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Citations for GE Life Sciences :
1951 - 2000 of 3464 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The mass evaluated using UV as concentration source was 55.7 kDa for the 2 mg/ml sample.The Odinarchaeota samples were analysed by SEC-MALS (100 μl protein complex at 2 mg/ml) were passed over a Superdex 200 10/300 Increase GL column (GE Healthcare), in 20 mM Tris (pH 8.0) ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 mM DTT followed by size exclusion chromatography to remove any protein aggregates using a Superdex 75 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Cancer Biology 2020Quote: ... purified RNA was electrophoresed in non-denaturing 2% agarose TBE gel and then gel was blotted onto an Hybound-N nylon membrane (RPN303N, GE Healthcare) by semidry electroblotting in 0.5× TBE buffer for 2 hours at 200mA ...
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bioRxiv - Biophysics 2021Quote: ... 100-μl protein samples (at approximately 2 mg/ml) were loaded onto a Superdex 200 or 75 10/300 GL Increase size-exclusion chromatography column (GE Healthcare) in 20 mM Tris HCl ...
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bioRxiv - Biophysics 2020Quote: ... followed by 2 CV of 100% IEX buffer B using an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare). To determine the point of elution of aSyn from the chromatography column protein fractions which were collected and monitored on absorption at 280 nm were ran on a 4-12% Bis-Tris gel (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Peak fractions were pooled, diluted ~5 fold with buffer 0 (20 mM HEPES, 2 mM DTT) and loaded onto a Q column (GE Healthcare). Rad33 was then eluted from the Q column using a linear gradient of Q buffer A (20 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... Truncated mouse RANKL transcript (encoding amino acids 137-316 of mouse RANKL) was PCR amplified (forward primer: CACCCCCGGGCAGCGCTTCTCAGGAGCT, reversed primer: GAGACTCGAGTCAGTCTA TGTCCTGAAC) and then cloned into pGEX-5X-2 vector (GE Healthcare) between SmaI and XhoI sites ...
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bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Biophysics 2022Quote: Binding kinetics of ACE2 and SARS-CoV-2 RBDs were determined by surface plasmon resonance using a Biacore S200 (GE Healthcare). All experiments were performed in a running buffer composed of 10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: The binding kinetics of mAbs to SARS-CoV-2 Delta-RBD or Omicron-RBD monomer were analyzed using SPR (Biacore 8K; GE Healthcare). Specifically ...
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bioRxiv - Molecular Biology 2022Quote: ... the gel was washed with the wash buffer and the IF3-bound complexes were eluted by additional incubation of 2 h with the PreScission protease (GE Healthcare) (2 U/μl).
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bioRxiv - Microbiology 2022Quote: ... or His-NTV-AviTag (80 µl at 2 mg/ml) were injected on a Superdex 200 increase 10/300 GL column (GE Healthcare), equilibrated at 4°C with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The obtained pellet was resuspended in 2 mL of washing buffer and loaded on top of a 28% (v/v) Percoll (GE Healthcare) continuous gradient with a 0% to 4% (w/v ...
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Biochemistry 2022Quote: Antibody binding to SARS-CoV-2 spike was assessed using SPR on a Biacore T-200 (Cytiva, MA, formerly GE Healthcare) with HBS buffer supplemented with 3 mM EDTA and 0.05% surfactant P-20 (HBS-EP+ ...
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bioRxiv - Neuroscience 2020Quote: T1-weighted images providing high anatomical detail were acquired on 2 GE 3T Discovery 750× scanners (GE Healthcare, Waukesha, WI, USA) with an 8-channel phased array head coil (scanner 1 N=336 ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots were taken every 30 minutes for a period of 2 hours and quenched in 3ml of 5% trichloroacetic acid (TCA) on Whatman-glass fiber filters (GE healthcare) and washed 3 times with 1ml ethanol to dry the filter ...
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bioRxiv - Biochemistry 2021Quote: The binding affinities of full IgG antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... 50-μl protein samples (at approximately 2 mg/ml) were loaded onto a Superdex 75 10/300 GL increase size-exclusion chromatography column (GE Healthcare) in 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... to a volume of 2 ml and applied to size exclusion chromatography (SEC; HiLoad 26/600 Superdex 200 pg, GE Healthcare), After SEC ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplified fragments of the NP(412-500) or shorter fragments from pCAGGS-NP were cloned into pGEX-6P-2 (GE Healthcare). All sequences of the cloned or mutated DNA regions of the plasmids were validated by Sanger DNA sequencing.
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bioRxiv - Immunology 2021Quote: One mg per sample of mouse anti-H-2 Kb/Db antibody (ATCC HB-51) was bound and cross-linked to Protein A beads (GE healthcare) using dimethyl pimelimidate (DMP ...
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bioRxiv - Physiology 2020Quote: ... and incubated lysate equivalent to 2 mg of protein with 50 μL Streptavidin Sepharose High-Performance beads (GE Healthcare, IL, USA) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 3D widefield datasets were acquired using a 100x 1.4NA Uplan S Apochromat objective on a DeltaVision 2 Ultra microscope equipped with 7-Color SSI module and sCMOS camera and controlled by Acquire Ultra acquisition software (GE Healthcare), computationally deconvolved using the enhanced ratio constrained iterative deconvolution algorithm ...
