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Citations for GE Life Sciences :
2101 - 2150 of 4305 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Protein expression was visualized with an Amersham ECL Western Blot System (GE Healthcare Life Sciences). β-Actin served as a loading control ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were subjected to anion exchange chromatography using the HiTrap™ Q column (GE Healthcare) using the ÄKTA™ start system (GE Healthcare) ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Protein bands were visualized using ECL Prime Western Blotting Detection Regent (GE Healthcare; Amersham, UK) and a light-capture cooled CCD camera system (ATTO Corp. ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins separated by 10 % Tris-glycine SDS–PAGE were transferred to nitrocellulose membranes (GE Healthcare) and processed for immunoblotting using the ECL Prime kit (GE Healthcare) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were purified using Glutathione Sepharose™ 4 Fast Flow (GE Healthcare #17-5132-03) and cleaved using 3C protease protein prior to reverse purification using glutathione beads followed by a HiLoad 16/60 Superdex 75 size exclusion chromatography.
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bioRxiv - Biochemistry 2024Quote: ... the proteins were purified with a Hiload 16/600 Superdex 200 pg column (GE Healthcare) preequilibrated with storage buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein expression levels were visualized with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Biochemistry 2024Quote: ... the protein elution was further purified by Superdex 200 Increase 10/300 GL (GE Healthcare).
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bioRxiv - Genetics 2024Quote: ... Proteins were detected by chemiluminescence using ECL SelectTM Western blotting Detection reagent (GE Healthcare, AmershamTM) and a Fujifilm LAS-3000 camera.
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bioRxiv - Immunology 2024Quote: ... Proteins were transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 mm NC) at 90V for 90 min on ice in transfer buffer (48mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein bands were visualized and imaged by an ImageQuant LAS 500 scanner (GE Healthcare, USA). After transfer ...
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bioRxiv - Biophysics 2021Quote: ... lysed protein was purified by cation exchange using a 1 mL HiTrap SP HP (GE Healthcare) in protein buffer (50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... The protein was purified from the lysate using Ni2+-NTA column of [KTA Pure (GE healthcare). For in vitro biotinylation of the polyprotein ...
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bioRxiv - Biophysics 2020Quote: ... the protein was loaded onto a HiLoadTM 26/10 Q Sepharose high performance column (GE Healthcare), and eluted at ∼350 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were purified by chromatography on a HiLoad 16/60 Superdex 200 column (GE Healthcare), in buffer containing 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were blotted onto a nitrocellulose membrane (Amersham Protran 0.2 NC, GE Healthcare life sciences) for 1-2h at 300 mA ...
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bioRxiv - Cell Biology 2020Quote: ... The purified soluble GST proteins were immobilized on glutathione sepharose 4B beads (GE Healthcare Life Sciences). The beads were washed with binding buffer at least four times ...
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bioRxiv - Cell Biology 2020Quote: ... Further purification of S-nitrosylated proteins were done using Thiopropyl Sepharose 6B bead (GE Healthcare# 170420001) in the presence of 50mM Na-ascorbate as a mild reducing agent ...
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bioRxiv - Immunology 2021Quote: ... Protein bands were visualised using Amersham™ Imager 600 (GE Healthcare Life Sciences, Malborough, MA, USA).
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bioRxiv - Microbiology 2021Quote: ... Protein-containing fractions were pooled for desalting through a 5 mL HiTrap Desalting column (GE Healthcare). Desalted protein was dialyzed into a storage buffer (50 mM NaH2PO4 pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein was purified by chromatography using a Nickel-loaded Hitrap Chelating HP column (GE Healthcare). Unbound material was extensively washed using Tris 20mM pH8 ...
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bioRxiv - Developmental Biology 2020Quote: ... and GST-tagged target protein was purified using Glutathione Sepharose 4B (GE Healthcare, Marlborough, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Immuno-detected proteins were visualized by chemiluminescent photographic film exposure (ECL kit; GE Life Sciences, RPN2232) and digitally scanned using a GS-800 calibrated densitometer (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was then purified by ion exchange chromatography (mono Q 10/100 GL, GE Healthcare), followed by SEC using a Superdex 200 increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was separated on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) with PBS as the running buffer ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were electrophoretically transferred onto the nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 100 V for 90 min on ice in the transfer buffer (48 mM Tris–HCl and 39 mM glycine supplemented with 20% methanol) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-fusion proteins were coupled to Glutathione Sepharose 4 Fast Flow (17-5132-01, GE Healthcare) for 2 h at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was subjected to size-exclusion chromatography on a HighLoad 16/60 Superdex75 (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... protein-RNA complexes were immobilized on Protran nitrocellulose membrane (0.45 μm pore size, Whatman, GE Healthcare) and unbound RNA immobilized on Hybond Nylon membrane (Amersham ...
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bioRxiv - Molecular Biology 2020Quote: ... 1500 µg protein extract containing GST-Gal1p was loaded on a GST SpinTrap column (GE Healthcare) and incubated for 1.5 hour at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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bioRxiv - Biophysics 2022Quote: ... The protein was further purified by anion-exchange chromatography on a Mono-Q column (GE healthcare). Expression and purification of RT600 was carried out in a similar way as with RT139A except that the 6x-His tag on p51 N-terminus was not cleaved ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... A suspension of Optison microbubbles (Perflutren Protein-Type A Microspheres Injectable Suspension, USP, GE Healthcare, USA) was infused intravenously (15 μl/minute ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: ... Eluted protein was concentrated and subjected to a HiLoad 16/600 Superdex 75 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... protein supernatant was bound to a phenyl sepharose column (High Load FF 16/10, GE Healthcare). The column was washed with buffer C and protein eluted with 30% ethanol in water ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was fractionated on a HiLoad 16/600 Superdex 200 gel filtration column (GE Healthcare), eluting with SEC buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein A Sepharose 4 Fast Flow and Con A Sepharose 4B were purchased from GE Healthcare.
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bioRxiv - Molecular Biology 2019Quote: ... the reaction was added to 25 µL (50 µL 50% slurry) Protein G beads (GE Healthcare) pre-coupled with antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... and C-terminal 6xHis-tagged effector proteins were immobilised onto a NTA sensor chip (GE Healthcare). HMA protein was flowed over the immobilised effector at 30 µl/min (360 sec contact time and 180 sec dissociation time ...
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bioRxiv - Molecular Biology 2019Quote: ... the protein was further purified through HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare) and eluted in buffer containing 20 mM Tris-Cl (pH 7.5) ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... vector and GST-tagged or MBP-tagged proteins were captured using Glutathione Sepharose beads (GE Healthcare) or amylose resin (New England Biolabs) ...
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bioRxiv - Immunology 2019Quote: ... The expected protein bands were detected using Image Quant LAS 4000 (GE Healthcare Life Sciences, USA). The relative abundance of the target protein (normalized to β-actin ...
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bioRxiv - Microbiology 2019Quote: ... Buffer exchange of the purified protein was performed by using PD-10 desalting column (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by gel filtration in Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... The proteins were further purified by gel filtration in Superdex 75 pg 16/600 (GE Healthcare) in 25 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: ... Separated proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (GE Healthcare, Little Chalfont, United Kingdom) using a wet tank unit (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... or a combination of the appropriate antibody and Protein A or G sepharose beads (GE Healthcare) were used to immunoprecipitate tagged or endogenous proteins from cleared lysates ...
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bioRxiv - Biophysics 2019Quote: ... Eluted protein was further purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences) and gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...