmacon, GE Healthcare, Chalfont-St-Giles, United Kingdom) to a 20 µM stock solution ...
-
bioRxiv - Immunology 2022Quote: Cells were isolated from human blood using density gradient centrifugation agent Ficoll®-Paque Premium (17-5442-02, GE Healthcare, SIGMA, Darmstadt, Germany). Monocytes were plated at 3×105 cells per well in plastic 24 well plates ...
-
bioRxiv - Immunology 2023Quote: ... freshly isolated mononuclear cells were separated from human blood by density centrifugation over Ficoll-Paque Plus (GE Healthcare, Chalfont St. Giles, United Kingdom). CD4+ T cells were subsequently purified by negative selection in an Automacs (Miltenyi Biotec ...
-
bioRxiv - Biophysics 2021Quote: ... lysed protein was purified by cation exchange using a 1 mL HiTrap SP HP (GE Healthcare) in protein buffer (50 mM Tris-HCl pH 7.0 ...
-
bioRxiv - Biophysics 2021Quote: ... The protein was purified from the lysate using Ni2+-NTA column of [KTA Pure (GE healthcare). For in vitro biotinylation of the polyprotein ...
-
bioRxiv - Biophysics 2020Quote: ... the protein was loaded onto a HiLoadTM 26/10 Q Sepharose high performance column (GE Healthcare), and eluted at ∼350 mM NaCl ...
-
bioRxiv - Molecular Biology 2020Quote: ... the proteins were purified by chromatography on a HiLoad 16/60 Superdex 200 column (GE Healthcare), in buffer containing 20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2019Quote: ... The proteins were blotted onto a nitrocellulose membrane (Amersham Protran 0.2 NC, GE Healthcare life sciences) for 1-2h at 300 mA ...
-
bioRxiv - Cell Biology 2020Quote: ... The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences). The beads were washed with binding buffer at least four times ...
-
bioRxiv - Cell Biology 2020Quote: ... Further purification of S-nitrosylated proteins were done using Thiopropyl Sepharose 6B bead (GE Healthcare# 170420001) in the presence of 50mM Na-ascorbate as a mild reducing agent ...
-
bioRxiv - Immunology 2021Quote: ... Protein bands were visualised using Amersham™ Imager 600 (GE Healthcare Life Sciences, Malborough, MA, USA).
-
bioRxiv - Microbiology 2021Quote: ... Protein-containing fractions were pooled for desalting through a 5 mL HiTrap Desalting column (GE Healthcare). Desalted protein was dialyzed into a storage buffer (50 mM NaH2PO4 pH 8.0 ...
-
bioRxiv - Developmental Biology 2020Quote: ... and GST-tagged target protein was purified using Glutathione Sepharose 4B (GE Healthcare, Marlborough, MA, USA) according to the manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2020Quote: ... Immuno-detected proteins were visualized by chemiluminescent photographic film exposure (ECL kit; GE Life Sciences, RPN2232) and digitally scanned using a GS-800 calibrated densitometer (Bio-Rad Laboratories) ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein was then purified by ion exchange chromatography (mono Q 10/100 GL, GE Healthcare), followed by SEC using a Superdex 200 increase 10/300 GL column (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
-
bioRxiv - Neuroscience 2021Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
-
bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
-
bioRxiv - Molecular Biology 2021Quote: ... the protein was subjected to size-exclusion chromatography on a HighLoad 16/60 Superdex75 (GE Healthcare).
-
bioRxiv - Molecular Biology 2020Quote: ... protein-RNA complexes were immobilized on Protran nitrocellulose membrane (0.45 μm pore size, Whatman, GE Healthcare) and unbound RNA immobilized on Hybond Nylon membrane (Amersham ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1500 µg protein extract containing GST-Gal1p was loaded on a GST SpinTrap column (GE Healthcare) and incubated for 1.5 hour at 4°C ...
-
bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
-
bioRxiv - Biophysics 2022Quote: ... The protein was further purified by anion-exchange chromatography on a Mono-Q column (GE healthcare). Expression and purification of RT600 was carried out in a similar way as with RT139A except that the 6x-His tag on p51 N-terminus was not cleaved ...
