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Citations for GE Life Sciences :
2001 - 2050 of 5304 citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The purified proteins were further dialyzed by using PD10 column (GE Healthcare), and concentrated using Amicon Ultra 30 K (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were purified using an Akta Purifier 10 chromatograph (GE Healthcare) and His Trap column ...
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bioRxiv - Physiology 2024Quote: ... Proteins were visualized after transfer onto a PVDF blotting membrane (GE Healthcare) and incubation with specific primary antibodies using horseradish peroxidase-conjugated secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Separated proteins were transferred to a nitrocellulose membrane (GE Healthcare, Freiburg, Germany) in transfer buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was applied onto a carboxymethyl sepharose fast flow column (GE Healthcare) and eluted with a sodium chloride gradient that contained 50 mM MES pH 6.5.
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein were incubated with Streptavidin beads (GE Healthcare, USA) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were immunoprecipitated by IgG Sepharose beads (GE Healthcare; 17-0969-01) (for Lid1-TAP ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was eluted by chromatography in 5-ml HisTrap (GE Healthcare) and digested with Smt3-specific protease Ulp1 (SUMO protease encoded in Rosetta pTB145) ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were then purified via binding to glutathione Sepharose (GE Healthcare) and washed ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors by Ficoll-Hypaque density gradient centrifugation (GE healthcare). Monocytes were isolated from PBMCs using magnetic CD14+ isolation beads (Miltenyi) ...
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bioRxiv - Immunology 2019Quote: PBMCs were isolated from whole human peripheral blood by Ficoll-Paque PLUS (17-1440-03, GE Healthcare, Pittsburgh, PA) density gradient centrifugation and cryopreserved prior to use ...
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bioRxiv - Genetics 2019Quote: ... Pools of siRNA against human NOVA2 gene and of Scramble siRNA were purchased from Dharmacon (GE Healthcare, Lafayette, CO).
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bioRxiv - Biochemistry 2022Quote: ... The complementary DNA of human ZIP4 (GenBank access number: BC062625) from Mammalian Gene Collection were purchased from GE Healthcare. The ZIP4 coding sequence was inserted into a modified pEGFP-N1 vector (Clontech ...
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bioRxiv - Cell Biology 2023Quote: ... Fibronectin was isolated from human plasma using Gelatin-Sepharose 4B (Cat No. 17-0956-01, GE Healthcare, Sydney, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats using Ficoll-Paque PLUS (GE Healthcare Bioscience). The PBMCs were cryopreserved in 40% heat-inactivated fetal bovine serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... The clarified lysate obtained after centrifugation (30 min at 20000 × g, 4°C) was first run on a streptavidin affinity column (StrepTrap® HP, GE Healthcare) equilibrated with septin 1 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences). Method for EMSA was modified from a previously published protocol (30) ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in 24 mL of RT 70% PercollTM (1.088 g/mL at 22 °C; GE Healthcare Life Sciences 17-0891-01). Aliquots of 8 mL cell/PercollTM suspension were overlayed with 4 mL HBSS and centrifuged at 400 xg for 30 minutes RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Double stranded Oligonucleotides were end-labeled with T4 polynucleotide kinase in the presence of [γ-32P]ATP and purified using G-25 columns (GE Healthcare). To remove secondary structures present in them ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Immunology 2019Quote: ... at a 1:1 (volume/volume) ratio and layered on top of a Ficoll-Paque-PLUS medium cushion (density 1.077 g/ml, GE Healthcare Life Sciences). Samples were centrifuged for 30 minutes at room temperature at 400 g with a soft stop ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were clarified by centrifugation (18,000×g for 60 min at 4°C) and incubated with glutathione Sepharose 4B (GE Healthcare, Uppsala, Sweden), applied to a gravity column ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the aqueous phase was used for size-exclusion chromatography by using a disposable PD SpinTrap G-25 column (GE Healthcare Life Sciences). The purified tRNALeu was then ethanol precipitated ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was cleared by centrifugation at 15’000×g for 1 h at 4°C and loaded on a His GraviTrap column (GE Healthcare Life Sciences), washed with 20 mM imidazole in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and immediately processed for isolation of peripheral blood mononuclear cells (PBMCs) by density gradient centrifugation using Ficoll-Paque Premium 1.077 g/mL (GE Healthcare, IL; 17544202) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: GST-fused bacterial expression plasmid for PAX9 was created by subcloning wild type and mutant (c.336C>G) PAX9 in BamHI and SalI site of pGEX4T1 vector (GE Healthcare, USA). E ...
