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Citations for GE Life Sciences :
1951 - 2000 of 2356 citations for 4 15 Oppositene 1 7 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The amplified PCR product was digested with BamHI and XhoI and ligated into the vector PGEx-6p-1 (GE Healthcare) digested with the same restriction enzymes ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated for 1 h with horseradish peroxidase (HRP)-conjugated secondary antibody (dilution 1:10,000) and developed with enhanced chemiluminescence (ECL) reagent (GE Healthcare). All western blots were visualized with a LAS4000 Imaging Station (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... rabbit serums from day 67 post-inoculation were purified using a 1 ml HiTrap NHS-Activated HP column (GE Healthcare) loaded with the corresponding antigen according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: His-SUMO-FacZ127-145 and GpsB1-75 were thawed from frozen aliquots and separately buffer-exchanged into an identical buffer (20 mM HEPES, pH 7.6, 500 mM NaCl, 1 mM DTT) using PD10 de-salting columns (GE Healthcare). Gel filtration samples were made by mixing GpsB1-75 at 90 μM with SUMO-FacZ127-145 at 30 μM in 500 μL reactions ...
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bioRxiv - Microbiology 2023Quote: The supernatant was filtered with a 0.22 μm filter and was loaded onto a pre-equilibrated HisTrap HP 1 ml column (GE Healthcare) at 0.5 ml/min with a peristaltic pump at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... vivax SAL-1 antigen was prepared from Aotus iRBCs purified by Percoll™(GE Healthcare Bio-Sciences AB, Uppsala, Sweden) cushion (47% ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The supernatant was collected by centrifugation at 25,000 g for 1 h and incubated with nickel Sepharose resin (GE Healthcare) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 mM DTT.) Cascade was further purified by size exclusion chromatography using a Superose 6 increase column (GE healthcare) in SEC buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Each clarified lysate was passed twice through a Ni2+-charged 1 ml HiTrap Chelating HP column (GE Healthcare Life Sciences) which had been equilibrated in Buffer TN (50 mM Tris pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (140,000 x g for 1 hr) and bound to a HisTrap FF column (GE Healthcare) in buffer A (50 mM Tris pH 8 ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was cleared by centrifugation (140,000 x g for 1 hr) and bound to a HisTrap FF column (GE Healthcare) in buffer A (50 mM Tris pH 7 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.15 M NaCl and 0.05% Tween 20 (TNT) at 46 °C for 1 h followed by a streptavidin-Cy5 (GE Healthcare) detection step ...
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bioRxiv - Biochemistry 2023Quote: ... were injected at flow rate of 0.4 ml min−1 to a Superdex 75 Increase 10/300 GL column (GE Healthcare) equilibrated with buffer containing 20 mM Tris-Cl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The BRCA1RING/BARD1RING and BRCA1RING/BARD1193 constructs were concentrated and further purified using a 120 mL Superdex 75 column on an AKTA FPLC system in SEC buffer (25 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 1 mM DTT; GE Healthcare). For the BRCA1RING/BARD1216 ...
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bioRxiv - Microbiology 2023Quote: ... the buffer was exchanged with 1% acetic acid at pH 3 and 0.02% sodium azide by using a HiPrep 26/10 desalting column (GE Healthcare). This ensured that the proteins were maintained in their monomeric form ...
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bioRxiv - Cell Biology 2023Quote: ... The column was run at a flow rate of 0.5 mL min-1 on an AKTAbasic liquid chromatography system (GE Healthcare) coupled to a miniDawn MALS detector and an Optilab refractive index detector (Wyatt Technology) ...
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bioRxiv - Bioengineering 2024Quote: The RSVF-V-1 and RSV90 Fab were purified by size exclusion chromatography using a Superdex200 26 600 (GE Healthcare) equilibrated in 10 mM HEPES pH 7.5 and 150 mM NaCl and concentrated to ∼20 mg/ml (Amicon Ultra-15 ...
