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Citations for GE Life Sciences :
2051 - 2100 of 2887 citations for Tubulin Delta 1 TUBD1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT) and applied to Source 15S (meGFP) or Source15Q (scGFP) resin (GE Healthcare) equilibrated in 98.5% Buffer SA and 1.5 % Buffer SB (20 mM HEPES•NaOH ...
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bioRxiv - Microbiology 2022Quote: ... to be separated with two in-parallel 1-mL HisTrap FF crude columns (GE Healthcare). The column was washed with lysis buffer supplemented with 10 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 mM NaCl and 1 mM DTT using Superdex S200 10/300GL column (GE Healthcare).
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bioRxiv - Cell Biology 2024Quote: ... Grids were immediately blotted from the back side using Whatman filter paper #1 (GE Healthcare) within the automatic plunge freezer EM GP (Leica Microsystems) ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated for 1 hour with anti-rabbit (NA934, GE Healthcare; RRID: AB_772206) antibody diluted in TBS-T containing 5% milk ...
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bioRxiv - Immunology 2024Quote: ... at room temperature for 1 hour before visualization using the ECL Detection kit (GE Healthcare).
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bioRxiv - Cancer Biology 2024Quote: ... for 1 hour at room temperature and visualized using the ECL detection system (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... The dialyzed samples were subsequently loaded onto a 1 mL StrepTrap HP column (GE Healthcare), which was washed with 20 CV of binding buffer ...
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bioRxiv - Microbiology 2022Quote: ... and loaded onto an ion exchange column (HiTrap™ Q HP 1 mL, GE Healthcare) equilibrated with 0.05% LDAO ...
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bioRxiv - Microbiology 2022Quote: ... were loaded onto an ion exchange column (HiTrap™ Q HP 1 mL, GE Healthcare) equilibrated and washed in the same buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS, GE Healthcare, Chicago, IL) to remove nucleotide contaminants ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 1 h of incubation with protein A–Sepharose CL-4B beads (GE Healthcare). Immunoprecipitates were washed three times in lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... The soluble fraction was loaded onto a StrepTap HP 1 mL affinity column (GE Healthcare) at 4°C previously equilibrated with lysis buffer ...
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bioRxiv - Immunology 2023Quote: ... The protein solution was loaded on to a 1 ml Mono S column (GE Healthcare), eluted with a 20-bed volume linear gradient from 0 to 500 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... and cloning it into the EcoRI and SalI sites of pGEX-4T-1(GE Healthcare) in frame with glutathione S-transferase (GST ...
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bioRxiv - Microbiology 2024Quote: ... 300 mM imidazole) and further purified using a 1 ml Source 15Q column (GE Healthcare), running in buffer A (50 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... ShH10 particles were bound to a 1-mL HiTrap AVB Sepharose column (GE Healthcare, USA) and eluted with 50 mM glycine (pH 2.7 ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was loaded on a 1 ml HisTrap HP column (GE Healthcare, Pittsburgh, PA) and non-specifically bound proteins were removed by washing the column with 20 mM NaPO4 pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat AntiRat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1 hr at 24 °C with agitation ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat Anti-Rat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1hr at 24°C with agitation ...
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bioRxiv - Biophysics 2021Quote: ... lysed protein was purified by cation exchange using a 1 mL HiTrap SP HP (GE Healthcare) in protein buffer (50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Cell Biology 2020Quote: ... 2) the reaction mix was purified using anion exchange column (HiTrap QFF 1 mL; GE Healthcare); 3 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT buffer and was further purified on Heparin column HP Trap 5mL (GE Healthcare) using a 200-600 mM KCl gradient ...
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bioRxiv - Immunology 2019Quote: ... reaction mixture was applied to a 1 mL HiTrap KappaSelect affinity column (GE Healthcare Life Sciences). All affinity purification steps were carried out by connecting the affinity column to a peristaltic pump with flow rate of 1ml/min ...
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bioRxiv - Molecular Biology 2020Quote: ... Ion exchange experiments were performed on a Hitrap™ Q HP (1 mL) column (GE healthcare) with ion exchange buffer A and ion exchange buffer B (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... and hRIO2 (1-353) Δ(131-146) were further purified with a HiQ-Sepharose (GE Healthcare) chromatography ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA were extracted using the Illustra RNAspin kit and treated with DNase 1 (GE Healthcare). cDNA synthesis and taqman qPCR were performed as previously described [16] ...
