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Citations for GE Life Sciences :
1951 - 2000 of 2606 citations for 8 4 Chlorophenylthio guanosine 3' 5' cyclic Monophosphate triethylammonium salt since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 5% Glucose and the proteins purified by cation-exchange chromatography using a HiTrap SP High-Performance columns (GE Healthcare), followed by size exclusion chromatography using a HiLoad 16/600 Superdex 75pg column (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 mL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Biochemistry 2021Quote: ... The GST-tag and Thrombin protease were removed via heparin affinity chromatography (HiTrap Heparin 5 ml FF, GE Healthcare). Cas12a was eluted via an elution gradient with 1 M NaCl (20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein samples in buffer C were loaded on a heparin HP 5 mL column (GE Healthcare, Chicago, IL) and protein was eluted with buffer D (25mM Tris pH 7.6 ...
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bioRxiv - Plant Biology 2020Quote: ... The extracts were incubated with 5 μL anti-HA antibodies coupled with 30 μL Protein-A Sepharose (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Physiology 2021Quote: ... membranes were incubated for 5 min at room temperature with the Amersham ECL Select peroxidase substrate (RPN2235; GE healthcare), and imaged with the Fusion camera ...
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bioRxiv - Biophysics 2020Quote: ... The protein was purified from the supernatant of the lysate using a 5 ml HisTrap HP column (GE Healthcare). The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Secondary purification was performed by anion exchange chromatography using a HiTrap Q FF 5 mL prepacked column (GE Healthcare) installed on an Akta Start instrument (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... In order to purify MIF the lysate was first passed over a 5-mL CM-sepharose column (GE Healthcare), and after washing with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2019Quote: ... The water phase was loaded to a MonoQ 5/50 GL (1 ml) column (GE17-5166-01, GE Healthcare). ppGpp was eluted with a linear gradient from 0.5 mM to 600 mM LiCl in buffer C (25 mM Tris-HCl pH 8.3 ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through was then applied to a HiTrap Q HP 5 mL anion exchange chromatography column (GE Healthcare) attached to an ÄKTA Explorer 100 system (Amersham Pharmacia Biotech) ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was harvested on day 5 and ACE2 protein was purified using HisTrap FF column (GE Life Sciences), followed by desalting ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed three times in TBST and incubated for 5 minutes with a chemiluminescent reagent (GE Healthcare) to visualize immuno-reactive bands ...
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bioRxiv - Biochemistry 2021Quote: ... The flow-through was further purified by cation-exchange chromatography on a 5 mL HiTrap S column (GE Healthcare) with a salt gradient of 0 to 1 M NaCl in 1×PBS supplemented with 1 mM DTT ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were exposed to X-ray for 5-10 min using a biomolecular imager (ImageQuant LAS4000, GE Healthcare, USA). Relative cytokine content was determined after quantification of the dot intensity using ImageJ.
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bioRxiv - Biochemistry 2021Quote: ... and those containing GsI-IIC RT were pooled and loaded onto a 5-ml Heparin HP column (GE Healthcare) pre-equilibrated in Heparin Buffer A (20 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was filtered (0.45 µm) and then loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare) equilibrated with wash buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... All proteins were purified by Ni-NTA affinity chromatography using a 5 ml HisTrap column (GE Healthcare Life Sciences) equilibrated with 50 mM Tris-HCl pH 7.5 and 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Following centrifugation at 18000 ×g the soluble fraction was applied to a 5 ml HisTrap HP column (GE Healthcare). The column was washed and protein eluted along a gradient using 20 mM Tris pH8 ...
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bioRxiv - Immunology 2020Quote: ... 50 μL of complement component C5 at 31.6 μM (5.96 mg/ml) was injected onto a Superdex 200 Increase 5/150 column (GE Healthcare) at a flow rate of 0.35 ml/min ...
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bioRxiv - Molecular Biology 2021Quote: ... coli BL21 SI pRARE at 18 °C overnight and purified using a HisTrap FF 5-ml column (GE Healthcare) in standard buffers (50 mM HEPES-KOH pH 7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... the histones were purified from inclusion bodies under denaturing conditions on a 5 mL HiTrap SP FF (GE Healthcare) cation exchange column on a Next Generation Chromatograph (NGC ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Plant Biology 2022Quote: ... The filtrate was applied to a 5 mL HisTrap HP Nickel Sepharose™ affinity column (GE Healthcare Life Sciences) and washed with five column volumes of binding buffer (50 mM sodium phosphate buffer ...
