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Citations for GE Life Sciences :
151 - 200 of 806 citations for Recombinant Human CSNK1A1 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... and treated overnight with GST-PreScission Protease (GE Healthcare). Eluted proteins were further purified over a Superdex 75 16/600 pd column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... goat anti-GST HRP-conjugated antibody (RRID:AB_771429; GE Healthcare). Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was added GST agarose beads (GE Healthcare) and rotated at 8 °C for 1.5 hr ...
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bioRxiv - Microbiology 2021Quote: ... plates were incubated with goat anti-GST (GE Healthcare) for an hour and GST binding was detected kinetically using an alkaline-phosphatase-linked rabbit anti-goat IgG secondary antibody (diluted 1:2000 in 0.1% BSA/PBS) ...
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bioRxiv - Biochemistry 2021Quote: ... cleared lysate was applied on GST-Trap (GE Healthcare) before extensive washing with ly-sis buffer ...
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bioRxiv - Genetics 2023Quote: ... Glutathione SepharoseTM 4B (for GST fusions; GE Healthcare, 17075601) or amylose resin (for MBP fusions ...
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bioRxiv - Plant Biology 2023Quote: ... pGEX-2T (GE Healthcare, N-terminal fusion with GST) was used for CPK protein production in bacteria for previously reported constructs72 (CPK2/3/5/11) ...
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bioRxiv - Biochemistry 2024Quote: ... loaded onto 5 ml GST-trap column (GE Healthcare), equilibrated in buffer A ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare, Chicago, IL) at 4 °C for 2 h ...
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bioRxiv - Plant Biology 2020Quote: ... and Anti-GST-HRP conjugate (RPN1236; GE Healthcare Bio-Sciences) were used to detect MBP-SPA1 and GST or GST-PIF4 ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-KCTD19 was purified using Glutathione Sepharose 4B (GE Healthcare). The purified KCTD19 protein with a complete adjuvant was injected into female rats ...
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bioRxiv - Molecular Biology 2019Quote: ... the GST sequence from the pGEX-6P plasmid (GE Healthcare) was amplified using primers 5’-GGCAGCAGCCATCATCATCATCATC ACAGCAGCGGCCTGATGTCCCCTATACTAGGTTATTGG-3’ and 5’-TCTTCTTTTGGAACCCA TCATATGGCTGCCGCGCGGCACGGGCCCCTGGAACAGA-3’ ...
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bioRxiv - Cell Biology 2022Quote: Goat GST antibody (27457701V, GE Healthcare, used at 1:1,000), NeutrAvidin-DyLight 800 (22853 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were pooled onto GST affinity resin (GE Healthcare), incubated for 2 h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... The GST tag was cleaved with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Biophysics 2022Quote: ... the Glutathione Sepharose 4B affinity beads (GST beads) (GE Healthcare) were washed three times with buffer 25 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... which was applied on a GST-sepharose column (GE Healthcare). After washing with sonication buffer ...
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bioRxiv - Developmental Biology 2020Quote: GST fusion proteins were generated using pGEX5x.3 (GE Healthcare) as vector ...
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bioRxiv - Biochemistry 2021Quote: ... the GST eluent was incubated with Benzamidine-Sepharose (GE Healthcare) for 30 min at 4 °C.
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bioRxiv - Biochemistry 2021Quote: ... GST-Sds3 CTD was purified with glutathione sepharose (GE Healthcare) and eluted using 25 mM glutathione ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-TRIM41 was purified using Glutathione Sepharose 4B (GE Healthcare). The GST tag was removal by PreScission protease (27084301 ...
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bioRxiv - Molecular Biology 2023Quote: ... The GST tag was cleaved by PreScission Protease (GE Healthcare) treatment over 12 hrs at 4°C with the progress of reaction monitored by SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... GST-Rheb was cloned into pGEX (GE Healthcare Life Sciences). Silencer Select siRNAs of Rheb (s12019 ...
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bioRxiv - Cancer Biology 2021Quote: ... GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare) by rotating in binding buffer (50 mM Tris HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... On-column cleavage from the GST tag with Thrombin (GE Healthcare) was performed at 21 °C for 18 h in thrombin digestion buffer (20mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... PreScission protease was removed by binding to GST-Sepharose (GE Healthcare) for 2 h and buffer exchange with centrifugal filter devices (10,000 MWCO ...
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bioRxiv - Cell Biology 2020Quote: ... Cleared lysates were loaded onto GST-Trap (GE Healthcare Life Sciences) washed with 20-30 column volumes wash buffer (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Protein input was detected using an anti-GST antibody (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... the GST-tag was cleaved off using PreScission protease (GE Healthcare) in 50 mM Tris/HCl (pH8) ...
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bioRxiv - Biochemistry 2021Quote: ... GST fusion proteins were purified using Glutathione Sepharose HP (GE Healthcare) followed by cleavage with recombinant GST-HRV 3C protease and a GST recapture step ...
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bioRxiv - Molecular Biology 2022Quote: ... and GST (glutathione S-transferase) Sepharose beads (17075601, GE Life Sciences). Recombinant human HBP (2200-SE-050 ...
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bioRxiv - Neuroscience 2020Quote: ... GST protein was purified by adding Sepharose 4B beads (GE Healthcare) and incubating 2h at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... GST-BirA was removed afterwards with Glutathione Sepharose 4B (GE Healthcare) resin and excess biotin was separated from the labeled protein by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... GST fusion proteins were affinity-purified on glutathione-Sepharose (GE Healthcare). For in vitro pull-down assays ...
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bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removal by PreScission protease (27084301, GE Healthcare) and Glutathione Sepharose 4B affinity subtraction purification ...
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bioRxiv - Cell Biology 2020Quote: GST fusion proteins were cloned into pGEX6P1 (GE Healthcare, Pittsburgh, PA). MBP fusion proteins were cloned into pJP1.09 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the lysate was subjected to GST-affinity chromatography (GE Life Sciences), the GST tag was removed by PreScission protease ...
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bioRxiv - Cell Biology 2023Quote: ... The GST tag was cleaved off by PreScission protease (GE Healthcare). The GST tag of BAR domains did not inhibit liposome binding or tubulation ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-GST HRP-conjugated antibody (RRID:AB_771429; GE Healthcare; 1:10,000). Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Cell Biology 2019Quote: ... coli GST sequence on the pGEX-2T plasmid (GE Healthcare, Freiburg, Germany) using BamHI and EcoRI restriction sites ...
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bioRxiv - Cell Biology 2019Quote: ... except that the column was connected to a GST-Trap (GE Healthcare) for online removal of GST and the GST-3C protease.
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were coupled to glutathione sepharose (GE Healthcare, Freiburg, Germany) using 1x buffer B (10 mM HEPES-NaOH pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... goat polyclonal anti-GST (GE healthcare, 27-4577-01; 1:2000, IB); rabbit polyclonal anti-Cap1 [1 μg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... GST-fused proteins were purified using glutathione 9epharose 4B beads (GE HealthCare) equilibrated in 1x Phosphate Buffered Saline (PBS ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded onto a 4-mL GST column (GE Healthcare) equilibrated in PBS containing 1 mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were incubated with 250 μL of GST Sepharose 4B (GE Healthcare) for 3 hours at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... coli GST sequence on the pGEX-2T plasmid (GE Healthcare, Freiburg, Germany) using BamHI and EcoRI restriction sites ...
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bioRxiv - Cell Biology 2020Quote: ... The soluble fraction was bound to the GST Sepharose 4B (GE Healthcare) for 90 to 120 min at 4 °C ...