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Citations for GE Life Sciences :
151 - 194 of 194 citations for Tryptase delta Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Peptides were purified through a C18 prep column against an acetonitrile gradient on an AKTApure 25 FPLC (GE Healthcare) and confirmed by MALDI-TOF-MS (Bruker) ...
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bioRxiv - Microbiology 2022Quote: ... The solution containing the peptide was applied to a HiPrep SP FF 16/10 column (GE Healthcare Life Sciences) equilibrated with 20 mM sodium phosphate buffer at pH 3.9 ...
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bioRxiv - Plant Biology 2019Quote: ... pastoris and the peptide was purified from the culture medium using the Fast Protein Liquid Chromatography System (GE Healthcare) and Reverse Phase-High Performance Liquid Chromatography (RP-HPLC ...
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bioRxiv - Neuroscience 2022Quote: ... the Aβ42 peptide was recombinantly expressed in BL21*(DE3) pLysS Escherichia coli and purified with DEAE-Sepharose (GE Healthcare). To get rid of large Aβ42 aggregates ...
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bioRxiv - Plant Biology 2023Quote: ... the digested peptide filtrate was fractionated by strong cation exchange (SCX) chromatography using an AKTA Purifier system (GE Healthcare). The dried peptide mixture was reconstituted and acidified with 2 ml buffer A (10 mM KH2PO4 in 25%[v/v] of ACN ...
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bioRxiv - Cell Biology 2023Quote: ... The antibody was affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
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bioRxiv - Biochemistry 2023Quote: ... Crosslinked peptides were desalted and reconstituted with SEC buffer [30% (v/v) ACN in 0.1% (v/v) TFA] and fractionated using a Superdex Peptide PC 3.2/30 column (GE Healthcare). Collected fractions were analysed by liquid chromatography-coupled tandem mass spectrometry (MS/MS ...
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bioRxiv - Molecular Biology 2023Quote: Interactions between the DMC1 protein and the synthetic BRCA2 peptides were characterized using a VP-ITC Calorimeter (GE Healthcare). The peptides tested were wild type and F2406A variant of the PhePP (F2s6 ...
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bioRxiv - Developmental Biology 2023Quote: ... The antibodies were affinity-purified from the immunized serum with immobilized antigen peptides on CNBr-activated Sepharose (GE healthcare).
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked for 1 h at room temperature in TBS containing 4% Amersham ECL Prime Blocking Reagent (GE Healthcare) and 0.1% Tween 20 ...
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bioRxiv - Biophysics 2020Quote: ... Fixed cells were rinsed three times in warm PBS and incubated 30 min with a blocking solution containing 1% BSA (Bovine Serum Albumine, GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Biophysics 2023Quote: ... The fixed cells were rinsed three times in warm PBS and incubated for 30 min with a blocking solution containing 1% BSA (GE Healthcare) and 5% FBS in PBS ...
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bioRxiv - Biophysics 2021Quote: ... followed by buffer exchange in (50 mM HEPES, 150mM NaCl, pH 7.5) using a Superdex peptide size-exclusion column (GE Healthcare) to give the fluorescence labeled degron and Ub-degron.
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bioRxiv - Biochemistry 2019Quote: ... Crosslinked peptide fragments were enriched by SEC using a Superdex Peptide PC 3.2/30 column on an AKTA pure system (GE Healthcare) at a flow rate of 50 μL/min ...
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bioRxiv - Biochemistry 2019Quote: ... Resulting peptides after digestion were run in three independent Size-Exclusion Chromatography (SEC) runs on a Superdex Peptide 3.2/ 300 column (GE Healthcare) connected to an ÄKTAmicro FPLC system (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: Size exclusion chromatography was performed on a quaternary Agilent 1290 Infinity II UPLC system equipped with a SEC column (Superdex Peptide 3.2/300, GE healthcare). 40 μg of the eight protein mixture was loaded and separated at a flow rate of 0.05 ml/min with running buffer (30%ACN ...
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bioRxiv - Physiology 2022Quote: ... at a dilution of 1:2000 was done for 1 hour at RT in blocking buffer and detection was done using ECL detection kit (RPN2235, GE Healthcare, USA). Images were taken by the ImageQuant LAS500 (GE healthcare ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... to a polyvinylidene difluoride membrane and blocked in TBST buffer (TBS with 0.1% (v/v) Tween-20) supplemented with 1% ECL Advance Blocking Agent (GE Healthcare, Waukesha, WI) for 40 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... plasma or CSF probes were incubated in 25 μl for 1 hour at room temperature (diluted with PBS containing 5% Amersham ECL Blocking Agent, GE Healthcare RNP2125), followed by additional washing steps and incubation with an anti-mouse secondary antibody conjugated with the MSD Sulfo-tag for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed in PBT and incubated with HRP conjugated-anti-rabbit IgG (GE Healthcare # NA9340, 1:5000 in blocking solution) at room temperature for 1 hour and washed twice in PBT ...
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bioRxiv - Biophysics 2021Quote: ... the FLAG-tagged Ubr1 was eluted with 1mg/mL FLAG peptide and further purified by a Superose 6 size-exclusion column (GE Healthcare) equilibrated in a buffer captaining 50 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... 3D11 Fab-NPNDx2 and 1210 Fab-NANP5 peptide complexes were purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare) and diluted to 25 µg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... peptides were further fractionated prior to LC-MS by reversed-phase HPLC using an Ettan ÄKTA micro HPLC system (GE Healthcare), as described elsewhere98.