-
bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
-
bioRxiv - Cancer Biology 2019Quote: ... A suspension of Optison microbubbles (Perflutren Protein-Type A Microspheres Injectable Suspension, USP, GE Healthcare, USA) was infused intravenously (15 μl/minute ...
-
A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: ... Eluted protein was concentrated and subjected to a HiLoad 16/600 Superdex 75 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
-
bioRxiv - Biochemistry 2019Quote: ... protein supernatant was bound to a phenyl sepharose column (High Load FF 16/10, GE Healthcare). The column was washed with buffer C and protein eluted with 30% ethanol in water ...
-
bioRxiv - Biochemistry 2019Quote: ... The protein was fractionated on a HiLoad 16/600 Superdex 200 gel filtration column (GE Healthcare), eluting with SEC buffer (20 mM HEPES-KOH pH 7.5 ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein A Sepharose 4 Fast Flow and Con A Sepharose 4B were purchased from GE Healthcare.
-
bioRxiv - Molecular Biology 2019Quote: ... the reaction was added to 25 µL (50 µL 50% slurry) Protein G beads (GE Healthcare) pre-coupled with antibodies ...
-
bioRxiv - Plant Biology 2019Quote: ... and C-terminal 6xHis-tagged effector proteins were immobilised onto a NTA sensor chip (GE Healthcare). HMA protein was flowed over the immobilised effector at 30 µl/min (360 sec contact time and 180 sec dissociation time ...
-
bioRxiv - Molecular Biology 2019Quote: ... the protein was further purified through HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare) and eluted in buffer containing 20 mM Tris-Cl (pH 7.5) ...
-
Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... vector and GST-tagged or MBP-tagged proteins were captured using Glutathione Sepharose beads (GE Healthcare) or amylose resin (New England Biolabs) ...
-
bioRxiv - Immunology 2019Quote: ... The expected protein bands were detected using Image Quant LAS 4000 (GE Healthcare Life Sciences, USA). The relative abundance of the target protein (normalized to β-actin ...
-
bioRxiv - Microbiology 2019Quote: ... Buffer exchange of the purified protein was performed by using PD-10 desalting column (GE Healthcare).
-
bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by gel filtration in Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris ...
-
bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by gel filtration in Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris ...
-
bioRxiv - Plant Biology 2020Quote: ... Separated proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, United Kingdom) using a wet tank unit (Bio-Rad ...
-
bioRxiv - Cancer Biology 2019Quote: ... or a combination of the appropriate antibody and Protein A or G sepharose beads (GE Healthcare) were used to immunoprecipitate tagged or endogenous proteins from cleared lysates ...
-
bioRxiv - Biophysics 2019Quote: ... Eluted protein was further purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) and gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
-
bioRxiv - Cell Biology 2019Quote: ... IVT proteins were visualized by autoradiography using Typhoon™ FLA 9500 phosphorimager (GE Healthcare Life Sciences). Signal intensity was measured by ImageJ software after background subtraction ...
-
bioRxiv - Biochemistry 2020Quote: ... and were purified using HisTrap HP column and ÄKTA protein purification system (GE Healthcare Life Sciences). For crystallization ESK1 samples were further purified using Source 15Q anion exchange chromatography with 20 mM Tris pH 7.5 and 0 to 0.35 M NaCl gradient ...
-
bioRxiv - Pathology 2020Quote: ... Tau protein was further purified by FPLC using size exclusion Superdex75 and Superdex200 columns (GE Healthcare) in 1X PNE buffer (25 mM PIPES pH 7.0 ...
-
bioRxiv - Neuroscience 2019Quote: ... For dot blotting 50-100ng of protein was blotted on nitrocellulose (Hydrobond-C Extra, GE Healthcare) and these filters were not fixed or boiled ...
-
A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare). Finally ...
-
bioRxiv - Developmental Biology 2019Quote: ... His6-SUMO-tagged proteins were purified by affinity chromatography using a HisTrap HP column (GE Healthcare), followed by the cleavage of the His6-SUMO tag using Ulp1 protease ...
-
bioRxiv - Developmental Biology 2019Quote: ... The proteins were further purified by ion-exchange chromatography using a HiTrap S column (GE Healthcare) and eluted in a gradient of NaCl ...