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bioRxiv - Molecular Biology 2022Quote: The APAP stock solution (100 mM) was freshly prepared by dissolving 0.3 g APAP crystalline powder in 20 ml phosphate-buffered saline (PBS, GE Healthcare, Solingen, Germany) under heating (42°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Debris was pelleted for 10 min at 12000 x g and the supernatant incubated with PBS-washed glutathionesepharose 4FastFlow beads (GE Healthcare, 17075601) overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions were pooled and buffer-exchanged to sodium carbonate/hydrogen carbonate buffer pH 10 using a Sephadex G-25 column (GE Healthcare, UK). Recombinant protein was stored at −20 °C until required ...
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bioRxiv - Plant Biology 2020Quote: ... Reconstituted proteins were separated from the non-reconstituted ones utilizing the size-based column chromatography technique (Sephadex G-25M columns (PD-10 column, GE Healthcare, USA) (for detailed procedure see Roell et al. ...
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bioRxiv - Systems Biology 2021Quote: ... GTP-loaded protein was buffer exchanged into either NMR buffer or the GTPase assay buffer using NAP-5 Sephadex G-25 DNA Grade columns (GE Healthcare # 17085301). We were unable to obtain sufficient material for some mutants (H141E/I ...
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bioRxiv - Microbiology 2021Quote: ... Then the lysis was centrifuged at 15000 g for 30 min and the supernatant was loaded onto HisTrap HP column (GE Healthcare, America). The column was then washed with wash buffer (lysis buffer supplemented with 30 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The dried butanol fraction was then redissolved in 20mM of sodium phosphate buffer (pH 6) and desalted using a NAP10 desalting column prepacked with Sephadex G-25 resin (GE healthcare, USA). Finally ...
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bioRxiv - Molecular Biology 2019Quote: ... Equal amounts of protein (10-50 µg) from the total extract were separated on 8-10% SDS-polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Germany) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged in a Beckman 45Ti rotor at 100,000 x g for 30 min at 4 °C and the supernatant applied to a HiLoad 26/10 Q Sepharose column (GE Life Sciences) equilibrated in 90 % buffer A and 10% buffer B (buffer A ...
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bioRxiv - Genomics 2021Quote: ... G-25 sephadex™ G-50 DNA grade resin columns were used to remove unincorporated γ-32P ATP according to manufacturer’s instructions (illustra™ GE Healthcare). The purified strand was used for subsequent annealing with equal amount of complementary strand in 50 μL total volume ...
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bioRxiv - Neuroscience 2020Quote: ... 20–40 g) and placed on ice in Petri dishes containing 1X Hank’s Balanced Salt Solution (HBSS, SH30588.02, GE Healthcare Life Sciences, US). Ganglia were minced using surgical micro scissors and incubated in a papain solution (32 μL papain (27.3 U/mg ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified cell lysates were obtained by centrifugation at 12,000 x g for 15 min and subjected to affinity purification on a 5 mL Excel HisTrap affinity purification column (GE Life Sciences) equilibrated with IMAC-A buffer (20 mM sodium phosphate buffer pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by buffer exchange into G buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA) using desalting column (GE Healthcare; cat#17085101). To remove the GST tag ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Plant Biology 2023Quote: ... for 2 hours at 45°C and then desalted against 50 mM ammonium acetate using a PD MidiTrap G-10 column (size exclusion limit: 700 Da; GE Healthcare, Sweden) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The lysates were then centrifuged for 20 min at 45,000 g and 4 °C and the supernatants were filtered (0.45 μM) and loaded onto HisTrap HP columns (GE Healthcare, Uppsala, Sweden). Then ...
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bioRxiv - Immunology 2023Quote: ... to a ratio of 1 to 1 before being layered onto Ficoll Paque (1.077 g/ml; GE Healthcare Europe GmbH, Freiburg, Germany). After centrifugation (800 x g for 25 min) ...
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bioRxiv - Cell Biology 2024Quote: Purified LZTR1 proteins were centrifuged at 12,000×g for 10 min before being applied to an analytical Superose 6 10/300 SEC column (GE Healthcare Life Sciences) using a buffer containing 50 mM Tris/HCl (pH 7.4) ...
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bioRxiv - Biophysics 2021Quote: Proteins were purified on a Ni-NTA HiTrap HP column (GE Life Sciences). The purification tag was cleaved by the appropriate protease (see Construct Preparation ...
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bioRxiv - Biochemistry 2020Quote: ... The antibody ligands were captured on a Protein A chip (GE Life Sciences) and human CD45 D1-D4 (amino acids 194-578 ...
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bioRxiv - Bioengineering 2021Quote: ... on a Äkta Express fast protein liquid chromatography system (FPLC, GE Healthcare, USA). The lysate was loaded on the column which was afterwards washed with 12 column volumes Ni-wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Biophysics 2020Quote: ... hTRPC5 protein was further purified by size exclusion chromatography (Superose-6, GE Healthcare) in buffer containing TBS ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were further purified by size exclusion chromatography (Sephacryl 16/60, GE Healthcare) and stored in 50mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare). Chemiluminescence was detected with ChemiDoc Touch Imaging System (Bio-Rad Laboratories) ...