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bioRxiv - Biophysics 2019Quote: ... Sample was further purified from free labelled peptide and Sortase by size-exclusion chromatography using a Hi/Load 16/600 Superdex 75 column (GE Healthcare) previously equilibrated with ZapA storage buffer ...
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bioRxiv - Biophysics 2019Quote: ... The dissolved peptide was then fractionated on a Superdex-75 HR 10/30 size exclusion chromatography (SEC) column (GE Life Sciences) on a BIORAD FPLC system that was pre-equilibrated with 20mM Tris at pH 8.00 ...
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bioRxiv - Biochemistry 2021Quote: ... The separation of unreacted [35S]-fMet and each of the [35S]-fMet-peptide products was visualized by phosphorimaging and quantified using ImageQuant (GE Healthcare) and kinetic plots were fitted using Kaleidagraph (Synergy software).
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bioRxiv - Microbiology 2021Quote: ... The separation of unreacted [35S]-fMet from each of the [35S]-fMet-peptide products was visualized by phosphorimaging and quantified using ImageQuant (GE Healthcare) and kinetic plots were fitted using Kaleidagraph (Synergy software).
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bioRxiv - Neuroscience 2019Quote: SPR biosensor analysis was used to verify binding between peptides and the PDZ2 domain of FRMPD2 protein using a Biacore 8K instrument (GE Healthcare), as previously described(Zhang et al. ...
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bioRxiv - Microbiology 2019Quote: ... TolA was loaded into the cell and synthetic TolB peptide (residues 22-33; 2 mM) loaded into the syringe of an iTC200 microcalorimeter (Microcal/GE Healthcare). Titration consisted of 20 injections (1 x 0.4 μl ...
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bioRxiv - Biochemistry 2021Quote: SPR experiments were carried out to characterize the interaction between PKAc and substrate peptides using a Biacore T200 instrument (GE Healthcare). PKAc was immobilized via standard N-hydroxysuccinimide (NHS ...
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bioRxiv - Immunology 2020Quote: ... and thereafter were eluted with 20 mM reduced glutathione (recombinant GST or GST-fusion peptides) or incubated with PreScission Protease (GE Healthcare) overnight at 4 °C to remove the GST tag ...
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bioRxiv - Biophysics 2022Quote: ... MBP-AIM2 labeled with peptide-Alexa 488 was purified by size exclusion chromatography (SEC) using Superdex 200 increase 10/300 GL column (GE Healthcare) in a buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: Enzymatic degradations were monitored by analytical gel permeation chromatography using Superdex S200 10/300 and Superdex peptide 10/300 (GE Healthcare) columns mounted in series and connected to a high-performance liquid chromatography (HPLC ...
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bioRxiv - Microbiology 2024Quote: ... The active fraction (AGH1) was further purified to homogeneity by size exclusion chromatography using a Superdex Peptide HR 10/30 (Amersham Biosciences, GE Healthcare). Elution was performed under isocratic conditio ns with 30% ACN in acidified water at a flow rate of 0.5 mL/min ...
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bioRxiv - Biophysics 2021Quote: ... dye-peptide-MHC complexes in PLB were purified using a 10,000–molecular weight cutoff spin concentrator (Vivaspin 500, GE Healthcare, Pittsburgh, PA) and 1x TBS wash [TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleaved SPARC peptides were separated by 13.5% or 17% SDS PAGE and analyzed by immunoblotting or silver staining (GE Healthcare Life Sciences), respectively ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was dried for 30 min at 80° C in a drying oven before being blocked with 5% ECL advanced blocking reagent (GE Healthcare, Piscataway, NJ, USA) for one hour ...
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Plant Biology 2021Quote: The binding kinetics and affinities of MIK2ECD with SCOOP12 or SCOOP12SS/AA peptides were assessed on a Biacore T200 instrument (GE Healthcare, Pittsburgh, PA) with CM5 sensor chips ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for analyzing the binding of a synthetic GRP78-targeting peptide WDLAWMFRLPVG (GL Biochem Ltd, Shanghai, China) in Biacore X100 SPR system (GE Healthcare, NJ, USA). The system was firstly equilibrated with the running buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: The affinity of the sE protein for the pr peptide was measured by SPR using a Biacore T200 system (GE Healthcare Life Sciences) equilibrated at 25ºC ...
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bioRxiv - Immunology 2022Quote: ... Both mutants carry a carboxy-terminal c-myc-peptide extension and a poly-His peptide extension for anchoring in solid phase immunoassays and purification using nickel columns from GE Healthcare (Chicago, IL) as previously described and specified below (19 ...
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bioRxiv - Immunology 2023Quote: ... Correctly conformed HLA.A2-peptide complexes were purified using Ni2+-NTA agarose chromatography (HisTrap excel, 2 × 5 ml2 columns connected in series; GE Healthcare Life Sciences) followed by size-exclusion chromatography (Superdex 200 10/300 GL ...
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bioRxiv - Biophysics 2022Quote: ... The sample was then injected in an Äkta Purifier System equipped with a Superdex Peptide 10/300 GL column (GE Healthcare, Chicago, IL, USA) equilibrated with ultrapure water ...