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Citations for GE Life Sciences :
151 - 200 of 863 citations for Puumala Virus Glycoprotein 2 Gc Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The GST-tag and Thrombin protease were removed via heparin affinity chromatography (HiTrap Heparin 5 ml FF, GE Healthcare). Cas12a was eluted via an elution gradient with 1 M NaCl (20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission, GE Healthcare). Fusion protease was removed using glutathione resin and the cleaved β2HA was collected in the flow-through ...
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bioRxiv - Biophysics 2021Quote: ... tag cleavage and dialysis followed by gel filtration on a Superdex 75 Hi Load column 16/60 (GE Healthcare) pre-equilibrated with 20mM Tris-HCl pH 8 ...
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bioRxiv - Evolutionary Biology 2023Quote: We performed size-exclusion chromatography of His-tag purified coGFP variants on an Äkta Pure chromatography system (GE Healthcare), with a Superdex 75 10/300 Increase column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Various Prp40 domains fused with an N-terminal Flag tag were cloned into pGEX-6p-1 vector (GE Healthcare) and purified from E.coli as GST fusion proteins ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Neuroscience 2020Quote: Binding kinetics of VNAR-Fcs was determined by surface plasmon resonance (SPR) using a Biacore T200 (GE Healthcare). A His-capture kit (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Fab fragments were purified by removing IgG and Fc contaminants using a HiTrap MabSelectSure (GE Healthcare Life Sciences) column according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Complexes were incubated with 10–15 μg of SARS-CoV-2 spike and ∼1 mg of purified polyclonal Fab ...
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bioRxiv - Immunology 2020Quote: ... and Fab fragments were purified from undigested IgG and Fc-fragments using SEC (Superdex 200 Increase; GE Healthcare). Final Fab yields were ~0.75-1.5 mg ...
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bioRxiv - Biophysics 2021Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody coupled Sepharose Beads (GE Healthcare) and eluted by removing the GFP tag with the PreScission Protease ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine plus MBP tag was removed by TEV protease digestion followed by a second step HisTrap column (GE Healthcare). Protein was further purified using Q and Superdex G75 columns (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... The cleaved linker and 6x-His tag were then removed using a His SpinTrap kit (GE Healthcare # 28-9321-71). Digested proteins in PBS were diluted 1:1 in Squalvax (Oz Biosciences # SQ0010 ...
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bioRxiv - Immunology 2019Quote: ... were captured via their histidine tags onto CM5 chips pre-coupled with ~9000 reflective units anti-His Ab (GE Healthcare) using standard amine chemistry ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C to remove the SBP-tag and polished by size exclusion chromatography on a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in 50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... The His6-tag-containing FdR9 was purified by affinity chromatography on an Äkta prime FPLC system (GE Healthcare, Freiburg, Germany), using a 5 mL HisTrap column (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged and the supernatant was filtered through a 0.45 μm filter and subjected to a His-tag affinity chromatography using a 5 mL HisTrapFF (GE Healthcare) and the ÄKTAprime plus system (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... The cleaved His6-MBP-tag was removed by gel filtration using a HiLoad 16/60 Superdex 200 column (GE healthcare) equilibrated in size exclusion buffer (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... the myosin was purified by His-tag affinity purification with a 1 ml HisTrap HP nickel-sepharose column (GE Healthcare). The eluted myosin solution was then dialyzed overnight at 4 °C in a low-salt buffer (25 mM imidazole pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... The tag-less protein was then purified by size-exclusion chromatography (SEC) using a Superdex 200 increase column (GE Healthcare) equilibrated with a buffer containing 25 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
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bioRxiv - Immunology 2020Quote: ... to remove the GST tag and further purified by gel filtration (SuperoseTM 6 prep grade XK 16/70; GE Healthcare). To prepare for crystallization trials ...
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bioRxiv - Genetics 2020Quote: ... followed by purification of the tag-free protein by anion exchange chromatography on a HiTrap Q XL column (GE Healthcare) and nickel affinity chromatography ...
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bioRxiv - Biophysics 2023Quote: ... The myosin was purified using His-tag affinity purification and anion-exchange purification by the AKTA purify system (GE Healthcare). Oligonucleotide labeling reactions were performed just after anion-exchange purification ...
