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Citations for GE Life Sciences :
2001 - 2050 of 4443 citations for Mono N Butyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... z–stacks were collected using a UPLSAPO 100× oil (1.40NA) objective on a Delta Vision Core microscope (Image Solutions – Applied Precision, GE) attached to a CoolSNAP HQ2 CCD camera ...
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bioRxiv - Biochemistry 2019Quote: ... the beads were centrifuged and the supernatant was run over a HiPrep Sephacryl 16/60 S-100 HR column (GE Healthcare) in Crystal Buffer 1 (10 mM Tris-HCl pH 8.3 ...
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bioRxiv - Cancer Biology 2020Quote: Secreted form of the PD-L1 carrying C-terminal 10x His tag was purified using AKTA Start FPLC and Tricorn 5/100 column (both GE Healthcare) filled with HisPur Ni-NTA Resin (Thermo # 88223 ...
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bioRxiv - Synthetic Biology 2020Quote: ... The gel filtration was performed with a HiPrep 16/60 Sephacryl S-100 column on an AKTA chromatography system (GE Healthcare), and the size of the proteins in each eluted fraction were verified by SDS-PAGE.
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bioRxiv - Neuroscience 2020Quote: SEC-MALS experiments of 100 μM WT and all mutants were carried out by using a Superdex 200 10/300 column (GE Healthcare) in SEC buffer ...
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bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing target protein were pooled and exchanged back to 100% buffer A using a Sephadex desalting column (HiPrep 26/10 column, GE Healthcare). TEV cleavage was performed overnight at 23°C with 1 mM DTT ...
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bioRxiv - Biochemistry 2019Quote: 100 μl of protein sample was subjected to SEC at 293 K on a Superdex 200 (10/300) column (GE Healthcare) preequilibrated in 20 mM HEPES pH 8 ...
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bioRxiv - Biochemistry 2019Quote: For conjugation of pG to a SMCC-functionalized ODN an aliquot of pG was buffer exchanged to (100 mM Sodium Phosphate, 25 µM TCEP, pH 7) using a PD10 desalting column (GE Healthcare). Subsequently ...
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bioRxiv - Biochemistry 2021Quote: ... The complex was concentrated using an Amicon Ultra Centrifugal filter (MWCO, 100 kDa) and subjected to a Superose 6 Increase 10/300 GL column (GE Healthcare) that was pre-equilibrated with running buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: For crystallization trials the ATAD2 BRD was further purified by gel-filtration using a HiPrep 16/60 Sephacryl S-100 High Resolution column (GE Healthcare). The purified ATAD2 BRD 966-1112 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein containing elution fractions were concentrated on Amicon concentrator (100 kDa MWCO) and loaded a Superose 6 16/600 (GE Healthcare) pre-equilibrated in SEC buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Pathology 2021Quote: ... and 3500 V for 28 kVh (linear) with a Hoefer™ IEF 100 (M/s. GE Healthcare Life Sciences, Amersham, UK) at 20 °C ...
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bioRxiv - Biophysics 2020Quote: ... The resulting samples were concentrated by using 100 kDa centrifugal filter devices and subjected to size-exclusion chromatography (Superdex 200 16/60; GE Healthcare) with 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... supernatants containing lentiviral particles were filtered (0.45 μm) and concentrated to 200 μl using Vivaspin filters using Vivaspin filters with a 100 kDa molecular weight cutoff (GE Healthcare). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... Digested tissue was filtered through 100-μm cell strainers and colon and lung cells were further enriched by 40% Percoll (GE Healthcare) centrifugation ...
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bioRxiv - Genetics 2020Quote: 200 µg of antibodies R1 and R2 (against HSF2BP and MIDAP) were crosslinked to 100 ul of sepharose beads slurry (GE Healthcare). Testis extracts were prepared in 50mM Tris-HCl (pH8) ...
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bioRxiv - Biophysics 2019Quote: ... The protein solution was concentrated with a 100-kDa cut-off centricon (Milipore) and further purified by Superose-6 increase 10/300 column (GE Healthcare) using a buffer containing 50 mM Imidazole pH 6.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were concentrated using centrifugal concentrators (Amicon, 100 kDa) and polished by size exclusion chromatography (SEC, Superose 6 Increase, GE Healthcare) in buffer containing 25 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... ∼1 mg of protein was desalted into 100 mM sodium bicarbonate (NaHCO3) at pH 8.3 using a PD10 desalting column (GE Healthcare 17085101) according to manufacturer’s suggestions ...
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bioRxiv - Biophysics 2022Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer plus 0.03% digitonin ...
