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Citations for GE Life Sciences :
1951 - 2000 of 2233 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to less than 1 mL and was injected onto a Superdex 75 10/300 GL size exclusion column (GE Healthcare) equilibrated in gel filtration buffer (20 mM HEPES pH 7.7 ...
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bioRxiv - Biochemistry 2022Quote: ... of each protein were loaded at 5-8 mg mL−1 onto a pre-equilibrated Superdex 200 10/300 GL size-exclusion column (GE Healthcare) attached to multi-angle light scattering (DAWN HELEOS 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was separated by further centrifugation at 13 000 x g for 1 h at 4 °C and the supernatant was loaded onto a 5 mL HisTrap FF NiNTA column (GE Healthcare) equilibrated with lysis buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The resulting supernatant (∼1 ml) was incubated with 20 µl of dimethyl pimelimidate (DMP) cross - linked IgG Sepharose 6 Fast Flow (GE healthcare) resin for 2 h with gentle rotation at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... Fixed cells were rinsed three times in warm PBS and incubated 30 min with a blocking solution containing 1% BSA (Bovine Serum Albumine, GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Biophysics 2020Quote: ... Bound proteins were eluted using a linear gradient (10 column volumes) from 0 to 1 M NaCl in buffer A using an Äkta Purifier (GE Healthcare). Elution fractions (1.6 ml ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were centrifuged for 45 minutes at 100,000 x g and the supernatant was incubated for 1 hour with 5 mL Ni-NTA resin containing HisTrap FF column (GE Healthcare), which binds the gelsolin-actin complex through an N-terminal polyhistidine affinity tag on the gelsolin fragment ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were clarified by centrifugation at 50,000 × g for 1 h at 4 °C and the supernatant was used for affinity purification on HisTrap FF columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Fractions containing LPR1 (detected by immune blot analysis) were directly applied to cation exchange carboxymethyl-sepharose column (HiTrap CM FF, 1-ml, GE Healthcare) equilibrated with buffer B (20 mM Na2HPO4-NaH2PO4 ...
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bioRxiv - Plant Biology 2020Quote: ... an additional anion exchange (AIEX) step was performed prior to SEC by passing the protein over a 1 mL AIEX column (GE Healthcare). SnTox3 protein ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were then incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:2500, GE Healthcare Life Sciences) diluted in 2% non-fat milk prepared in PBS for 1 hour at RT followed by 3 washes with TBS containing 0.1% Tween-20 for 5 minutes each at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were separated on 12.5% (v/v) acrylamide gels of 25 cm x 21 cm x 1 mm using an Ettan DALTsix electrophoresis unit (GE Healthcare). The SDS-PAGE was first run at a current of 2 W/gel for 1 h and then at a constant current of 15 W/gel for 5 h ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated for 1 h at 4 °C and purified on a Superose 6 Increase 10/300 GL column (GE Healthcare) equilibrated with 25 mM sodium phosphate pH7.4 ...
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bioRxiv - Biochemistry 2020Quote: SEC-MALS analysis was performed by injecting 100 μl of purified protein at 1 mg/mL onto a Superdex 200 10/300 Increase GL column (GE Healthcare), pre-equilibrated in 20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Final gels were exposed to a storage phosphor screen for 1 hour and scanned using a Typhoon Imager 9410 (GE Healthcare). Band intensities were determined using the Fiji ImageJ software49 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were topped up to 1 ml with Buffer A and loaded onto a HiPrep 16/60 Sephacryl S-500 HR column (GE Healthcare). Buffer A was run at 1 ml/min and the flow-through was collected in 5 ml fractions ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation for 1 hour at 60000 x g and then loaded on a 5 mL Histrap FF column (GE Healthcare) equilibrated in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins (500 μl) at a concentration of 1 mg/ml were loaded on a Superdex 75 10/300 GL column (GE Healthcare) and eluted with HEPES buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... using pE-SUMO as an expression plasmid (86) and was isolated using a 1-mL HisTrap column and Äkta start (GE Healthcare). Before using the fusion protein for far western blotting ...
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bioRxiv - Microbiology 2020Quote: ... After incubating with horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG as a secondary antibody at 1:2000 dilution (GE Healthcare.), protein bands were visualized by chemiluminescence and the image was captured by ImageQuant LAS 4000 mini (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... 400 μl of purified protein at 1.4 mg·ml−1 was fractionated on a Superdex 200 10/300 column equilibrated in GF buffer using an AKTA purifier (GE Healthcare) at a flow rate of 0.5 ml·min−1 ...
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bioRxiv - Biochemistry 2021Quote: ... 1mM CaCl2 (pH 8.0)) at a flow rate of 1 mL/min on an ÄKTA-Pure instrument (GE Healthcare, Piscataway, NJ). The purified proteins were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions were analysed using SDS-PAGE and peak fractions were collected and further purified using a 1 ml MonoQ column (GE Healthcare). Sic1 was eluted with 20 column volumes (CV ...
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bioRxiv - Immunology 2020Quote: ... boiled and separated by gel electrophoresis in Tris-glycine SDS (TGS) buffer (20 mM Tris, 192 mM glycine, 1 % (w/v) SDS) and transferred to a nitrocellulose membrane (GE Healthcare) in Tris glycine (TG ...