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bioRxiv - Microbiology 2024Quote: ... non-fat dry milk in TBS-T for 1 h at room temperature in a rocking platform: anti-rabbit or anti-mouse IgG-HRP conjugated antibody from sheep (both 1:10,000 NA934 and NA931 GE Healthcare). Proteins were visualized using ECL or SuperSignal West Femto chemiluminescence reagents (Pierce ...
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bioRxiv - Microbiology 2024Quote: ... The solubilized protein suspension was ultracentrifuged at 100,000 x g for 1 hour before being loaded onto a 5 ml HisTrap HP column (GE HealthCare). The column was washed with a purification buffer supplemented with 20 mM imidazole ...
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bioRxiv - Plant Biology 2024Quote: ... RNA integrity was checked in agarose gel (1%) whereas the purity and composition were accessed by spectroscopy (NanoVue GE Healthcare). All the samples presented OD260/280 and OD260/230 > 1.8 and <2.1 ...
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bioRxiv - Cell Biology 2024Quote: ... at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare; cat. #RPN6302V) at room temperature for 45 min ...
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Exploring the druggability of the UEV domain of human TSG101 in search for broad-spectrum antiviralsbioRxiv - Molecular Biology 2024Quote: ... the supernatant was filtered using 0.45 µm syringe filters and loaded onto a 1 mL FPLC-GSTrap column (GE Healthcare) pre-equilibrated in Buffer A at a flow rate of 0.5 mL·min-1 ...
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bioRxiv - Biochemistry 2024Quote: ... 20 mM imidazole pH 7.5 was added prior to loading solubilized membranes on a pre-equilibrated 1 mL Ni-NTA column (GE Healthcare) at 1 mL min-1 ...
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bioRxiv - Biochemistry 2024Quote: ... Elution was performed with a gradient of 0.225–1.5 M NaCl in buffer K using Äkta FPLC (gradient length 30 mL, flow rate 1 mL/min, GE Healthcare). CF eluted at ∼825 mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... All proteins were purified by Ni-NTA affinity chromatography using a 1 mL His Trap column (GE Healthcare Life Science). The column was equilibrated with balance buffer (50 mM PBS pH 7.5 and 150 mM NaCl ...
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bioRxiv - Cell Biology 2024Quote: ... Samples at 1-1.5 mg/mL in 250 µL were injected into a Superdex 200 Increase 10/300 GL column (GE Healthcare) at a 0.5 ml/min flow rate ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-rabbit horseradish peroxidase-linked IgG and anti-mouse IgG, horseradish peroxidase linked antibodies (1:10,000; NA944V and NA931, respectively) were from GE Healthcare.
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed twice with PBS and incubated for 1 h with anti-mouse IgG horseradish peroxidase (HRP)-conjugated sheep antibody (GE Healthcare) diluted 1:4,000 in antibody dilution buffer ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the 4D11 mAb was carried out by affinity chromatography using a Protein A/Protein G GraviTrap 1-mL column (GE Healthcare), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... by injecting 50 µL of 8 µM protein in Protein Buffer onto a 1 mL HiTrap Heparin HP column (GE Healthcare) and eluting with a 10 CV linear gradient from 150 mM – 1 M NaCl in 20 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... suspended in buffer QA (10 mM Tris pH 8 and 1 mM EDTA) and loaded into a MonoQ 5/50 GL column (GE Healthcare). After a step-wise wash with 10% and 20% of buffer QB (Buffer QA supplemented with 2M NaCl) ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
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bioRxiv - Biophysics 2021Quote: ... The purification was done by purifying these two supernatants with 1 M NaCl containing Tris buffer using Hi-Trap Q-FF anion exchange column of ÄKTA Pure (GE healthcare). To maintain the oxidation activity of DsbA 0.3% H2O2 was added to the purified protein for overnight ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Immunoglobulins were purified against Protein A from 1 ml of rabbit serum using a Protein A HP SpinTrap (28-9031-32, GE Healthcare) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... the BglII–SalI fragment of HA-Catalase amplified by PCR from pUcD3/HA-HsCatalase (Otera and Fujiki, 2012) was cloned into the BamHI–SalI sites of pGEX6P-1 (GE Healthcare), thereby generating pGEX/HA-HsCatalase ...
