Labshake search
Citations for GE Life Sciences :
151 - 200 of 2934 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl) and the lysate was batch binding with 300 μL Ni-NTA column (GE Healthcare, USA). After an extensive washing with buffer A ...
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bioRxiv - Molecular Biology 2019Quote: ... Curves were fitted to a 1:1 binding model using the accompanying Biacore evaluation software (Biacore/GE Healthcare).
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bioRxiv - Bioengineering 2020Quote: ... Data were fitted with 1:1 Langmuir binding kinetic model using the Biacore 8K analysis software (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...
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bioRxiv - Immunology 2022Quote: ... The resulting data were fit to a 1:1 binding model using the Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Bioengineering 2023Quote: ... Data were fit with 1:1 Langmuir binding model within the Biacore 8K analysis software (GE Healthcare #29310604).
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bioRxiv - Biochemistry 2022Quote: Ca2+ binding was characterized by ITC at 25 °C using a VP-ITC microcalorimeter (GE Healthcare, Northampton, MA) with a cell volume of 1.4619 ml in Na+ or K+ containing buffers (20 mM Tris pH 7.9 ...
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bioRxiv - Biochemistry 2023Quote: ... the binding affinity of fragments 1 – 4 and SlpB were evaluated with Biacore X100 (GE healthcare, Uppsala, Sweeden) with sensor chip protein G (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... SPR binding experiments were performed with a BIAcore 3000 instrument and analyzed with BIAevaluation software v4.1 (GE Healthcare). CM5 chips (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with secondary antibodies conjugated with horseradish peroxidase (HRP) diluted in 5% skim milk in TBS-T (1:10,000, GE healthcare). Labelled proteins were detected via enhanced chemiluminescence (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Microbiology 2023Quote: ... 17µl (∼ 5 µg) of each Cy-5-labelled (GE Healthcare) cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare ...
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bioRxiv - Physiology 2020Quote: ... non-essential amino acids (GE Healthcare HyClone Laboratories, Logan, UT), 1X Penicillin/Streptomycin and Gentamicin (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1% HyClone non-essential amino acids (GE Healthcare Life Sciences), and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Microbiology 2020Quote: ... The response data were fit to a 1:1 binding model using Biacore™ Insight evaluation software (GE Healthcare). For Fab competition experiments (Figure S8) ...
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bioRxiv - Microbiology 2019Quote: Direct binding of His6-SidI to SusF was assessed by SPR using a Biacore T-200 instrument (GE Healthcare) at 25 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). The equilibrium dissociation constants (binding affinity ...
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bioRxiv - Microbiology 2021Quote: ... Data were double reference-subtracted and fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... Sensorgrams were fitted using 1:1 binding model and kinetic constants determined using Biacore T200 Evaluation software (GE Healthcare).
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bioRxiv - Biophysics 2021Quote: The binding analysis of the substrate analog AMP-PNP was carried out in a Biacore 8K instrument (GE Healthcare) using an NTA Series S sensor chip (Cytiva) ...
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bioRxiv - Immunology 2022Quote: ... The resulting data were fitted to a 1:1 binding model using the Biacore 8K Evaluation software (GE Healthcare). To determine the levels of nanobody competition with the human ACE2 and VHH72 of known epitope specificity ...
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bioRxiv - Synthetic Biology 2019Quote: ... and regression curves were fitted based on a one-binding site model using the Microcal Origin software (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... gels were firmly attached to one of the two glass gel plates via binding silane (GE Healthcare, Uppsala, Sweden) whereas the opposite plate was coated with a releasing agent (Blueslick ...
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bioRxiv - Biochemistry 2020Quote: The binding affinity of GDAP1Δ319-358 and GDAP1Δ295-358 towards HA was measured using a MicroCal iTC200 calorimeter (GE Healthcare) in SEC buffer with 2% (v/v ...
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bioRxiv - Biophysics 2021Quote: SecA:lipid binding experiments were performed using L1 sensor chip on two-channel Biacore X100 instrument (GE Healthcare Life Sciences). DOPC-only liposomes lacking SecA interaction were employed in the reference channel ...
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bioRxiv - Cell Biology 2021Quote: Sensorgrams of lncEry-P5 binding to Wdr82 were measured by SPR technology using a Biacore 3000 instrument (GE Healthcare). The flowing-phase RNA lncEry-P5 was synthesized by in vitro transcription using a T7 RNA production system (Promega ...
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bioRxiv - Immunology 2021Quote: Kinetics binding measurement for anti-spike mAbs in this study was performed using a Biacore T200 instrument (GE Healthcare). The system was flushed with filtered 1xHBS-P+ running buffer (0.01M HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The first step was affinity purification on an MBP-binding dextrin Sepharose column (MPBTrap, GE Healthcare: 28-9187-78). The lysates were loaded twice on the column at a flow rate of 0.5 ml/min ...
