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Citations for GE Life Sciences :
2101 - 2150 of 4004 citations for Cow Cathepsin L1 CTSL Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: Protein samples were separated by 10% SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). After being blocked in 1 × PBST buffer containing 5% skimmed milk ...
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bioRxiv - Plant Biology 2023Quote: ... The protein concentration was determined with 2-D Quant Kit (GE Healthcare Life Sciences, Westborough, USA) using Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins of interest were revealed by chemiluminescence using the ECL WesternBlot detector kit (GE Healthcare) and the results were digitised in a UVITEC11Photo Imager (Ubitec Photoimager ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the supernatant was collected for protein purification with Ni-NTA columns (GE Healthcare, Uppsala, Sweden). The proteins were dialyzed with PBS at 4 °C and concentrated.
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bioRxiv - Molecular Biology 2023Quote: ... A small portion of the final proteins were run through the Superdex 200 columns (GE Healthcare) again for quality check.
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bioRxiv - Immunology 2023Quote: ... Samples were pre-cleared with 40 µl of protein G Sepharose beads (50% slurry, GE Healthcare) plus 5 μg Goat IgG (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... The protein was injected onto a Superdex-75 Increase 10/300 GL column (GE Healthcare 29148721) for size exclusion chromatography in SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Free copper was then removed by passing the protein through a HiTrap Desalting column (GE Healthcare). Prior to the SANS experiments ...
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bioRxiv - Biochemistry 2023Quote: ... and flowthrough containing phosphorylated proteins were purified over Hiload 16/600 Superdex 75pg column (GE Healthcare) pre-equilibrated with buffer containing 25mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: Anti-ASCT2 Ab was purified from the anti-sera using HiTrap Protein G HP (GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... All proteins were further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Separation of the labeled protein from residual dye was carried out using a PD10 (GE Healthcare) gel filtration column pre-equilibrated with hSSB2 storage buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Affinity purification of the protein was achieved using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer consisting of 100 mM tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... the protein was further purified by size-exclusion chromatography on a Sephacryl 300 column (GE Healthcare) in PBS.
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bioRxiv - Cell Biology 2023Quote: ... The protein was loaded onto a HiLoad™ 16/600 Superdex™ 200 column (GE Healthcare) equilibrated with a buffer containing 30 mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... 400 μL of 10% (v/v) protein A Sepharose CL-4B beads (GE Healthcare Life Sciences) in IP-2 buffer [50 Mm Tris pH 7.9 ...
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bioRxiv - Microbiology 2023Quote: ... The eluted protein was concentrated and applied to a Superdex increase S200 10/300 (GE Healthcare) size exclusion column equilibrated with 25 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare) were performed according to our published procedure (Ninagawa et al. ...
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bioRxiv - Genomics 2023Quote: ... 167 mM NaCl) and pre-cleared with protein-A-Sepharose beads (GE Healthcare 17-5280-02) for 30 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... and the proteins were then transferred onto nitrocellulose blotting membranes (NC) (10600001; GE Healthcare Life Science). The membranes were blocked with 5% non-fat dry milk and 0.1% Tween 20 for 30 min at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was fitered and protein purification was conducted using a Resource Q column (GE Healthcare) equilibrated in Buffer A ...
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bioRxiv - Cell Biology 2024Quote: ... Labelled protein bands were detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, USA) and ChemiDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: ... All proteins were further purified with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences) size-exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2024Quote: ... the protein was purified by size exclusion chromatography using a Sephadex 200 16/60 (GE Healthcare) column with the lysis buffer.
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bioRxiv - Biophysics 2024Quote: ... A calibration curve was produced for eluting species using known protein standards (GE Healthcare Life Sciences). The dimerisation-impaired D123P construct of Ire1-LD was used to assign the apparent molecular weight of eluted monomeric protein ...
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bioRxiv - Biophysics 2024Quote: ... The protein sample was passed through His Trap™ FF crude column (GE Healthcare, Piscataway, NJ). We expected the undigested protein to remain bound to the column ...