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bioRxiv - Biochemistry 2020Quote: ... Nap1 containing fractions were concentrated to 2 mg/mL and loaded on a Superdex 200 10/300 GL gel-filtration column (GE Healthcare) pre-equilibrated with 25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... fluorescently labeled R18-HRSV or culture supernatants of HEp-2 were separated from excess R18 by a separation column (PD-10 Desalting Columns GE Healthcare). HRSV-R18 or culture supernatants of HEp-2 were incubated in suspension with A3.01 and HEp-2 cells at 4°C for 1 h to allow virus attachment ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplified products were separated by electrophoresis on a 2% agarose gel and quantified by using Image Quant TL analysis software (GE Healthcare). Quantitative real-time PCR was performed on a TP-850 Real-Time PCR Detection System (TAKARA Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length nsp1 was synthesized as a gene fragment from Integrated DNA technologies (IDT) and cloned into the EcoR1 restriction site of pGEX-6P-2 (GE healthcare) using in-fusion cloning (TakaraBio) ...
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bioRxiv - Immunology 2020Quote: ... An equal volume of buffer (2 mM EDTA in PBS) and blood was mixed and carefully layered over 5ml Ficoll Hypaque solution (GE Healthcare). After centrifugation for 20 min at 900 g (with no break ...
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bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Bioengineering 2021Quote: ... Sonications (10-ms bursts applied at 2 Hz for 100 s) were applied coincident with an injection of the MB ultrasound contrast agent Optison (GE Healthcare, Little Chalfont ...
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bioRxiv - Cell Biology 2020Quote: ... and differentiation was induced in differentiation medium (DM) for hMB consisting of DMEM (Nacalai) with 2% horse serum (HS) (HyClone; GE Healthcare) and PS.
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bioRxiv - Cancer Biology 2022Quote: ... were resuspended in 500 μl IP buffer and incubated with the appropriate antibodies on ice for 2 h and then 30 μl protein-G-sepharose beads (GE Healthcare; equilibrated once in 10 ml water and three times in washing buffer ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were incubated for 2 h at room temperature and visualized on X-ray films using enhanced chemiluminescence detection kit (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... The remaining lysate supernatant was incubated for 2 h on a spinning wheel with 200 µl of high-Performance strepTactin Sepharose beads (#28-9355-99, GE healthcare). The beads were collected by centrifugation at 1600 g at 4°C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples (100 μl at 2 mg/ml) were resolved using a Superdex 200 10/300 analytical gel filtration column (GE Healthcare) running at 0.5 ml/min at 25 °C in 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... Concentrated FhES (10 mg total protein) was fractionated (2 mL/fraction) by SEC on a Sephacryl S-200 HR 26/60 column (GE Healthcare) connected to an AKTA Start automated liquid chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2024Quote: IgGs were purified from ∼2 mL of plasma with an equal volume of Protein A Sepharose resin (GE Healthcare Life Sciences) and incubated with the resin for three days at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Samples were run for 2 h at 90 V and then scanned using a Typhoon FLA 9500 laser scanner (GE Healthcare) at a pixel size of 50 µm ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with blocking solution (2.5 % FBS, 200 mM glycine, 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Biochemistry 2023Quote: ... and 2 mM DTT) for 16 h before further purification by anion exchange chromatography on a HiTrap Q HP column (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... On day 2, cells were changed with fresh media containing RMPI1640 (Thermo, 21870) supplemented with 0.2% Hyclone FBS (GE Healthcare, SH30070.03) 100 ng/mL Activin A (R&D Systems ...
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bioRxiv - Microbiology 2023Quote: ... F045 and TriHSB.2 constructs contain C-terminal 6xHis tags and were purified by passage over a HisTrapFF column (GE Healthcare), followed by size exclusion chromatography using a Superdex 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Finally membranes were again washed three times with TBST and submerged in SuperSignal West Pico chemiluminescent substrate for 2 min before visualizing through ImageQuant LAS 4000 chemiluminescent Image analyzer (GE Healthcare). We used a variety of inhibitors including Torin 1 (inh-tor1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated for 2-h at room temperature with rabbit HRP-linked IgG (GE Healthcare, Chicago, IL, USA), and a chemiluminescence detection reagent (SuperSignal™ West Pico PLUS Chemiluminescent Substrate ...
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bioRxiv - Neuroscience 2023Quote: ... The PAGE gel was exposed to a phosphorimage screen for ∼2 hours and analyzed using a Typhoon imaging system (GE Healthcare). Band intensities corresponding to the uncleaved ribozymes and the two products of self-scission were analyzed using ImageQuant (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... M was concentrated to 2 ml using Vivaspin20 columns (SartoriusStedimBiotec) and purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50 mM NaH2PO4-Na2HPO4 ...