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Citations for GE Life Sciences :
151 - 200 of 1074 citations for Recombinant Human V erb b2 Erythroblastic Leukemia Viral Oncogene Homolog avian His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... GST-tagged Keap1 was purified on glutathione-Sepharose 4B (GE Healthcare), eluted with 10 mM reduced glutathione in 50 mM Tris ...
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bioRxiv - Plant Biology 2021Quote: ... MBP tagged L7TIR was incubated with by PreScission protease (GE Healthcare) at 4°C or 22°C for 12 hr to remove the MBP tag ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged proteins were purified on Glutathione Sepharose resin (GE Healthcare) and eluted with buffer containing 20 mM reduced glutathione ...
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bioRxiv - Cell Biology 2022Quote: ... the GST-tagged protein was purified by Glutathione sepharose (GE Healthcare), followed by on-column TEV cleavage ...
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bioRxiv - Biochemistry 2023Quote: ... Crosslinked peptides were desalted and reconstituted with SEC buffer [30% (v/v) ACN in 0.1% (v/v) TFA] and fractionated using a Superdex Peptide PC 3.2/30 column (GE Healthcare). Collected fractions were analysed by liquid chromatography-coupled tandem mass spectrometry (MS/MS ...
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bioRxiv - Plant Biology 2019Quote: ... v/v/v) and the resolved lipids were visualized by phosphorimaging (Typhoon Trio Variable Mode Imager, GE Healthcare, Mississauga, Canada). The corresponding TAG spots were scraped and quantified for radioactivity using a LS 6500 multi-purpose scintillation counter (Beckman-Coulter ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Bioengineering 2023Quote: ... v/v) and loaded onto a HiTrap Capto Q column (GE Healthcare). The protein was further purified using a gradient of NaCl (0-1000 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... coli strain BL21 and purified using His-trap (GE Healthcare), Hi-Trap Q (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... was purified and washed on His-Trap columns (GE Healthcare) with buffer Ac (50 mM Tris pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... and purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... A His-Trap crude FF column (GE Healthcare Life Sciences) was used for this purification ...
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bioRxiv - Microbiology 2019Quote: ... soluble 6His-tagged proteins were purified using a NiNTA resine (GE Healthcare) and imidazole was removed by dialysis into a buffer containing 50 mM TrisHCl ...
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bioRxiv - Bioengineering 2021Quote: ... PEGylated mEGFP tagged ferritin was purified with PD10 desalting columns (GE Healthcare) equilibrated with 20mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... GST-tagged proteins were purified using Glutathione Sepharose 4B (GE Healthcare, USA). Protein concentration was determined using Bio-Rad protein assay (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... Histidine tagged proteins were immobilized on a Ni2+-sepharose resin (GE Healthcare) to remove the 1 mM DTT (Dithiothreitol ...
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bioRxiv - Microbiology 2020Quote: ... HisMBP-tagged PurR was loaded onto a HisTrap FF column (GE Healthcare) equilibrated with Lysis Buffer on an AktaPure FPLC (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10 % (v/v) fetal bovine serum (FBS) (GE Healthcare Life Sciences), 1 % (w/v ...
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bioRxiv - Immunology 2019Quote: ... Isotonic Percoll was prepared by mixing 90%v/v of Percoll (GE healthcare) with 9%v/v 10x HBSS and 1%v/v 1M HEPES pH 7.4 ...
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bioRxiv - Genomics 2020Quote: ... then replaced with complete MEM 10% (v/v) cosmic calf serum (GE Healthcare) and 1% penicillin/streptomycin antibiotics (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... 10% (v/v) glycerol) and loaded onto a heparin affinity column (GE healthcare) and eluted with 100% buffer D (buffer C + 1.5 M NaCl) ...
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bioRxiv - Bioengineering 2023Quote: ... v/v) and was loaded onto a HiTrap Capto Q column (GE Healthcare). Further purification involved a NaCl gradient (0–1000 mM ...
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bioRxiv - Neuroscience 2022Quote: ... Viral particles were purified using HiTrap heparin columns (GE HealthCare Life Science), concentrated with Amicon ultra-4 centrifugal filters to 100 µl and diluted with an equal volume of sterile phosphate buffered saline (PBS) ...
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bioRxiv - Biophysics 2020Quote: ... and affinity purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Equilibrated a 5 ml His-Trap HP column (GE Life Sciences) with the lysis buffer lacking protease inhibitors ...
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bioRxiv - Biochemistry 2022Quote: ... His-GST-ARS2 was applied onto a GST resin (GE Healthcare) and His-MBP-ZFC3H1 onto an Amylose resin (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE healthcare). Histones were eluted by a salt gradient from 200 mM to 600 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl of His Mag Sepharose® Ni beads (GE Healthcare) were equilibrated with equilibration buffer containing 20 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2022Quote: Non-acetylated CobB was purified on His-Trap columns (GE healthcare) as described above however ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the supernatants were mixed with His GraviTrap affinity column (GE Healthcare) and eluted in elution buffer (20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDwt or RBDmut proteins were equilibrated in PBS and kept at −20°C ...
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bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDs proteins were equilibrated in PBS and kept at -20°C.
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bioRxiv - Microbiology 2020Quote: ... The protein was captured using a His-Trap column (GE Healthcare). The column was washed with 10 CV’s of wash buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured on Nickel columns (His Trap HP, GE Healthcare) and eluted with 500 mM Imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE Healthcare). Histones were eluted by a salt gradient from 200 to 600 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% (v/v) fetal calf serum (FCSIII; GE Healthcare Life Sciences, HyClone), 2 mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Plant Biology 2021Quote: ... 100 mM DTT and 2% v/v IPG buffer pH 3– 10 (GE Healthcare) was applied to the strips ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 10% (v/v) fetal calf serum (FCSIII; GE Healthcare Life Sciences, HyClone), 2 mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... 0.005% v/v Surfactant P20 at 25 °C using the Biacore 8K (GE Healthcare), with a series S protein A sensor chip (GE Healthcare) ...
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bioRxiv - Physiology 2019Quote: ... GST-tagged protein from supernatant were purified using glutathione-Sepharose 4B (GE Healthcare). Sepharose bound proteins were used as the protein columns ...
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bioRxiv - Biochemistry 2021Quote: ... All other hexa-histidine-tagged proteins were purified using a HisTrap (GE Healthcare) affinity chromatography step followed by thrombin cleavage ...
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bioRxiv - Plant Biology 2022Quote: ... GST tagged proteins were purified with Glutathione Sepharose 4B beads (10250335, GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged Nlgn1- ECD were purified by rProteinA Sepharose Fast Flow (GE Healthcare), eluted with 10mM glycine ...
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bioRxiv - Cell Biology 2022Quote: ... tagged proteins were affinity-purified with a HisTrap HP nickel column (GE Healthcare) or glutathione Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... for the MBP-tagged proteins or glutathione sepharose resin (GE Healthcare Life Sciences) for the GST-tagged proteins ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged proteins were immobilized on glutathione sepharose beads (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Biochemistry 2022Quote: ... Tagged proteins were purified by affinity chromatography (HisTrap resp. StrepTrap, both GE Healthcare) with elution buffer containing 200 mM imidazole respectively 2.5 mM d-desthiobiotin ...
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bioRxiv - Cell Biology 2023Quote: GST-tagged proteins were affinity-purified using glutathione-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using glutathione agarose beads (GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (QIAGEN) ...