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bioRxiv - Cell Biology 2024Quote: ... GST or the GST fusion protein was purified using a glutathione Sepharose 4B column (GE Healthcare). GFP::RAB-5 protein from wild-type or usp-50(xd413 ...
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bioRxiv - Developmental Biology 2024Quote: ... The overexpressed proteins were bound to the washed Ni2+-chelating Sepharose (GE Healthcare, Pittsburgh, PA, USA). The suspension was mixed on a three-dimensional rotating mixer for 40 min at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The free dye was separated from labelled protein by a Hi-TRAP Desalting column (GE Healthcare). The final degree of labelling of FtsZ and ZapD was calculated from the molar absorption coefficients of the protein and the dye ...
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bioRxiv - Genetics 2024Quote: ... The proteins were electrophoresed on 10% SDS□PAGE gels and transferred to nitrocellulose membranes (GE Healthcare). The blots were blocked in 5% milk and incubated with primary antibodies overnight at 4 °C ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... proteins were electrophoretically transferred onto a nitrocellulose membrane (GE Healthcare, Amersham Protran Supported 0.45 µm NC) at 90 V for 90 min on ice in transfer buffer (48 mM Tris and 39 mM glycine supplemented with 20% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... IgG and IgM were captured by affinity chromatography using HiTrap Protein G High Performance column (GE Healthcare) and CaptureSelect IgM Affinity Matrix (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-ROC WT and GST-ROC K1347A mutant protein bound to Glutathione Sepharose 4B beads (GE Healthcare) were washed twice with Buffer A (20 mm Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein signal was detected using chemiluminescence with the ECL Prime Western Blotting Detection Reagent™ (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... 12 % and 15 % gels followed by protein transfer onto nitrocellulose membranes (GE Healthcare Life Sciences, 0.45 µm). For better visibility of endogenous HA-hATG8s membranes were boiled for 5 min in PBS after protein transfer ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were pre-cleared with a 20 μl bed volume of protein-A sepharose beads (GE Healthcare) by end-over-end rotation for 1 hr at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Labeled protein was further purified on a Superdex 200 increase 10/300 gel filtration column (GE Healthcare) equilibrated in buffer B + 100 mM potassium acetate (KOAc) ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein bands were visualized using an Amersham ECL Advance Western Blotting Detection Kit (GE Healthcare, Germany).
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bioRxiv - Microbiology 2020Quote: ... ECL (Amersham) chemiluminescence substrates were used for protein detection using an ImageQuant LAS 4000 system (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The translated proteins were resolved using SDS-PAGE and analyzed by phosphorimager analysis (Typhoon, GE life sciences).
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bioRxiv - Molecular Biology 2021Quote: ... The fusion proteins (GST-OsbZIP86 and MBP-OsSAPK10) were purified using Glutathione Sepharose 4B (GE Healthcare, USA) and Amylose Magnetic Beads (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Excess reducing agents were removed by running the protein solution through a PD-10 column (GE Healthcare) equilibrated with 20 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... Overexpressed recombinant proteins in supernatants were purified by AKTA start affinity chromatography system HiTrap Mabselect (GE Healthcare) according to the manufacturer’s instructions (S1 Fig).
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bioRxiv - Developmental Biology 2021Quote: ... Separated proteins were then wet transferred to a 0.45 μm nitrocellulose membrane (GE Healthcare Life Science, 10600003) in Towbin buffer (25mM Tris ...
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bioRxiv - Microbiology 2021Quote: Cell wall binding assays of recombinant PBP1 proteins fluorescently labelled with Cy2 bis- reactive dye (GE Healthcare) were performed as previously described (Bottomley et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... Bands corresponding to various proteins were detected by corresponding antibodies using ImageQuant LAS 4000 (GE Healthcare, Tokyo) and analyzed using ImageQuant software ...