Labshake search
Citations for GE Life Sciences :
2101 - 2150 of 2265 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Blots were rinsed twice and washed 4 x 5 minutes in 1X TBST and developed using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare RPN2232), according to the manufacturer’s suggestions ...
-
bioRxiv - Neuroscience 2021Quote: ... Lipofectamine 2000 and plasmidic DNA were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
-
bioRxiv - Neuroscience 2020Quote: ... Lipofectamine 2000 and siRNAs were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS, GE Healthcare Hyclone) and no antibiotics to ensure full transfection efficiency ...
-
bioRxiv - Genetics 2019Quote: ... 293T cell line was cultured in DMEM high-glucose medium with 5% HyClone® fetal bovine serum (GE Healthcare, Piscataway, NJ, USA) and were maintained at 37°C with 5% CO2.
-
bioRxiv - Biochemistry 2019Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
-
bioRxiv - Microbiology 2019Quote: ... Virus labeling was performed with Alexa Fluor™A647 succinimidyl ester in a 1:10 molar ratio and was then purified through Nap-5 desalting column (GE Healthcare). Labeled viruses were aliquoted and stored at −80°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Affinity-purified proteins were subjected to size-exclusion chromatography on a Superdex 200 10/300 column or on a Superose 6 5/150 column (GE Healthcare, Germany) in gel filtration buffer (50 mM Hepes pH 8.0 ...
-
bioRxiv - Neuroscience 2019Quote: ... α-syn was centrifuged at 12000g for 5 minutes and the supernatant loaded onto a Superdex™ 200 10/300 GL column (GE Healthcare). The column was eluted with PBS at a flow rate of 0.5 mL/minute ...
-
bioRxiv - Biochemistry 2021Quote: ... The eluted protein was cleaved with Precission™ protease for 2 hours at room temperature and applied to a 5 mL HiTrap Q HP column (GE Healthcare). Protein was eluted from the Q HP column with a 2M NaCl buffer in a 100 mL gradient ...
-
bioRxiv - Cell Biology 2021Quote: ... Figure 5F and Figure 5-figure supplement 1D were acquired and processed by iterative deconvolution using Soft-WoRx software (Applied Precision Ltd.). For Figure 1E ...
-
bioRxiv - Bioengineering 2021Quote: ... The dialyzed eluate was further passed through an equilibrated 5 mL heparin column (HiTrap™ Heparin HP) on a FPLC (AKTA pure, GE healthcare) and eluted using a linear NaCl gradient generated from heparin buffers A and B (40 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... that had been equilibrated in NiNTA A buffer followed by buffer exchange into SEC buffer using a 5 mL HiTrap® Desalting Column (GE Healthcare). Fractions containing protein of interest were pooled ...
-
A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: ... Purification was performed by combing a HisTrapFF column and analytical size-exclusion chromatographic Superdex™ 75 5/150 with an ÄKTAexplorer 100 (GE Healthcare). The human cDNA sequence for the HIF-1α-PAS-B domain was synthesized and subcloned into the prokaryotic expression vector pGEX-4T-1 (Amersham) ...
-
bioRxiv - Biochemistry 2020Quote: ... Both the LaGH13_20 and LaGH13_31B enzymes were purified by immobilized metal ion affinity chromatography on 5 ml HisTrap HP columns (GE Healthcare, Uppsala, Sweden) equilibrated with binding buffer (20 mM HEPES ...
-
bioRxiv - Biochemistry 2020Quote: ... diluted two-fold against fresh buffer B and then manually applied at room temperature to a 5 mL HiTrap Heparin cation exchange column (GE Healthcare # 17040701) that had been pre-equilibrated in buffer B ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein purification from precipitated cell-culture media was done on HisTrap HP 5 ml and HiLoad 16/600 Superdex 75 (GE Healthcare, USA) columns by the method described previously (Zahradník et al. ...