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bioRxiv - Biochemistry 2022Quote: SEC experiments were performed on 100 μL injections of 70 μM ScMlp11-325 with a Superdex 200 (10/300) GL column (GE Healthcare) at 0.5 mL min−1 at 25°C in 500 mM NaCl ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Each strain was subsequently transferred to a 100 mm Petri dish with an overlay of sterile autoclaved Hybond nylon membranes (84 mm) obtained from Amersham (GE Healthcare). The strains were then allowed to grow for 2-3 weeks on PDA media overlaid with this membrane ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion protein was eluted using binding buffer with 250 mM imidazole and then further purified using size-exclusion chromatography on a Hi load 16/60 Sephacryl S- 100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... To remove any residual impurities final step of size-exclusion chromatography on a Hi load 16/60 Sephacryl S-100 column (GE Healthcare) in the binding buffer with pH adjusted to 7.4 was employed ...
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bioRxiv - Molecular Biology 2023Quote: ... Column was washed with binding buffer and the proteins of interest were eluted with the binding buffer supplemented with 250 mM imidazole and applied to size exclusion chromatography column Hi load 16/60 Sephacryl S-100 column (GE Healthcare). Fractions of interest were pooled ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer with 1 mM MgCl2 and 0.03% digitonin ...
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Milipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare) running in lysis buffer with 0.03% digitonin ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated with a 100 kDa cutoff concentrator to 500 μL and loaded on a Superose 6 Increase 10/300 column (GE Healthcare) equilibrated with gel filtration buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: To create BAC transgenic mice 10 μg maxi-prepped BAC DNA (Nucleobond 100) was linearised by restriction digest (NotI) and purified by sepharose column purification (GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were then resuspended in 650 µL of dialysis buffer and 100 U of Thrombin (GE Healthcare, #GE27-0846-01), to a final volume of 1 mL ...
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bioRxiv - Developmental Biology 2023Quote: ... Each batch of imbibed seeds was equally distributed on 6 ½ MS plates containing 0.8 % plant agar and 0.5% sucrose (ca. 100 µl seeds per plate) topped with filter paper (Whatman Shark Skin Filter Paper, GE Healthcare #10347509). Seedlings were grown on the filter paper-topped MS agar plates in a growth chamber under long-day conditions ...
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bioRxiv - Biophysics 2023Quote: ... The eluted sample was concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and purified by Superose 6 Increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... developed with a salt gradient of 100 mM – 600 mM NaCl over 75ml followed by gel filtration on a Superdex 200 10/300 column (GE Healthcare) in buffer 20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to ∼2 µM for cryo-EM sample preparation.
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bioRxiv - Molecular Biology 2024Quote: ... the eluate from the Ni-column was diluted to 100 mM NaCl and further purified using a Q-column (GE Healthcare). Bound proteins were eluted by a linear gradient to 1 M NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... A 3.5-meter column with BC2L-A agarose beads was used for chromatographic separation at 100 µL/min using Äkta purifier (GE Healthcare) at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The purified receptor was subsequently concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and run on size-exclusion chromatography on a Superdex 200 10/300 column (GE Healthcare) pre-equilibrated with 30 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 2µM for cryo-EM sample preparation.
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bioRxiv - Biophysics 2024Quote: ... The fractions containing the protein were collected and concentrated using 100 kDa cutoff concentrator and loaded onto Superdex 200 10/300 GL column (GE Healthcare) using 20 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3C protease was removed by Ni-chelating sepharose and the heterotrimetric G-protein was further purified with MonoQ 10/100 GL column (GE Healthcare). Protein was bound to the column and washed in buffer A (20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... Analysis was done by western blotting using the primary antibodies directed against H3K36me3 (ab9050, 1:2000) or H3K36me2 (ab9049, 1:2500) and as secondary antibody the anti-rabbit HRP (Na934v, GE Healthcare, 1:5000). The signal was detected by chemiluminescence after the addition of Pierce™ ECL Western Blotting substrate.
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bioRxiv - Neuroscience 2019Quote: ... biotinylated anti-chicken (BA9010, 1:250, Vector Laboratories, CY3-streptavidin (1:500, GE Healthcare). Cell nuclei were counterstained with DAPI (0.2 mg/ml in PBS ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... and incubated overnight with primary antibodies (aFLAG 1:2,500, aHSP90 1:50,000, GE Healthcare) containing 0.01% Sodium azide (Sigma Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 then incubated with 1:50 (w/w) PreScission Protease (GE Healthcare) for 18 hrs at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... and the supernatant was loaded onto a 5 ml HisTrap HP column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... in 0.5 mL fractions using a pre-equilibrated PD-10 desalting column (GE Healthcare) and concentration was measured on a NanoDrop using a theoretical extinction coefficient of 10095 M-1cm-1 calculated from the amino acid sequences (all FapC variants contain 1 Trp ...
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bioRxiv - Microbiology 2019Quote: ... together with F185K at 20 μg/mL diluted in HBS-EP buffer (GE Healthcare) were immobilized on two flow-cells of a CM5 sensor chip by direct amine coupling to 3,000 response units (RU) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleared lysate was loaded onto a 5 mL HisTrap sepharose HP column (GE Healthcare) equilibrated with buffer A ...