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bioRxiv - Cell Biology 2020Quote: ... The complex was supplied with 2mM GTP and incubated at 4°C for 1 hour prior to purification by size exclusion chromatography on a HiLoad Superdex S75 16/600 column (GE Healthcare) equilibrated in 50mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were seeded the day before the transfection to reach 60-70% confluence and were then transfected with 25 nM of siRNA using the DharmaFECT 1 reagent (Dharmacon, GE Healthcare) following the Dharmacon siRNA transfection protocol ...
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bioRxiv - Immunology 2021Quote: ... media was diluted with an equal volume of Buffer A (500 mM NaCl, 20 mM sodium NaH2PO4, 20 mM imidazole) and loaded onto a 1 mL HisTrap column (GE Healthcare). The column was washed with Buffer A and the protein eluted with a gradient to Buffer B (500 mM NaCl ...
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bioRxiv - Neuroscience 2020Quote: The bacterial expression construct GST-MPP2-SH3-GK was generated by cloning the fragment encoding amino acids 220-552 (SH3GK) of mouse MPP2 into pGEX-6P-1 (GE Healthcare). GST-PSD-95-SH3GK was generated as described before [25] ...
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bioRxiv - Microbiology 2021Quote: 30 ml PBS flow-through plasmapheresis filters or peripheral blood diluted in PBS (1:3) were collected in 50 ml tubes and underlaid with Ficoll-Paque (GE Healthcare). PBMC-containing buffy coat was obtained after centrifugation for 30 min at room temperature at 1200 RCF ...
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bioRxiv - Microbiology 2020Quote: ... ParB-containing fractions were pooled and diluted to a conductivity of 16 mS/cm before being loaded onto a 1-mL Heparin HP column (GE Healthcare) that had been equilibrated with 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... which was added to the membrane for 1 min before the signal was detected using the ImageQuantTM LAS 4000 (GE Healthcare). Antibodies and dilutions used ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Purified NanR at 10 mg mL-1 (340 μM) was injected (70 μL) onto an inline Superdex S200 5/150 Increase (GE Healthcare), equilibrated with buffer C ...
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bioRxiv - Molecular Biology 2020Quote: ... the buffer was supplemented with 30 mM imidazole and an additional 100 mM NaCl and passed through a 1 ml Hi-Trap Chelating column (GE Healthcare) to capture the N-terminal fragment that was cleaved by the TEV protease ...
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bioRxiv - Biophysics 2022Quote: ... The individual complexes were incubated over ice for 1 hour and purified by size-exclusion chromatography (SEC) using a Superdex 200 10/300 GL column (GE healthcare) pre-equilibrated in a buffer containing 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... GST-Lc3b was constructed by cloning Lc3b into the BamHI and SalI sites of pGEX-4T-1 (GE Healthcare, IL, USA). The pac sequence was amplified from pBApo-EF1α Pur and cloned into pBApo-EF1α Neo ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µg of a 5’ amino-modified DNA oligonucleotide complementary to the target tRNA (IDT) was covalently coupled to a HiTrapTM NHS-activated HP column (1 mL, GE Healthcare). For isolation of full-length tRNAs ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR using the primers (5’-GGTTCCGCGTGGATCCATGTCTCATGCAGCCGAGCCA-3’ and 5’-GGAATTCCGGGGATCCTCAGGACTCCTCTTCAATGCTGA-3’) and cloned into BamHI site of pGEX-4T-1 expression vector (GE Healthcare) using In-Fusion HD Cloning System (Clontech) ...