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Citations for GE Life Sciences :
2051 - 2100 of 2556 citations for Aldosterone Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... the array was washed 3 times for 5 minutes with 1X TBS-T solution incubated with the secondary antibody solution anti-rabbit HRP (Na934v, GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The MBP-Q44-HttEx1 protein was purified by fast protein liquid chromatography (FPLC) using a 5 ml HisTrap HP nickel column (GE Healthcare, Uppsala, Sweden) with 0.5 M imidazole gradient (SKU I5513-100G ...
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bioRxiv - Immunology 2023Quote: ... The products were filtered by 10 kDa-cutoff Amicon Ultra centrifugal filters to remove DNA and proteins and then analyzed by a Mono Q 5/50 GL column (GE Healthcare Life Sciences).
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bioRxiv - Bioengineering 2023Quote: ... Glass surfaces were activated by plasma coating for 10 minutes then adding Bind Silane solution (95% EtOH, 5% Acetic Acid, 0.3% Bind Silane; GE Healthcare: GE17-1330-01) for 10 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Cell suspensions were filtered through a 70 µm cell strainer, centrifuged (5 min, 500 g) and pellets were resuspended in DMEM containing 30% stock isotonic Percoll (SIP, GE Healthcare, Diegem, Belgium). Hereafter ...
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bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...
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bioRxiv - Biochemistry 2023Quote: Analytical size exclusion HPLC of purified complexes of Af1:7B8 and Af2:7B8 were carried out using a Superdex 200 5/150 GL column (GE Healthcare, Freiburg, Germany) equilibrated in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed trice with 1× DPBS for 5 min each and then washed thrice for 20 min using HyPureTM molecular biology grade deionized distilled (DDI) water (GE Healthcare Life Sciences). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... WT and C111S constructs were extracted from harvested cells by homogenization and soluble fractions loaded on a 5 ml HisTrap FF Crude column (GE Healthcare Life Sciences) equilibrated in binding buffer [20 mM Tris pH8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing the tagged domain were pooled and exchanged to the initial buffer containing 5 mM imidazole by using a PD10 desalting column (GE Healthcare Life Sciences). His-tagged TEV protease (0.1 mg/ml final concentration ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered soluble cell solution was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences) in Lysis Buffer ...
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bioRxiv - Immunology 2024Quote: ... IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare, Life Sciences) dependent on culture volume ...
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bioRxiv - Synthetic Biology 2024Quote: ... Clarified supernatant containing TetR was then purified using His-tag affinity chromatography with a Ni-NTA column (HisTrap FF 5 ml column, GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex HiLoad 26/600 200 pg column ...
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bioRxiv - Cell Biology 2020Quote: ... The mixture was incubated for 2 hours at RT and antibodies were isolated using illustra™ NAP-5 columns (GE Healthcare Life Sciences™), resulting in an average labeling rate between 0.8 and 1.
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bioRxiv - Biochemistry 2019Quote: ... The reacting agents were then separated from the now methylated proteins by size-exclusion chromatography using NapTM-5 columns according to the manufacturer’s instructions (GE Healthcare Life Sciences, Amersham, UK).
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bioRxiv - Immunology 2020Quote: HEK293T cells were grown at 37°C to approximately 80% confluence in 10 cm tissue culture dishes using DMEM with 5% Hyclone Fetal Clone II (GE Healthcare Lifesciences, Logan, UT) and 1X antibiotic and antimycotic (Gibco ...
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bioRxiv - Biochemistry 2021Quote: Nickel Immobilised Metal Ion Affinity chromatography (Ni2+-IMAC) for protein purification was conducted using a HisTrap® HP 5 mL column (GE Healthcare, Munich, Germany) coupled to a BioLogic DuoFlow™ Chromatography System (Bio-Rad ...
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bioRxiv - Biophysics 2021Quote: ... The culture medium containing the secreted ACE2 was subsequently loaded onto an ion-exchange column (HiTrap 5 ml Q FF anion exchange chromatography column; GE Healthcare, U. S. A.), and subsequently eluted by a linear salt gradient ranging between 10 and 1000 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... were purified by immobilized metal affinity chromatography (IMAC) using a HisTrap HP 5 mL column mounted on an Äkta Purifier System (GE Healthcare, Chicago, IL, USA). Fractions containing the His6-Sumo-peptide fusion in elution buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated in Buffer B ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three times with PBST for 5 min and incubated for 1 h at room temperature with HRP-conjugated secondary anti-rabbit IgG (GE Healthcare, NA934, 1/2000) or anti-mouse IgG (Amersham ...
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bioRxiv - Genomics 2020Quote: ... The mixture was left to react for 30 minutes protected from ambient light and the crude reaction mixture was passed through a NAP-5 column (GE Healthcare Life Sciences, 17085301) for collection and purification of the fluorophore-conjugated secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... Filtrates were then filtered gently by vacuum (<400 mbar under-pressure) over pre-combusted GF/A filters (> 5 h at 460°C; 47 mm, GE Healthcare Whatman, Buckinghamshire, UK), and finally over 0.22 µm pre-cleaned44 hydrophilic PVDF filters (<600 mbar under-pressure ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified in two steps by immobilized metal chelate affinity chromatography (IMAC) using 5 mL HisTrap FF columns (GE Healthcare, Chicago, Illinois, USA) as previously described ...