-
bioRxiv - Biophysics 2021Quote: ... Bacterial cells were disrupted by sonication and the soluble fraction was loaded on a 5 mL HiTrap Chelating HP column (GE Healthcare - USA) previously equilibrated with same buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2020Quote: ... The modified Cy3 labeled fragment was generated by labeling an amine-modified RNA containing a 5′ monophosphate ordered from Horizon Discovery with monoreactive Cy3 (GE Life Sciences) and the labeled RNA was purified by reverse-phase HPLC ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.5 % (v/v) glycerol by FPLC (ÄKTA explorer, Amersham Pharmacia Biotech) using a Superdex™ 200pg 16/60 FPLC column (GE Healthcare). Final protein fractions were analyzed by SDS-PAGE electrophoresis.
-
bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
-
bioRxiv - Biophysics 2022Quote: ... coli upec-117 CapW at 5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The combined eluants were incubated overnight at 4 °C with 5 mL of Glutathione-Sepharose 4B resin (GE Healthcare Bio-sciences AB). The beads were placed in a column and washed 3 times with 50 mL of Buffer B (480 mM NaCl ...
-
bioRxiv - Biochemistry 2022Quote: ... filtered through a 0.22 μm membrane filter and loaded onto a 5 ml HiTrap Heparin HP column (GE Healthcare, Chicago, Illinois, USA). The column was washed with 10 volumes of Buffer H (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... 10% glycerol at pH 8.2) and loaded onto a 5 mL prepacked Q-sepharose ion exchange column (GE Healthcare Life Sciences, MA) at 1 mL/min ...
-
bioRxiv - Microbiology 2020Quote: ... Crude extract was then prepared and subjected to purification by nickel-nitrilotriacetic acid (Ni2+−NTA) metal affinity chromatography using a 5 ml HisTrap™ HP column (29-0510-21; GE Healthcare). MenI was eluted at 200 mM imidazole ...
-
bioRxiv - Immunology 2020Quote: ... The supernatant was harvested 5 days post transfection and IgG was affinity purified using Protein A Sepharose (GE Healthcare, #17-5280-02). SDS-PAGE was used to confirm the purity of IgG and adequate cleavage of the P2A peptide ...
-
bioRxiv - Bioengineering 2021Quote: Several DSAs were performed to guide the intervention by injecting approximately 5-10 ml of a half-strength contrast agent (CA) (50% of Omnipaque 240 (GE Healthcare, USA) and 50% of a saline solution ...
-
bioRxiv - Microbiology 2021Quote: ... Fusion events were quantified by detecting GFP+ pixels after 18 hours incubation at 37°C 5% CO2 using Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10μm; GE Healthcare). Data was analyzed using the ImageQuant TL 8.2 image analysis software (GE Healthcare ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were then collected by vacuum filtration onto glass microfiber filters (grade GF/C, 125 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 50 mL centrifuge tubes (Sarstedt ...
-
bioRxiv - Genomics 2020Quote: ... mRNA was extracted from approximately 5 μg of the total RNA samples using Sera-Mag oligo-dT beads (GE Healthcare Life Sciences). The mRNA was fragmented and used to prepare sequencing libraries according to the Illumina TruSeq Stranded mRNA sample preparation kit ...
-
bioRxiv - Microbiology 2019Quote: ... FtsZ or ΔCTL were then purified from the lysate using anion exchange chromatography column (HiTrap Q HP 5 ml, GE Life Sciences), followed by ammonium sulfate precipitation (at 20% - 30% ammonium sulfate saturation) ...
-
bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Developmental Biology 2019Quote: ... The mixture was layered on 40 ml of 5% Percoll (cat no. 17-0891-02, GE Healthcare Bio-sciences AB, Uppsala, Sweden) in HBSS (cat no ...