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bioRxiv - Plant Biology 2022Quote: ... Dialyzed protein was then loaded onto a 5 ml HiTrap Q HP anion exchange column (GE Healthcare Life Sciences) and eluted with a gradient of 0-100% elution buffer (50 mM Tris-HCl (pH 8) ...
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bioRxiv - Plant Biology 2022Quote: ... The dialyzed samples were applied to a 5 ml HiTrap Q HP anion exchange column (GE Healthcare Life Sciences). The Q anion exchange column was eluted via linear gradients from 0% to 100% elution buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2022Quote: ... The soluble fraction was loaded onto a 5 mL Ni-NTA column connected to an ÄKTA pure (GE Healthcare) chromatography system ...
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bioRxiv - Neuroscience 2022Quote: ... The column was washed with 10 column volumes of Buffer A (10 mM HEPES, pH 7.3, 0.15 M NaCl, 5 mM imidazole) on an Aktapurifier (GE Healthcare) while collecting fractions ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lysate was then 0.45 μm filtered and applied to a 5 mL HisTrap HP column (GE Healthcare). The column was washed with five column volumes of wash buffer ...
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bioRxiv - Biophysics 2023Quote: ... filtered through 0.45 μm cut-off filters and loaded onto a HiTrap CaptoS 5 ml column (GE Healthcare, USA) for cation exchange ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µM Drp1 was injected onto a Superose 6 10/300GL column in an ÄKTApure FPLC system (GE Healthcare) connected in line with DAWN Heleos-II 18-angle MALS and Optilab T-rEX differential refractive index (dRI ...
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bioRxiv - Biochemistry 2023Quote: Dimer formation of non-farnesylated GBP1 was analyzed by analytical size-exclusion chromatography (Superdex 200 5/150, GE Healthcare) as described (Ince et al ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples (50 μl) were loaded on a Superdex 200 Increase 5/150 column (GE Healthcare, Chicago, Illinois, USA) pre-equilibrated with a 20 mM Tris-HCl buffer ...
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bioRxiv - Neuroscience 2023Quote: ... while α-Tubulin blots were placed in 5% milk PBST plus 1:10,000 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Cell Biology 2023Quote: ... PARP14 MD1 was additionally purified by ion exchange chromatography using a HiTrap™ 5 mL SP HP (GE Healthcare) equilibrated in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed 5 more times and the signal was detected by chemiluminescence using ECL Prime (GE Healthcare) on an Amersham Imager 600 detection system (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: ... Membranes were exposed to X-ray for 5-10 min using a biomolecular imager (ImageQuant LAS4000, GE Healthcare, USA). Relative cytokine content was determined after quantification of the dot intensity using ImageJ.
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (1-5 mg/ml) were loaded individually on a Superdex 200 Increase 10/300 column (GE Healthcare), and the elution profiles were obtained using a tandem of sequentially connected UV-Vis Prostar 335 (Varian ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered (0.45µm) and loaded onto a Ni2+-affinity column (HisTrap FF, GE Healthcare 5 mL column) equilibrated in low imidazole buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... supernatant filtered through 0.45 µm syringe filter and loaded onto a HiTrap Q HP column (5 ml, GE Healthcare) that was stacked on top of a SP column (5 ml ...
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bioRxiv - Bioengineering 2023Quote: ... The flowthrough sample was collected and loaded onto the pre-equilibrated HiTrap SP HP 5 mL column (GE Healthcare) with a flow rate of 2.5 mL/min ...
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bioRxiv - Biophysics 2023Quote: ... Data collection was carried out at 20 °C using a Superdex200 Increase 5/150 analytical SEC column (GE Healthcare) equilibrated in the appropriate buffers (see Table S3 ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins in the soluble fraction were purified by Ni-affinity column chromatography (HisTrap 5 ml column, GE Healthcare). The His6-tag was removed by thrombin digestion (1 μg of thrombin per mg of TelSi3 protein ...
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bioRxiv - Biochemistry 2024Quote: ... uncleaved protein as well as cleaved GST were removed by applying everything onto 5 ml SP column (GE Healthcare) equilibrated with 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... The TSP was then circulated through a pre-equilibrated (buffer A) Ni-NTA column (HisTrap, 5 ml, GE HealthCare) for 4 h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting filtrate was loaded onto 2 × 5 ml nickel–nitrilotriacetic acid (Ni–NTA) columns “HisTrapTM HP” (GE Healthcare) equilibrated with lysis buffer 2 ...