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bioRxiv - Biochemistry 2019Quote: ... Reaction products were separated by ion-exchange chromatography (1 mL Hitrap Q HP, GE Life Sciences) using a gradient from 0.2 to 2 M ammonium acetate ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 hour at room temperature before being revealed using ECL Prime detection reagent (GE Healthcare) and chemiluminescence reading on a luminescent image analyzer (LAS-4000 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used 1% agarose gel electrophoresis and a NanoVue spectrophotometer (GE Healthcare Life Sciences, Buckinghamshire, UK). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared supernatant was loaded onto two HisTrap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... 25 mM HEPES/NaOH pH 8) into a 1 mL HiTrap Heparin HP column (GE Healthcare). This column was further washed with buffer NT.A ...
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bioRxiv - Neuroscience 2020Quote: ... sections for IHC were treated with streptavidin-horseradish peroxidase conjugate (GE Healthcare, Amersham, UK; 1:200) prior to visualization using metal enhanced chromagen ...
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bioRxiv - Biophysics 2021Quote: ... 1□mM DTT and 10% glycerol) using a PD MiniTrap G-25 sephadex columns (GE Healthcare) and the purified proteins were flash frozen in liquid N2 and stored at −80□°C ...
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bioRxiv - Biochemistry 2020Quote: ... The enzyme was purified using a 1 ml HisTrap FF column (GE Healthcare, Amersham, United Kingdom) with imidazole gradient elution and an S200 16/60 gel filtration column (Pharmacia Biotech ...
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bioRxiv - Biochemistry 2020Quote: ... The enzyme was purified using a 1 ml HisTrap FF column (GE Healthcare, Amersham, United Kingdom). The enzyme was dialysed into 20 mM Bis-Tris propane ...
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bioRxiv - Neuroscience 2021Quote: ... Tau13 and Tau5 immunoreactivity were detected using an anti-mouse IgG (1:10,000, NA931 GE Healthcare) diluted in 5% milk ...
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bioRxiv - Neuroscience 2021Quote: ... was cloned into the pGEX-6P-1 vector with an N-terminal GST tag (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was supplemented with 1 mM DTT and applied to Glutathione Sepharose 4B (GE Healthcare), and washed with 50 mM HEPES (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 1 mL IgG Sepharose beads suspension (6 Fast Flow, GE Healthcare) pre-rinsed with buffer for 20 ml lysate on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT using a S-200 10/300 analytical size exclusion chromatography column (GE Healthcare) connected in-line to mini-DAWN TREOS light scattering and Optilab T-rEX refractive index detectors (Wyatt Technology) ...
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bioRxiv - Plant Biology 2020Quote: ... the membranes were incubated with horseradish peroxidase–conjugated anti-rabbit IgG (1:10,000; NA934; GE healthcare) diluted with TBS-T for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare) to remove free His-tagged GST and TEV as well as remaining impurities ...
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bioRxiv - Plant Biology 2020Quote: ... separated in 1% [w/v] agarose gels and blotted onto Amersham Hybond-XL membranes (GE Healthcare) according to the supplier’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... The VHHs were purified from the solution using a 1 mL HisTrap HP column (GE Healthcare). To elute the bound VHHs a linear imidazole gradient starting from 20 mM and ending at 500 mM imidazole in binding buffer over a total volume of 20 mL was used ...
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bioRxiv - Microbiology 2019Quote: ... Eggs were collected on wet filter paper (Grade 1 filter paper, Whatman plc, GE healthcare, UK) and desiccated for 5–10 days before hatching in de-ionized water containing 1g/L bovine liver powder (MP Biomedicals ...
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bioRxiv - Molecular Biology 2020Quote: ... and POLGARF bound proteins were eluted by incubation with 1 μl of Prescission Protease (GE Healthcare) in PBS at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein was purified using a 1 ml HisTrap FF column (GE Healthcare, Amersham, United Kingdom) with imidazole gradient elution ...
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bioRxiv - Microbiology 2020Quote: ... Soluble extracts were mixed with 1 mL of glutathione agarose beads (catalogue number # 17075601, GE Healthcare) and incubated for 2 h at 4°C with gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... The mixture was loaded onto a Buffer-A-equilibrated 1 mL HiTrap QFF column (GE Healthcare). Then the column was washed sequentially by 10 column volumes of Buffer A ...