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bioRxiv - Biophysics 2023Quote: ... Protein was purified via its C-terminal green fluorescence protein (GFP) tag using GFP nanobody-coupled Sepharose beads (GE Healthcare) and eluted by removing the GFP tag with PreScission Protease ...
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The eluted Cas1 without tag was concentrated and further purified by gel filtration chromatography (Superdex200 10/300 GL, GE Healthcare) in buffer B containing 10 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with trypsin to remove any virus that had not entered the cell and then cushioned onto Ficoll-Pacque (GE Healthcare Life Sciences) to remove residual virus and debris ...
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bioRxiv - Biochemistry 2021Quote: ... and the released virus particles were purified by 55% cesium chloride and desalted through a PD10 column (GE Healthcare Life Sciences, Cat#17085101). Adenovirus particles were tail vein injected at doses of 0.1 OD for the lean ...
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bioRxiv - Neuroscience 2023Quote: ... The adeno-associated virus (AAV) vectors were produced by helper-free triple transfection and purified using affinity chromatography (GE Healthcare, Chicago, IL, USA). For production of AAV2.1 vector ...
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bioRxiv - Molecular Biology 2023Quote: ... was processed for amplification of complete genome of the virus by rolling circle amplification (RCA) method using TempliPhi illustra amplification kit (GE Healthcare, Piscataway, NJ). The resulting RCA product (2 μL ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was added to lysed HEK cells prior to virus purification through heparin affinity columns (HiTrap Heparin HP, GE Healthcare, Uppsala, Sweden) and concentrated using Amicon Ultra-4 centrifugal filters (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Human cDNA of JAZF1 was purchased from GE Healthcare. Open reading frame (ORF ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Immunology 2021Quote: Human PBMCs were separated on Ficoll-Paque (GE Healthcare) by density centrifugation of heparinized blood from healthy donors (“Etablissement Français du Sang” EFS ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc fusion protein was purified from the cell culture supernatants with the protein A Sepharose (GE Healthcare). The purified ACE2-mouse IgG2a Fc fusion protein was biotinylated with Sulfo-NHS-LC-Biotin (Thermo) ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Biophysics 2021Quote: ... The protein samples without a His tag were purified by an anion-exchange chromatography (HiTrapQ HP 1 mL column, GE healthcare) followed by gel filtration chromatography (Superdex 75 10/300 GL column ...
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bioRxiv - Biophysics 2020Quote: ... Kif14 constructs were finally separated from the MBP tag by size-exclusion chromatography on a superpose 6 10/300 (GE Healthcare) column equilibrated with BRB80 buffer (80 mM Pipes (pH 6.8) ...
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bioRxiv - Bioengineering 2022Quote: The eGFP-AP fusion proteins and the eGFP-control were purified using the N-terminal His6-Tag and fast protein liquid chromatography (ÄKTAprime, GE Healthcare) with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid pGEX-dosR expressing DosR with an N-terminal GST-tag was generated by cloning PCR-derived dosR ORF fragment between BamHI and XhoI sites of pGEX 4T-1 (GE Healthcare) as described for pGEX-phoP (49) ...
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bioRxiv - Biophysics 2019Quote: ... 5 mg/mL of purified rPrP90-231 fused to an N-terminal six-histidine tag was immobilized in a nickel-loaded HisTrap HP affinity column (GE Healthcare). DNA library was added to the resin ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare). The target protein was further purified using MonoQ and Superdex G75 columns (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: ... The solubility tag was cleaved with TEV protease and the protein further purified by size exclusion chromatography (Superdex75 GL300/10/300; GE Healthcare) at 4°C in a SEC buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were digested with PreScission protease to remove tags and further purified by superose-6 increase column (GE Healthcare) in TBS buffer with 0.1% digitonin or 40 μM GDN ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were isolated from the medium exploiting the affinity of the 6xHis tag for the HisTrap Excel (GE Healthcare, USA) affinity column ...
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bioRxiv - Developmental Biology 2019Quote: Full-length dP75 protein with GST tag was purified from BL21 E.coli by using Glutathione Sepharose 4B GST-tagged protein purification resin (GE healthcare, 17075601) and following the protocol in GE healthcare webpage ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted proteins were then treated overnight with recombinant HRV 3C protease to remove the N-terminal His-tag and further purified via size exclusion chromatography on a HiLoad 26/600 Superdex 75 column (GE Healthcare) operating with 20 mM Tris-HCl ...