-
bioRxiv - Cancer Biology 2020Quote: ... + RNA was first denatured by heating at 65°C for 5 minutes and then transferred to A cellulose nitrate membrane (Amersham, GE Healthcare, US) using A Bio-dot device (US Bio-RAD).The membranes were then uV-crosslinked ...
-
bioRxiv - Biochemistry 2020Quote: ... Nsp12 was then purified by Nickel-affinity chromatography followed by ion-exchange chromatography (using HisTrap FF and Capto HiResQ 5/50 column, respectively. GE Healthcare, USA). Nsp12 eluates within conductivity of 19-23 mS/cm were combined and injected onto a Superdex 200 increase 10/300 GL column (GE Healthcare ...
-
bioRxiv - Systems Biology 2021Quote: ... GTP-loaded protein was buffer exchanged into either NMR buffer or the GTPase assay buffer using NAP-5 Sephadex G-25 DNA Grade columns (GE Healthcare # 17085301). We were unable to obtain sufficient material for some mutants (H141E/I ...
-
bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 500 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA FPLC (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3 mL fractions ...
-
bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 1.2 column volumes of sizing buffer (25 mM Tris, pH 7.5, 250 mM NaCl, 2 mM DTT, 5% glycerol) using an ATKA Purifier (GE Healthcare, Piscataway, NJ). Protein was eluted isocratically in sizing buffer over 1.3 column volumes at a flow rate of 2 ml/min collecting 3-ml fractions ...
-
bioRxiv - Microbiology 2020Quote: ... Fusion events were quantified by detecting GFP+ pixels after 18 hours incubation at 37°C 5% CO2 using Amersham™ Typhoon™ Biomolecular Imager (channel Cy2; resolution 10μm; GE Healthcare). Data was analyzed using the ImageQuant TL 8.2 image analysis software (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... Rabbit sera were shipped to our laboratory where purification of IgG was performed by affinity chromatography using a 5-ml HiTrap protein A HP column following manufacturer’s instructions (GE Healthcare, Piscataway, NJ). Purified IgG protein concentration was determined by Nanodrop ND-1000 spectrophotometer ...
-
bioRxiv - Microbiology 2020Quote: ... The protein (5-6 ml) was further purified by FPLC over a 26/60 Superdex 200 pg size exclusion column (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: The supernatant fraction containing the rhGFAT2 protein (with or without tag) was subjected to purification using a Ni+2NTA affinity column (HisTrap HP 5 mL, GE Healthcare, USA). The column was equilibrated with 10 column volumes (CV ...
-
bioRxiv - Biochemistry 2020Quote: ... The dialyzed protein was diluted in buffer C to reach 50 mM NaCl immediately before loading to a Q-sepharose HP column (5 mL, GE Healthcare, USA), previously equilibrated with 10 CV of Buffer C ...
-
bioRxiv - Biochemistry 2021Quote: ... filtered (0.22 mm) and then applied to 5 mL HiTrap Chelating HP columns (GE Healthcare, protein from 6 L cells per column) charged with Ni2+ and equilibrated with 4 column volumes of buffer C (20 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... containing the cleaved protein, was concentrated (Vivaspin 20, MWCO 5 kDa) and loaded onto a Superdex 75 26/60 column (GE Life Sciences) equilibrated with SEC buffer (20 mM HEPES-KOH pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... after which it was passed through a 0.45-μm filter and loaded onto a pre-equilibrated 5-mL StrepTrap HP column (GE Healthcare, Chicago, IL) with bound SBP-FKBP12.6 fusion protein ...
-
bioRxiv - Biochemistry 2021Quote: ... Imidazole was added to the supernatant to final concentration of 10mM loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare) equilibrated in NiNTA A buffer (20 mM Tris pH 8.0 ...
-
bioRxiv - Biophysics 2020Quote: ... The supernatant was filtered using a 0.45 μm syringe filter and loaded onto a 5 mL Hi-Trap column (GE Healthcare Life Sciences) equilibrated with lysis buffer ...