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bioRxiv - Physiology 2020Quote: ... Blots were then washed in TBST and incubated for 1 h at 22-24°C with secondary HRP-conjugated antibodies (GE Healthcare) diluted 5000-fold in 5% skim milk in TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... Binding was detected by incubating the plates sequentially with either mouse anti-Histidine Tag antibody (MCA1396, Abd Serotec) followed by horseradish peroxidase (HRP)-linked anti-mouse IgG (1/2000, NXA931, GE Healthcare) or Streptavidin-HRP (554066 ...
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bioRxiv - Biochemistry 2020Quote: ... containing 0.05 M NaCl to a final concentration of 1 mg/ml was applied to a Superdex 10/300 column (GE Healthcare) run at a flow rate of 0.5 ml/min ...
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bioRxiv - Cancer Biology 2021Quote: ... or HRP-conjugated anti-mouse (CST, 7076; 1:1000) IgG secondary antibodies and visualized using ECL Prime Western Blotting Detection Reagent (GE Healthcare) and ImageQuant LAS 4000 Mini (FUJIFILM) ...
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bioRxiv - Biochemistry 2021Quote: The eluent from the Ni-NTA purification of the expression product of the pET-Duet-1 vector in BL21(DE3)pLysS* cells was loaded onto a 24 ml Superdex-200 10/300 GL (GE Healthcare) column on an AKTA Purifier-10 workstation ...
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bioRxiv - Biochemistry 2021Quote: ... and the digestion products were then loaded in 7 M urea containing 20% (wt/vol) polyacrylamide gels (acrylamide/bisacrylamide ratio 19:1) buffered with TBE and visualized using a Fluorescent Image Analyzer Typhoon (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... 1 μL of each homogenate was loaded onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA) and left to dry out at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 15 mM betamercaptoethanol, and 1 mM PMSF) and lysed using a microfludizer (Microfluidics, Inc.) followed by passage over a HisTrap column (GE Healthcare) and elution with a 20-400 mM imidazole gradient in lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of protein sample diluted to a concentration of between 0.02 mg/ml and 0.05 mg/ml was added to a grid and then manually blotted away with Whatman Grade 1 filter Paper (GE Healthcare). 3 µl of 0.75% Uranyl Formate was then added to the grid and blotted away ...
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bioRxiv - Biochemistry 2021Quote: ... Mer2 constructs were eluted with a lysis buffer containing 1 mM maltose and passed through a 6 mL ResourceQ column (GE Healthcare) equilibrated in 50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... the protein solution was again centrifuged at 15000 rpm for 1 hour and was loaded in an anion exchange column (Resource Q, GE healthcare) through an AKTA purifier FPLC system (Cytiva) ...
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bioRxiv - Plant Biology 2020Quote: ... DNA samples digested with restriction enzymes were separated in 1% agarose gels and blotted onto Hybond-XL nylon membranes (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... after which the lysate was pre-cleared for 1 h at 4 °C with 40 μl of equilibrated protein G-Sepharose beads (GE Healthcare). The day before ...
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bioRxiv - Biophysics 2021Quote: ... Purified TolC and colicin E1-T or -TR were mixed at a 1:2 molar ratio to ensure an excess of colicin to saturate TolC binding sites and incubated at room temperature for 1 hour before loading onto a Superdex 200 Increase 10/300 GL column (GE Healthcare). The protein was eluted with 1.5 column volumes into 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: A mixture of 1 µM GST-NDP52 and 100 nM GFP-ULK1 complex was incubated with 10 µL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... of RNA was resolved on a 1% agarose denaturing gel followed by blotting on a nylon membrane (Amersham Hybond-N+, GE Healthcare). A probe for mCherry or for 4× Broccoli was synthesized by PCR using the 4× Broccoli plasmid as template and the primers prW150-prW151 for Broccoli and prW459-prW460 for mCherry ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-SV40-NLS were cloned by in-frame insertion of the corresponding NFAT5 fragments and SV40-NLS into the C-terminus of GST of pGEX-4T-1 (GE Healthcare), respectively ...
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bioRxiv - Genomics 2021Quote: ... and AsNRDE-3 amino acids 45-246 (HQ611973) were fused in frame with GST using the Bam H1 site of pGEX-6P-1 (GE Healthcare), the proteins were expressed in E ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were cleared for 30 min at 12,500 × g at 4°C and the supernatant was collected and fractionated on a 1 ml Q-HiTrap column (GE Healthcare) equilibrated in 20 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The suspension was clarified by centrifugation at 30,000 x g (Sorval SLA 1500) at 4 °C for 30 min and loaded onto a 1 ml HisTrap HP column (GE Healthcare) on an ÄKTA purifier 10 system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... Two hundred and fifty micrograms of chromatin DNA were subjected to a 1 h preclearing with 50 μl of a 50% protein G-Sepharose (GE healthcare) bead slurry containing 1% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μl of each sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), and the dry membrane was incubated in NRP solution (0.01 g/ml KMnO4 in ddH2O ...
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bioRxiv - Biophysics 2023Quote: ... The solution was concentrated to ∼1 ml and went through gel filtration on Superdex 75 10/300 increase column (GE Healthcare) equilibrated with a buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... of GcapOCP2 or CrinOCP2 holoform samples (1 mg/ml in 100 μl) was carried out on a Superdex 200 Increase 10/300 column (GE Healthcare) connected to a tandem of consecutive detectors Prostar 335 (Varian ...