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bioRxiv - Biophysics 2020Quote: ... SEC was conducted at 4°C using a Superdex 200 5/150 column on an AKTA Pure System (GE Healthcare Life Sciences, Marlborough, MA) with a flow rate of 0.15 mL/min and 0.5 s exposures ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was dried for 30 min at 80° C in a drying oven before being blocked with 5% ECL advanced blocking reagent (GE Healthcare, Piscataway, NJ, USA) for one hour ...
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bioRxiv - Neuroscience 2024Quote: ... We separated equal amounts of protein on 5-20% SDS‒PAGE gels (Wako, Osaka, Japan) and transferred them to Hybond-P membranes (GE Healthcare, Piscataway, NJ, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Immunology 2024Quote: ... The wells of the plates were then washed 5 times with PBS-T and incubated with a 1:5000 dilution of anti-mouse IgG-HRP (GE healthcare cat. # NA931-1ml), or with a 1:100000 dilution of anti-ferret IgG-HRP (Abcam cat ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant desmin was purified under denaturing conditions (8 M urea) by ion exchange chromatography (IEC) using 5 mL HiTrap DEAE-FF columns (GE Healthcare, Chicago, IL, USA) and in a second step by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Molecular Biology 2023Quote: ... the partially purified proteins were further purified by chromatography through DEAE (flow through to remove nucleic acids at 0.3 M NaCl concentration) and Hi-Trap Heparin (GE HealthCare or Cytiva, 5 ml). The plasmid preparation and transformation protocols followed NEB recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc portion and uncut IgG was removed by flowing through a 1 mL or a 5 mL MabSelect SuRe column (GE Healthcare #11-0034-95) in PBS (Fab flows through the column) ...
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bioRxiv - Plant Biology 2021Quote: ... The immunoreaction was developed using the ECL Prime kit (Amersham, GE Healthcare) and was detected in the LAS-3000 Luminescent Image Analyzer (Fujifilm) ...
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bioRxiv - Cell Biology 2019Quote: ... total RNA was extracted with the illustra RNAspin Mini Kit (GE Healthcare). Complementary DNA was then synthesized using the SuperScript III First Stand Synthesis System (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... HRP activity was detected using the ECL detection system kit (GE Healthcare) and the Amersham Imager 680 (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Neuroscience 2019Quote: ... RNA was then extracted using an illustra RNAspin mini kit (GE healthcare). Input and eluate samples were used for cDNA synthesis using RevertAid Premium Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2019Quote: ... PCR products were cleaned with GFX Illustra DNA Purification Kit (GE Healthcare); standardized aliquots were sent to Macrogen (http://www.macrogen.com ...
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bioRxiv - Microbiology 2020Quote: ... Immunoblot signals were detected by Amersham ECL prime kit (GE Healthcare, RPN2232). Relative activation of MAPKs was analyzed by Image J software from three independent experiments.
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bioRxiv - Microbiology 2021Quote: ... The probe was produced with a Rediprime II labeling kit (GE Healthcare). Hybridization was performed as described by Dienst et al ...
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bioRxiv - Physiology 2021Quote: ... Membranes were visualised using enhanced chemiluminescence reagent (ECL Select Kit; GE HealthCare) and chemiluminescent signals were captured using the ChemiDoc image device (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... The Gel Filtration Calibration Kits (LMW, HMW) (GE Healthcare Life science, Germany) were utilized.
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bioRxiv - Cancer Biology 2020Quote: ... and enhanced chemiluminescence detection kit (Amersham, GE Healthcare Life Sciences, Cat# RPN2232) was used for the visualization of different proteins by a ChemiDoc MP Imaging System (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... exponentially dividing cells using Illustra TriplePrep kit (GE Healthcare 28-9425-44) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... chemiluminescence using ECLplus kit and detection either on ECL hyperfilm (GE Healthcare), on X-ray hyperfilm processor MI-5 (Medical Index ...
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bioRxiv - Cell Biology 2019Quote: ... immunoreactivity was visualised using ECL Prime detection kit (Cat. # RPN2232, GE Healthcare) and signal captured on a CCD-based camera (Syngene).
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bioRxiv - Molecular Biology 2020Quote: ... Detection was performed with an ECL prime immunoblotting detection kit (GE Healthcare). Chemiluminescence signals were captured by a luminoimage analyzer LAS-3000 (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... Total RNAs were isolated using the Illustra RNAspin Mini kit (GE Healthcare), and complementary DNAs (cDNAs ...
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bioRxiv - Microbiology 2020Quote: ... Detection was performed with an ECL plus immunoblotting detection kit (GE Healthcare).