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bioRxiv - Immunology 2023Quote: ... Binding responses were measured following immobilization by amine coupling of antigens (37) on CM5 sensor chips (BIAcore/GE Healthcare). Purified plasma IgG samples at 200 µg/ml were flowed (2.5 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gravity IMAC was performed by batch binding the clarified supernatants with 10mL of Ni Sepharose Excel resin (GE Healthcare). After 20-30 minutes of incubation ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then placed in 5% milk PBST plus 1:2500 rabbit specific horseradish peroxidase (HRP) antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then washed with TBS-T (3x, 5 min each) and incubated with horse radish peroxidase (HRP)-conjugated secondary antibodies (GE Healthcare). Following another wash step in TBS-T ...
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bioRxiv - Immunology 2019Quote: ... Membranes were blocked with 5% bovine serum albumin and incubated with the appropriate primary and HRP-conjugated secondary antibodies (GE Healthcare). Western Lightning ECL Pro (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were probed with ECL Plex Cy3- or Cy5-conjugated goat anti-mouse or anti-rabbit secondary antibodies (Cytiva) and scanned on an Amersham Typhoon 5 Phosphorimager (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... 5 % glycerol into two 5 mL MBP-trap cartridges (GE Healthcare) connected in tandem before removal of the Ni-NTA cartridges ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
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bioRxiv - Biochemistry 2019Quote: ... The template strand was purified by binding the biotinylated PCR product to Streptavidin Sepharose High Performance affinity resin (GE Healthcare), purification of the beads from the reaction mixture by repeated washing with Tris buffer (10 mM Tris HCl pH 7.5 ...
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bioRxiv - Bioengineering 2019Quote: The binding kinetics of Det7T with the microorganisms were studied using a BIAcore T200 instrument (GE Healthcare, Little Chalfont, UK). Det7T was diluted (1:50 ...
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bioRxiv - Immunology 2020Quote: ... The equilibrium Kd was determined by fitting the data to a steady-state 1:1 binding model using Biacore X100 Evaluation software version 2.0 (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... and those containing Tdp1 were pooled and concentrated to ~0.5 mL by binding to a small volume of SP sepharose resin (GE Healthcare) and elution with 50% buffer B ...
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bioRxiv - Cell Biology 2022Quote: ... Flow cell 1 was left empty to use this flow cell as a reference flow-cell for on-line subtraction of bulk solution refractive index and for evaluation of non-specific binding of analyte to the chip surface using Biacore T100 Control Software (version 3.2) (GE Healthcare). FPLC-purified non-biotinylated protein was used as analyte ...
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bioRxiv - Biophysics 2022Quote: ... recombinant proteins were purified twice by binding to and eluting from a Ni Sepharose 6 Fast Flow column (GE Healthcare) and a HiTrap Heparin HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: NAB2 binding to GST-Nedd4 and Nedd4(HECT) was characterized via SPR using the Biacore T200 instrument (GE Life Sciences). Recombinant protein was purified as described with an additional purification step using size exclusion chromatography to ensure high purity prior to use in SPR ...
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bioRxiv - Immunology 2020Quote: ... Background binding to blank immobilized flow cells was subtracted and affinity constants were calculated using BIA Evaluation software (GE Healthcare) using the Steady State Affinity model ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing tagged-SbtB7001 were pooled and transferred into binding buffer using a HiPrep 26/10 Desalting column (GE Healthcare). The His6–Ub tags were removed using a ubiquitin carboxyl-terminal hydrolase 2 catalytic core domain (USP2cc):SbtB7001 molar ratio of 1:25 at 4 °C overnight as previously described [14] ...
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bioRxiv - Microbiology 2020Quote: ... and the kinetic parameters were obtained by globally fitting the double-reference subtracted data to a 1:1 binding model with mass transport limitation using Biacore T200 Evaluation software v 3.1 (GE Healthcare). The dissociation rate constant (kd ...
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bioRxiv - Cell Biology 2020Quote: ... This was followed by performing SEC using GST binding buffer supplemented with 0.5 mM TCEP on a Superdex 200 10/300 column using an ÄKTA purifier (GE Healthcare). The fractions corresponding to the peak were pooled ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were then probed with HRP-conjugated anti-mouse IgG (Jackson Immunochemicals; 1:10,000) and binding was detected using ECL (GE Healthcare) in a LAS4000 instrument.
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bioRxiv - Biochemistry 2022Quote: ... fits of the sensorgrams were determined using a 1:1 binding model (Langmuir) using Biacore T200 Evaluation Software 3.1 (GE Healthcare).